检索结果-南通市图书馆

Effects of NaCI and Ca^(2+) on Membrane Potential of Epidermal Cells of Maize Roots

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2008年第3期7卷 291-296页

作者： HUA Jia-min WANG Xiao-li ZHAI Fu-qin YAN Feng FENG Ke Key Lab of Crop Genetics and Physiology of Jiangsu Province Yangzhou University Yangzhou 225009 P.R.China Department of Plant Production and Plant Breeding Justus-Liebig University Giessen D-35390 Germany

The effects of salt-stress on plants involve not only the water stress caused by low osmotic pressure, but also the toxicity of excess Na^＋. A large amount of Na^＋ entering cells would reduce K^＋ uptake, which leads to an imbalance of K：Na ratio in cells. One of the reasons for the reduced K^＋-uptake is the closure of K^＋-channel which is controlled by membrane potential. Calcium is usually applied to improve the growth of plants on saline soils and shows positive influence in the integrality of cell membrane. This study applied glass microelectrode technique to monitoring the NaCl-induced changes of membrane potential of root epidermal cells of maize （Zea mays L., Denghai 11） seedlings at NaCl concentrations of 0, 8, 20, 50, 100, 200 mmol L^-1, respectively. The effect of Ca^2＋ on the changes of membrane potential caused by NaCl was also studied. The results showed that： NaCl caused cell membrane depolarization. The depolarization became greater and faster with increasing of NaCl concentration. Moreover, the extent of depolarization was positively correlated with NaCl concentration. The addition of calcium postponed the depolarization, and decreased the degree of depolarization caused by NaCl. High NaCl concentration leads to depolarization of maize root cell membrane, which can partly be counteracted by calcium.

关键词： membrane potential sodium chloride calcium epidermal cells maize root

Neuropharmacological Characterization of Extracts from Rhodiola rosea, Oenothera paradoxa and Paullinia cupana in Comparison to Caffeine

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Pharmacology & Pharmacy 2016年第7期7卷 290-303页

作者： Wilfried Dimpfel Leonie Schombert Tanausú Vega-Morales Julia Charlotte Wiebe justus-liebig-university giessen Giessen Germany NeuroCode AG Wetzlar Germany PoliNat Polifenoles Naturales Las Palmas Spain

To find possible therapeutic applications involving the Central Nervous System (CNS) for herbals is a major challenge during functional food and drug discovery and development programmes. Despite the availability of numerous in vitro and in vivo tests, there is no single agreed screening procedure for pharmacological testing of herbal extracts with anticipated CNS activity. Experience gained from more than 25 years of testing has shown that two models give reasonably reliable orientation for future CNS applications: construction of an electropharmacogram based on wireless recording of field potentials from the depth of the brain of freely moving rats (Tele-Stereo-EEG) and recording of the population spike produced by pyramidal cells from hippocampal slices in vitro. A combination of these two methods has now been used to characterize the pharmacological profile of extracts from Rhodiola rosea root, Oenothera paradoxa seeds and Paullinia cupana seeds. Spectral analysis of field potentials revealed attenuation of alpha2 and beta1 waves was common for all extracts. According to previous studies, this is interpreted as activation of the dopaminergic and glutamatergic transmission. In addition, Oenothera and Rhodiola extracts attenuated delta and theta power, probably related to interference with the cholinergic and norepinephrinergic transmission, respectively. Using discriminant analysis for comparison with reference pharmaceutical and botanical drugs, Rhodiola projected near the position of Ginkgo extract, whereas Oenothera extract was projected near the position of Tramadol, an analgesic drug. Physical motion was increased only in the presence of Paullinia extract and caffeine. Increases of long-term potentiation were observed in the presence of Rhodiola extract, Paullinia extract and caffeine. The combined information predicts stimulant and cognitive function-enhancing activities in humans for the Rhodiola extract, which could also be used as a possible caffeine-rep

关键词： Pharmacology Field Potentials Hippocampal Slices Rhodiola rosea Oenothera paradoxa Paullinia cupana Caffeine RhodioLife® Discriminant Analysis

High-Performance Liquid Chromatography Fingerprint of Marine Traditional Chinese Medicine Haliotidis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Traditional Chinese Medicine 2022年第3期8卷 446-452页

作者： Qing Zhao Shu-Yue Song Meng-Qi Zhang Xin Li Yang Liu Chang-Yun Wang Key Laboratory of Marine Drugs The Ministry of Education of ChinaSchool of Medicine and PharmacyOcean University of ChinaLaboratory for Marine Drugs and BioproductsQingdao National Laboratory for Marine Science and TechnologyQingdaoChina Insect Biotechnology Justus Liebig University GiessenGiessenGermany

Objective: Haliotidis, the dried muscle of abalone, has long been used as a marine traditional Chinese medicine(TCM) due to its high medicinal and nutritive values, whereas few reports contributed to its quality control. This study aimed to establish the high-performance liquid chromatography(HPLC) fingerprints of Haliotidis. Materials and Methods: The HPLC fingerprints of Haliotidis, the manufacturing products of dried muscle of abalone, were analyzed by reverse-phase HPLC, using a YMC HPLC C18 column(250 mm × 4.6 mm, 5 μm).The gradient elution mode was applied with mobile phase starting from 5% acetonitrile and 0.1% phosphoric acid aqueous solution to 100%acetonitrile in 70 min at a flow rate of 1.0 mL/min. The detection wavelength was set at 190 nm. Results: Through compared the HPLC fingerprint chromatograms of 14 batches of Haliotidis, 25 peaks were assigned as the characteristic common peaks, of which one index component L-phenylalanine was identified by comparison with its standard substance. The established HPLC fingerprint analysis method of Haliotidis was validated with good precision, repeatability, and stability. Based on the above study, a reference HPLC fingerprint chromatogram of Haliotidis was proposed. Conclusions: It is the first time to establish the HPLC fingerprint chromatogram of TCM Haliotidis, which could contribute to the establishment of its quality control system.

关键词： Marine traditional Chinese medicine Haliotidis HPLC fingerprint quality control

Characterization of seedling proteomes and development of markers to distinguish the Brassica A and C genomes

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2010年第5期37卷 333-340页

作者： Fang Kong Cailin Ge Xiaoping Fang Rod J. Snowdon Youping Wang College of Bioscience and Biotechnology Yangzhou University Yangzhou 225009 China Oil Crops Research Institute Chinese Academy of Agricultural Sciences Wuhan 430062 China Institute for Plant Breeding and Crop Science I Justus Liebig University Heinrich-Buff-Ring 26-32 Giessen 35392 Germany

The diploid species Brassica rapa （genome AA） and B. oleracea （genome CC） were compared by full-scale proteome analyses of seedling. A total of 28.2% of the proteins was common to both species, indicating the existence of a basal or ubiquitous proteome. However, a number of discriminating proteins （32.0%） and specific proteins （39.8%） of the Brassica A and C genomes, respectively, were identified, which could represent potentially species-specific functions. Based on these A or C genome-specific proteins, a number of PCR-based markers to distinguish B. rapa and B. oleracea species were also developed.

关键词： Brassica rapa Brassica oleracea proteomics molecular marker

Evidence for lung repair and regeneration in humans: key stem cells and therapeutic functions of fibroblast growth factors

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Frontiers of Medicine 2020年第3期14卷 262-272页

作者： Xuran Chu Chengshui Chen Chaolei Chen Jin-San Zhang Saverio Bellusci Xiaokun Li School of Pharmaceutical Sciences Wenzhou Medical UniversityWenzhou 325035China Cardio-Pulmonary Institute Universities of Giessen and Marburg Lung Centermember of the German Center for Lung ResearchJustus-Liebig-University Giessen35392 GiessenGermany Laboratory of Interventional Pulmonology of Zhejiang Province Department of Pulmonary and Critical Care MedicineThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000China Institute of Life Sciences Wenzhou UniversityWenzhou 325035China

Regeneration carries the idea of regrowing partially or completely a missing ***,on the other hand,allows restoring the function of an existing but failing *** recognition that human lungs can both repair and regenerate is quite novel,the concept has not been widely used to treat *** present evidence that the human adult lung does repair and regenerate and introduce different ways to harness this *** types of lung stem cells are capable of proliferating and differentiating upon injury driving the repair/regeneration *** models,primarily in mice,combined with lineage tracing studies,have allowed the identification of these important *** of these cells,such as basal cells,broncho-alveolar stem cells,and alveolar type 2 cells,rely on fibroblast growth factor (FGF) signaling for their survival,proliferation and/or *** preclinical studies have shown the therapeutic benefits of FGFs,a recent clinical trial for acute respiratory distress syndrome (ARDS) using intravenous injection of FGF7 did not report the expected beneficial *** discuss the potential reasons for these negative results and propose the rationale for new approaches for future clinical trials,such as delivery of FGFs to the damaged lungs through efficient inhalation systems,which may be more promising than systemic exposure to *** this change in the administration route presents a challenge,the therapeutic promises displayed by FGFs are worth the effort.

关键词： FGF human lung repair regeneration stem cells

In VitroHippocampal Electrophysiology and in VivoQuantitative EEG Revealed Robust Neurophysiological Effects of the Antivertigo-Agent Vertigoheel®in a Rat Study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neuroscience & Medicine 2019年第4期10卷 407-425页

作者： Wilfried Dimpfel Bernd Seilheimer Leonie Schombert justus liebig university giessen Giessen Germany Biologische Heilmittel Heel GmbH Baden-Baden Germany NeuroCode AG Wetzlar Germany

Vertigo is a common symptom with impact on daily life. Vertigoheel®(VH-04) has demonstrated to be effective for Vertigo in former studies. This paper aims to investigate the mode of action of the medicinal product VH-04 in the rat brain. In an in vitro study neurophysiological recording from hippocampal slices from adult male Sprague Dawley®rats was performed in order to substantiate a possible direct effect on the brain of VH-04 in different concentrations. In an in vivo cross-over study with 11 Fischer 344®?rats, a neurophysiological method was applied to systemically analyse VH-04’s activity in the rat brain. This method combines quantitative assessments of telemetrically transmitted field potentials after drug treatment with subsequent discriminant analysis to classify the compound. The database used for the analysis of classification contained numerous chemicals and medicinal products of different dosages, all tested in the same paradigm, which is continuous wireless monitoring of the EEG of freely moving rats before and after drug intake. Following single stimuli on the Schaffer collaterals in the presence of VH-04 in different concentrations, in vitro responses of pyramidal cells increased depending on the VH-04 concentration (0.25 - 4 ml/L). Results were statistically significant for concentrations above 2.5 ml/L. Long-term potentiation was only marginally affected. Out of several specific glutamate receptor antagonists the effect of VH-04 was only antagonized by AMPA and kainic acid receptor-mediated signalling. Their enhancement indicates better information processing in the hippocampus, a brain structure primarily involved in memory processes. The in vivo characterisation of VH-04-induced changes in EEG-signatures of four brain areas (the frontal cortex (FC), the hippocampus (HC), the striatum (ST) and the reticular formation (RF)) revealed a dose-dependent attenuation of delta, theta, alpha 2 and beta 1 waves. The subsequent discriminant f

关键词： EEG Rat Model Central Nervous System Vertigo VH-04 Neurophysiology Hippocampus Multitarget Fast Fourier Transformation

Psychophysiological Effects of Sideritis and Bacopa Extract and Three Combinations Thereof—A Quantitative EEG Study in Subjects Suffering from Mild Cognitive Impairment (MCI)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Alzheimer's Disease 2016年第1期5卷 1-22页

作者： Wilfried Dimpfel Leonie Schombert Andreas Biller justus-liebig-university giessen Giessen Germany Neuro Code AG Wetzlar Germany Dr. Loges GmbH Winsen Germany

Mild cognitive impairment (MCI) is regarded as a transitional stage during the development of Alzheimer’s disease. Diagnosis of MCI can be obtained by the questionnaire “DemTect” in German speaking countries. Quantitative assessment has been successfully performed using psychometric testing concomitantly with quantitative EEG recording. The present investigation aimed at the possible treatment of MCI with two botanicals, namely extracts from Sideritis scardica (500 mg) or Bacopa monnieri (320 mg) and three combinations thereof using this method in order to find a new treatment. The performance of the d2-test, an arithmetic calculation test (CPT) and a memory-test revealed better performance for the d2-test only in the presence of Sideritis extract or the combinations with Bacopa extract. Quantitative EEG assessment during the different experimental conditions showed massive differences between both extracts. Whereas Sideritis extract and its combination with a low amount of Bacopa extract (160 mg) induced increases of spectral power in fronto-temporal brain areas, Bacopa and the combination of Sideritis with high amounts of Bacopa extract produced attenuation of all waves except for delta in fronto-temporal brain areas. These differences were also documented by quantitative EEG maps in comparison to Placebo. A different action of both extracts was confirmed by discriminant analysis, where Sideritis extract and its combination with low Bacopa grouped together quite at distance to Bacopa and the combination of Sideritis with high Bacopa. A combination of Sideritis extract with a low amount of Bacopa should be tested with daily repetitive dosing for at least 4 weeks as a consequence.

关键词： DemTect Cognition Psychometry EEG Source density Mild Cognitive Impairment (MCI) Alzheimer’s Disease CATEEM Sideritis Bacopa

CTX-M producing Escherichia coli isolated from cattle feces in Bogor slaughterhouse,Indonesia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2016年第7期6卷 605-608页

作者： Mirnawati Bachrum Sudarwanto Denny Widaya Lukman Hadri Latif Herwin Pisestyani Eddy Sukmawinata mer Akineden Ewald Usleber Laboratory of Veterinary Public Health Faculty of Veterinary MedicineBogor Agricultural University Veterinary Public Health Programme Veterinary Public HealthGraduate SchoolBogor Agricultural University Dairy Sciences Institute of Veterinary Food ScienceJustus-Liebig University Giessen

Objective: To determine the occurrence of CTX-M producing Escherichia coli(E. coli)from cattle feces in Bogor slaughterhouse, ***: A total of 220 cattle feces samples were collected from Bogor slaughterhouse from March to April 2015. Presence of extended-spectrum beta-lactamase(ESBL) producing E. coli was detected by disc diffusion test based on the recommendation from Clinical and Laboratory Standards Institute(2014). Bacterial strains which were confirmed as producing ESBLs were further analyzed for the presence of bla genes of the ESBL by ***: The results showed that CTX-M producing E. coli isolates were detected in 19 samples from 220 samples(8.6%). The b-lactamase genes detected were CTX-M-1(n = 10) and CTX-M-9(n = 9). All of the CTX-M producing E. coli isolates showed multidrug resistance phenotypes to at least four antibiotics. The highest incidence of antibiotics resistance was showed to ampicillin(100.0%), cefotaxime(100.0%), and cefpodoxime(100.0%), followed by streptomycin(84.3%), trimethoprim-sulfamethoxazole(73.7%), erythromycin(52.6%), kanamycin(26.3%), doxycycline(10.5%), and ceftazidime(0.0%).Conclusions: Detection of CTX-M-producing E. coli in cattle feces raises important questions as they can represent a potential risk factor to public health.

关键词： Cattle feces CTX-M Escherichia coli Slaughterhouse

Chromatin-targeting of de novo DNA cytosine methyltransferases by PWWP domain

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chromatin-targeting of de novo DNA cytosine methyltransferas...