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Chinese Journal of Oceanology and Limnology 2013年第1期31卷 81-91页

作者：刘必林陈新军陈勇田思泉李建华方舟杨铭霞 College of marine Sciences Shanghai Ocean University The key laboratory of sustainable Exploitation of Oceanic fisheries Resources Ministry of Education School of marine Sciences University of Maine Orono

Age, maturation and population structure of the Humboldt squid Dosidicus gigas were studied based on random sampling of the Chinese jigging fishery off the Peruvian Exclusive Economic Zones （EEZ） during 2008-2010. Estimated ages ranged from 144 to 633 days, confirming that the squid is a short-lived species with longevity no longer than 2 years. Occurrence of mature females and hatching in each month indicated that Humboldt squid spawned year-round. Back-calculated hatching dates for the samples were from January 22^nd, 2008 to April 22nd, 2010 with a peak between January and March. Two size-based and two hatching date-based populations could be defined from mantle length （ML） at maturity and back-calculated hatching dates, respectively. Females matured at a larger size than males, and there was a significant difference in ML at maturity between the two hatching groups （P〈0.05）. The waters adjacent to 1 l^S off the Peruvian EEZ may be a potential spawning ground. This study shows the complexity of the population structure and large variability in key life history parameters in the Humboldt squid off the Peruvian EEZ, which should be considered in the assessment and management of this important resource.

关键词： Dosidicus gigas offPeruvian EEZ age maturation population structure Statolith

Molecular characterization and expression analysis of Lily- type lectin (SmLTL) in turbot Scophthalmus maximus, and its response to Vibrio anguillarum

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Journal of Oceanology and Limnology 2018年第2期36卷 508-518页

作者： XIA Dandan MA Aijun HUANG Zhihui SHANG Xiaomei CUI Wenxiao YANG Zhi QU Jiangbo key laboratory of sustainable development of marine fisheries Ministry of Agriculture Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 China Ocean University of Shanghai Shanghai 201306 China laboratory for marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China Yantai Tianyuan Aquatic Limited Corporation Yantai 264006 China

A full-length lily-type lectin （SmLTL） was identified from turbot （Scophthalmus maximus） in this study. By searching database for protein identification and fimction prediction, SmLTL were confirmed. The full-length cDNA of SmLTL is composed of 569 bp and contains a 339 bp ORF that encodes 112 amino acid residues. The SmLTL peptide is characterized by a specific J3-prism architecture and contains three mannose binding sites in a three-fold internal repeat between amino acids 30-99; two of the repeats share the classical mannose binding domain （QxDxNxVxY） while the third binding site was similar to other fish-specific binding motifs （TxTxGxRxV）. The primary, secondary, and tertiary structures of SmLTL were predicted and analyzed, indicating that the SmLTL protein was hydrophilic, contained 5.36% a-helices, 39.29% extended strands, 16.07% [3-folds, and 39.29% random coils, and three [3-folds. Quantitative real- time polymerase chain reaction （qPCR） analysis revealed that the SmLTL mRNA was abundantly expressed in skin, gill, and intestine. Low levels of SmLTL expression were observed in other tissues. The expression of SmLTL in gill, skin and intestine increased at mRNA level after stimulation of Hbrio anguillarum, our results suggest that SmLTL serve as the first line of defence against microbial infections and play a pivotal role in the innate mucosal immune system. The current study indicates that SmLTL is a member of the lily- type lectin family and the information reported here will provide an important foundation for future research on the role of this protein.

关键词： lily-type lectin full-length of cDNA quantitative real-time polymerase chain reaction protein structure Vibrio anguillarum

Genomics and genetic breeding in aquatic animals:progress and prospects

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Frontiers of Agricultural Science and Engineering 2017年第3期4卷 305-318页

作者： Wenteng XU Songlin CHEN key Lab for sustainable development of marine fisheries Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery Sciences laboratory for marine fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and Technology

Genomics focuses on dissection of genome structure and function to provide a molecular basis for understanding the genetic background. In a pivotal step,the expense of whole genome sequencing has been largely eliminated by the rapid updating of sequencing technology,leading to increasing numbers of decoded genomes of aquatic organisms, driving the aquaculture industry into the genomic era. Multiple aquatic areas have been influenced by these findings, such as accelerated generation shift in the seed industry and the process of breeding improved lines. In this article, we have summarized the latest domestic and international progress of aquatic animals in nine aspects, including WGS and fine mapping,construction of high density genetic/physical maps, traitrelated marker/genes screening, as well as sex control,genome editing, and other molecular breeding technologies. Finally, the existing problems in this field have been discussed and five future counter measures have been proposed accordingly.

关键词： aquaculture genomics genetic breeding progress

Management strategies of marine food resources under multiple stressors with particular reference of the Yellow Sea large marine ecosystem

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Frontiers of Agricultural Science and Engineering 2014年第1期1卷 78-83页

作者： Qisheng TANG key laboratory of sustainable development of marine fisheries Ministry of AgricultureCarbon Sink Fisheries LaboratoryYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China

In this study two main management strategies are discussed:one is to develop resource conservationbased capture fisheries,and the other is to develop environmentally friendly *** the resource recovery period,the development of environmentally friendly aquaculture should be encouraged,especially in integrated multi-trophic aquaculture,which is adaptive,efficient and *** future development and better understanding the ecosystem,it is necessary to further strengthen basic research.

关键词： marine ecosystem management strategies food resources Yellow Sea

Modeling Monthly Spatial Distribution of Ommastrephes bartramii CPUE in the Northwest Pacific and Its Spatially Nonstationary Relationships with the marine Environment

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Journal of Ocean University of China 2018年第3期17卷 647-658页

作者： FENG Yongjiu LIU Yang CHEN Xinjun College of marine Sciences Shanghai Ocean University Function laboratory for marine fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and Technology key laboratory of sustainable Exploitation of Oceanic fisheries Resources Shanghai Ocean University National Distant-Water fisheries Engineering Research Center Shanghai Ocean University

There are substantial spatial variations in the relationships between catch-per-unit-effort(CPUE) and oceanographic conditions with respect to pelagic species. This study examines the monthly spatiotemporal distribution of CPUE of the neon flying squid, Ommastrephes bartramii, in the Northwest Pacific from July to November during 2004–2013, and analyzes the relationships with oceanographic conditions using a generalized additive model(GAM) and geographically weighted regression(GWR) model. The results show that most of the squids were harvested in waters with sea surface temperature(SST) between 7.6 and 24.6℃, chlorophyll-a(Chl-a) concentration below 1.0 mgm^(-3), sea surface salinity(SSS) between 32.7 and 34.6, and sea surface height(SSH) between-12.8 and 28.4 cm. The monthly spatial distribution patterns of O. bartramii predicted using GAM and GWR models are similar to observed patterns for all months. There are notable variations in the local coefficients of GWR, indicating the presence of spatial non-stationarity in the relationship between O. bartramii CPUE and oceanographic conditions. The statistical results show that there were nearly equal positive and negative coefficients for Chl-a, more positive than negative coefficients for SST, and more negative than positive coefficients for SSS and SSH. The overall accuracies of the hot spots predicted by GWR exceed 60%(except for October), indicating a good performance of this model and its improvement over GAM. Our study provides a better understanding of the ecological dynamics of O. bartramii CPUE and makes it possible to use GWR to study the spatially nonstationary characteristics of other pelagic species.

关键词： Ommastrephes bartramii catch-per-unit-effort (CPUE) oceanographic conditions geographically weighted regression (GWR) spatial nonstationary Northwest Pacific

Complete genome of Cobetia marina JCM 21022T and phylogenomic analysis of the family Halomonadaceae

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Journal of Oceanology and Limnology 2018年第2期36卷 528-536页

作者： TANG Xianghai XU Kuipeng HAN Xiaojuan MO Zhaolan MAO Yunxiang Ministry of Education key laboratory of marine Genetics and Breeding College of Marine Life Sciences Ocean University of China Qingdao 266003 China key laboratory of sustainable development of marine fisheries Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 China CAS key laboratory of Biobased Materials Qingdao Institute of Bioenergy and Bioproeess Technology Chinese Academy of Sciences Qingdao 266101 China

Cobetia marina is a model proteobacteria in researches on marine biofouling. Its taxonomic nomenclature has been revised many times over the past few decades. To better understand the role of the surface-associated lifestyle of C. marina and the phylogeny of the family Halomonadaceae, we sequenced the entire genome of C. marina JCM 21022T using single molecule real-time sequencing technology （SMRT） and performed comparative genomics and phylogenomics analyses. The circular chromosome was 4 176 300 bp with an average GC content of 62.44% and contained 3 611 predicted coding sequences, 72 tRNA genes, and 21 rRNA genes. The C. marina JCM 2102U genome contained a set of crucial genes involved in surface colonization processes. The comparative genome analysis indicated the significant differences between C. marina JCM 21022T and Cobetia amphilecti KMM 296 （formerly named C. marina KMM 296） resulted from sequence insertions or deletions and chromosomal recombination. Despite these differences, pan and core genome analysis showed similar gene functions between the two strains. The phylogenomic study of the family Halomonadaceae is relationships were well resolved among every genera Cobetia, Kushneria, Zymobacter, and Halotalea. reported here for the first time. We found that the tested, including Chromohalobacter, Halomonas,

关键词： Cobetia marina JCM 21022r Halomonadaceae complete genome sequence comparativegenomics phylogenomics surface colonization single molecule real-time sequencingtechnology (SMRT)

Impacts of changing scale on Getis-Ord Gi* hotspots of CPUE:a case study of the neon flying squid (Ommastrephes bartramii)in the northwest Pacific Ocean

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Acta Oceanologica Sinica 2018年第5期37卷 67-76页

作者： FENG Yongjiu CHEN Xinjun GAO Feng LIU Yang College of marine Sciences Shanghai Ocean University laboratory for marine fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and Technology National Distant-water fisheries Engineering Research Center Shanghai Ocean University key laboratory of sustainable Exploitation of Oceanic fisheries Resources (Shanghai Ocean University) Ministry of Education

We examined the scale impacts on spatial hot and cold spots of CPUE for Ommastrephes bartramii in the northwest Pacific Ocean. The original fishery data were tessellated to 18 spatial scales from 5′×5′ to 90′×90′ with a scale interval of 5′ to identify the local clusters. The changes in location, boundaries, and statistics regarding the Getis-Ord Gi＊ hot and cold spots in response to the spatial scales were analyzed in detail. Several statistics including Min, mean, Max, SD, CV, skewness, kurtosis, first quartile（Q1）, median, third quartile（Q3）, area and centroid were calculated for spatial hot and cold spots. Scaling impacts were examined for the selected statistics using linear, logarithmic, exponential, power law and polynomial functions. Clear scaling relations were identified for Max, SD and kurtosis for both hot and cold spots. For the remaining statistics, either a difference of scale impacts was found between the two clusters, or no clear scaling relation was identified. Spatial scales coarser than 30′ are not recommended to identify the local spatial patterns of fisheries because the boundary and locations of hot and cold spots at a coarser scale are significantly different from those at the original scale.

关键词： Ommastrephes bartramii scale impacts local clusters Getis-Ord Gi* spatial hotspots

Application of a Bayesian method to data-poor stock assessment by using Indian Ocean albacore (Thunnus alalunga) stock assessment as an example

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Acta Oceanologica Sinica 2016年第2期35卷 117-125页

作者： GUAN Wenjiang TANG Lin ZHU Jiangfeng TIAN Siquan XU Liuxiong College of marine Sciences Shanghai Ocean University key laboratory of sustainable Exploitation of Oceanic fisheries Resources (Shanghai Ocean University) Ministry of Education

It is widely recognized that assessments of the status of data-poor fish stocks are challenging and that Bayesian analysis is one of the methods which can be used to improve the reliability of stock assessments in data-poor situations through borrowing strength from prior information deduced from species with good-quality data or other known information. Because there is considerable uncertainty remaining in the stock assessment of albacore tuna（Thunnus alalunga） in the Indian Ocean due to the limited and low-quality data, we investigate the advantages of a Bayesian method in data-poor stock assessment by using Indian Ocean albacore stock assessment as an example. Eight Bayesian biomass dynamics models with different prior assumptions and catch data series were developed to assess the stock. The results show（1） the rationality of choice of catch data series and assumption of parameters could be enhanced by analyzing the posterior distribution of the parameters;（2） the reliability of the stock assessment could be improved by using demographic methods to construct a prior for the intrinsic rate of increase（r）. Because we can make use of more information to improve the rationality of parameter estimation and the reliability of the stock assessment compared with traditional statistical methods by incorporating any available knowledge into the informative priors and analyzing the posterior distribution based on Bayesian framework in data-poor situations, we suggest that the Bayesian method should be an alternative method to be applied in data-poor species stock assessment, such as Indian Ocean albacore.

关键词： data-poor stock assessment Bayesian method catch data series demographic method Indian Ocean Thunnus alalunga

Abnormal Karyotype of Pen Shell (Atrina pectinata) During Its Early Embryonic development in Late Breeding Season

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Journal of Ocean University of China 2020年第4期19卷 902-910页

作者： ZHOU Liqing WANG Xuemei YANG Aiguo WU Biao SUN Xiujun LIU Zhihong CHEN Siqing ZHAO Dan key laboratory for sustainable development of marine fisheries of Ministry of Agriculture Yellow Sea Fisheries Research InstituteChinese Academy of Fishery ScienceQingdao 266071China laboratory for fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China Rizhao Ocean and fisheries Research Institute Rizhao 276826China College of fisheries and Life Science Shanghai Ocean UniversityShanghai 201306China

The pen shell,Atrina pectinata,distributes *** is one of the most important edible bivalves in east Asian ***,there are multiple difficulties in rearing pen shell larvae and juveniles because of their high *** understand the mechanism underlining such high mortality at the early embryonic development stage,we obtained approximately 100 million larvae during later breeding season in June,2017,and checked a large amount of mitotic chromosomal plates of the early embryos and post-spawning gonads tissue slices of their *** results showed that most diploid *** embryos have 17 pairs of chromosomes(2n=34)as their parents *** first pair of particularly large chromosomes are heterotypic in some diploid embryos while they are homomorphic in *** primary sex-determination chromosome type is XX/XY.A lot of triploid,pentaploid and aneuploid embryos with different numbers of the largest homomorphic or heteromorphic chromosomes were found due to the degeneration of overmatured parent gonads which hold normal *** larvae will die even though most of them may develop into the trochophore stage with 34 *** deficiency of chromosomes will cause a high rate of mortality in early embryos in late breeding *** findings should enrich the current knowledge of juvenile pen shell aquaculture.

关键词： Atrina pectinata early embryo sex chromosome nuclear ploidy later breeding season

Histo-blood group antigens in Crassostrea gigas and binding profiles with GⅡ.4 Norovirus

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Journal of Oceanology and Limnology 2018年第4期36卷 1383-1391页

作者： MA Liping LIU Hui SU Laij in ZHAO Feng ZHOU Deqing DUAN Delin key laboratory of Experimental marine Biology Institute of OceanologyChinese Academy of SciencesQingdao 266071China key laboratory for sustainable Utilization of marine fisheries Resources Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China laboratory for marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China

Noroviruses(NoVs) are the main cause of viral gastroenteritis outbreaks worldwide, and oysters are the most common carriers of NoV contamination and transmission. NoVs bind specifically to oyster tissues through histo-blood group antigens(HBGAs), and this facilitates virus accumulation and increases virus persistence in oysters. To investigate the interaction of HBGAs in Pacific oysters with GⅡ.4 NoV, we examined HBGAs with ELISAs and investigated binding patterns with oligosaccharide-binding assays using P particles as a model of five GⅡ.4 NoV capsids. The HBGAs in the gut and gills exhibited polymorphisms. In the gut, type A was detected(100%), whereas type Leb(91.67%) and type A(61.11%) were both observed in the gills. Moreover, we found that seasonal NoV gastroenteritis outbreaks were not significantly associated with the specific HBGAs detected in the oyster gut and gills. In the gut, we found that strain-2006 b and strain-96/96 US bound to type A and H1 but only weakly bound to type Leb; in contrast, the Camberwell and Hunter strains exhibited weak binding to types H1 and Ley, and strain-Sakai exhibited no binding to any HBGA type. In the gills, strain-96/96 US and strain-2006 b bound to type Leb but only weakly bound to type H1; strains Camberwell, Hunter, and Sakai did not bind to oyster HBGAs. Assays for oligosaccharide binding to GⅡ.4 NoV P particles showed that strain-95/96 US and strain-2006 b strongly bound to type A, B, H1, Leb, and Ley oligosaccharides, while strains Camberwell and Hunter showed weak binding ability to type H1 and Ley oligosaccharides and strain-Sakai showed weak binding ability to type Leb and Ley oligosaccharides. Our study presents new information and enhances understanding about the mechanism for NoV accumulation in oysters. Further studies of multiple NoV-tissue interactions might assist in identifying new or improved strategies for minimizing contamination, including HBGA-based attachment inhibition or depuration.

关键词： Crassostrea gigas norovirus histo-blood group antigen binding