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Investigation of genome instability in patients with non-alcoholic steatohepatitis

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World Journal of Gastroenterology 2013年第32期19卷 5295-5301页

作者： Ahmet Karaman Hamiyet Donmez-Altuntas Nazmiye Bitgen Zuhal Hamurcu Arzu Oguz Cigdem Karakukcu Department of Gastroenterology Kayseri Education and Research Hospital Department of Medical Biology Medical SchoolErciyes University Department of Medical Oncology Kayseri Education and Research Hospital Department of Clinical Biochemistry Kayseri Education and Research Hospital

AIM:To evaluate the occurrence of micronucleus(MN),nucleoplasmic bridges(NPBs)and nuclear buds(NBUDs)in the mitogen-stimulated lymphocytes of patients with non-alcoholic steatohepatitis(NASH).METHODS:The study was performed in 25(9 females,16 males)patients newly diagnosed with NASH,and 25healthy subjects of similar ages and genders were used as a control *** of the controls was known to be receiving any drugs for medical or other reasons or using *** was further excluded by abdominal ultrasound imaging in the control *** numbers of MN,NPBs and NBUDs scored in binucleated(BN)cells were obtained from the mitogen-stimulated lymphocytes of patients and control *** comparisons of the numbers of BN cells with MN,NPBs and NBUDs and ages between the patients with NASH and control subjects were ***:The mean ages of the patients and the control group were 41.92±13.33 and 41.80±13.09 years(P>0.05),*** values of the mean body mass index(BMI),HOMA-IR,hemoglobin,creatinin,aspartate aminotransferase,alanine aminotransferase,triglyceride,high density lipoprotein,and low density lipoprotein were 31.19±4.62 kg/m2vs 25.07±4.14 kg/m2,6.71±4.68 vs 1.40±0.53,14.73±1.49 g/dL vs 14.64±1.30 g/dL,0.74±0.15 mg/dL vs 0.80±0.13 mg/dL,56.08±29.11 U/L vs 16.88±3.33 U/L,92.2±41.43U/L vs 15.88±5.88 U/L,219.21±141.68 mg/dL vs102.56±57.98 mg/dL,16.37±9.65 mg/dL vs 48.72±15.31 mg/dL,and 136.75±30.14 mg/dL vs 114.63±34.13 mg/dL in the patients and control groups,*** total numbers and frequencies of BN cells with MN,NPBs and NBUDs,which were scored using the CBMN cytome assay on PHA-stimulated lymphocytes,were evaluated in the patients with NASH and control *** found significantly higher numbers of MN,NPBs and NBUDs in the BN cells of patients with NASH than in those of the control subjects(21.60±9.32vs 6.88±3.91;29.28±13.31 vs 7.84±3.96;15.60±5.55 vs 4.20±1.63,respectively,P<0.0001).CONCLUSION:The increased

关键词： Non-alcoholic steatohepatitis Micronucleus Nucleoplasmic bridges Nuclear buds

Effects of moxifloxacin exposure on the conjunctival flora and antibiotic resistance profile following repeated intravitreal injections

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International Journal of Ophthalmology(English edition) 2014年第5期7卷 855-859页

作者： Mustafa Atas Burhan Baskan Ayse zkse Fatma Mutlu Sarιgüzel Süleyman Demircan Emine Pangal Department of Ophtalmology Kayseri Education and Research Hospital Department of Microbiology Kayseri Education and Research Hospital

AIMTo evaluate the effects of moxifloxacin exposure on the conjunctival flora and antibiotic resistance profile following repeated intravitreal injections.

关键词： intravitreal injection moxifloxacin endopthalmitis

Measurement of choroidal thickness and macular thickness during and after pregnancy

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International Journal of Ophthalmology(English edition) 2015年第2期8卷 321-325页

作者： DondüMelek Ulusoy Necati Duru Mustafa Atas Hasan Altinkaynak Zeynep Duru Gokhan Acmaz Department of Ophthalmology Kayseri Education and Research Hospital Department of Ophthalmology Ankara Atatürk Education and Research Hospital Department of Ophthalmology Ankara Numune Education and Research Hospital Department of Obstetrics and Gynecology Kayseri Education and Research Hospital

AIM: To investigate the effect of pregnancy on subfoveal choroidal thickness(SFCT) and macular thickness in both pregnant and not pregnant healthy ***: Twenty-nine healthy pregnant women in their third trimester and 36 age-matched healthy women were enrolled in a prospective, cross-sectional *** and parafoveal thickness in the four quadrants and SFCT were measured by optical coherence tomography(OCT) in the healthy pregnant women(*** group) and healthy women(i.e. control group).OCT measurements were again measured 3mo after delivery in the study ***: Mean SFCT measurements in the control group, pregnant women of the study group, and after delivery of the study group were 320.86 ±59.18 μm,387.97 ±59.91 μm, and 332.40 ±26.03 μm, *** was a statistically significant difference in the mean SFCT values between pregnant women of the study group and the control group(P =0.000). Foveal and parafoveal thickness values were not statistically significant in either the study or control ***: SFCT increases during pregnancy and returns to normal range in the three months after delivery. Macular thickness does not show any change during pregnancy.

关键词： enhanced depth imaging optical coherence tomography pregnancy choroidal thickness foveal thickness

Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin Dong Bin Duan Feiyang Xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University research Institute of Intelligent Computing Zhejiang Lab Shanghai research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

Iatrogenic scleroderma renal crisis:A case report and mini literature review for renal crisis

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Discussion of Clinical Cases 2021年第3期8卷 4-8页

作者： Gülsah Boz Samet Karahan Nurdan Yüksek kayseri city education and research hospital Department of Internal MedicineDivision of NephrologyKayseriTurkey kayseri city education and research hospital Department of Internal MedicineDivision of RheumatologyKayseriTurkey kayseri city education and research hospital Department of Internal MedicineKayseriTurkey

Background:Systemic sclerosis is a connective tissue disease characterized by fibrosis of the skin and organs,marked changes in microvascular structure,cellular and humoral immune *** involvement is more frequent and mainly characterized by moderate proteinuria,elevated serum creatinine levels,and *** most common kidney involvement in SSc is scleroderma renal crisis(SRC)that is fatal without prompt *** report:A 52-year-old Caucasian male with known diffuse cutaneous systemic sclerosis was hospitalized with communityacquired *** the fifth day after appropriate antibiotic therapy and 60 mg/day methylprednisolone,decreased urine output,arterial hypertension,decreased renal function and pulmonary edema *** patient was diagnosed with a scleroderma renal *** hemodialysis was applied to the patient,and captopril 6×25 mg/day and nifedipine 2*60 mg/day treatment were *** received a routine hemodialysis program for about three *** hemodialysis program was terminated when the patient’s urine quality and quantity ***:SRC,characterized by malignant hypertension,azotemia,microangiopathic hemolytic anemia,and kidney failure,is one of the most important complications of systemic sclerosis with a poor prognosis without prompt *** use is one of the important risk factors that precipitate SRC *** angiotensin-converting enzyme inhibitors,survival increased after SRC,the need for dialysis decreased,and usually allowed the discontinuation of dialysis treatment within about 6-18 *** of SRC in the presence of the above-mentioned findings in patients with a diagnosis or suspected systemic sclerosis can be considered the most important treatment step.

关键词： Scleroderma Systemic sclerosis Scleroderma renal crisis Systemic sclerosis-associated thrombotic microangiopathy

Nosocomial infection and spread of SARS-CoV-2 infection among hospital staff,patients and caregivers

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World Journal of Clinical Cases 2022年第34期10卷 12559-12565页

作者： Chih-Chien Cheng Li-Yun Fann Yi-Chang Chou Chia-Chen Liu Hsiao-Yun Hu Dachen Chu Department of education and research Taipei City HospitalTaipei 116009Taiwan Department of Nursing Taipei City HospitalTaipei 106243Taiwan Department of education and research Taipei City HospitalTaipei 106109Taiwan School of Medicine College of MedicineFu Jen Catholic UniversityTaipei 106109Taiwan

BACKGROUND There are difficulties in diagnosing nosocomial transmission of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) infection in hospital settings. Furthermore, mortality of cases of nosocomial infection(NI) with SARS-CoV-2 is higher than that of the general infected population. In the early stage of the pandemic in Taiwan, as patients were not tested for SARS-CoV-2 at admission, NIs often go undetected. Strictly applying the systematic polymerase chain reaction(PCR) screening, as a standard infection control measure was subsequently implemented to reduce NI incidence. However, evidence on risk factors for SARS-CoV-2 NIs among healthcare workers(HCWs) and caregivers is *** To assess NI incidence of SARS-CoV-2 among hospital staff, hospitalized patients, and caregivers, and the transmission routes of clusters of *** This descriptive retrospective analysis at our hospital from May 15 to August 15, 2021 included data on 132 SARS-CoV-2 NIs cases among hospital staff, inpatients, and caregivers who previously tested negative but subsequently identified with a positive SARS-CoV-2 reverse transcriptase-PCR(RT-PCR) test results, or a hospital staff who tested positive following routine SARS-CoV-2 RT-PCR test. Chi-square tests were performed to compare the differences between hospital staff and private caregivers, and between clusters and sporadic *** Overall, 9149 patients and 2005 hospital staff members underwent routine SARS-CoV-2 RT-PCR testing, resulting in 12 confirmed cluster and 23 sporadic infections. Among the index cases of the clusters, three(25%) cases were among hospital staff and nine(75%) cases were among other contacts. Among sporadic infections, 21(91%) cases were among hospital staff and two(9%) cases were among other contacts(P hospital staff(30%), and caregivers(25%). Private

关键词： COVID-19 SARS CoV-2 Cross infection Incidence Retrospective studies Infection control

Tranylcypromine upregulates Sestrin 2 expression to ameliorate NLRP3-related noise-induced hearing loss

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Neural Regeneration research 2025年第5期20卷 1483-1494页

作者： Xihang Chen Zhifeng Chen Menghua Li Weiwei Guo Shuolong Yuan Liangwei Xu Chang Lin Xi Shi Wei Chen Shiming Yang Senior Department of Otolaryngology Head and Neck Surgery Chinese PLA General HospitalChinese PLA Medical SchoolBeijingChina State Key Laboratory of Hearing and Balance Science BeijingChina National Clinical research Center for Otolaryngologic Diseases BeijingChina Key Laboratory of Hearing Science Ministry of EducationBeijingChina Beijing Key Laboratory of Hearing Impairment Prevention and Treatment BeijingChina Department of Otorhinolaryngology Head and Neck Surgery The First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Otolaryngology Head and Neck Surgery The 940th Hospital of Joint Logistics Support Force of Chinese PLALanzhouGansu ProvinceChina Key Laboratory of Tropical Biological Resources of Ministry of education School of Pharmaceutical SciencesHainan UniversityHaikouHainan ProvinceChina Academician Workstation of Hainan University School of Pharmaceutical SciencesYazhou BaySanyaHainan ProvinceChina

Noise-induced hearing loss is the primary non-genetic factor contributing to auditory ***,there are currently no effective pharmacological interventions for patients with noise-induced hearing ***,we present evidence suggesting that the lysine-specific demethylase 1 inhibitor–tranylcypromine is an otoprotective agent that could be used to treat noise-induced hearing loss,and elucidate its underlying regulatory *** established a mouse model of permanent threshold shift hearing loss by exposing the mice to white broadband noise at a sound pressure level of 120 d B for 4 *** found that tranylcypromine treatment led to the upregulation of Sestrin2(SESN2)and activation of the autophagy markers light chain 3B and lysosome-associated membrane glycoprotein 1 in the cochleae of mice treated with *** noise exposure group treated with tranylcypromine showed significantly lower average auditory brainstem response hearing thresholds at click,4,8,and 16 k Hz frequencies compared with the noise exposure group treated with *** findings indicate that tranylcypromine treatment resulted in increased SESN2,light chain 3B,and lysosome-associated membrane glycoprotein 1 expression after noise exposure,leading to a reduction in levels of 4-hydroxynonenal and cleaved caspase-3,thereby reducing noise-induced hair cell ***,immunoblot analysis demonstrated that treatment with tranylcypromine upregulated SESN2 expression via the autophagy *** treatment also reduced the production of NOD-like receptor family pyrin domaincontaining 3(NLRP3)*** conclusion,our results showed that tranylcypromine treatment ameliorated cochlear inflammation by promoting the expression of SESN2,which induced autophagy,thereby restricting NLRP3-related inflammasome signaling,alleviating cochlear hair cell loss,and protecting hearing *** findings suggest that inhibiting lysine-specific demethylase 1 is a

关键词： 4-hydroxynonenal apoptosis autophagy cleaved caspase-3 inflammation NOD-like receptor family pyrin domain-containing 3(NLRP3) noise-induced hearing loss oxidative stress Sestrin2 tranylcypromine

Interaction between Immunotherapy and Radiotherapy

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Journal of Oncology research 2019年第1期1卷 14-15页

作者： Yasemin Benderli Cihan kayseri education and research hospital Department of Radiation Oncology38010KayseriTurkey

In recent years,treatment methods on immune checkpoints have emerged as promising novel therapeutic modalities against cancer as a result of studies focusing on elucidation of immune *** has now become an established treatment in some cancers.[1-2]This has led the need for investigation of biomarkers which allow determining effectiveness of immunotherapies and patient groups which will most benefit from these *** previous studies,it was suggested that programmed death receptor-1(PD-1)and programmed death ligand-1(PD-L1)expressions could be predictive biomarkers in ***-1 is a transmembrane protein present in macrophages,myeloid dendritic cells,B cells,epithelial cells and vascular endothelial cells,which limits and inhibits immunological activation in activated T *** PD-1/PDL1 interaction promises hope in the cancer *** clinical studies,it was shown that targeted PD-1/PD-L1 therapy alone or in combination with other modalities is beneficial in advanced cancers with aggressive *** was shown that overexpression of PD-1 present in tumoral micro-environment is associated to poor prognosis in gastric cancer,breast cancer,ovarian cancer,kidney,pancreas and lung cancers and in melanoma.[1-5].

关键词： Immunotherapy Radiotherapy

Is There Any Threshold for Vitamin D That Elevates Parathyroid Hormone

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Open Journal of Endocrine and Metabolic Diseases 2017年第3期7卷 97-110页

作者： Meral Mert Gonca Tamer Nahide Ekici Gunay Ilkay Kartal Hamide Piskinpasa Pinar Karakaya Yildiz Okuturlar Clinic of Endocrinology and Metabolism Bakirkoy Dr. Sadi Konuk Education and Research Hospital Istanbul Turkey Clinic of Endocrinology and Metabolism Istanbul Medeniyet University Goztepe Education and Research Hospital Istanbul Turkey Central Laboratory Kayseri Education and Research Hospital Kayseri Turkey Clinic of Internal Medicine Bakirkoy Dr. Sadi Konuk Education and Research Hospital Istanbul Turkey

Objective: To evaluate vitamin D status in relation to serum levels for parathyroid hormone (PTH) and corrected calcium among endocrine outpatients. Methods: A total of 760 patients (mean(SD) age: 40.0(12.6) years, 94.6% were females) admitted to our endocrinology outpatient clinic were included and evaluated with respect to patient demographics, serum levels for 25-hydroxyvitamin D (25(OH) D, ng/mL), PTH (pg/mL) and corrected calcium (mg/dL) as well as the vitamin D status. Results: Vitamin D deficiency was determined in 65.0% and elevated PTH levels in 20.3% of patients. iPTH levels were significantly higher in females than in males (58.9(40.4) vs. 45.1(26.2) pg/mL, p = 0.031) and in summer than in winter cases (63.9(47.7) vs. 54.2(32.8) pg/mL, p = 0.002), whereas no difference was found in serum levels for 25(OH) D, corrected calcium and phosphate with respect to gender and season. Significantly higher levels for iPTH were noted in vitamin D deficient patients (60.7(43.9) pg/mL) than in normal (51.1(33.4) pg/mL) and vitamin D insufficient (57.1(26.0) pg/mL) cases (p = 0.03). iPTH levels were correlated positively with age (r = 0.116, p = 0.001) and negatively with corrected calcium (r = −0.097, p = 0.008), P (r = −0.224, p = 0.000) and 25(OH) D (r = −0.134, p = 0.000), whereas no correlation was noted between 25(OH) D and corrected calcium levels. Conclusion: Our findings indicated that vitamin D deficiency in 65.0%, whereas PTH elevation only in 20.3% of endocrine outpatients, despite the significantly negative correlation of PTH to 25(OH) D and significantly higher levels of PTH among vitamin D deficient cases than in vitamin D insufficient and sufficient cases. Gender’s and seasonal differences had influence on serum levels for PTH but not on either 25(OH) D or corrected calcium, and no correlation was evident between 25(OH) D and corrected calcium levels.

关键词： Vitamin D Deficiency Parathyroid Hormone Calcium 25(OH) D Testing