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Protein & Cell 2016年第12期7卷 913-915页

作者： Shawn Burgess Linzhao Cheng Feng Gu Zhiwei Huang Shuo Lin Jinsong Li Wei Li Wei Qin Yujie Sun Zhou Songyang Wensheng Wei Qiang Wu Haoyi Wang Xiaoqun Wang Jing-Wei Xiong Jianzhong Xi Hui Yang Bin Zhou Bo Zhang Junjiu Huang National Human Genome Research Institute NIH Bethesda MD 20892 USA Division of Hematology in Department of Medicine Johns HopkinsUniversity School of Medicine Baltimore MD 21205 USA Stem Cell Program in the Institute for Cell Engineering Johns Hopkins University School of Medicine Baltimore MD 21205 USA center for Vision Research Eye Hospital Wenzhou Medical University Wenzhou 325035 China Stem Cell and Functional Genomics Laboratory School of LifeSciences Sun Yat-sen University Guangzhou 510275 China HIT center of Life Sciences Harbin Institute of Technology Harbin 150080 China Department of Molecular Cell and Developmental Biology University of California Los Angeles Los Angeles CA 90095 USA Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China State Key Laboratory of Stem cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China College of Chemical Biology and Biotechnology Peking Univer- sity Shenzhen Graduate School Shenzhen 518055 China PKU BIOPIC Beijing 100871 China BIOPIC ICG CLS and School of Life Sciences Peking Univer-sity Beijing 100871 China center for Comparative Biomedicine Institute of SystemsBiomedicine Shanghai Jiao Tong University Shanghai 200240China Institute of Biophysics Chinese Academy of Sciences Beijing100101 China Institute of Molecular Medicine Peking University Beijing100871 China College of Engineering Peking University Beiiing 100871 China Laboratory of Disease Models in Non-Human Primates Instituteof Neuroscience Shanghai Chinese Academy of SciencesShanghai 200031 China College of Life Sciences Peking University Beijing 100871 China

Dear Editor： Gao et al. published data in Nature Biotechnology （Nat Biotechnol. 2016 May 2） showing that DNA-guided genome editing using the Natronobacterium gregoryi Argonaute （NgAgo） protein targeted 47 mammalian genomic loci with a 100% success rate and an efficiency of 21.3%-41.3% at various targets. This report led us to test NgAgo＇s utility in various cells and organisms such as mouse and zebrafish for gene editing.

关键词： Supplementary Information Genomic Locus Genome Editing Gene Editing Online Forum

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A review: status and prospect of heterosis prediction in rapeseed(Brassica napus L.)

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Oil Crop Science 2017年第3期2卷 187-194页

作者： Lifang Wang Fang Zhang Tingdong Fu Jinxiong Shen Key Labortory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture Oil Crops Research InstituteChinese Academy of Agricultural Sciences Wuhan China College of Plant Science and Technology Huazhong Agricultural University Wuhan China graduate School Chinese Academy of Agricultural Sciences Beijing China The National Agro-tech extension and Service center Beijing China

Heterosis has been widely exploited and utilized in rapeseed breeding. Reliable and precise prediction techniques of heterosis contributed in accelerating the crossbreeding and reducing the cost of large-scale field evaluation. Combined with our work in last decades, this review summarized the progress on heterosis prediction of rapeseed (Brassica napus L.), compared the efficiency of different methods, and proposed its prospect.

关键词： Brassica napus L. heterosis prediction combining ability molecular markers

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Lymph node ratio and preoperative CA 19-9 levels predict overall survival and recurrence-free survival in patients with resected pancreatic adenocarcinoma

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World Journal of Gastrointestinal Oncology 2012年第10期4卷 207-215页

作者： Sabrina C Wentz Zhi-Guo Zhao Yu Shyr Chan-Juan Shi Kay Washington Nipun B Merchant Fen Xia A Bapsi Chakravarthy Department of Pathology The Johns Hopkins HospitalBaltimoreMD 21201United States Department of Biostatistics Vanderbilt University Medical CenterNashvilleTN 37215United States Department of Pathology Vanderbilt University Medical CenterNashvilleTN 37215United States Department of Surgery Vanderbilt University Medical CenterNashvilleTN 37215United States Department of Radiation Oncology Vanderbilt University Medical CenterNashvilleTN 37215United States

AIM:Clinicopathologic factors predicting overall survival (OS) would help identify a subset to benefit from adjuvant therapy. METHODS: One hundred and sixty-nine patients patients from 1984 to 2009 with curative resections for pancreatic adenocarcinoma were included. Tumors were staged by American Joint Committee on Cancer 7th edition criteria. Univariate and multivariable analyses were performed using Kaplan-Meier methodology or Cox proportional hazard models. Log-rank tests were performed. Statistical inferences were assessed by two-sided 5% significance level. RESULTS: Median age was 67.1 (57.2-73.0) years with equal gender distribution. Tumors were in the head (89.3%) or body/tail (10.7%). On univariate analysis, adjuvant therapy, lymph node (LN) ratio, histologic grade, negative margin status, absence of peripancreatic extension, and T stage were associated with improved OS. Adjuvant therapy, LN ratio, histologic grade, number of nodes examined, negative LN status, and absence of peripancreatic extension were associated with improved recurrence-free survival (RFS). On multivariable analysis, LN ratio and carbohydrate antigen (CA) 19-9 levels were associated with OS. LN ratio was associated with RFS. CONCLUSION: The LN ratio and CA 19-9 levels are independent prognostic factors following curative resections of pancreatic cancer.

关键词： Pancreatic adenocarcinoma Lymph node ratio Carbohydrate antigen 19-9 Recurrence-free survival Overall survival

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Tectonic Evolution and Dynamics of Deepwater Area of Pearl River Mouth Basin, Northern South China Sea

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Journal of China university of Geosciences 2009年第1期20卷 147-159页

作者：董冬冬张功成钟锴袁圣强吴时国 Key Laboratory of Marine Geology and Environment Institute of Oceanology Chinese Academy of SciencesQingdao 266071 China CAS Key Laboratory of Marginal Sea Geology South China Sea Institute of Oceanology Chinese Academy of SciencesGuangzhou 510301 China CNOOC Research center Beijng 100027 China graduate university of Chinese Academy of Sciences Beijing 100049 China College of Georesource and Information China University of PetroleumQingdao 266555 China

Quantitative studies on the evolution and dynamics of the deepwater area of Pearl River Mouth basin （PRMB） were carried out based on the latest geological and seismic data. The study area is generally in an extensional state during the Cenozoic. The major extension happened in the earlier syn-rift stages before 23 Ma and the extension after 23 Ma is negligible. Two rapid subsidence periods, 32-23 Ma and 5.3-2.6 Ma, are identified, which are related to the abrupt heat decay during margin breakup and the collision between the Philippine Sea plate and the Eurasian plate, respectively. The strongest crustal thinning in the Baiyun （白云） sag may trigger the syn-rift volcanism along the weak faulted belt around the sag. The Cenozoic tectonic evolution of the study area could be divided into five stages： rifting （～50-40 Ma）, rift-drift transition （～40-32 Ma）, early post-breakup （～32-23 Ma）, thermal subsidence （～23-5.3 Ma） and neotectonic movement （～5.3-0 Ma）.

关键词： Pearl River Mouth basin South China Sea tectonic evolution dynamic mechanism stretching factor.

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Forming of magnesium alloy by underwater shock wave

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Journal of Magnesium and Alloys 2016年第1期4卷 27-29页

作者： Liqun Ruan Shunsuke Ezaki Fujita Masahiro Shirley Shen Yosihito Kawamura Kumamoto university Magnesium Research center(MRC) Kurokami 2-39-1KumamotoPostcode 860-8555 Japan Department of Mechanical Systems Engineering graduate School of Science and Technology Kumamoto UniversityKurokami 2-39-1Kumamoto-shi Postcode 860-8555Japan Soujyou university Kumamoto-shiJapan CSIRO Manufacturing Flagship Private Bag 10Clayton South 3149Australia

Magnesium alloys are best known for their light weight and high strength-to-weight *** the forging process of magnesium alloys,hot working of has been often carried ***,the strength of the material is reduced because the material temperature reaches the recrystallization temperature during hot ***,the cold method working is required to maintain or increase the strength of a *** this study,we have designed experimental equipment to examine whether the forming of magnesium alloy is possible in the cold working by utilizing underwater shock wave,and the hardness of the material changes by receiving the underwater shock *** results revealed increase in coefficient of extension and hardness of materials after receiving a shock wave.

关键词： Magnesium alloy Cold working Shock wave Forging Coefficient of extension Vickers hardness

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Measuring the thermal conductivity and interfacial thermal resistance of suspended MoS2 using electron beam self-heating technique

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Science Bulletin 2018年第7期63卷 452-458页

作者： Adili Aiyiti Xue Bai Jing Wu Xiangfan Xu Baowen Li center for Phononics and Thermal Energy Science School of Physics Science and Engineering Tongji University Shanghai 200092 China China-EU Joint centerfor Nanophononics School of Physics Science and Engineering Tong ji University Shanghai 200092 China Shanghai Key Laboratory of Special Artificial Microstructure Materials and Technology School of Physics Science and Engineering Tongji University Shanghai 200092 China NUS graduate School for Integrative Sciences and Engineering National University of Singapore Kent Ridge 119620 Singapore Institute of Materials Research and Engineering (IMRE) 2 Fusionopolis Way Innovis #08-03 138634 Singapore Department of Mechanical Engineering University of Colorado Boulder CO 80309-0427 USA

Establishment of a new technique or extension of an existing technique for thermal and thermoelectric measurements to a more challenging system is an important task to explore the thermal and thermoelectric properties of various materials and systems. The bottleneck lies in the challenges in measuring the thermal contact resistance. In this work, we applied electron beam self-heating technique to derive the intrinsic thermal conductivity of suspended Molybdenum Disulfide （MoS2） ribbons and the thermal contact resistance, with which the interracial thermal resistance between few-layer MoS2 and Pt electrodes was calculated. The measured room temperature thermal conductivity of MoS2 is around -30 W/（m K）, while the estimated interracial thermal resistance is around -2 × 10 -6 m-2 K/W. Our experiments extend a useful branch in application of this technique for studying thermal properties of suspended layered ribbons and have potential application in investigating the interracial thermal resistance of different twodimensional （2D） heterojunctions.

关键词： MoS2 Thermal conductivity Thermal resistance E-Beam self-heating technique

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Simulation of rice paddy systems in SWAT:A review of previous applications and proposed SWAT+rice paddy module

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International Journal of Agricultural and Biological Engineering 2022年第1期15卷 1-24页

作者： Philip W.Gassman Jaehak Jeong Julien Boulange Balaji Narasimhan Tasuku Kato Hiroaki Somura Hirozumi Watanabe Sadao Eguchi Yuanlai Cui Atsushi Sakaguchi Le Hoang Tu Rui Jiang Min-Kyeong Kim Jeffrey G.Arnold Wei Ouyang center for Agricultural and Rural Development Iowa State UniversityAmesIowa 50011-1070USA Blackland Research&extension center Texas A&M AgriLifeTempleTexas 76502USA Department of International Environmental and Agricultural Sciences Tokyo University of Agriculture and TechnologyFuchuTokyo 183-8509Japan Department of Civil Engineering Indian Institute of Technology MadrasChennai 600036India graduate School of Environmental and Life Science Faculty of EngineeringOkayama UniversityOkayama 700-8530Japan Institute for Agro-Environmental Sciences National Agriculture and Food Research OrganizationTsukuba 305-8604Japan State Key Laboratory of Water Resources and Hydropower Engineering Science Wuhan UniversityWuhan 430072China Department of Biological&Environmental Sciences Faculty of AgricultureYamaguchi UniversityYamaguchi 753-8515Japan Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China Ministry of AgricultureCollege of Natural Resources and EnvironmentNorthwest A&F UniversityYangling 712100ShaanxiChina Rural Development Administration Jeonju54875Republic of Korea Grassland Soil and Water Research Laboratory U.S.Department of Agriculture-Agricultural Research ServiceTempleTexas 76502USA School of Environment State Key Laboratory of Water Environment SimulationBeijing Normal UniversityBeijing 100875China

The Soil and Water Assessment Tool(SWAT)is an ecohydrological watershed-scale model which was initially developed in the early 1990s to simulate the impacts of land use,management systems,and climate on hydrology and/or water *** adopted in the U.S.,the use of the model then spread to Europe and then later to Asia and other *** range of applications that SWAT has been applied to have also expanded dramatically,which influenced ongoing model development which has been virtually continuous over the past two decades.A key component of many SWAT applications in Asia is accounting for rice paddy production that is common in some subregions within the ***,most of these studies do not provide explicit details of how rice production was simulated in *** research has revealed that significant problems occur when trying to represent rice paddy systems in standard versions of SWAT,due to limitations in algorithms based on the runoff curve number approach or the pothole *** response,key modifications have been made to SWAT in recent studies that have resulted in more accurate representation of rice paddy *** developments point to the need for the incorporation of an enhanced rice paddy module within SWAT to better capture rice paddy hydrological and pollutant dynamics,which would support improved use of the model in Asia and other rice production *** related to simulating rice production in SWAT are discussed as follows:1)an overview of global rice production;2)history of SWAT development;3)typical approaches for simulating rice production;4)problems associated with the typical approaches;5)recent code modifications to address deficiencies in replicating rice paddy systems;6)recommendations for developing a standard rice paddy module for future SWAT codes.

关键词： SWAT rice paddies potholes hydrology pollutants modified SWAT models

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Long-term effects of silver nanoparticles on performance of phosphorus removal in a laboratory-scale vertical flow constructed wetland

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Journal of Environmental Sciences 2020年第1期32卷 319-330页

作者： Juan Huang Jun Xiao Yang Guo Wenzu Guan Chong Cao Chunni Yan Mingyu Wang School of Civil Engineering Southeast UniversityNanjing 211189China Security Support center for Urban Water Supply of Jiangsu Province Nanjing 210036China

Silver nanoparticles(AgNPs)have been widely used in many fields,which raised concerns about potential threats to biological sewage treatment *** this study,the phosphorus removal performance,enzymatic activity and microbial population dynamics in constructed wetlands(CWs)were evaluated under a long-term exposure to Ag NPs(0,50,and 200μg/L)for 450 *** have shown that Ag NPs inhibited the phosphorus removal efficiency in a short-term exposure,whereas caused no obviously negative effects from a long-term ***,in the coexisting CW system of Ag NPs and phosphorus,competition exhibited in the initial exposure phase,however,cooperation between them was observed in later *** activity of acid-phosphatase at the moderate temperature(10–20℃)was visibly higher than that at the high temperature(20–30℃)and CWs with Ag NPs addition had no appreciable differences compared with the ***-throughput sequencing results indicated that the microbial richness,diversity and composition of CWs were distinctly affected with the extension of exposure time at different Ag NPs ***,the phosphorus removal performance of CWs did not decline with the decrease of polyphosphate accumulating organisms(PAOs),which also confirmed that adsorption precipitation was the main way of phosphorus removal in *** study suggested that Ag NPs and phosphorus could be removed synergistically in the coexistence *** work has some reference for evaluating the influences of Ag NPs on the phosphorus removal and the interrelation between them in CWs.

关键词： Silver nanoparticles(AgNPs) Phosphorus removal Constructed wetlands Enzymatic activity Microbial community

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Indigenous Purple Vegetable Extracts Protect against Hydrogen Peroxide-Induced DNA Damage in Human Lymphocytes

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Food and Nutrition Sciences 2013年第8期4卷 62-70页

作者： Pi-Yu Chao Wei-Yu Huang Shene-Pin Hu Hsiao-Feng Lo Kuan-Hung Lin Meng-Yuan Huang Tsan-Ru Chang Chi-Ming Yang Department of Food Health and Nutrition Science Chinese Culture University Taipei Taiwan Department of Horticulture and Landscape Architecture National Taiwan University Taipei Taiwan graduate Institute of Bio- technology Chinese Culture University Taipei Taiwan Institute of Applied Life Science Chinese Culture University Taipei Taiwan Research center for Biodiversity Academia Sinica Taipei Taiwan School of Nutrition and Health Sciences Taipei Medical University Taipei Taiwan Taoyuan District Agricultural Research and extension Station Council of Agriculture Executive Yuan Taoyuan Taiwan

The objectives of this study were to identify the antioxidants in acid hydrolysates of six of Taiwan’s indigenous purple-leaved vegetables (IPLV), including purple-leaved sweet potato (Ipomoea batatas L. Lamark), purple-leaved gynura line 1 (Gynura bicolor D.C.), purple-leaved gynura line 2 (Gynura bicolor D.C.), purple-leaved perilla line 1 (Perilla frutescents L. Britton), bicolored-leaved perilla line 2 (Perilla frutescens L. Britton), and heartleaf houttuynia (Houttuynia cordata Thumb.). Their antioxidant activity and ability to protect DNA from oxidative damage was also evaluated. The antioxidants identified included flavonoids, anthocyanidins and flavonols. Both purple-leaved perilla line 1 and bicolored-leaved perilla line 2 were found to be abundant in cynidin, malvidin, and myricetin. Moreover, purple-leaved sweet potato and heartleaf houttuynia were found to be abundant in quercetin. Purple-leaved sweet potato and purple-leaved perilla line 1 contained significantly higher inhibition percentages of conjugated diene formation than other vegetables. Purple-leaved sweet potato, heartleaf houttuynia, purple-leaved perilla line 1, and bicolored-leaved perilla line 2 had better inhibition percentages of tail DNA% and tail moment in the study. In conclusion, the abovementioned four indigenous purple-leaved vegetables abounded in antioxidants had strong antioxidant activity for protecting DNA in lymphocytes from oxidative damages.

关键词： Antioxidant Indigenous Purple-Leaved Vegetables Comet Assay Flavonols Cynidin

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Functional Analysis of Semi-conserved Transit Peptide Motifs and Mechanistic Implications in Precursor Targeting and Recognition

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Molecular Plant 2016年第9期9卷 1286-1301页

作者： Kristen Holbrook Chitra Subramanian Prakitchai Chotewutmontri L. Evan Reddick Sarah Wright Huixia Zhang Lily Moncrief Barry D. Bruce Department of Biochemistry Cellular and Molecular Biology graduate Program in Plant Physiology and Genetics graduate School of Genome Science and Technology Department of Botany Department of Botanyuniversity of Tennessee Knoxville TN 37996 USA Present address: Department of Infectious Disease St. Jude Children's Research Hospital Memphis TN 38105 USA Present address: Institute of Molecular Biology University of Oregon Eugene OR 97403 USA present address: Department of Microbiology University of Texas Southwestern Medical Center 5323 Harry Hines Boulevard Dallas TX 75390 USA Present address: Albert R. Mann Library Cornell University Ithaca NY 14850 USA present address: U.S. Food and Drug Administration 10903 New Hampshire Avenue Silver Spring MD 20993 USA

Over 95% of plastid proteins are nuclear-encoded as their precursors containing an N-terminal extension known as the transit peptide （TP）. Although highly variable, TPs direct the precursors through a conserved, posttranslational mechanism involving translocons in the outer （TOC） and inner envelope （TOC）. The organelle import specificity is mediated by one or more components of the Toc complex. However, the high TP diversity creates a paradox on how the sequences can be specifically recognized. An emerging model of TP design is that they contain multiple loosely conserved motifs that are recognized at different steps in the targeting and transport process. Bioinformatics has demonstrated that many TPs contain semiconserved physicochemical motifs, termed FGLK. In order to characterize FGLK motifs in TP recognition and import, we have analyzed two well-studied TPs from the precursor of RuBisCO small subunit （SStp） and ferredoxin （Fdtp）. Both SStp and Fdtp contain two FGLK motifs. Analysis of large set mutations （-85） in these two motifs using in vitro, in organello, and in vivo approaches support a model in which the FGLK domains mediate interaction with TOC34 and possibly other TOC components. In vivo import analysis suggests that multiple FGLK motifs are functionally redundant. Furthermore, we discuss how FGLK motifs are required for efficient precursor protein import and how these elements may permit a convergent function of this highly variable class of targeting sequences.

关键词： chloroplast biology protein translocation Toc34 transit peptide cell biology

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