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Transcript profiles of mitochondrial and cytoplasmic manganese superoxide dismutases in Exopalaemon carinicauda under ammonia stress

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Chinese Journal of Oceanology and Limnology 2015年第3期33卷 714-724页

作者：任海李健李吉涛刘萍梁忠秀吴建华 Hebei Normal University of Science and Technology key laboratory of sustainable development of marine fisheries Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences

Superoxide dismutase （SOD） is one of the most important antioxidant defense enzymes, and is considered as the first line against oxidative stress. In this study, we cloned a mitochondrial manganese （Mn） SOD （mMnSOD） cDNA from the ridgetail white prawn Exopalaemon carinicauda by using rapid amplification of cDNA ends （RACE） methods. The fulMength cDNA for mMnSOD was 1 014-bp long, containing a 5＇-untranslated region （UTR） of 37-bp, a 3＇-UTR of 321-bp with a poly （A） tail, and included a 657-bp open reading frame encoding a protein of 218 amino acids with a 16-amino-acid signal peptide. The protein had a calculated molecular weight of 23.87 kDa and a theoretical isoelectric point of 6.75. The mMnSOD sequence included two putative N-glycosylation sites （NHT and NLS）, the MnSOD signature sequence 18~DVWEHAYY^87, and four putative Mn binding sites （H48, H96, D180, and H184）. Sequence comparison showed that the mMnSOD deduced amino acid sequence of E. carinicauda shared 97%, 95%, 89%, 84%, 82%, 72%, and 69% identity with that ofMacrobrachium rosenbergii, Macrobrachium nipponense, Fenneropeneaus chinensis, Callinectes sapidus, Perisesarma bidens, Danio rerio, and Homo sapiens, resectively. Quantitative real-time RT-PCR analysis showed that mMnSOD transcripts were present in all E. carinicauda tissues examined, with the highest levels in the hepatopancreas. During an ammonia stress treatment, the transcript levels of mMnSOD and cMnSOD were up-regulated at 12 h in hemocytes and at 24 h in the hepatopancreas. As the duration of the ammonia stress treatment extended to 72 h, the transcript levels of mMnSOD and cMnSOD significantly decreased both in hemocytes and hepatopancreas. These findings indicate that the SOD system is induced to respond to acute ammonia stress, and may be involved in environmental stress responses in E. carinicauda.

关键词： Exopalaemon carinicauda mitochondrial manganese superoxide dismutase (mMnSOD) cytosolic manganese superoxide dismutase (cMnSOD) cloning expression

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Effects of acute salinity stress on the survival and prophenoloxidase system of Exopalaemon carinicauda

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Acta Oceanologica Sinica 2020年第4期39卷 57-64页

作者： Qianqian Ge Zhengdao Li Jitao Li Jiajia Wang Jian Li key laboratory of sustainable development of marine fisheries Ministry of Agriculture/Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China laboratory for marine fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

The ridgetail white prawn Exopalaemon carinicauda is a euryhaline shrimp species in the estuarine and coastal areas of *** this study,survival rates,transcription levels of two prophenoloxidase system-related genes(Ec LGBP and Ecpro PO)and PO activity were determined quantitatively in juvenile and adult *** under different salinity *** results showed that *** juveniles could survive in a wider range of salinity conditions than *** juvenile ***,the expression levels of Ec LGBP and Ec Pro PO were upregulated in low salinities and showed no significant difference at 20–40,while PO activities in low salinities were higher compared to those in high *** adult ***,the expression profiles of Ec LGBP and Ecpro PO had a different trend of up-regulation in salinity stress treatments and no obvious difference was observed in the gene expression levels and PO activity between 30 and *** salinity tolerance range of immunity for juvenile and adult *** is 20–40 and 30–40,respectively.

关键词： Exopalaemon carinicauda prophenoloxidase system salinity stress immunity tolerant range

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Impact of Vibrio parahaemolyticus and white spot syndrome virus(WSSV)co-infection on survival of penaeid shrimp Litopenaeus vannamei

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Chinese Journal of Oceanology and Limnology 2016年第6期34卷 1278-1286页

作者：张晓静宋晓玲黄倢 key laboratory for sustainable development of marine fisheries Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery ScienceQingdao 266071China Qingdao National laboratory for marine Science and Technology Qingdao 266200China

White spot syndrome virus(WSSV) is an important viral pathogen that infects farmed penaeid shrimp, and the threat of Vibrio parahaemolyticus infection to shrimp farming has become increasingly severe. Viral and bacterial cross or superimposed infections may induce higher shrimp mortality. We used a feeding method to infect L itopenaeus vannamei with WSSV and then injected a low dose of V. parahaemolyticus(WSSV+Vp), or we fi rst infected L. vannamei with a low-dose injection of V. parahaemolyticus and then fed the shrimp WSSV to achieve viral infection(Vp+WSSV). The effect of V. parahaemolyticus and WSSV co-infection on survival of L. vannamei was evaluated by comparing cumulative mortality rates between experimental and control groups. We also spread L. vannamei hemolymph on thiosulfate citrate bile salt sucrose agar plates to determine the number of V ibrio, and the WSSV copy number in L. vannamei gills was determined using an absolute quantitative polymerase chain reaction(PCR) method. L v My D88 and Lvakt gene expression levels were detected in gills of L. vannamei by real-time PCR to determine the cause of the different mortality rates. Our results show that(1) the cumulative mortality rate of L. vannamei in the WSSV+Vp group reached 100% on day 10 after WSSV infection, whereas the cumulative mortality rate of L. vannamei in the Vp+WSSV group and the WSSV-alone control group approached 100% on days 11 and 13 of infection;(2) the number of Vibrio in the L. vannamei group infected with V. parahaemolyticus alone declined gradually, whereas the other groups showed signifi cant increases in the numbers of Vibrio( P <0.05);(3) the WSSV copy numbers in the gills of the WSSV+Vp, Vp+WSSV, and the WSSV-alone groups increased from 10 5 to 10 7 /mg tissue 72, 96, and 144 h after infection, respectively. These results suggest that V. parahaemolyticus infection accelerated proliferation of WSSV in L. vannamei and vice versa. The combined accelerated proliferation of both V. p

关键词： Vibrio parahaemolyticus Litopenaeus vannamei white spot syndrome virus(WSSV) coinfection

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Inbreeding and genetic diversity analysis in a hatchery release population and clones of Rhopilema esculentum based on microsatellite markers

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Chinese Journal of Oceanology and Limnology 2017年第3期35卷 580-586页

作者：田涛陈再忠王陌桑胡玉龙王伟继 College of fisheries and Life Science Shanghai Ocean University key laboratory of sustainable development of marine fisheries Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Function laboratory for marine fisheries Science and Food Production Processes National Laboratory for Ocean Science and Technology

Ten microsatellite markers were used to analyze the levels of genetic diversity and inbreeding in a hatchery release population of Rhopilema esculentum Kishinouye （Scypbozoa： Rhizostomatidae）. A total of 85 alleles were detected in 600 individuals. Within-population levels of observed （Ho） and expected （He） heterozygosity ranged from 0.152 to 0.839 （mean=0.464） and from 0.235 to 0.821 （mean=0.618）, respectively. The polymorphism information content （PIC） of each marker ranged from 0.207 to 0.795 with an average of 0.580, indicating that the hatchery population maintained a high level of genetic diversity. Inbreeding levels were estimated in the hatchery population and the inbreeding coefficient was 0.203. This result revealed that a certain level of inbreeding occurred within the population. Meanwhile, we also determined genetic diversity at the clone level. Several polyps from the same scyphistomae were genotyped at the ten microsatellite loci and there was virtually no difference in their genotypes. Furthermore, we calculated the probabilities of exclusion. When both parents were known, the average exclusion probability often loci was 99.99%. Our data suggest that the ten microsatellite markers can not only be used to analyze the identity of individuals but they can also be applied to parentage identification. Our research provides a theoretical basis and technical support for genetic diversity detection and reasonable selection of R. esculentum hatchery populations. These findings support the use of releasing studies and conservation of R. esculentum germplasm resources.

关键词： Rhopilema esculentum hatchery release genetic diversity inbreeding microsatellite markers clones

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Heritability and genetic correlation of survival in turbot(Scophthalmus maximus)

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Chinese Journal of Oceanology and Limnology 2010年第6期28卷 1200-1205页

作者：王新安马爱军黄智慧周洲 Yellow Sea fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao key laboratory for marine Fish Breeding and Biotechnology key laboratory for sustainable Utilization of marine fisheries Resources Ministry of Agriculture

We analyzed the survival data of the offspring from 21 sires and 42 dams of turbot. The results show that the cumulative survival rates for turbot from 2 to 18 months range from 17.5% to 28.5% main mortality occurred during months 2-5; and the highest survival rates of families were 97.9%, 98.8%, 99.4%, 99.7% during months 2-5, 5-6, 6-8, 8-11, and ll-18, respectively, and 99.5%, being 53.5%, 23.8%, 19.5%, 14.9%, and 13.2% higher, respectively, than the mean values in each period. In all periods, the estimated heritabilities for survival were very low without significant difference from zero （P〉0.05）（values ranged from 0.06 to 0.12）, indicating low additive genetic effects. The genetic correlations of survival among families in different periods were all positive, but low in magnitude （values range from 0.03 to 0.31）. Genetic correlations between long-term survival and other periods＇ survival had negative values （-0.06 and -0.15） and three positive values （0.16, 0.12 and 0.14）. Genetic correlations between survival and weight were all positive, except for survival at months 2-5 and weight at 18 months, which was not significantly negative （-0.18）.

关键词： Scophthalmus maximus L. survival heritability genetic correlation

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Functional morphology of the olfactory organ of the tongue sole, Cynoglossus semilaevis

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Chinese Journal of Oceanology and Limnology 2010年第2期28卷 209-217页

作者：马爱军王新安 Yellow Sea fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao key laboratory for marine Fish Breeding and Biotechnology key laboratory for sustainable Utilization of marine fisheries Resources Ministry of Agriculture

The morphology and structure of the olfactory organ of Cynoglossus semilaevis Gunther are described. The oval olfactory sacs on both sides differ in size and in the number of lamellae, With those on the abocular side having smaller sacs and fewer lamellae than those on the ocular side. On the ocular side, the average ratio of sac length to eye diameter is 2.1 （i.e.〉1） with an average of 91 lamellae, while on the abocular side, the values were 1.7 （i.e.〉1） and 69, respectively. In addition, the surface morphology varies in different parts of the lamella. The frontal part, near the anterior nostril, is a non-sensory margin with cilia-free epidermal cells. Within this is an internal ciliated sensory area, which is intercalated with ciliated receptor cells and a few ciliated non-sensory cells. Additionally, some dense ciliated non-sensory cells make up a non-sensory area, which also contains cilia-free epidermal cells distributed in patches. In the rear of the olfactory sac near the posterior nostril, the lamellae differ in morphology from those of the frontal olfactory sac but are similar in having few ciliated receptor cells. In other words, the surface of the lamellae in the rear part of the olfactory sac is mainly non-sensory. At present, four types of lamellae （~ E IlIand IV） have been recognized in relation to the pattern of the sensory epithelium. In this study, the frontal and rear lamellae resembled types I and IV, respectively, but are referred to as types r and IV because they are slightly less developed. Data on the ratio of length of lamellae to eye diameter, number of lamellae and the type of surface pattern of the lamellae show that the development of the olfactory system of C. semilaevis facilitates prey capture.

关键词： Cynoglossus semilaevis tongue sole olfactory organ morphology

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Carbon and nitrogen isotopes analysis and sources of organic matter in surface sediments from the Sanggou Bay and its adjacent areas, China

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Acta Oceanologica Sinica 2014年第12期33卷 48-57页

作者： XIA Bin CUI Yi CHEN Bijuan CUI Zhengguo QU Keming MA Feifei key laboratory of sustainable Utilization of marine fisheries Resources of Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fisheries Sciences

Naturally existing stable carbon and nitrogen isotopes are important in the study of sedimentary organic matter sources. To identify the sources of sedimentary organic matter in Sanggou Bay and its adjacent areas, which is characterized by high-density shellfish and seaweed aquaculture, the grain size, organic carbon （OC）, total nitrogen （TN）, carbon and nitrogen isotopic composition （δ13C andδ15N） of organic matter in the surface sediment were determined. The results showed that, in August, sedimentary OC and TN ranged from 0.17% to 0.76% and 0.04% to 0.14%, respectively. In November, OC and TN ranged from 0.23% to 0.87% and 0.05% to 0.14%, respectively. There was a significant positive correlation between OC and TN （R=0.98, P＆lt;0.0001）, indicating that OC and TN were homologous. In August, theδ13C andδ15N of organic matter varied from -23.06‰ to -21.59‰ and 5.10‰ to 6.31‰, respectively. In November,δ13C andδ15N ranged from -22.87‰ to -21.34‰ and 5.13‰ to 7.31‰, respectively. This study found that the major sources of sedimentary organic matter were marine shellfish biodeposition, seaweed farming, and soil organic matter. Using a three-end-member mixed model, we estimated that the dominant source of sedimentary organic matter was shellfish biodeposition, with an average contribution rate of 65.53% in August and 43.00% in November. Thus, shellfish farming had a significant influence on the coastal carbon cycle.

关键词： carbon nitrogen stable isotopes organic matter sources sediment Sanggou Bay

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Decadal variations in the community status of economically important invertebrates in the Bohai Sea

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Acta Oceanologica Sinica 2019年第10期38卷 60-66页

作者： Qiang Wu Lisha Guan Zhongyi Li Xiujuan Shan Xianshi Jin key laboratory for sustainable development of marine fisheries Ministry of Agriculture/Shandong Provincial Key Laboratory for Fishery Resources and Eco-environmentYellow Sea Fisheries Research InstituteAcademy of Fishery SciencesQingdao 266071China laboratory for marine fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

With the decline of fish stocks,the proportions of economically important invertebrates like crustaceans and cephalopods have increased in the Bohai *** community structure and trophic level of economically important invertebrates were analyzed using the bottom trawl survey data collected by the Yellow Sea fisheries Research Institute in the Bohai Sea in May and August of 1958–1959,1982,1992–1993,2004,2009 and 2015.A total of 37 species of economically important invertebrates,belonging to 5 orders,24 families,were *** biomass densities of economically important invertebrates in the Bohai Sea displayed an overall downward trend from 1982 to *** oratoria and Loligo *** the most dominant species in the past 30 years,the biomass proportion of *** increased gradually in both May and August from 1982 to ***,biodiversity indices of economically important invertebrates in the Bohai Sea appeared to decline from 1982 to 2004 and then increased in ***,the mean trophic level of economically important invertebrates declined from 1982 to 2004 and increased slightly in ***,although the proportions of invertebrates have increased,the biomass densities in the Bohai Sea have displayed an overall downward trend from 1982 to *** increases in the biodiversity and trophic level of economically important invertebrates after the 2000s,possibly benefit from stock enhancement projects implemented by governments at different levels and national fishery management measures such as the“double-control”of the total number and engine power of fishing vessels and summer moratorium of fishing.

关键词： decadal variations community structure trophic level economically important invertebrates BohaiSea

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Phylogenetic diversity of culturable bacteria in surface seawater from the Drake Passage,Antarctica

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Chinese Journal of Oceanology and Limnology 2016年第5期34卷 952-963页

作者：李昭邢孟欣王伟王丹朱建成孙谧 Yellow Sea fisheries Research Institute Chinese Academy of Fishery Sciences Key Laboratory of Sustainable Development of Marine FisheriesMinistry of AgricultureResearch Center for Quality and Safety of Marine Biological Resources and Control Engineering in Shandong

The Drake Passage is located between the Antarctic Peninsula and Tierra del Fuego in the south of South *** seawater samples were collected at seven sites in the Drake Passage during the austral summer of *** 16 S rRNA sequences were analyzed from 187 isolated bacterial *** phyla,29 genera and 56 species were *** three phyla were Actinobacteria,Firmicutes and Proteobacteria;the Proteobacteria included a-Proteobacteria,P-Proteobacteria and y-Proteobacteria.y-Proteobacteria,Actinobacteria and Firmicutes were the dominant class or phyla in terms of quantity and ***-positive bacteria(Actinobacteria and Firmicutes) accounted for 57.8% of all types *** were nine dominant genera,including Curtobacterium,Staphylococcus,and Halomonas,and 14 dominant species including Curtobacterium flaccumfaciens,Curtobacterium pusillum,and Staphylococcus *** the strains identified,87.2% were catalase positive or weakly positive.

关键词： Antarctic surface seawater culturable bacteria phylogenetic diversity catalase activities

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Molecular Characterization and Expression and DNA Methylation Analyses of a Galectin-Related Protein Gene from Cynoglossus semilaevis

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Journal of Ocean University of China 2018年第5期17卷 1189-1196页

作者： XIU Yunji GAN Tian XU Wenteng SHAO Changwei CHEN Songlin laboratory for marine fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyYellow Sea Fisheries Research InstituteChinese Academy of Fishery Sciences key laboratory of sustainable development of marine fisheries Ministry of Agriculture marine Science and Engineering College Qingdao Agricultural University College of fisheries and Life Science Shanghai Ocean University

Galectins, a family of β-galactoside-binding proteins, participate in both innate immunity and adaptive immunity. This study identified one novel galectin-related protein from half-smooth tongue sole Cynoglossus semilaevis, which was designated as CsGRP. The full-length cDNA of its encoding gene was 785 bp in length with a 528 bp open reading frame encoding a putative protein of 176 amino acids. The deduced CsGRP contained a putative 131-aa galactoside-binding lectin domain and 3 critical residues responsible for carbohydrate binding （R93, W109 and R114）. Genomic structural analysis revealed that CsGRP consisted of five exons and four intzons. CsGRP showed 68% similarity with Poecilia latipinna GRP and 67% similarity wih Stegastes partitus GRP. CsGRP showed the highest expression level in liver, although its expression was detected in all tested tissues. When challenged with Vibrio harveyi, the expression of CsGRP was significantly down-regulated in liver （P〈 0.05）. In addition, we found that in spleen and kidney of C. semilaevis, the CpG island of CsGRP showed significantly higher （P 〈 0.05） methylation level in disease-resistant family of C. semilaevis （DR-Cs） than in disease-susceptible ones （DS-Cs）. Our results suggested that CsGRP may play important roles in the immune response of C. semilaevis. Moreover, DNA methylation patterns provided valuable data for understanding the relationship between epigenetic regulation and immunity, which would assist the animal genetic research and improve the animal breeding in the future.

关键词： Cynoglossus semilaevis galectin-related protein expression pattern DNA methylation immune response

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