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genetic dissection of the sensory and textural properties of Chinese white noodles using a specific RIL population

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Journal of Integrative Agriculture 2017年第2期16卷 454-463页

作者： LI Wen-jing DENG Zhi-ying CHEN Guang-feng CHEN Fang LI Xing-feng TIAN Ji-chun State key laboratory of Crop Biology Ministry of Science and Technology/Key Laboratory of Crop Biology of Shandong Province/Agronomy CollegeShandong Agricultural University College of Ecology and Garden Architecture Dezhou University

To dissect the genetic control of the sensory and textural quality traits of Chinese white noodles, a population of recom- binant inbred lines （RILs）, derived from the cross of waxy wheat Nuomai 1 （NM1） and Gaocheng 8901 （Gc8901）, was used. The RILs were tested in three different environments to determine the role of environmental effects on quantitative trait loci （QTL） analysis. A total of 45 QTLs with additive effects for 17 noodle sensory and textural properties under three environments were mapped on 15 chromosomes. These QTLs showed 4.23-42.68% of the phenotypic variance explained （PVE）. Nineteen major QTLs were distributed on chromosomes 1 B, 1 D, 2A, 3B, 3D, 4A, and 6A, explaining more than 10% of the phenotypic variance （PV）. Clusters were detected on chromosomes 2B （3 QTLs）, 3B （11 QTLs） and 4A （5 QTLs）. The cluster detected on chromosome 4A was close to the Wx-B1 marker. Five co-located QTLs with additive effects were identified on chromosomes 2B, 3D, 4A, 6A, and 7B. The two major QTLs, ***-3B. 1 and ***-3B. 1, in cluster wPt666008-wPt5870 on chromosome 3B were detected in three different environments, which perhaps can be directly applied to improve the textural properties of noodles. These findings could offer evidence for the selection or development of new wheat varieties with noodle quality using molecular marker-assisted selection （MAS）.

关键词： wheat (Triticum aestivum L.) noodle sensory quality textural property QTL

Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean

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Journal of Integrative Agriculture 2016年第10期15卷 2228-2236页

作者： CHENG Wen GAO Jin-shan FENG Xing-xing SHAO Qun YANG Su-xin FENG Xian-zhong key laboratory of Systems Biology in Universities of shandong/College of Life Science Shandong Normal University key laboratory of Soybean Molecular Design Breeding Northeast Institute of Geography and Agroecology Chinese Academy of Sciences

Plant height is one of the most important traits in soybean. The semi-dwarf soybean cultivars could improve the ability of lodging resistance to obtain higher yield. To broaden the dwarfism germplasm resources in soybean, 44 dwarf mutants were identified from a gamma rays mutagenized M-2 population. Two of these mutants, Gmdwf1（Glycine max dwarf 1） and Gmdwf2（Glycine max dwarf 2）, were investigated in this study. genetic analysis showed that both mutants were inherited in a recessive manner and their mutated regions were delimited to a 2.610-Mb region on chromosome 1 by preliminary mapping. Further fine mapping study proved that the two mutants had a common deletion region of 1.552 Mb in the target region, which was located in a novel locus site without being reported previously. The dwarfism of Gmdwf1 could not be rescued by gibberellin（GA） and brassinolide（BR） treatments, which indicated that the biosynthesis of these hormones was not deficient in Gmdwf1.

关键词： soybean dwarf mutant mapping BR GA

Proteo-transcriptomic profiles reveal key regulatory pathways and functions of LDHA in the ovulation of domestic chickens(Gallus gallus)

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1449-1465页

作者： Ruixue Nie Wenhui Zhang Haoyu Tian Junying Li Yao Ling Bo Zhang Hao Zhang Changxin Wu State key laboratory of Animal Biotech Breeding Beijing Key Laboratory for Animal Genetic ImprovementCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Background In poultry, the smooth transition of follicles from the preovulatory-to-postovulatory phase impacts egg production in hens and can benefit the poultry industry. However, the regulatory mechanism underlying follicular ovulation in avians is a complex biological process that remains *** Critical biochemical events involved in ovulation in domestic chickens(Gallus gallus) were evaluated by transcriptomics, proteomics, and in vitro assays. Comparative transcriptome analyses of the largest preovulatory follicle(F1) and postovulatory follicle(POF1) in continuous laying(CL) and intermittent laying(IL) chickens indicated the greatest difference between CL_F1 and IL_F1, with 950 differentially expressed genes(DEGs), and the smallest difference between CL_POF1 and IL_POF1, with 14 DEGs. Additionally, data-independent acquisition proteomics revealed 252 differentially abundant proteins between CL_F1 and IL_F1. Perivitelline membrane synthesis, steroid biosynthesis, lysosomes, and oxidative phosphorylation were identified as pivotal pathways contributing to ovulation regulation. In particular, the regulation of zona pellucida sperm-binding protein 3, plasminogen activator, cathepsin A, and lactate dehydrogenase A(LDHA) was shown to be essential for ovulation. Furthermore, the inhibition of LDHA decreased cell viability and promoted apoptosis of ovarian follicles in *** This study reveals several important biochemical events involved in the process of ovulation, as well as crucial role of LDHA. These findings improve our understanding of ovulation and its regulatory mechanisms in avian species.

关键词： Chicken Data-independent acquisition proteomics LDHA Ovulation Regulatory mechanism Transcriptome

Characterization and expression analysis of a novel RING-HC gene,ZmRHCP1,involved in brace root development and abiotic stress responses in maize

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Journal of Integrative Agriculture 2017年第9期16卷 1892-1899页

作者： LI Wen-lan SUN Qi LI Wen-cai YU Yan-li ZHAO Meng MENG Zhao-dong Maize Research Institute Shandong Academy of Agricultural Sciences~National Engineering Laboratory of Wheat and Maize/Key Laboratory of Biology and Genetic Improvement of Maize in Northern Yellow-Huai River PlainMinistry of AgricultureJinan 250100.P.R.China

RING is a really interesting new gene which plays important regulatory roles in many developmental processes as well as in plant-environment interactions. In the present report, the Zm RHCP1 gene encoding a putative RING-HC protein was isolated from maize and characterized. The Zm RHCP1 protein contained 310 amino acid residues with a conserved RINGHC zinc-finger motif and two transmembrane（TM） domains. Zm RHCP1 was expressed ubiquitously in various organs（root, stem, leaf, seedling, immature ear, and tassel）, but its transcript levels were higher in vegetative organs than in reproductive organs. Moreover, the expression pattern of Zm RHCP1 in brace roots indicated that Zm RHCP1 functions in brace root initiation. In addition, Zm RHCP1 expression was regulated by abiotic stresses. The expression results suggested that Zm RHCP1 plays important roles in brace root development and abiotic stress responses. The findings of the present study provide important information to help us understand the function of Zm RHCP1 in maize.

关键词： RING-HC zinc-finger brace root expression abiotic stresses maize

Heterologous expression of the ThIPK2 gene enhances drought resistance of common wheat

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Journal of Integrative Agriculture 2020年第4期19卷 941-952页

作者： ZHANG Shu-juan LI Yu-lian SONG Guo-qi GAO Jie ZHANG Rong-zhi LI Wei CHEN Ming-li LI Gen-ying Crop Research Institute Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River ValleyMinistry of Agriculture/National Engineering Laboratory for Wheat and MaizeJinan 250100P.R.China

Th IPK2 is an inositol polyphosphate kinase gene cloned from Thellungiella halophila that participates in diverse cellular processes. Drought is a major limiting factor in wheat(Triticum aestivum L.) production. The present study investigated whether the application of the Th IPK2 gene could increase the drought resistance of transgenic wheat. The codon-optimized Th IPK2 gene was transferred into common wheat through Agrobacterium-mediated transformation driven by either a constitutive maize ubiquitin promoter or a stress-inducible rd29 A promoter from Arabidopsis. Molecular characterization confirmed the presence of the foreign gene in the transformed plants. The transgenic expression of Th IPK2 in wheat led to significantly improve drought tolerance compared to that observed in control plants. Compared to the wild type(WT) plants, the transgenic plants showed higher seed germination rates, better developed root systems, a higher relative water content(RWC) and total soluble sugar content, and less cell membrane damage under drought stress conditions. The expression profiles showed different expression patterns with the use of different promoters. The codon-optimized Th IPK2 gene is a candidate gene to enhance wheat drought stress tolerance by genetic engineering.

关键词： codon-optimized drought rd29A ThIPK2 Triticum aestivum L.

Chromosomal Constitutions and Reactions to Powdery Mildew and Stripe Rust of Four Novel Wheat-Thinopyrum intermedium Partial Amphiploids

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Journal of genetics and Genomics 2014年第12期41卷 663-666页

作者： Yinguang Bao Xia Wu Chao Zhang Xingfeng Li Fang He Xiaolei Qi Honggang Wang shandong key laboratory of Crop Biology State Key Laboratory of Crop Biology Shandong Agricultural University Tai'an Subcentre of National Wheat Improvement Centre Agronomy CollegeShandong Agricultural University

As a staple food crop for one-third of the world＇s population, common wheat （Triticum aestivum L., 2n = 6x = 42, AABBDD） plays an important role in humans＇ food security. However, the genetic variation of cultivated wheat has been dramatically narrowed by genetic erosion under the modem cultivation system, resulting in vulnerability to biotic and abiotic stresses （Jiang et al., 1994; Friebe et al., 1996）. The wild relatives of wheat represent a large reservoir of superior genes, and transferring these alien genes to modem cultivars through chromosome engineering is a successful method of broadening the genetic diversity of wheat （Chen et al., 2003;

关键词： Th TE Chromosomal Constitutions and Reactions to Powdery Mildew and Stripe Rust of Four Novel Wheat-Thinopyrum intermedium Partial Amphiploids St

Dynamic genetic analysis for body weight and main length ratio in turbot Scophthalmus maximus

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Acta Oceanologica Sinica 2020年第2期39卷 22-27页

作者： Xin’an Wang Aijun Ma Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences/Shandong Province Key Laboratory of Marines Fisheries Biotechnology and Genetic BreedingQingdao 266071China laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

The objective of this study was to estimate genetic parameters of body width(BW)to body length(BL)ratio(BW/BL)and of body weight traits(BWT)in turbot,and to elucidate the genetic mechanism of the two traits during ontogeny by dynamic genetic *** 3 to 27 months,BW,BL and BWT of each communally stocked fish were measured every 3 *** BW/BL ratio was measured at different sampling ages.A twotrait animal model was used for genetic evaluation of *** results showed that the heritability values of BW/BL ratio ranged from 0.2168 to 0.3148,corresponding to moderate *** BWT heritability values ranged from 0.2702 to 0.3479 corresponding to moderate *** heritability of BW/BL ratio was lower than that of BWT,except at 3 months of *** correlation between BW/BL ratio and BWT decreased throughout the measurement *** correlations were higher than the phenotypic *** current results for estimating genetic parameters demonstrate that the BW/BL ratio could be used as a phenotypic marker of fast-growing turbot,and the BW/BL ratio and BWT could be improved simultaneously through selective breeding.

关键词： turbot dynamic genetic analysis body weight main length ratio heritability genetic correlation

QTL Detection and Epistasis Analysis for Heading Date Using Single Segment Substitution Lines in Rice(Oryza sativa L.)

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Journal of Integrative Agriculture 2014年第11期13卷 2311-2321页

作者： LI Guang-xian CHEN Ai-hua LIU Xu WANG Wen-ying DING Han-feng LI Jun LIU Wei LI Si-shen YAO Fang-yin State key laboratory of Crop Biology Shandong Agricultural University shandong Rice Research Institute Bio-Tech Research Center Shandong Academy of Agricultural Sciences

Heading date of rice is a key agronomic trait determining cultivated areas and seasons and affecting yield. In the present study, ifve primary single segment substitution lines with the same genetic background were used to detect quantitative trait loci （QTLs） for heading date in rice. Two QTLs, qHD3 and qHD6 on the short arm of chromosome 3 and the short arm of chromosome 6, respectively, were identiifed under natural long-day （NLD）. Nineteen secondary single segment substitution lines （SSSLs） and seven double segments pyramiding lines were designed to map the two QTLs and to evaluate their epistatic interaction between them. By overlapping mapping, qHD3 was mapped in a 791-kb interval between SSR markers RM3894 and RM569 and qHD6 in a 1 125-kb interval between RM587 and RM225. Results revealed the existence of epistatic interaction between qHD3 and qHD6 under natural long-day （NLD）. It was also found that qHD3 and qHD6 had signiifcant effects on plant height and yield traits, indicating that both of the QTLs have pleiotropic effects.

关键词： rice (Oryza sativa L.) single segment substitution lines heading date epistasis

Development of an Agrobacterium-mediated CRISPR/Cas9 system in pea(Pisum sativum L.)

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The Crop Journal 2023年第1期11卷 132-139页

作者： Guan Li Rong Liu Rongfang Xu Rajeev KVarshney Hanfeng Ding Mengwei Li Xin Yan Shuxian Huang Juan Li Dong Wang Yishan Ji Chenyu Wang Junguang He Yingfeng Luo Shenghan Gao Pengcheng Wei Xuxiao Zong Tao Yang National key Facility for Crop Gene Resources and genetic Improvement/Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China key laboratory of Rice genetic Breeding of Anhui Province Rice Research InstituteAnhui Academy of Agricultural SciencesHefei 230031AnhuiChina State Agricultural Biotechnology Centre Centre for Crop and Food InnovationFood Futures InstituteMurdoch UniversityMurdochWA 6150Australia Institute of Crop Germplasm Resources Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Crop Genetic ImprovementEcology and PhysiologyJinan 250100ShandongChina Zhejiang Xinnan Chemical Industrial Group Co. Ltd.Hangzhou 311300ZhejiangChina State key laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China

Pea(Pisum sativum L.)is an annual cool-season legume *** to its role in sustainable agriculture as both a rotation and a cash crop,its global market is expanding and increased production is urgently *** both technical and regulatory reasons,neither conventional nor transgenic breeding techniques can keep pace with the demand for increased *** answer to this challenge,CRISPR/Cas9 genome editing technology has been gaining traction in plant biology and crop breeding in recent ***,there are currently no reports of the successful application of the CRISPR/Cas9 genome editing technology in *** developed a transient transformation system of hairy roots,mediated by Agrobacterium rhizogenes strain K599,to validate the efficiency of a CRISPR/Cas9 *** optimization resulted in an efficient vector,*** used this optimized CRISPR/Cas9 system to edit the pea phytoene desaturase(PsPDS)gene,causing albinism,by Agrobacterium-mediated genetic *** is the first report of successful generation of gene-edited pea plants by this route.

关键词： Agrobacterium-mediated transformation CRISPR/Cas9 Pea Genome editing