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An Indirect ELISA of Classical Swine Fever Virus Based on Quadruple Antigenic Epitope Peptide Expressed in ***

Virologica Sinica 2010年第1期25卷 71-76页

作者： Guo-zhen LIN Fu-ying ZHENG Ji-zhang ZHOU Xiao-an CAO Xiao-wei GONG Guang-hua WANG Chang-qing QIU key laboratories of grazing animal diseases and animal virology Ministry of Agriculture Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046 China State key Laboratory of Veterinao Etiological Biology Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046 China

In this study,a synthesized quadruple antigenic epitope gene region of the classical swine fever virus (CSFV)E2 glycoprotein was expressed in *** to a obtain target *** target protein was used as a coating antigen to establish an indirect ELISA for specifically detecting anti-CSFV antibodies in serum samples from *** P/N cut-off value of this assay was 1.92 by receiver operating characteristic curve(ROC)analysis based on 30 negative sera and 80 positive *** test gave 97.5%sensitivity and 96.7%specificity compared with the indirect hemagglutination(IHA)*** inter-assay and intra-assay coefficients of variation (CVs)for 16 sera were both≤6.8%.No cross-reactivity between the coating antigen and anti-bovine viral diarrhoea virus(BVDV)antibodies was observed.

关键词： Antigenic epitope Bovine viral diarrhoea virus (BVDV) Classical swine fever virus (CSFV) Expression Indirect ELISA

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Mycoplasmas and Mycoplasmosis in Sheep and Goats

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animal Husbandry and Feed Science 2010年第3期2卷 28-31页

作者： CHU Yue-feng ZHAO Ping GAO Peng-cheng HE Ying LU Zhong-xin key Laboratory of grazing animal diseases of Ministry of Agriculture Key Laboratory of Animal Virology of Ministry of Agricultureand State Key Laboratory of Veterinary Etiological BiologyLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou 730046China

The known mycoplasmas isolated from goats and sheep were listed. The pathogenicity of these mycoplasmas and their effects during occurrence of mycoplasmosis were summarized.

关键词： Mycoplasmas Mycoplasmosis Contagious agalactia Contagious caprine pleuropneumonia

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Epidemiological Investigation of Porcine Contagious Pleuropneumonia in Some Areas of Anhui Province

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animal Husbandry and Feed Science 2009年第4期1卷 36-37页

作者： HE Ying ZHAO Ping CHU Yue-feng GAO Peng-cheng LU Zhong-xin State key Laboratory of Veterinary Etiological Biology Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture and Key Laboratoryof Animal Virology of Ministry of Agriculture Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou730046 China

By indirect hemagglutination test, the prevalence of porcine contagious pleuropneumonia was investigated in part of pig farms in Anhui Province. The results showed that the total positive rate of this area was 16.6%, ... 详细信息

关键词： Porcine contagious pleuropneumonia Epidemiologic survey Anhui Province

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Diverse viromes in polar regions: A retrospective study of metagenomic data from Antarctic animal feces and Arctic frozen soil in 2012–2014

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Virologica Sinica 2022年第6期37卷 883-893页

作者： Jun Wang Jian Xiao Zheng Zhu Siyuan Wang Lei Zhang Zhaojun Fan Yali Deng Zhihong Hu Fang Peng Shu Shen Fei Deng State key Laboratory of virology and National Virus Resource Center Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China Xinjiang key Laboratory of Biological Resources and Genetic Engineering College of Life Science and TechnologyXinjiang UniversityUrumqi830046China China Center for Type Culture Collection(CCTCC) College of Life SciencesWuhan UniversityWuhan430072China Natisonal Emerging Infectious diseases laboratories Boston UniversityBostonMA 02118United States Department of Biochemistry Boston University School of MedicineBostonMA 02118United States

Antarctica and the Arctic are the coldest places, containing a high diversity of microorganisms, including viruses,which are important components of polar ecosystems. However, owing to the difficulties in obtaining access to animal and environmental samples, the current knowledge of viromes in polar regions is still limited. To better understand polar viromes, this study performed a retrospective analysis using metagenomic sequencing data of animal feces from Antarctica and frozen soil from the Arctic collected during 2012–2014. The results reveal diverse communities of DNA and RNA viruses from at least 23 families from Antarctic animal feces and 16families from Arctic soils. Although the viral communities from Antarctica and the Arctic show a large diversity,they have genetic similarities with known viruses from different ecosystems and organisms with similar viral proteins. Phylogenetic analysis of Microviridae, Parvoviridae, and Larvidaviridae was further performed, and complete genomic sequences of two novel circular replication-associated protein(rep)-encoding single-stranded(CRESS) DNA viruses closely related to Circoviridae were identified. These results reveal the high diversity,complexity, and novelty of viral communities from polar regions, and suggested the genetic similarity and functional correlations of viromes between the Antarctica and Arctic. Variations in viral families in Arctic soils,Arctic freshwater, and Antarctic soils are discussed. These findings improve our understanding of polar viromes and suggest the importance of performing follow-up in-depth investigations of animal and environmental samples from Antarctica and the Arctic, which would reveal the substantial role of these viruses in the global viral community.

关键词： Arctic soil Antarctica animal feces Metagenomics Virus diversity

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