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Identification and Expression of PPAR in Sinonovacula constricta and Their Potential Regulatory Effects on Δ6 Fad Transcription

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Journal of Ocean University of China 2021年第6期20卷 1557-1566页

作者： RAN Zhaoshou KONG Fei LIAO Kai XU Jilin LIU Xingwang SHI Peng ZHANG Mengqi WU Kaibin YAN Xiaojun key laboratory of applied marine biotechnology Ningbo UniversityMinistry of Education of ChinaNingbo 315211China Collaborative Innovation Center for zhejiang marine High-Efficiency and Healthy Aquaculture Ningbo UniversityNingbo 315211China Fujian Baozhi Aquatic Science and Technology Co. Ltd.Zhangzhou 363503China National and Local Joint Engineering laboratory of marine biotechnology and Engineering Ningbo 315211China key laboratory of marine Biological Engineering Ningbo 315211China Evergreen Feed Co. Ltd.Ningbo 315211China

Peroxisome proliferator activated receptors(PPAR)are kinds of key transcriptional factors in regulating LC-PUFA *** now,little is known about PPAR in marine molluscs as well as in other *** constricta is the first marine mollusc that is proved to possess the complete LC-PUFA biosynthetic pathway,and it can be a perfect representative to clarify this *** this study,the molecular properties of *** PPAR were characterized,and corresponding potential regulatory roles in ***Δ6 fatty acyl desaturase(Fad)transcription were estimated by dual luciferase *** showed that two PPAR homologs(PPAR_a and_b)were identified in *** both contain typical features of vertebrate PPAR,suggesting highly conserved functional regions in *** phylogenetic comparison,they are clearly different with vertebrate PPAR and can be divided into two distinctive ***,they show a high expression level in gill,labial palps,mantle and *** down-regulated expressions in trochophore larva and veliger larva might be attributed to ***,the transcriptional activity of ***Δ6 Fad promoter was significantly activated by both PPAR_a and_b,indicating that *** PPAR might play a role in the regulation of LC-PUFA *** our knowledge,this is the first systematic analysis of PPAR in a marine *** results will provide a valuable reference for further researches on the function of marine molluscan PPAR and their underlying mechanisms in regulating LC-PUFA biosynthesis.

关键词： Sinonovacula constricta peroxisome proliferator activated receptors Δ6 fatty acyl desaturase long chain-polyunsaturated fatty acids

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Potential aquatic environmental risks of trifloxystrobin:Enhancement of virus susceptibility in zebrafish through initiation of autophagy

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Zoological Research 2021年第3期42卷 339-349页

作者： Huan Wang Tian-Xiu Qiu Jian-Fei Lu Han-Wei Liu Ling Hu Lei Liu Jiong Chen State key laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Ningbo UniversityNingboZhejiang 315211China laboratory of Biochemistry and Molecular Biology School of Marine SciencesMeishan CampusNingbo UniversityNingboZhejiang 315832China key laboratory of applied marine biotechnology of ministry of education Meishan CampusNingbo UniversityNingboZhejiang 315832China Ningbo Customs District Technology Center NingboZhejiang 315832China

Chronic pollution in aquatic ecosystems can lead to many adverse effects,including a greater susceptibility to pathogens among resident ***(TFS)is a strobilurin fungicide widely used in Asia to control soybean ***,it has the potential to enter aquatic ecosystems,where it may impair fish resistance to viral *** explore the potential environmental risks of TFS,we characterized the antiviral capacities of fish chronically exposed to TFS and subsequently infected with spring viraemia of carp virus(SVCV).Although TFS exhibited no significant cytotoxicity at the tested environmental concentrations during viral challenge,SVCV replication increased significantly in a time-dependent manner within epithelioma papulosum cyprini(EPC)cells and zebrafish exposed to 25μg/L *** showed that the highest viral load was more than 100-fold that of the *** biochemical assays indicated that autophagy was induced by TFS,and associated changes included an increase in autophagosomes,conversion of LC3-II,accumulation of Beclin-1,and degradation of P62 in EPC cells and *** addition,TFS markedly decreased the expression and phosphorylation of mTOR,indicating that activation of TFS may be associated with the mTORmediated autophagy *** study provides new insights into the mechanism of the immunosuppressive effects of TFS on non-target aquatic hosts and suggests that the existence of TFS in aquatic environments may contribute to outbreaks of viral diseases.

关键词： Trifloxystrobin Autophagy SVCV Chronic toxicity Susceptibility

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Analysis of DNA Methylation Level of Portunus trituberculatus Subjected to Low Salinity with Methylation-Sensitive Amplification Polymorphism

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Journal of Ocean University of China 2019年第5期18卷 1158-1162页

作者： LU Shaokun LI Ronghua GAO Tianlong WANG Chunlin ZHENG Weibing MU Changkao SONG Weiwei key laboratory of applied marine biotechnology of ministry of education Ningbo UniversityNingbo 315211China Collaborative Innovation Center for zhejiang marine High-Efficiency and Healthy Aquaculture Ningbo UniversityNingbo 315211China Third Institute of Oceanography State Oceanic AdministrationXiamen 361001China

In this study,the methylation-sensitive amplification polymorphism(MSAP)was used to compare the genomic DNA methylation level of muscle,gill and hepatopancreas of Portunus trituberculatus subjected to salinity 12 for 30 days to illustrate the epigenetic mechanism of *** primers were used to analyze the difference of methylation level of different *** results showed that the baseline methylation level of muscle,hepatopancreas and gill was 47.31%,22.94%and 17.69%,*** exposed to low salinity stress,the methylation epiloci changed in the three *** demethylation and methylation processes occurred under low salinity *** methylation ratio decreased in muscle and gill but increased in *** results indicated that DNA methylation is tissue-specific when *** responds to low salinity.

关键词： Portunus trituberculatus DNA methylation methylation-sensitive amplification polymorphism low salinity gill muscle hepatopancreas

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Hydroxycoumarin efficiently inhibits spring viraemia of carp virus infection in vitro and in vivo

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Zoological Research 2020年第4期41卷 395-409页

作者： Lei Liu Da-Wei Song Guang-Lu Liu Li-Peng Shan Tian-Xiu Qiu Jiong Chen State key laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Ningbo UniversityNingboZhejiang 315211China laboratory of Biochemistry and Molecular Biology School of Marine SciencesMeishan CampusNingbo UniversityNingboZhejiang 315832China key laboratory of applied marine biotechnology of ministry of education Meishan CampusNingbo UniversityNingboZhejiang 315832China School of Chemistry&Chemical Engineering Zhoukou Normal UniversityZhoukouHenan 466001China

Spring viremia of carp virus(SVCV)causes devastating losses in *** has an advantageous structure for the design of novel antiviral agents with high affinity and *** this study,we evaluated a hydroxycoumarin medicine,i.e.,7-(6-benzimidazole)coumarin(C10),regarding its anti-SVCV effects in vitro and in *** showed that up to 12.5 mg/L C10 significantly inhibited SVCV replication in the epithelioma papulosum cyprini(EPC)cell line,with a maximum inhibitory rate of>97%.Furthermore,C10 significantly reduced cell death and relieved cellular morphological damage in SVCV-infected *** mitochondrial membrane potential(ΔΨm)also suggested that C10 not only protected mitochondria,but also reduced apoptosis in SVCVinfected *** in vivo studies,intraperitoneal injection of C10 resulted in an anti-SVCV effect and substantially enhanced the survival rate of virusinfected ***,C10 significantly enhanced antioxidant enzyme activities and decreased reactive oxygen species(ROS)to maintain antioxidant-oxidant balance within the host,thereby contributing to inhibition of SVCV *** up-regulation of six interferon(IFN)-related genes also demonstrated that C10 indirectly activated IFNs for the clearance of SVCV in *** was beneficial for the continuous maintenance of antiviral effects because of the low viral loads in ***,C10 is suggested as a therapeutic agent with great potential against SVCV infection in aquaculture.

关键词： vivo thereby maintain

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Index of microcirculatory resistance: state-of-the-art and potential applications in computational simulation of coronary artery disease

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Journal of zhejiang University-Science B(Biomedicine & biotechnology) 2022年第2期23卷 123-140页

作者： Yingyi GENG Xintong WU Haipeng LIU Dingchang ZHENG Ling XIA key laboratory for Biomedical Engineering of ministry of education Institute of Biomedical EngineeringZhejiang UniversityHangzhou 310027China Research Centre of Intelligent Healthcare Faculty of Health and Life ScienceCoventry UniversityCoventry CV15FBUK

The dysfunction of coronary microcirculation is an important cause of coronary artery disease(CAD).The index of microcirculatory resistance(IMR)is a quantitative evaluation of coronary microcirculatory function,which provides a significant reference for the prediction,diagnosis,treatment,and prognosis of *** also plays a key role in investigating the interaction between epicardial and microcirculatory dysfunctions,and is closely associated with coronary hemodynamic parameters such as flow rate,distal coronary pressure,and aortic pressure,which have been widely applied in computational studies of ***,there is currently a lack of consensus across studies on the normal and pathological ranges of *** relationships between IMR and coronary hemodynamic parameters have not been accurately quantified,which limits the application of IMR in computational CAD *** this paper,we discuss the research gaps between IMR and its potential applications in the computational simulation of *** simulation based on the combination of IMR and other hemodynamic parameters is a promising technology to improve the diagnosis and guide clinical trials of CAD.

关键词： Index of microcirculatory resistance(IMR) Coronary artery disease(CAD) Computational simulation

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Enhancing production of a 24-membered ring macrolide compound by a marine bacterium using response surface methodology

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Journal of zhejiang University-Science B(Biomedicine & biotechnology) 2013年第4期14卷 346-354页

作者： Hua CHEN Mian-bin WU Zheng-jie CHEN Ming-lu WANG Jian-ping LIN Li-rong YANG key laboratory of Biomass Chemical Engineering of ministry of education Department of Chemical and Biological EngineeringZhejiang University zhejiang key laboratory of Antifungal Drugs

A 24-membered ring macrolide compound,macrolactin A has potential applications in pharmaceuticals for its anti-infectious and antiviral *** this study,macrolactin A was produced by a marine bacterium,which was identified as Bacillus subtilis by 16S ribosomal RNA(rRNA) sequence *** ionization mass spectrometry(ESI/MS) and nuclear magnetic resonance(NMR) spectroscopy analyses were used to characterize this *** improve the production,response surface methodology(RSM) involving Box-Behnken design(BBD) was *** bombycis,the main by-product in sericulture,was used as a nitrogen source in *** interactions between three significant factors,***,soluble starch,and(NH4)2SO4 were investigated.A quadratic model was constructed to fit the production and the *** medium composition was obtained by analysis of the *** cultivated in the optimum medium,the production of macrolactin A was increased to 851 mg/L,2.7 times as compared to the *** study is also useful to find another way in utilizing ***.

关键词： 24-membered ring macrolide Enhancing production Response surface methodology Faeces bombycis marine bacterium

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Recycling of ironmaking and steelmaking slags in Japan and China

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International Journal of Minerals,Metallurgy and Materials 2022年第4期29卷 739-749页

作者： Hiroyuki Matsuura Xiao Yang Guangqiang Li Zhangfu Yuan Fumitaka Tsukihashi Graduate School of Engineering The University of TokyoTokyo 113-8656Japan key laboratory of Coastal Environment and Resources of zhejiang province School of EngineeringWestlake UniversityHangzhou 310024China Institute of Advanced Technology Westlake Institute for Advanced StudyHangzhou 310024China key laboratory for Ferrous Metallurgy and Resources Utilization of ministry of education Wuhan University of Science and TechnologyWuhan 430081China Collaborative Innovation Center of Steel Technology University of Science and Technology BeijingBeijing 100083China The University of Tokyo Tokyo 113-8656Japan

The mass production of steel is inevitably accompanied by large quantities of *** treatment of ironmaking and steelmaking slags is a great challenge in the sustainable development of the steel *** and China are two major steel producing countries that have placed a large emphasis on developing new technologies to decrease slag emission or promote slag *** are almost completely reused or recycled in ***,due to stagnant infrastructural investments,future applications of slags in conventional sectors are expected to be *** new functions or applications of slags has become a research priority in *** example,the utilization of steelmaking slags in offshore seabeds to create marine forests is under *** is the top steel producer in the *** utilization ratios of ironmaking and steelmaking slags have risen steadily in recent years,driven largely by technological *** example,hot stage processing of slags for materials as well as heat recovery techniques has been widely applied in steel plants with good ***,increasing the utilization ratio of basic oxygen furnace slags remains a major *** innovations in slag recycling are crucial for the steel industries in Japan and ***,the current status and developing trends of utilization technologies of slags in both countries are reviewed.

关键词： ironmaking steelmaking slag recycling seawater stabilization

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Pseudomonas aeruginosa-induced mitochondrial dysfunction inhibits proinflammatory cytokine secretion and enhances cytotoxicity in mouse macrophages in a reactive oxygen species(ROS)-dependent way

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Journal of zhejiang University-Science B(Biomedicine & biotechnology) 2023年第11期24卷 1027-1036页

作者： Haitao YANG Yan WANG Hui FAN Feixue LIU Huimiao FENG Xueqing LI Mingyi CHU Enzhuang PAN Daoyang TENG Huizhen CHEN Jingquan DONG Jiangsu key laboratory of marine Bioresources and Environment Co-Innovation Center of Jiangsu Marine Bio-Industry TechnologyJiangsu Key Laboratory of Marine Pharmaceutical Compound ScreeningCollege of PharmacyJiangsu Ocean UniversityLianyungang222005China Department of Medicine laboratory the Second People’s Hospital of Lianyungang Affiliated to Kangda College of Nanjing Medical Universitythe Second People’s Hospital of Lianyungang CityLianyungang222000China Institute of Neuroscience the First People’s Hospital of LianyungangLianyungang222000China

Pseudomonas aeruginosa belongs to the genus Pseudomonas and is a common Gram-negative,exclusively aerobic,conditionally pathogenic bacterium with the characteristics of easy colonization,mutation,and multidrug resista... 详细信息

关键词： belongs reactive resistance

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Structural analysis and anti-complement activity of polysaccharides extracted from Grateloupia livida (Harv.) Yamada

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Journal of Oceanology and Limnology 2019年第3期37卷 806-814,I0001-I0002页

作者： ZHANG Wenjing JIN Weihua DUAN Delin ZHANG Quanbin key laboratory of Experimental marine Biology Institute of OceanologyChinese Academy of SciencesQingdao 266071China School of Medicine Zhejiang UniversityHangzhou 310058China College of biotechnology and Bioengineering Zhejiang University of TechnologyHangzhou 310014China laboratory for marine Biology and biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China State key Lab of Seaweed Bioactive Substances Qingdao 266000China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

Polysaccharides were extracted from Grateloupia livida(Harv.) Yamada using hot water(extracted product denoted WGW) and then degraded in dilute sulfuric acid(degraded product denoted WGWD). The degraded mixture was then separated into four fractions through anion exchange chromatography on 2-diethylaminoethanol(DEAE)-Bio-Gel Agarose FF gel. Electrospray ionization collision-induced dissociation tandem mass spectrometry(ESI-CID-MS/MS) was performed to elucidate the structural features of all fractions. In combination with nuclear magnetic resonance spectroscopy(NMR)and infrared spectroscopy(IR) data, the major polysaccharide structures were concluded to be μ-carrageenan and κ-carrageenan. μ-Carrageenan usually has a backbone of alternating 1,3-linked β-D-galactopyranose residues sulfated at C-4 and 1,4-linked a-D-galactopyranose residues sulfated at C-6, while κ-carrageenan consists of alternating 1,3-linked β-D-galactopyranose residues sulfated at C-4 and 1,4-linked a-D-3,6-anhydrogalactopyranose residues. Trace v-carrageenan, composed of 1,3-linked β-D-galactopyranose residues sulfated at C-4 and 1,4-linked a-D-galactopyranose residues sulfated at C-2 and C-6, was also detected. Furthermore, the polysaccharide had a backbone comprising 1,3-linked β-D-galactopyranose and1,4-linked α-L-galactopyranose sulfated at C-6, which is the agarose precursor. The hydroxy groups in the galactopyranose were partially substituted by methyl and pyruvic acid acetal(PA) groups. The anticomplementary activities of WGW and its derivatives against classical pathways were measured. The native polysaccharides in WGW had higher activities, while the derivatives had much weaker activities. This indicated that the molecular weight and sulfate content were important factors affecting the anti-complement activity.

关键词： red alga Grateloupia livida(Harv.)Yamada mass spectrometry structure

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Simultaneous removal of cadmium and sulfamethoxazole from aqueous solution by rice straw biochar

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Journal of zhejiang University-Science B(Biomedicine & biotechnology) 2013年第7期14卷 640-649页

作者： Xuan HAN Cheng-feng LIANG Ting-qiang LI Kai WANG Hua-gang HUANG Xiao-e YANG ministry of education key laboratory of Environmental Remediation and Ecological Health College of Environmental and Resource Sciences Zhejiang University School of marine Sciences Ningbo University Tobacco Production Technology Center Bijie Tobacco Company of Guizhou Province

The simultaneous sorption behavior and characteristics of cadmium (Cd) and sulfamethoxazole (SMX) on rice straw biochar were investigated. Isotherms of Cd and SMX were well modeled by the Langmuir equation (R2 >0.95). The calculated maximum adsorption parameter (Q) of Cd was similar in single and binary systems (34129.69 and 35919.54 mg/kg, respectively). However, the Q of SMX in a binary system (9182.74 mg/kg) was much higher than that in a single system (1827.82 mg/kg). The presence of Cd significantly promoted the sorption of SMX on rice straw biochar. When the pH ranged from 3 to 7.5, the sorption of Cd had the characteristics of a parabola pattern with maximum adsorption at pH 5, while the adsorption quantity of SMX decreased with increasing pH, with maximum adsorption at pH 3. The amount of SMX adsorbed on biochar was positively correlated with the surface area of the biochar, and the maximum adsorption occurred with d 250 biochar (biochar with a diameter of 150-250 μm). Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) showed that the removal of Cd and SMX by rice straw biochar may be attributed to precipitation and the formation of surface complexes between Cd or SMX and carboxyl or hydroxyl groups. The results of this study indicate that rice straw biochar has the potential for simultaneous removal of Cd and SMX from co-contaminated water.

关键词： Biochar Rice straw Simultaneous sorption Cadmium (Cd) Sulfamethoxazole (SMX)

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