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BSA-seq and transcriptomic analyses identify Chinese cabbage NB-LRR-encoding genes conferring resistance to soft rot disease

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Horticultural Plant Journal 2025年第2期11卷 943-946页

作者： Yunjia Ge Kaiyun Zhao Ni Wang Han Song Xudong Wang Hao Liang Xiaomeng Zhang Yin Lu Yanhua Wang Wei Ma Jianjun Zhao Lisong Ma Mengyang Liu State key laboratory of North China Crop Improvement and Regulation Key Laboratory for Vegetable Germplasm Innovation and Utilization of HebeiMinistry of Education of China-Hebei Province Joint Innovation Center for Efficient Green Vegetable IndustryHebei International Joint Research Center of Vegetable Functional GenomicsCollege of HorticultureHebei Agricultural UniversityBaodingHebei 071000China

Pectobacterium carotovorum(Pc)is a necrotrophic bacterial pathogen that causes soft rot disease in Brassica rapa crops,including Chinese cabbage(*** ***),resulting in significant yield losses worldwide(Roh et al.,2010).Deployment of resistant Chinese cabbage varieties and mining novel resistance genes for breeding resistant varieties against soft rot are key approaches that are currently used to prevent soft rot infections and ensure high yield and quality in Chinese ***,the highly adapted pathogen can easily overcome resistance(R)gene-mediated defense ***,discovering novel resistance genes and understanding the molecular mechanisms underlying R-mediated resistance against soft rot are sustained strategies for Chinese cabbage breeding.

关键词： breeding crops resistance

A PHYB-PIF4-auxin module promotes tomato graft formation in response to elevated ambient temperature

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Horticultural Plant Journal 2025年第1期11卷 227-238页

作者： Xianmin Meng Feng Zhang Wencai Yang Qingmao Shang State key laboratory of vegetable Biobreeding Key Laboratory of Horticultural Crop Biology and Germplasm Innovation(Ministry of Agriculture)Institute of Vegetables and FlowersChinese Academy of Agriculture SciencesBeijing 100081China Beijing key laboratory of Growth and Developmental Regulation for Protected vegetable Crops Department of Vegetable ScienceCollege of HorticultureChina Agricultural UniversityBeijing 100193China

Grafting is an effective technique for increasing the resistance of vegetables to biotic and abiotic *** has been widely applied to produce solanaceous and melon *** is an important external factor affecting graft ***,the molecular mechanism by which external ambient temperature affects tomato graft formation remains *** this study,we demonstrated that elevating ambient temperature during grafting to 35℃ for more than 24 h after grafting accelerated vascular *** generated self-or heterografted combinations between phyB1B2 and pif4 loss-of-function mutant and wild-type plants,and were mutants unresponsive to graft formation at elevated ambient *** addition,elevated ambient temperature induced SlPIF4 expression during ***4 directly binds the promoters of auxin biosynthesis genes SlYUCCAs and activates their *** investigation revealed auxin accumulation in the graft junction under elevated ambient *** results illuminate the mechanism by which the PHYB-PIF4-auxin module promotes tomato graft formation in response to elevated ambient temperature.

关键词： Graft formation Tomato Temperature Vascular Auxin

CBL1/CIPK23 phosphorylates tonoplast sugar transporter TST2 to enhance sugar accumulation in sweet orange(Citrus sinensis)

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Journal of Integrative Plant Biology 2025年第2期67卷 327-344页

作者： Mengdi Li Zuolin Mao Zeqi Zhao Siyang Gao Yanrou Luo Ziyan Liu Xiawei Sheng Xiawan Zhai Ji-Hong Liu Chunlong Li National key laboratory for germplasm innovation&utilization of Horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070China Hubei Hongshan laboratory Wuhan 430070China

Fruit taste quality is greatly influenced by the content of soluble sugars,which are predominantly stored in the vacuolar ***,the accumulation and regulation mechanisms of sugars in most fruits remain ***,we established the citrus fruit vacuole proteome and discovered the major transporters localized in the vacuole ***,we demonstrated that the expression of tonoplast sugar transporter 2(CsTST2)is closely associated with sugar accumulation during sweet orange(Citrus sinensis)*** was further demonstrated that CsTST2 had the function of transporting hexose and sucrose into the *** of CsTST2 resulted in an elevation of sugar content in citrus juice sac,calli,and tomato fruit,whereas the downregulation of its expression led to the reduction in sugar ***2 was identified as interacting with CsCIPK23,which binds to the upstream calcium signal sensor protein *** phosphorylation of the three serine residues(Ser277,Ser337,and Ser354)in the loop region of CsTST2 by CsCIPK23 is crucial for maintaining the sugar transport activity of ***,the expression of CsCIPK23 is positively correlated with sugar *** evidence further confirmed that calcium and CsCIPK23-mediated increase in sugar accumulation depends on CsTST2 and its phosphorylation *** findings not only unveil the functional mechanism of CsTST2 in sugar accumulation,but also explore a vital calcium signal regulation module of CsCBL1/CIPK23 for citrus sweetness quality.

关键词： citrus CsCBL1/CIPK23 phosphorylation tonoplast sugar transporter vacuole

Streamlined Agrobacterium rhizogenes-mediated hairy root transformation for efficient CRISPR/Cas9-based gene editing evaluation in diverse Citrullus cultivars

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Horticultural Plant Journal 2025年第2期11卷 816-826页

作者： Yong Zhao Hongju Zhu Xuqiang Lu Muhammad Anees Nan He Dongdong Yang Zihao Chen Zonglie Hong Junhong Zhang Wenge Liu National key laboratory for germplasm innovation&utilization of Horticultural Crops Zhengzhou Fruit Research InstituteChinese Academy of Agricultural SciencesZhengzhouHenan 450009China National key laboratory for germplasm innovation&utilization of Horticultural Crops Huazhong Agricultural UniversityWuhanHubei 430070China Department of Plant Sciences University of IdahoMoscowID 83844USA Zhongyuan Research Center Chinese Academy of Agricultural SciencesXinxiangHenan 453003China

The CRISPR/Cas9 genome-editing system serves as a pivotal tool for enhancing crop *** this system,single guide RNAs(sgRNAs)are instrumental in the precise cleavage of DNA double ***,the efficiency of gene editing varies among sgRNAs,emphasizing the need to meticulously select target sites,especially in the context of Citrullus lanatus,a species notorious for its challenging and inefficient generation of transgenic plants through stable *** study employed an Agrobacterium rhizogenes-mediated hairy root method to assess effective target sites for gene editing of ClCIPK17 across various Citrullus *** roots were successfully induced in different plant tissues at diverse growth stages of Citrullus lanatus,Citrullus mucosospermus,and Citrullus *** a vector with two sgRNAs(sgRNA1 and sgRNA5)positioned within conserved regions of exon 1 and exon 5 of ClCIPK17 in the CRISPR/Cas9 system,targeted mutations were detected in 90.9%of accessions across the four Citrullus ***,73.94%of all examined hairy roots exhibited mutations at the sgRNA1 site,while the sgRNA5 site showed no *** the 31 different mutation types identified at the sgRNA1 site,base deletion was the most *** the sgRNA1 site of ClCIPK17,stable transgenic watermelon buds were obtained from explants,and the targeted mutations of the sgRNA1 site were *** findings underscore the viability of the hairy root transformation system in assessing the editing efficiency of sgRNA targets in diverse Citrullus species.

关键词： Citrullus lanatus sgRNAs Agrobacterium rhizogenes Hairy root transformation CRISPR/Cas9

A novel C2H2-type zinc-finger transcription factor,CitZAT4,regulates ethylene-induced orange coloration in Satsuma mandarin flavedo(Citrus unshiu Marc.)

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Journal of Integrative Plant Biology 2025年第2期67卷 294-310页

作者： Quan Sun Zhengchen He Junli Ye Ranran Wei Di Feng Yingzi Zhang Lijun Chai Yunjiang Cheng Qiang Xu Xiuxin Deng National key laboratory for germplasm innovation and utilization of Horticultural Crops Huazhong Agricultural UniversityWuhan 430070China National Research Center for Apple Engineering and Technology Shandong Collaborative Innovation Center of Fruit and Vegetable Quality and Efﬁcient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTaian 271018China Hubei Hongshan laboratory Huazhong Agricultural UniversityWuhan 430070China

Ethylene treatment promotes orange coloration in the flavedo of Satsuma mandarin(Citrus unshiu Marc.)fruit,but the corresponding regulatory mechanism is still largely *** this study,we identified a C2H2-type zinc-finger transcription factor,CitZAT4,the expression of which was markedly induced by ***4 directly binds to the Cit PSY promoter and activates its expression,thereby promoting carotenoid *** expression in Satsuma mandarin fruit and stable transformation of citrus calli showed that overexpressing of CitZAT4 inhibited Cit LCYE expression,thus inhibitingα-branch yellow carotenoid(lutein)***4 overexpression also enhanced the transcript levels of CitLCYB,Cit HYD,and CitNCED2,promotingβ-branch orange carotenoid *** biochemical assays,including yeast one-hybrid(Y1H),electrophoretic mobility shift(EMSA),chromatin immunoprecipitation quantitative polymerase chain reaction(Ch IP-q PCR),and luciferase(LUC)assays,demonstrated that CitZAT4 directly binds to the promoters of its target genes and regulates their *** ethylene response factor,CitERF061,which is induced by ethylene signaling,was found to directly bound to the CitZAT4 promoter and induced its expression,thus positively regulating CitZAT4-mediated orange coloration in citrus ***,our findings reveal that a CitZAT4-mediated transcriptional cascade is driven by ethylene via CitERF061,linking ethylene signaling to carotenoid metabolism in promoting orange coloration in the flavedo of Satsuma mandarin *** molecular regulatory mechanism revealed here represents a significant step toward developing strategies for improving the quality and economic efficiency of citrus crops.

关键词： α-branch carotenoid β-branch carotenoid CitZAT4 ethylene orange coloration Satsuma mandarin

Emotional responses evoked by tree canopy landscape elements:a study based on two evaluation approaches

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Journal of Forestry Research 2025年第1期36卷 193-207页

作者： Wei Lin Tianyi Yao Chengcheng Zeng College of Landscape Architecture Zhejiang A&F UniversityHangzhou311300People’s Republic of China Zhejiang Provincial key laboratory of germplasm innovation and utilization for Garden Plants Zhejiang A&F UniversityHangzhou311300People’s Republic of China

Tree canopy landscapes are an important component of urban forests and have the potential to influence human ***,their influence on emotional responses remains *** aims of this study were:(1)to determine whether the canopy landscape affects human emotions;(2)to clarify the influence of canopy landscape on individual emotional indicators;and(3)to identify the ratio of canopy landscape elements with the most beneficial effects on human *** canopy landscape images were generated,and the self-reported emotions and neural activity of the subjects assessed before and after they viewed the *** results of the statistical analysis were intuitively displayed by a ternary phase *** found that the canopy landscape affected human emotions and different proportions of canopy landscape elements led to significant differences in excitement,depression and *** proportions of blue elements and lower proportions of green and other elements characterized the canopy landscape with the most beneficial effect on human *** findings will promote further research on canopy landscapes,inform the planning and design of urban forests,and contribute to the field of landscape architecture.

关键词： Emotional response Neural activity Canopy landscape

Transcriptome analysis reveals the common and specific pathways of citric acid accumulation in different citrus species

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Horticultural Plant Journal 2025年第2期11卷 520-534页

作者： Jiaxian He Juan Sun Yue Huang Lun Wang Shengjun Liu Zhenghua Jiang Xia Wang Qiang Xu National key laboratory for germplasm innovation&utilization of Horticultural Crops Huazhong Agricultural UniversityWuhanHubei430070China Hubei Hongshan laboratory WuhanHubei430070China

Citric acid is an important contributor to fruit *** extremely significant differences in citric acid levels are observed among citrus species and *** molecular basis of citric acid accumulation remains largely unknown on the inter-species ***,we performed transcriptome analysis of five pairs of acidic and acidless variety fruits from kumquat(Citrus crassifolia),lemon(***),pummelo(***),mandarin(***),and sweet orange(***)to investigate the common and specific genes related to citric acid accumulation in these citrus *** profiles of the citrate metabolism genes revealed that the mechanism of citric acid accumulation in kumquat differed from that in the other four *** comparative analysis and weighted gene co-expression network analysis identified 61 candidate genes from lemon,pummelo,mandarin,and sweet *** acidification regulated by CitPH4-CitAN1-CitPH5was a common mechanism underlying citric acid accumulation in these four *** addition,gene network analysis indicated that CitPH4 was a central regulator,exhibiting a strong correlation with the genes involved in vacuolar trafficking and potassium(K^(+))*** co-expression and functional enrichment analyses showed that active sugar metabolism and increased citrate synthesis were mainly responsible for the accumulation of citric acid in ***-four transcription factors were identified as candidate regulators,of which CitbHLH28 was found to regulate acidity level via transactivating CWINV2 and PK3 ***,this study provides an insight into the mechanism of citric acid accumulation in citrus fruit and provides useful references for fruit quality improvement through acidity control in different citrus species.

关键词： Citrus Citric acid Transcriptome Regulatory network Vacuolar acidiﬁcation Citrate synthesis

AvERF73 positively regulates waterlogging tolerance in kiwifruit by participating in hypoxia response and mevalonate pathway

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Horticultural Plant Journal 2025年第1期11卷 162-174页

作者： Danfeng Bai Yunpeng Zhong Shichao Gu Xiujuan Qi Leiming Sun Miaomiao Lin Ran Wang Yukuo Li Chungen Hu Jinbao Fang National key laboratory for germplasm innovation&utilization of Horticultural Crop Zhengzhou Fruit Research InstituteChinese Academy of Agricultural SciencesZhengzhouHenan 450009China key laboratory of Horticultural Plant Biology College of Horticulture&Forestry Sciences of Huazhong Agricultural UniversityWuhanHubei 430070China

Waterlogging stress is one of the greatest environmental threats to kiwifruit growth and ***-VII proteins have been demonstrated to play pivotal roles in regulating plant tolerance to ***,the genome-wide role of ERF-VII in kiwifruit waterlogging stress tolerance remains ***,we report the function and regulatory network of an ERF-VII transcription factor located to the nucleus,Av ERF73,in kiwifruit waterlogging *** of Av ERF73 in Arabidopsis thaliana and *** *** enhanced waterlogging tolerance in transgenic ***,we performed transcriptome analysis(RNA-seq)and DNA affinity purification sequencing(DAP-seq)to explore the regulatory mechanism of Av ***-seq coupled with DAP-seq showed that Av ERF73 might directly activate Ac NAC022 involved in the“cellular response to hypoxia”process and Ac HMGS1 involved in the mevalonate pathway to respond to waterlogging,which were also confirmed by a dual-luciferase reporter *** on our results,we propose a putative working model for controlling waterlogging tolerance by Av ERF73 in kiwifruit.

关键词： Actinidia ERF-VII Waterlogging stress RNA-seq DAP-seq

The critical role of CmCIPK1-CmRbohD1/D2 complexes in generating H_(2)O_(2) signals for enhancing salt tolerance in pumpkin

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Horticultural Plant Journal 2025年第2期11卷 804-815页

作者： Lanxing Wei Dandi Xu Lijian Zhou Hui Chen Zhaowen Peng Guoyu Chen Lihui Wang Haishun Cao Yuquan Peng Shouyu Geng Zhilong Bie National key laboratory for germplasm innovation&utilization of Horticultural Crops/College of Horticulture and Forestry Sciences Huazhong Agricultural UniversityWuhanHubei 430070China Hubei Hongshan laboratory WuhanHubei 430070China Institute of Facility Agriculture Guangdong Academy of Agricultural SciencesGuangzhouGuangdong 510640China

Pumpkin is of great economic importance not only as food resources but also as the most widely used rootstock to graft cucurbit *** rootstock improves salt tolerance of cucumber scion through respiratory burst oxidase homolog protein D(CmRbohD)-mediated reactive oxygen species(ROS)burst,which further enhances Na^(+)export and K^(+)*** D activation requires calcium ***,the underlying mechanism remains largely *** this study,we discovered two Rboh D members from pumpkin involved in the ROS burst at the early stage of salt ***1 and CmRbohD2 were functionally redundant and double mutation significantly impaired salt tolerance in *** of CmRbohD1 and CmRbohD2 mitigated salinity-induced damage and maintained a relatively low Na+content and high K+*** screened the potential calcineurin B-like interacting protein kinases(Cm CIPKs)which could bind with CmRbohD1 and *** results revealed that two Rboh Ds formed complexes specifically with CmCIPK1,thereby bursting ROS *** of CmCIPK1 promoted the early ROS burst under salt stress condition and maintained a relatively balanced Na+/K+***,we proposed a CmCIPK1-CmRbohD1/D2 complex for pumpkin salt stress signaling transduction,which regulates the ROS burst and Na+/K+*** findings offer unprecedented insights into the general mechanism of pumpkin salt tolerance.

关键词： Pumpkin Rboh Root transformation Salinity Protein interaction