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Transcriptional regulation of MdPIN7 by MdARF19 during gravityinduced formation of adventitious root GSA in self-rooted apple stock

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Horticultural Plant Journal 2024年第5期10卷 1073-1084页

作者： Zenghui Wang Xuemei Yang Linyue Hu Wei Liu Lijuan Feng Xiang Shen Yanlei Yin Jialin Li shandong Institute of Pomology Tai'anShandong 271000China School of Biological Science and Technology University of JinanJinanShandong 250022China State key laboratory of Crop Biology Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Huanghuai Region)Ministry of AgricultureCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai'anShandong 271018China

Self-rooted apple stock is widely used for apple ***,the shallowness of the adventitious roots in self-rooted apple stock leads to poor performance in the barren orchards of *** is because of the considerable difference in the development of a gravitropic set-point angle(GSA)between self-rooted apple stock and seedling ***,it is crucial to study the molecular mechanism of adventitious root GSA in self-rooted apple stock for breeding self-rooted and deep-rooted apple rootstock *** apple auxin response factor MdARF19 functioned to establish the adventitious root GSA of self-rooted apple stock in response to gravity and auxin ***19 bound directly to the MdPIN7 promoter,activating its transcriptional expression and thus regulating the formation of the adventitious root GSA in 12-2 self-rooted apple ***,MdARF19 influenced the expression of auxin efflux carriers(MdPIN3 and MdPIN10)and the establishment of adventitious root GSA of self-rooted apple stock in response to gravity signals by direct activation of *** findings provide new information on the transcriptional regulation of MdPIN7 by auxin response factor MdARF19 in the regulation of the adventitious root GSA of self-rooted apple stock in response to gravity and auxin signals.

关键词： Apple Self-rooted stock Gravity MdARF19 MdPIN7 Gravitropic set-point angle Transcriptional regulation

Applications of CRISPR-Cas9 mediated genome engineering

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Journal of Medical Colleges of PLA(China) 2015年第1期30卷 11-17页

作者： Xiao Yang State key laboratory of Proteomics Collaborative Innovation Center for Cardiovascular Disorders Genetic Laboratory of Development and DiseaseInstitute of Biotechnology

Targeted mutagenesis based on homologous recombination has been a powerful tool for understanding the mechanisms underlying development, normal physiology, and disease. A recent breakthrough in genome engineering technology based on the class of RNA-guided endonucleases, such as clustered regularly interspaced short palindromic repeats(CRISPR)-associated Cas9, is further revolutionizing biology and medical studies. The simplicity of the CRISPR-Cas9 system has enabled its widespread applications in generating germline animal models, somatic genome engineering, and functional genomic screening and in treating genetic and infectious diseases. This technology will likely be used in all fields of biomedicine, ranging from basic research to human gene therapy.

关键词： CRISPR-Cas9 Genome editing Functional genomic screening Gene therapy Animal model

Establishment and evaluation of a peanut association panel and analysis of key nutritional traits

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Journal of Integrative Plant Biology 2018年第3期60卷 195-215页

作者： Xiurong Zhang Suqing Zhu Kun Zhang Yongshan Wan Fengzhen Liu Qingfang Sun Yingjie Li State key laboratory of Crop Biology Shandong Key Laboratory of Crop BiologyShandong Agricultural UniversityTai'an 271018China

Breeding programs aim to improve the yield and quality of peanut （Arachis hypogaea L.）; using association mapping to identify genetic markers linked to these quantitative traits could facilitate selection efficiency. A peanut association panel was established consisting of 268 lines with extensive phenotypic and genetic variation, meeting the requirements for associa- tion analysis. These lines were grown over 3 years and the key agronomic traits, including protein and oil content were examined. Population structure （Q） analysis showed two subpopulations and clustering analysis was consistent with Q-based membership assignment and closely related to botanical type. Relative Kinship （K） indicated that most of the panel members have no or weak familial related- ness, with 52.78% of lines showing K=o. Linkagedisequilibrium （LD） analysis showed a high level of LD occurs in the panel. Model comparisons indicated false positives can be effectively controlled by taking Q and K into consideration and more false positives were generated by K than Q. A preliminary association analysis using a Q＋ K model found markers significantly associated with oil, protein, oleic acid, and linoleic acid, and identified a set of alleles with positive and negative effects. These results show that this panel is suitable for association analysis, providing a resource for marker-assisted selection for peanut improvement.

关键词： Georg Jander Cornell University USA

SHANK1 and autism spectrum disorders

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Science China(Life Sciences) 2015年第10期58卷 985-990页

作者： GONG XiaoHong WANG HongYan MOE key laboratory of Contemporary Anthropology and State key laboratory of genetic Engineering School of Life SciencesFudan University

Autism spectrum disorders （ASD） are highly heterogeneous pediatric developmental disorders with estimated heritability more than 70%. Although the genetic factors in ASD are mainly unknown, a large number of gene mutations have been found, especially in genes involved in neurogenesis. The Neurexin-Neuroligin-Shank （NRXN-NLGN-SHANK） pathway plays a key role in the formation, maturation and maintenance of synapses, consistent with the hypothesis of neurodevelopmental abnormality in ASD. Presynaptic NRXNs interact with postsynaptic NLGNs in excitatory glutamatergic synapses. SHANK proteins function as core components of the postsynaptic density （PSD） by interacting with multiple proteins. Recently, deletions and point mutations of the SHANK1 gene have been detected in ASD individuals, indicating the involvement of SHANK1 in ASD. This review focuses on the function of SHANK1 protein, Shankl mouse models, and the molecular genetics of the SHANK1 gene in human ASD.

关键词： autism spectrum disorders SHANK1 synapse genetics mouse model

Calcium Homeostasis in Parkinson’s Disease:From Pathology to Treatment

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Neuroscience Bulletin 2022年第10期38卷 1267-1270页

作者： Jingxian Zhang Qingqing Shen Yue Ma Lin Liu Wenting Jia Leilei Chen Junxia Xie Institute of Brain Science and Disease Shandong Provincial Collaborative Innovation Center for Neurodegenerative DisordersShandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological DisordersQingdao UniversityQingdao266071China

Disturbances of Ca^(2+)homeostasis have been reported to be associated with the development of Parkinson's disease(PD)(Fig.1),which is characterized by the selective vulnerability of dopaminergic neurons in the substantia nigra pars compacta(SNpc).Different from the dopaminergic neurons in the ventral tegmental area(VTA),which mainly rely on hyperpolarization-activated cyclic nucleotide-gated/voltagegated channels for pacing and have a strong Ca^(2+)-buffering capacity.

关键词： homeostasis stasis capacity.

Effects of selenium and sulfur on antioxidants and physiological parameters of garlic plants during senescence

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Journal of Integrative Agriculture 2016年第3期15卷 566-572页

作者： CHENG Bo LIAN Hai-feng LIU Ying-ying YU Xin-hui SUN Ya-li SUN Xiu-dong SHI Qing-hua LIU Shi-qi State key laboratory of Crop Biology Ministry of Science and Technology/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huanghuai Region) Ministry of Agriculture/College of Horticultural Science and Engineering Shandong Agricultural University Institute of Science and Technology Information Shandong Academy of Agricultural Sciences

A hydroponic study was conducted to determine the effects of selenium（Se： 0, 3, 6 μmol L^-1） on senescence-related oxidative stress in garlic plants grown under two sulfur（S） levels. We evaluated the yields of plants harvested at 160 and 200 days after sowing. Plants grown under a low Se dose（0.3 μmol L^-1） at low S level showed higher yields（12.0% increase in fresh weight yield, 13.7% increase in dry weight yield） than the controls, despite a decrease in chlorophyll concentration. Compared with control plants, the Se-treated plants showed lower levels of lipid peroxidation. The Se-treated plants also showed higher activities of glut athione peroxidase and catalase, but lower superoxide dismutase activities. Changes in Fv/Fm values and proline contents were affected more strongly by S than by Se. On the basis of our results, we can conclude that Se plays a key role in the antioxidant systems in garlic seedlings. It delays senescence by alleviating the peroxide stress, but it can be toxic at high levels. A high S level may increase tolerance to high Se concentrations through reducing Se accumulation in plants.

关键词： selenium sulfur garlic antioxidant activity oxidative stress senescence

Melatonin Treatment Enhances the Polyphenol Content and Antioxidant Capacity of Red Wine

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Horticultural Plant Journal 2018年第4期4卷 144-150页

作者： XU Lili YUE Qianyu BIAN Feng'e ZHAI Heng YAO Yuxin State key laboratory of Crop Biology Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huang-Huai Region Ministry of Agriculture) College of Horticulture Science and Engineering Shandong Agricultural University

Melatonin and polyphenols are strong antioxidants, and melatonin is also a potential plant growth regulator. The role of melatonin in regulating polyphenol metabolism is unclear. This study assessed the primary impacts of exogenous melatonin treatment on phenolics accumulation and antioxidant capacity of the 'Moldova' wine. It was found that two times of 100 μmol·L^(-1) melatonin treatment clearly enhanced the endogenous melatonin content of ripened berries and wine. Further experiments indicated that melatonin treatment significantly increased the contents of total phenols, flavonoids and anthocyanins in wine. Additionally, the contents of most of the 20 detected individual phenolic compounds were significantly enhanced by melatonin treatment in wine. Particularly, the content of two non-flavonoid phenolic compounds(syringic and coumaric acid) and four anthocyanin compounds [Mv-3-Glu, Mv-3-(6-Coum)Glu-5-Glu, Dp-3-(6-AC)Glu and Dp-3-(6-Coum)Glu-5-Glu]were largely increased by melatonin treatment. In contrast, only Pn-3-(6-Coum)Glu was significantly reduced. Moreover, melatonin treatment significantly enhanced the antioxidant capacity of wine indicated by DPPH and FRAP assays. In summary, melatonin treatment enhanced the polyphenol content and antioxidant capacity of wine.

关键词： grape red wine melatonin phenolics antioxidant capacity

RNA-sequencing analysis reveals novel genes involved in the different peel color formation in eggplant

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Horticulture Research 2023年第10期10卷 123-135页

作者： Jing Li Senlin Jiang Guobin Yang Yanwei Xu Lujun Li Fengjuan Yang College of Horticulture Science and Engineering Shandong Agricultural University/State Key Laboratory of Crop BiologyTai’anShandong 271018China key laboratory of Biology and genetic Improvement of Horticultural Crops(Huanghuai Region) Ministry of Agriculture and Rural AffairsTai’anShandong 271018China shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency Shandong Agricultural UniversityTai’anShandong 271018China

Eggplant(Solanum melongena L.)is a highly nutritious ***,the molecular mechanism of color formation in eggplants was determined using six eggplant cultivars with different peel colors and two SmMYB113-overexpressing transgenic eggplants with a purple peel and *** differentially expressed genes(DEGs)were identified by RNA-sequencing analysis using the following criteria:log2(sample1/sample2)≥0.75 and q-value≤*** analytical strategies were used to identify genes related to the different peel color according to the peel color,flavonoids content,delphinidins/flavonoids ratio,and the content of ***,27 novel genes were identified to be related to the color difference among eggplant peels and 32 novel genes were identified to be related to anthocyanin biosynthesis and regulated by *** analysis revealed that SmCytb5,SmGST,SmMATE,SmASAT3,and SmF3′5’M were shared among both sets of novel *** expression assay in tobacco suggested that these five genes were not sufficient for inducing anthocyanin biosynthesis alone,but they play important roles in anthocyanin accumulation in eggplant *** one-hybrid,electrophoretic mobility shift assay and dual-luciferase assays indicated that the expression of the five genes could be directly activated by SmMYB113 ***,a regulatory model for the mechanism of color formation in eggplant was ***,the results of this study provide useful information that enhances our understanding of the molecular mechanism underlying the different color formation in eggplant.

关键词： delphi analysis mechanism

Potassium Channels: A Potential Therapeutic Target for Parkinson's Disease

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Neuroscience Bulletin 2018年第2期34卷 341-348页

作者： Xiaoyan Chen Bao Xue Jun Wang Haixia Liu Limin Shi Junxia Xie Collaborative Innovation Center for Brain Science Department of Physiology Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders Key Laboratory of Pathogenesis and Prevention of Neurological Disorders Medical College of Qingdao University

The pathogenesis of the second major neurodegenerative disorder, Parkinson’s disease(PD), is closely associated with the dysfunction of potassium(K~+ ) channels. Therefore, PD is also considered to be an ion channel disease or neuronal channelopathy. Mounting evidence has shown that K~+ channels play crucial roles in the regulations of neurotransmitter release, neuronal excitability, and cell volume. Inhibition of K~+ channels enhances the spontaneous firing frequency of nigral dopamine(DA)neurons, induces a transition from tonic firing to burst discharge, and promotes the release of DA in the ***, three K~+ channels have been identified to protect DA neurons and to improve the motor and non-motor symptoms in PD animal models: small conductance(SK)channels, A-type K~+ channels, and KV7/KCNQ *** this review, we summarize the physiological and pharmacological effects of the three K~+ channels. We also describe in detail the laboratory investigations regarding K~+ channels as a potential therapeutic target for PD.

关键词： Parkinson’s disease A-type K+ channels SK channels KV7/KCNQ channels Dopamine