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Peri-centrosomal localization of small interfering RNAs in C. elegans

Science China Life sciences 2025年

作者： Qile Jin Xuezhu Feng Minjie Hong Ke Wang Xiangyang Chen Jiewei Cheng Yan Kuang Xiaoyue Si Mingjing Xu Xinya Huang Shouhong Guang Chengming Zhu Department of Obstetrics and Gynecology The First Affiliated Hospital of USTC The USTC RNA Institute Hefei National Research Center for Physical Sciences at the Microscale School of Life Sciences Division of Life Sciences and Medicine Biomedical Sciences and Health Laboratory of Anhui Province University of Science and Technology of China School of Basic Medicine Anhui Medical University

The centrosome is the microtubule-organizing center and a crucial part of cell division. Centrosomal RNAs(cnRNAs) have been reported to enable precise spatiotemporal control of gene expression during cell division in many species. Whether and how cnRNAs exist in C. elegans are unclear. Here, using the nuclear RNAi Argonaute protein NRDE-3 as a reporter, we observed potential peri-centrosome localized small interfering(si)RNAs in C. elegans. NRDE-3 was previously shown to associate with pre-mRNAs and pre-rRNAs via a process involving the presence of complementary siRNAs. We generated a GFP-NRDE-3 knock-in transgene through CRISPR/Cas9 technology and observed that NRDE-3 formed peri-centrosomal foci neighboring the tubulin protein TBB-2, other centriole proteins and pericentriolar material(PCM)components in C. elegans embryos. The peri-centrosomal accumulation of NRDE-3 depends on RNA-dependent RNA polymerase(RdRP)-synthesized 22G siRNAs and the PAZ domain of NRDE-3, which is essential for siRNA binding. Mutation of eri-1, ergo-1, or drh-3 significantly increased the percentage of pericentrosome-enriched NRDE-3. At the metaphase of the cell cycle, NRDE-3 was enriched in both the peri-centrosomal region and the spindle. Moreover, the integrity of centriole proteins and pericentriolar material(PCM) components is also required for the peri-centrosomal accumulation of NRDE-3. Therefore, we concluded that siRNAs could accumulate in the pericentrosomal region in C. elegans and suggested that the peri-centrosomal region may also be a platform for RNAi-mediated gene regulation.

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Accurate manipulation of anthropomorphic robotic arm based on 3D human eye gazing with posture error compensation

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Science China Technological sciences 2025年

作者： Quanlin LI Xianzong WANG Jiaji MA Qianjun HUANG Qizhi JIANG Wenbin CHEN Caihua XIONG State key laboratory of Intelligent Manufacturing Equipment and Technology School of Mechanical Science and Engineering Huazhong University of Science and Technology Institute of medical Equipment Science and Engineering Huazhong University of Science and Technology

As humans and robots work closer together than ever, anthropomorphic robotic arms with intuitive human-robot interaction interfaces have drawn massive attention to improving the quality of robot-assisted manipulation. In pursuit of this,we designed a dedicated 7-degrees-of-freedom (DoF) anthropomorphic robotic arm having three compact differential joints and a head-mounted gaze tracker enabling head-pose-tracked 3D gaze estimation. Moreover, two key challenges were addressed to achieve accurate robot-assisted manipulation of the object indicated by the direction of human gaze. First, a novel predictive pupil feature was proposed for 3D gaze estimation. Differing from most existing features subjected to the common paraxial approximation assumption, the proposed novel predictive pupil feature considered the light refraction at two corneal surfaces with a more realistic eye model, significantly improving the 3D gaze estimation accuracy when the eyeball rotates at large angles. Second, a novel optimization-based approach was developed to efficiently compensate for the posture errors of the designed 7-DoF anthropomorphic robotic arm for accurate manipulation. Compared with the existing Jacobian-based or optimization-based approaches with nominal joint values as iteration initial, the proposed approach computed the optimal iteration initial and realized faster convergence for real-time posture error compensation. With the posture error compensation in real time and 3D gaze estimated accurately, the human can command accurate robot-assisted manipulation using his eyes intuitively. The proposed system was successfully tested on five healthy subjects.

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BEND3 takes the rein

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Science China(Life sciences) 2023年第5期66卷 1210-1212页

作者： Yang Yu State key laboratory of medical molecular biology Department of Molecular Biology and BiochemistryInstitute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

Transcription factors(TFs)interpret genome and guide gene expression through directly recognizing specific DNA sequences located in cis-elements and recruiting diverse transcription *** and DNA-binding motif interacti... 详细信息

关键词： diverse programs physiology

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Inflammation-induced CD69^+ Kupffer cell feedback inhibits T cell proliferation via membrane-bound TGF-β1

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Science China(Life sciences) 2016年第12期59卷 1259-1269页

作者： Xiang Zhang Zhengping Jiang Yan Gu Yanfang Liu Xuetao Cao Yanmei Han National key laboratory of medical Immunology & Institute of Immunology Second Military Medical University National key laboratory of medical molecular biology & Department of Immunology Institute of Basic Medical SciencesChinese Academy of Medical Sciences

Kupffer cells, tissue-resident macrophage lineage cell, are enriched in vertebrate liver. The mouse F4/80＋ Kupffer cells have been subclassified into two subpopulations according to their phenotype and function： CD68＋ subpopulation with potent reactive oxygen species （ROS） production and phagocytic capacities, and CD11b＋ subpopulation with a potent capacity to produce T helper 1 cytokines. In addition, CD11b＋ Kupffer cells/macrophages may be migrated from the bone marrow or spleen, especially in inflammatory conditions of the liver. For analyzing diverse Kupffer cell subsets, we infected mice with Listeria monocytogenes and analyzed the phenotype variations of hepatic Kupffer cells. During L. monocytogenes infection, hepatic CD69＋ Kupffer cells were significantly induced and expanded, and CD69＋ Kupffer cells expressed higher level of CD 1 lb, and particularly high level of membrane-bound TGF-β1 （mTGF-β1） but lower level of F4/80. We also found that clodronate liposome administration did not eliminate hepatic CD69＋ Kupffer cell subset. We consider the hepatic CD69＋ Kupffer cell population corresponds to CD11b＋ Kupffer cells, the bone marrow-derived population. Hepatic CD69＋ Kupffer cells suppressed Ag-nonspecific and OVA-specific CD4 T cell proliferation through mTGF-β1 both in vitro and in vivo, meanwhile, they did not interfere with activation of CD4 T cells. Thus, we have identified a new subset of inflammation-induced CD69＋ Kupffer cells which can feedback inhibit CD4 T cell response via cell surface TGF-β1 at the late stage of immune response against infection. CD69＋ Kupffer ceils may contribute to protect host from pathological injure by preventing overactivation of immune response.

关键词： Kupffer cell immune regulation CD69 TGF-β1 Listeria monocytogenes

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Differentiation of rhesus adipose stem cells into dopaminergic neurons

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Neural Regeneration Research 2012年第34期7卷 2645-2652页

作者： Yan Zhou Maosheng Sun Hongjun Li Min Yan Tianhong Xie molecular biology laboratory Institute of Medical Biology Chinese Academy of Medical Sciences & Peking Union Medical College

LIM homeobox transcription factor la （Lmxla） has the capacity to initiate the development program of neuronal cells and promote the differentiation of embryonic stem cells into dopaminergic neurons. In this study, rhesus adipose stem cells were infected with recombinant adenovirus carrying the Lmxla gene and co-cultured with embryonic rat neural stem cells. Cell differentiation was induced using sonic hedgehog and fibroblast growth factor-8. Immunofluorescence staining showed that cells were positive for neuron-specific enolase and ^-tubulin II1. Reverse transcription-PCR results demonstrated that rhesus adipose stem cells were not only positive for neuron-specific enolase and I^-tubulin III, but also positive for the dopaminergic neuron marker, tyrosine hydroxylase, neurofilament, glial cell line-derived neurotrophic factor family receptor a2 and nuclear receptor related factor 1. The number of Lmxla gene-infected cells expressing the dopaminergic neuron marker was substantially greater than the number of cells not infected with Lmxla gene. These results suggest that Lmxla-mediated regulation combined with the strategy of co-culture with neural stem cells can robustly promote the differentiation of rhesus adipose stem cells into dopaminergic neurons.

关键词： rhesus adipose stem cells differentiation LIM homeobox transcription factor la dopaminergicneurons co-culture neural stem cells Parkinson's disease neurodegenerative disease neuralregeneration

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Urinary Biomarkers of Brain Diseases

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Genomics, Proteomics & Bioinformatics 2015年第6期13卷 345-354页

作者： Manxia An Youhe Gao Department of Pathophysiology State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences School of Basic Medicine Peking Union Medical College Department of Biochemistry and molecular biology Beijing Normal University Beijing Key Laboratory of Gene Engineering and Biotechnology

Biomarkers are the measurable changes associated with a physiological or pathophysio- logical process. Unlike blood, urine is not subject to homeostatic mechanisms. Therefore, greater fluctuations could occur in urine than in blood, better reflecting the changes in human body. The roadmap of urine biomarker era was proposed. Although urine analysis has been attempted for clin- ical diagnosis, and urine has been monitored during the progression of many diseases, particularly urinary system diseases, whether urine can reflect brain disease status remains uncertain. As some biomarkers of brain diseases can be detected in the body fluids such as cerebrospinal fluid and blood, there is a possibility that urine also contain biomarkers of brain diseases. This review summarizes the clues of brain diseases reflected in the urine proteome and metabolome.

关键词： Brain diseases Urine Proteomics Metabolomics Biomarkers

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Based on non-targeted metabolomics for differential components screening of Rosae Chinensis Flos and Rosae Rugosae Flos and their quality evaluation

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Traditional Medicine Research 2025年第2期10卷 1-15页

作者： Xu Liang Ni-Hui Zhang Zhi-Lai Zhan Guang-Lu Chang Yan Gao Xia Li Wen-Yuan Gao School of Pharmaceutical Science and Technology Faculty of MedicineTianjin UniversityTianjin 300193China State key laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs China Academy of Chinese Medical SciencesBeijing 100700China key laboratory of Modern Chinese Medicine Resources Research Enterprises Tianjin 300402China

Background:Rosa chinensis *** Rosa rugosa *** not only of ornamental value,but also edible flowers and the flower buds have been listed in the Chinese Pharmacopoeia as traditional *** two plants have some differences in efficacy,but the flower buds are easily confused for similar *** addition,large-scale cultivation of ornamental rose flowers may lead to a decrease in the effective components of medicinal ***,it is necessary to study the chemical composition and make quality evaluation of Rosae Chinensis Flos(Yueji)and Rosae Rugosae Flos(Meigui).Methods:In this study,40 batches of samples including Meigui and Yueji from different regions in China were collected to establish high-performance liquid chromatography ***,the fingerprints data was analyzed using principal component analysis,hierarchical cluster analysis,and partial least squares discriminant analysis analysis chemometrics to obtain information on intergroup differences,and non-targeted metabolomic techniques were applied to identify and compare chemical compositions of samples which were chosen from groups with large *** compounds were screened by orthogonal partial least-squares discriminant analysis and S-plot,and finally multi-component quantification was performed to comprehensively evaluate the quality of Yueji and ***:The similarity between the fingerprints of 40 batches roses and the reference print R was 0.73 to 0.93,indicating that there were similarities and differences between the *** principal component analysis and hierarchical cluster analysis of fingerprints data,the samples from different origins and varieties were intuitively divided into four *** least-squares discriminant analysis analysis showed that Meigui and Yueji cluster into two categories and the model was reliable.A total of 89 compounds were identified by high resolution mass spectrometry,mainly were flavonoids and flavon

关键词： Rosa chinensis Jacq. Rosa rugosa Thunb. metabolomics chemometrics multiple component quantification quality evaluation

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Omics Approaches for Exploring Pneumoconiosis:A Review

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Biomedical and Environmental sciences 2021年第1期34卷 71-82页

作者： LUO Ya QI Xian Mei PANG Jun Ling WANG Jing WANG Chen State key laboratory of medical molecular biology Institute of Basic Medical SciencesChinese Academy of Medical SciencesDepartment of PathophysiologyPeking Union Medical CollegeBeijing 100730China State key laboratory of medical molecular biology Institute of Basic Medical SciencesChinese Academy of Medical SciencesDepartment of PhysiologyPeking Union Medical CollegeBeijing 100730China

Pneumoconiosis,a fatal lung disease caused by dust inhalation and deposition,is one of the leading occupational diseases worldwide[1].The dust is primarily inorganic particles,such as silica particles,coal dust,and asbestos *** the diameter of dust is less than 5μm,it is defined as‘respirable dust’that can easily reach the distal airways and alveoli[2].

关键词： lung diseases occupational

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一种用于动态三维细胞培养的具有可调控稳定压缩的仿生磁响应支架（英文）

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Science China Materials 2025年

作者：孙晓汪晓红武冰洁杨乾泓朱丛笑张慧敏李倩周宏儒郭明晖桂林李雷 key laboratory of Cryogenic Science and Technology Technical Institute of Physics and Chemistry Chinese Academy of Sciences School of Future Technology University of Chinese Academy of Sciences School of Biomedical Engineering Tsinghua University Plastic Surgery Hospital Chinese Academy of Medical Sciences Peking Union Medical College

磁响应支架能以快速、可逆和非接触的方式模拟体内细胞生长的动态三维微环境,因此被广泛用于组织工程领域.然而,受限于磁性纳米颗粒的弱磁性和水凝胶的机械脆性,现有的磁性支架很难为细胞提供与天然组织相似的动态压缩环境.为解决这一问...

磁响应支架能以快速、可逆和非接触的方式模拟体内细胞生长的动态三维微环境,因此被广泛用于组织工程领域.然而,受限于磁性纳米颗粒的弱磁性和水凝胶的机械脆性,现有的磁性支架很难为细胞提供与天然组织相似的动态压缩环境.为解决这一问题,我们提出了一种用于动态三维细胞培养的仿生磁性支架,为细胞提供可调且稳定的磁致压缩刺激.由硬磁颗粒NdFeB@SiO2和机械稳定的弹性体Ecoflex组成的磁性支架在磁场中（240 mT）可以产生高达15%的压缩应变.而且,磁性支架在4000次压缩循环中表现出优异的变形和机械稳定性.磁性支架还具有与脂肪组织相似的刚度（0.78 kPa）和粘弹性（弛豫时间为17 s）.实验表明,这种磁性支架不仅成功实现了人脂肪干细胞（hADSCs）的体外三维培养,还可以通过磁致压缩调节hADSCs的增殖.这种实现了动态三维细胞培养的磁性支架未来可能被应用于细胞生物学和组织工程研究.

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Inhibition of telomerase in tumor cells by ribozyme targeting telomerase RNA component

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Science China(Life sciences) 2002年第1期45卷 87-95页

作者：刘柏林屈艺欧阳雪松刘菽秋 laboratory of medical molecular biology West China University of Medical Sciences Chengdu China

Telomerase plays an important role in cell proliferation and carcinogenesis and is believed to be a good target for anti-cancer drugs. Elimination of template function of telomerase RNA may repress the telomerase activity. A hammer-headed ribozyme(telomerase ribozyme, teloRZ) directed against the RNA component of human telomerase(hTR) was designed and synthesized. TeloRZ showed a specific cleavage activity against the hTR. The cleavage efficacy reached 60%. A eukaryotic expression plasmid containing teloRZ gene was inducted into HeLa cells by lipofectamine, the telomerase activity in HeLa cells expressing teloRZ decreased to one eighth of that in the control cells. The doubling time increased significantly and the apoptosis ratio was elevated with increasing population doublings(PDS). After 19-20 PDS 95% cells were apoptotic. To further investigate the effect of teloRZ on tumor growth, the eukaryotic expression plasmid containing teloRZ was injected into transplanted tumor of nude mouse. The teloRZ effectively inhibited the telomerase activity in transplanted tumor, promoted apoptosis of the transplanted tumor cells, and decreased the tumor size significantly. These results indicate that teloRZ can effectively inhibit telomerase activity and growth of tumor cells, and suggest the potential use of this ribozyme in anti-cancer therapy.

关键词： ribozyme, telomerase, HeLa cells, cleavage, inhibition.

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