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Direct and rapid detection of serum amino acid and monoamine neurotransmitters to assist the diagnosis of panic disorder

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Science China Chemistry 2025年第1期68卷 377-384页

作者： Shiwen Liu Xuerui Wan Meng Zhao Jiaqi Wang Weilan Wu Linlin You Yonggui Yuan Qian Xu Rong Gao key laboratory of Environmental Medicine Engineering Ministry of EducationSchool of Public HealthSoutheast UniversityNanjing 210009China key laboratory of Modern Toxicology of ministry of education Department of Hygienic Analysis and DetectionSchool of Public HealthNanjing Medical UniversityNanjing 211166China Department of Psychosomatics and Psychiatry Zhongda HospitalSchool of MedicineSoutheast UniversityNanjing 210009China

The measurement of amino acid and monoamine neurotransmitters(NTs)in serum plays a central role in the identification and monitoring of clinical panic disorder(PD).However,the strong polarity and chemical heterogeneity of NTs make direct extraction and rapid detection extremely ***,we developed a strategy that integrated solid phase extraction based on magnetic Fe_(3)O_(4)coated with polydopamine(Fe_(3)O_(4)@PDA)and direct analysis of mass spectrometry in real time(DART-MS)for quantification of 10 *** systematically investigated the optimal conditions of this strategy to achieve good accuracy and *** with existing magnetic solid phase extraction methods for NTs detection in biological samples,this strategy could adsorb more targets while having similar adsorption efficiencies,and the total process time was reduced by an average of 69.42%.We rapidly determined the metabolic abnormality of 6 types of NTs in the serum of PD patients and demonstrated their diagnostic *** addition,an outstanding feature of PD in which tryptophan was more metabolized by the KYN pathway and the 5-hydroxytryptamine pathway was inhibited could be elucidated by this *** results highlighted the potential ability of Fe_(3)O_(4)@PDA coupled with DART-MS to assist in the clinical diagnosis of PD.

关键词： neurotransmitters panic disorder magnetic solid phase extraction direct analysis of mass spectrometry in real time

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A novel and visible ratiometric fluorescence determination of carbaryl based on red emissive carbon dots by a solvent-free method

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Chinese Chemical Letters 2025年第2期36卷 314-318页

作者： Meiling Xu Xinyang Li Pengyuan Liu Junjun Liu Xiao Han Guodong Chai Shuangling Zhong Bai Yang Liying Cui College of Resources and Environment Jilin Agricultural University State key laboratory of Supramolecular Structure and Materials College of Chemistry Jilin University key laboratory of Straw Biology and Utilization The Ministry of Education Jilin Agricultural University

In this study, a simple and effective ratiometric fluorescence method has been developed for carbaryl detection, utilizing red emissive carbon dots（R-CDs）. The underlying principle of this proposed strategy relies on the rapid hydrolysis of carbaryl under an alkaline condition and production of 1-naphthol with blue-emission at 462 nm. Furthermore, the as-synthesized R-CDs（Em. 677 nm）, serve as a reference, enhancing the visual tracking of carbaryl through the transformation of fluorescent color from red to *** concentration of carbaryl exhibits a commendable linear correlation with the ratio of fluorescence intensity, ranging from 0 to 20 μg/m L（R2= 0.9989） with a low detection limit of 0.52 ng/m L. Additionally, the described methodology can be used for the enzyme-free visual assay of carbaryl, even in the presence of other carbamate pesticides and metal ions, in tap water and lake water samples with excellent accuracy（spiked recoveries, 94%–106.1%）, high precision(relative standard deviation（RSD） ≤ 2.42),and remarkable selectivity. This fast and highly sensitive naked-eye ratiometric sensor holds immense promise for carbaryl detection in intricate environments and food safety fields.

关键词： Carbaryl detection Carbon dots Fluorescence sensor Quantum yield Visual detection

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A Distance-Adjustable 3D DNA Scaff old Accelerated Quadruped DNA Walker for the Photoelectrochemical Biosensing of RNA in Phytophthora Infestans

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Journal of analysis and Testing 2025年第01期 71-81页

作者： Hui Yang Liying Ge Ziqing Liang Haoran Shen Yingju Liu Kaiyu He Weipeng Liu key laboratory for Biobased Materials and Energy of ministry of education College of Materials and EnergySouth China Agricultural University key laboratory of Information Traceability for Agricultural Products Ministry of Agriculture and Rural Aff airsInstitute of Agro-Product Safety and NutritionZhejiang Academy of Agricultural Sciences

Phytophthora infestans, the causative agent of potato late blight, poses a destructive threat to global agricultural production. Herein, a 3D DNA scaff old is elaborately designed as DNA tracks for accelerating the walking process of the quadruped walker, further accompanied by the perylene-3,4,9,10-tetracarboxylic dianhydride（PTCDA）/CdS/TiO2photoelectrode for the detection of RNA in Phytophthora infestans. Upon interaction with the target RNA, the quadruped walker was released to expeditiously walk along the 3D DNA scaff old and initiate the unfolding of hairpin 1（H1）, where H1 could completely pair with the alkaline phosphatase-modified hairpin 2（AP-H2）. Impressively, compared to traditional DNA track, the unique structure of the 3D DNA scaff old with equidistant binding sites and high continuity endowed the quadruped walker with a faster walking rate and better reaction efficiency. Consequently, a substantial quantity of AP-H2 was anchored, causing the hydrolysis of ascorbic acid 2-phosphate（AAP） into ascorbic acid（AA） by the immobilized AP. AA, as an active electron donor, can significantly increase the photocurrent signal. Thus, the proposed biosensor presents remarkable sensitivity in RNA detection with a detection limit down to 3.3 fM. This strategy of designing a 3D DNA scaff old as a DNA track provides a high-efficiency sensing platform and a valuable potential application for other biomarker detection.

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Integrated Sensing and Communication Enabled Sensing Base Station:System Design,Beamforming,Interference Cancellation and Performance analysis

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China Communications 2025年第1期22卷 111-127页

作者： Jiang Wangjun Wei Zhiqing Feng Zhiyong Chen Xu key laboratory of Universal Wireless Communications Ministry of EducationBeijing University of Posts and TelecommunicationsBeijing 100876China

This paper studies the sensing base station(SBS)that has great potential to improve the safety of vehicles and pedestrians on *** can detect the targets on the road with communication signals using the integrated sensing and communication(ISAC)*** with vehicle-mounted radar,SBS has a better sensing field due to its higher deployment position,which can help solve the problem of sensing blind *** this paper,key technologies of SBS are studied,including the beamforming algorithm,beam scanning scheme,and interference cancellation *** transmit and receive ISAC signals simultaneously,a double-coupling antenna array is *** free detection beam and directional communication beam are proposed for joint communication and sensing to meet the requirements of beamwidth and pointing *** joint timespace-frequency domain division multiple access algorithm is proposed to cancel the interference of SBS,including multiuser interference and duplex interference between sensing and ***,the sensing and communication performance of SBS under the industrial scientific medical power limitation is analyzed and *** results show that the communication rate of SBS can reach over 100 Mbps and the range of sensing and communication can reach about 500 m.

关键词： beamforming integrated sensing and communication(ISAC) interference cancellation performance analysis sensing base station

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Logically integrating exo/endogenous gated DNA trackers for precise micro RNA imaging via synergistic manipulation

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Chinese Chemical Letters 2025年第1期36卷 478-483页

作者： Fanghua Zhang Yuyan Li Hongyan Zhang Wendong Liu Zhe Hao Mingzheng Shao Ruizhong Zhang Xiyan Li Libing Zhang Tianjin key laboratory of Molecular Optoelectronic Sciences Department of ChemistryTianjin UniversityTianjin 300072China Institute of Photoelectronic Thin Film Devices and Technology Solar Energy Conversion CenterKey Laboratory of Photoelectronic Thin Film Devices and Technology of TianjinEngineering Research Center of Thin Film Photoelectronic Technology of Ministry of EducationNankai UniversityTianjin 300350China

Nanomaterials provide an ideal platform for biomolecular display due to their dimensions approach the molecular scale,facilitating binding behavior akin to that observed in solution-based *** nanoprobes hold great promise as miniature detectives capable of detecting mi RNAs within ***,current nanoprobes face a challenge in achieving the required precision for accurate miRNA detection,particularly within the intricate confines of the cellular microenvironment,due to interference with biological autofluorescence,off-target effects,and a lack of spatiotemporal ***,we have designed a dual-stimuli responsive DNA tracker,synergistically utilizing specific intracellular cues and external triggers,which enables spatiotemporal-controlled and precise detection and imaging of miRNAs"on demand".The tracker,which combines zeolitic imidazolate framework-67(ZIF-67)and unique hairpin DNA structures,effectively anchored onto the ZIF-67 through electrostatic interactions,remains in a dormant state until activated by abundant cellular ATP,resulting in the release of the hairpin structures that include a PC linker incorporated into the loop *** irradiation triggers specific recognition of the target *** newly developed HP-PC-BT@ZIF-67 tracker demonstrates precise spatiotemporal mi RNA detection and exhibits excellent biocompatibility,enabling specific mi RNA recognition"on demand"within cancer *** research presents a reliable mi RNA imaging platform in the intricate cellular environment,opening up the possibilities for precise biomedical analysis and disease diagnosis.

关键词： Stimuli-responsive DNA tracker Synergistic manipulation Spatiotemporal imaging microRNAs

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The ameliorative effects of ginger against heat-induced oxidative stress and mitochondrial dysfunction in Caenorhabditis elegans

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food Science and Human Wellness 2025年

作者： Tingting Xu Mingfang Tao Rong Li Shuxin Tang Yuting Huang Ting Wu Senem Kamiloglu Siyi Pan Xiaoyun Xu key laboratory of Environment Correlative Dietology (ministry of education)， Huazhong Agricultural University Hubei key laboratory of Fruit & Vegetable Processing & Quality Control， Huazhong Agricultural University College of food Science and Technology， Huazhong Agricultural University Department of food Engineering， Faculty of Agriculture， Bursa Uludag University Science and Technology Application and Research Center (BITUAM)， Bursa Uludag University

Ginger， rich in gingerols and shogaols， exhibits multiple biological properties. However， the mechanisms underlying its thermotolerance remain unclear. The study employed network pharmacology and experimental validation in Caenorhabditis elegans to investigate how gingerol-related compounds within ginger extract (GE) mitigated damage caused by heat stress (HS). A total of 18 types of gingerol analogues were identified in GE， among which 6-， 8-， and 10-gingerol， as well as 6-， 8-， and 10-shogaol were quantified. Collectively， these six compounds accounted for 54.4% of the total composition. Supplementation with 15 μg/mL GE significantly extended heat-stress lifespan by 20.30%， while the combination of the six major gingerols and shogaols at the same concentration prolonged lifespan by 18.93%. Additionally， pretreatment with GE and the combination alleviated HS-induced oxidative damage by eliminating reactive oxygen species (ROS) and upregulating antioxidant enzymes. Network pharmacology analysis suggested that the MAPK pathway may play a crucial role in thermotolerance. Experimental findings confirmed that ginger attenuated oxidative damage through the activation of SKN-1/Nrf2 and DAF-16/FOXO via the MAPK pathway. Moreover， GE stabilized mitochondrial membrane potential and restored ATP levels， thus preserving mitochondrial function during heat exposure. Further investigations using molecular docking and molecular dynamics simulations revealed that shogaols， with more stable binding affinities for KEAP1 protein， exhibited more potent effects than gingerols in prolonging lifespan and reducing ROS levels under HS conditions. In short， gingerol analogues from ginger conferred thermal resistance to nematodes by mitigating oxidative damage and mitochondrial dysfunction.

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Self-assembling chemically modified siRNA nanorings for RNAi therapy and neuroprotection in Alzheimer's disease

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Science China Chemistry 2025年

作者： Yifan Jiang Lisha Li Xiao Fang Tao Zeng Lichao Su Yichang Liu Ying Wu Jinyu Li Peng Lyu Zhonglei Lu Chunhua Lu Huanghao Yang New Cornerstone Science laboratory Ministry of Education Key Laboratory for Analytical Science of Food Safety and BiologyCollege of Chemistry Fuzhou University Fujian key laboratory of Medical analysis Fujian Academy of Medical Sciences Department of Neurosurgery Fujian Institute of Brain Disorders and Brain Science Fujian Clinical Research Center for Neurological Diseases First Affiliated Hospital Fujian Medical University National & Local Joint Biomedical Engineering Research Center on Photodynamic Technologies College of Chemistry Fuzhou University College of Biological Science and Technology Fuzhou University School of Medicine Fuzhou University

The development of RNA interference(RNAi) therapy offers a potential solution for Alzheimer's disease(AD). However, the brain-blood barrier(BBB) with its selective permeability and pharmacokinetic-related challenges poses restrictions on the delivery of small interfering RNA(siRNA) to the central nervous system(CNS). In this study, we demonstrate that the incorporation of 2'-fluoro(2'-F) substitutions and L-carnitine modification facilitates the self-assembly of siRNA through triple interaction, leading to the formation of nanorings, called LCSF-NR. Based on the enhanced cellular uptake and lysosomal escape by 2'-F substitution and the transport across the BBB promoted by L-carnitine, the nanorings realized the improved braintargeted delivery of siRNA, both in zebrafish and mice models. Moreover, our findings highlight the therapeutic potential of LCSF-NR formulation in an AD zebrafish model through a synergistic effect of downregulating the β-site APP cleavage enzyme1(BACE1) gene and L-carnitine-mediated neuroprotection, effectively inhibiting pathological processes. Overall, these results suggest that the chemical modification-based siRNA self-assembly strategy enables trans-BBB delivery and presents a concise approach for synergistic therapy of AD.

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Pattern-based rapid and robust profiling of gut microbiota and its metabolism with a single fluorescent probe

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Science China Chemistry 2025年第2期68卷 781-788页

作者： Hongli Tan Meizi Zeng Cong Fang Xiaohua Zhu Feng Liu Ying Long Ruijie Deng Peng Yin Meiling Liu Haitao Li Youyu Zhang Shouzhuo Yao key laboratory of Chemical Biology and Traditional Chinese Medicine Research(ministry of education) College of Chemistry and Chemical EngineeringInstitute of Interdisciplinary StudiesHunan Normal UniversityChangsha 410081China Hunan Cancer Hospital/The Affiliated Cancer Hospital of Xiangya School of Medicine Central South UniversityChangsha 410013China College of Biomass Science and Engineering Healthy Food Evaluation Research CenterSichuan UniversityChengdu 610064China

The gut microbiota and the associated metabolism play a pivotal role in maintaining human health,yet their assessment is challenging due to their inherent diversity,variety,and ***,we developed a multichannel sensor array based on a single fluorescent probe,allowing for rapid and robust profiling gut microbiota and their key metabolites including cysteine(Cys),glutathione(GSH),and homocysteine(Hcy).The assay leverages the single fluorescent probe that is with multiple binding sites and the cross-reactive sensing principle to specifically recognize different biological *** analyzing the pattern of biological thiols,the assay is capable of rapidly identifying six gut-derived bacteria including probiotics,neutral,and pathogenic strains based on fluorescent fingerprints within 5 min,and also discriminating bacteria and their mixtures with different *** the assay that enables the simultaneous measurement of multiple gut-derived bacteria and their metabolites,the designed array achieved an accuracy of 0.99 when discriminating colorectal cancer(CRC)patient feces samples from healthy ***,the as-prepared sensor array can also be used to identify various stages of *** simplicity,rapidness,and cost-effectiveness of our approach render it a robust platform for the analysis of gut microbiota.

关键词： gut microbiota biological thiols sensor array fluorescent probe

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Natural variations in a barley aldehyde oxidase 1 gene affect seed germination and malting quality

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The Crop Journal 2025年第1期13卷 299-303页

作者： Le Xu Peng Wang Xiaoqi Zhang Qisen Zhang Penghao Wang Yanhao Xu Chengdao Li Wenying Zhang MARA key laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River(Co-construction by ministry and Province) College of AgricultureEngineering Research Centre of Ecology and Agricultural Use of WetlandMinistry of EducationYangtze UniversityJingzhou 434023HubeiChina Western Barley Genetic Alliance Murdoch UniversityPerthWA 6150Australia Australian Export Grains Innovation Centre PerthWA 6151Australia Hubei key laboratory of food Crop Germplasm and Genetic Improvement Food Crops InstituteHubei Academy of Agricultural SciencesWuhan 430064HubeiChina

Multiple phytohormones,including gibberellin(GA),abscisic acid(ABA),and indole-3-acetic acid(IAA),regulate seed *** this study,a barley aldehyde oxidase 1(HvAO1)gene was identified,which is located near the SD2(seed dormancy 2)region at the telomeric end of chromosome 5H.A doubledhaploid population(AC Metcalfe/Baudin)was used to characterize HvAO1 and validated its association with seed germination and malting *** oxidase is predicted to catalyse the oxidation of various aldehydes,such as indoleacetaldehyde and abscisic aldehyde,into IAA and ABA,which is the final step of IAA/ABA *** process influences the final IAA/ABA concentration in the seed,affecting the seed *** analysis revealed substantial variations in the HvAO1 promoter regions between AC Metcalfe and *** combining seed germination tests,genetic variation analysis,gene expression,and phytohormone measurements showed that Baudin,which displays strong seed dormancy,has a specific sequence variation in the promoter region of the HvAO1 *** variation is associated with a higher expression level of the HvAO1 gene and an increased level of ABA than those in AC Metcalfe,which shows weak dormancy and lacks this sequence *** addition to its strong effect on the SD2 gene,HvAO1 shows excellent potential to fine-tune malting quality and seed dormancy,as evidenced by genotyping with HvAO1-specific markers,dormancy phenotypes,and malting *** findings provide a new strategy for introducing favourable HvAO1 alleles to achieve the desired level of seed dormancy and high malting quality in barley.

关键词： Barley(Hordeum vulgare L.) Dormancy Germination Malting quality Aldehyde oxidase

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Genome-wide association analysis reveals regulatory genes for the metabolite synthesis of 2-acetyl-1-pyrroline in aromatic coconut(Cocos nucifera L.)

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Journal of Genetics and Genomics 2025年第2期52卷 179-188页

作者： Hao Ding Xiang Lv Guangzhen Zhou Xiaomei Liu Xiwei Sun Jing Li Amjad Iqbal Yaodong Yang Hainan key laboratory of Tropical Oil Crops Biology/Coconut Research Institute Chinese Academy of Tropical Agricultural SciencesWenchangHainan 571339China Hainan Coconut International Joint Research Center WenchangHainan 571339China ministry of education key laboratory for Ecology of Tropical Islands Key Laboratory of Tropical Animal and Plant Ecology of Hainan ProvinceCollege of Life SciencesHainan Normal UniversityHaikouHainan 570228China Department of food Science&Technology Abdul Wali Khan University Mardan23200Pakistan

Coconut(Cocos nucifera L.)is a key tropical economic tree valued for its fruit flavor,particularly 2-acetyl-1-pyrroline(2AP),a vital aroma *** enhance high-aromatic coconut breeding efforts,it is essential to deeply understand the hereditary factors governing the production of *** this study,a genome-wide association analysis identifies 32 loci that exhibit significant associations with 2AP content based on single nucleotide polymorphism(SNP)variations from 168 aromatic coconut germplasm *** analysis then pinpoints 22 candidate genes near significant loci involved in 2AP *** encoded by these genes are involved in amino acid metabolism,glycolysis,and secondary *** these,Asparagine synthetase coding gene ASN1,Gamma-glutamylcysteine synthetase coding gene GSH1,and UbiA prenyltransferase coding gene UBIA are enriched in the linkage region constructed by significant locus Chr04_*** particular,the SNP mutation of CnASN1 leads to amino acid changes in the functional region of the coding protein,potentially resulting in differences in 2AP content among haplotype *** variations in related candidate genes,particularly the gene CnASN1,provides molecular markers closely associated with 2AP synthesis for coconut breeding and offers further insights into the metabolic mechanisms of 2AP.

关键词： Aromatic coconut 2AP SNP Genome-wideassociation study(GWAS) Haplotype analysis

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