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Inhibitory gamma-aminobutyric acidergic neurons in the anterior cingulate cortex participate in the comorbidity of pain and emotion

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Neural Regeneration Research 2025年第10期20卷 2838-2854页

作者： Lu Guan Mengting Qiu Na Li Zhengxiang Zhou Ru Ye Liyan Zhong Yashuang Xu Junhui Ren Yi Liang Xiaomei Shao Jianqiao Fang Junfan Fang Junying Du Department of Neurobiology and Acupuncture Research Third School of Clinical MedicineZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Key Laboratory for Research of Acupuncture Treatment and Transformation of Emotional Diseases Third School of Clinical MedicineZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Fuchun Community Health service center of Fuyang district HangzhouZhejiang ProvinceChina

Pain is often comorbid with emotional disorders such as anxiety and *** of the anterior cingulate cortex has been implicated in pain and pain-related negative emotions that arise from impairments in inhibitory gamma-aminobutyric acid *** review primarily aims to outline the main circuitry(including the input and output connectivity)of the anterior cingulate cortex and classification and functions of different gamma-aminobutyric acidergic neurons;it also describes the neurotransmitters/neuromodulators affecting these neurons,their intercommunication with other neurons,and their importance in mental comorbidities associated with chronic pain *** understanding on their role in pain-related mental comorbidities may facilitate the development of more effective treatments for these ***,the mechanisms that regulate gamma-aminobutyric acidergic systems remain *** is also unclear as to whether the mechanisms are presynaptic or *** exploration of the complexities of this system may reveal new pathways for research and drug development.

关键词： anterior cingulate cortex anxiety chronic pain circuit communication comorbidity depression gamma-aminobutyric acidergic neurons parvalbumin neurons synaptic transmission

Characteristics of Central Heating Cinder and Its Influence on Soil Properties

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Meteorological and Environmental Research 2022年第2期13卷 78-82,97页

作者： Guoyu GAN Chao LI Xu ZHANG Li YANG Yunfeng CHEN Bo LIU Yanli LI Jifu LI College of Agriculture/Analytical&Testing center of agricultural Resources and Environment Yangtze UniversityJingzhou 434025China jingzhou district agricultural science and technology service center Jingzhou 434025China Key Laboratory of Fertilization Utilization of Waste Ministry of Agriculture and Rural AreasWuhan 430070China

[Objective]The research was designed to explore the effect of long-term stacking of solid waste produced in the coal-burning process of central heating enterprises on physical and chemical properties of soil.[Method]This study took the heating enterprises in Shenyang City as the research *** morphological structure and element composition of coal cinder were determined by continuously collecting coal and cinder samples in different *** the same time,the original soil and cinder soil of the stacking site were collected to determine the changes of soil morphological structure,element composition and physical and chemical properties,so as to provide reference for the resource utilization of local cinder waste and the potential pollution risk of the stacking site.[Result]The contents of C,H,O,N,and S non-metallic elements in coal cinder decreased by 69.5%,71.2%,76.0%,74.5%,and 34.6%,respectively when compared with raw coal;while the content of Si increased significantly by 95.7%.The contents of Al,K,and Fe in cinder decreased by 4.3%,60.2%,and 33.3%,respectively,while the contents of Mg and Na increased by 36.1%and 130.9%,*** with the original soil,the contents of C,H,and O in shallow cinder soil and deep cinder soil increased by 126.5%,67.9%,80.93%,and 21.3%,25.0%and 42.3%,*** residual carbon existed in the form of activated *** contents of Mg,Al,K,Na,Ca,and Fe in shallow cinder soil and deep cinder soil increased by 61.6%,5.4%,46.1%,35.8%,32.5%,6.3%and 22.3%,12.3%,12.2%,15.6%,5.8%and 2.8%,respectively compared with the original *** content of heavy metal elements in coal cinder did not reach the detection *** the scanning electron microscope,the raw coal is mainly block structure,while the cinder is honeycombed and porous and dust.[Conclusion]Cinder stacking can significantly improve the content of organic matter and available K in shallow cinder soil,and improve the porosity and permeability of ***

关键词： Central heating Coal cinder Soil properties Element composition Morphology and structure

Comparison of Three Different Techniques of Human Sperm DNA Isolation for Methylation Assay

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Journal of Huazhong University of science and technology(Medical sciences) 2015年第6期35卷 938-942页

作者：袁红方马丁苏莉杨帆扈智勇田浡臻章慧平赵凯 center of Reproductive Medicine Institute of Family Planning Research Tongji Medical College Huazhong University of Science and Technology Family Planning Women and Children service center of Zeng Du district

Summary： Human sperm DNA is an important genetic and epigenetic material, whose chromatin structure differs from that of somatic cells. As such, conventional methods for DNA extraction of somatic cells may not be suitable for obtaining sperm DNA. In this study, we evaluated and compared three sperm DNA extraction techniques, namely, modified guanidinium thiocyanate method （method A）, traditional phenol-chloroform method （method B）, and TianGen kit method （method C）. Spectrophotometry and agarose gel electrophoresis analyses showed that method A produced DNA with higher quantity and purity than those of methods B and C （P〈0.01）. PCR results revealed that method A was more reliable in amplifying DEAD-box polypeptide 4 （DDX4） and copy number variations （CNVs） than methods B and C, which generated false-positive errors. The results of sperm DNA methylation assay further indicated that methods A and B were effective, and the former yielded higher quantitative accuracy. In conclusion, the modified guanidinium thiocyanate method provided high quality and reli- able results and could be an optimal technique for extracting sperm DNA for methylation assay.

关键词： human sperm DNA isolation modified guanidinium thiocyanate phenol-chloroform TianGen kit

Drip fertigation and plant hedgerows significantly reduce nitrogen and phosphorus losses and maintain high fruit yields in intensive orchards

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Journal of Integrative Agriculture 2023年第2期22卷 598-610页

作者： SONG Ke QIN Qin YANG Ye-feng SUN Li-juan SUN Ya-fei ZHENG Xian-qing Lü Wei-guang XUE Yong Eco-Environmental Protection Research Institute Shanghai Academy of Agricultural SciencesShanghai 201403P.R.China Pudong New district Agro-technology Extension service center Shanghai 201300P.R.China

A field experiment was carried out to evaluate the effects of drip fertigation combined with plant hedgerows on nitrogen and phosphorus runoff losses in intensive pear orchards in the Tai Lake *** and phosphorus runoff over a whole year were measured by using successional runoff water collection *** four experimental treatments were conventional fertilization(CK),drip fertigation(DF),conventional fertilization combined with plant hedgerows(C+H),and drip fertigation combined with plant hedgerows(D+H).The results from one year of continuous monitoring showed a significant positive correlation between precipitation and surface runoff *** runoff discharge under the treatments without plant hedgerows totaled 15.86%of precipitation,while surface runoff discharge under the treatments with plant hedgerows totaled 12.82%of *** hedgerows reduced the number of runoff events and the amount of surface *** is the main driving force for the loss of nitrogen and phosphorus in surface runoff,and fertilization is an important factor affecting the losses of nitrogen and *** CK,approximately 7.36%of nitrogen and 2.63%of phosphorus from fertilization entered the surface water through *** fertigation reduced the accumulation of nitrogen and phosphorus in the surface soil and lowered the runoff loss concentrations of total nitrogen(TN)and total phosphorus(TP).Drip fertigation combined with plant hedgerows significantly reduced the overall TN and TP losses by 45.38 and 36.81%,respectively,in comparison to the CK *** fertigation increased the vertical migration depth of nitrogen and phosphorus nutrients and reduced the accumulation of nitrogen and phosphorus in the surface soil,which increased the pear *** promotion of drip fertigation combined with plant hedgerows will greatly reduce the losses of nitrogen and phosphorus to runoff and maintain the high fruit yields in the intensive orchards of

关键词： drip fertigation plant hedgerows surface runoff nitrogen and phosphorus losses fruit yields

Comparison of sleep timing of people with different chronotypes affected by modern lifestyle

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Chinese Physics B 2023年第6期32卷 682-688页

作者：李莹郭纪辰王雪 College of Information technology Shanghai Ocean UniversityShanghai 201306China Hangtou Hesha Community Health service center Pudong New DistrictShanghai 201317China

Circadian rhythm is an endogenous rhythmic behavior of organisms which can be entrained by daily light–dark *** timing of human sleep-cycle is regulated by endogenous circadian rhythm and homeostatic processes. Light exposure affects both sleep timing and circadian rhythm. Now humans can extend lighting time by turning on artificial lights and wake up time is usually triggered by alarm clocks to meet social schedules. This modern lifestyle is believed to be related with a temporal mismatch between sleep and circadian rhythmicity(social jet-lag) and insufficient sleep, which lead to ill mental and physical health outcomes. At present, the impacts of self-selection of light exposure and social constrains on sleep timing is far from clear. According to preferred sleep-wake schedule, there are three different chronotypes. In this paper, we apply a mathematical model to get a quantitative comparison of sleep timing of people with different chronotypes with the effects of modern light consumption and social constrains. The results show that the prolonged day light and evening light exposure both delay preferred sleep timing with the sleep duration almost unchanged. People of evening-type or with longer intrinsic periods are most expected to be vulnerable to evening light. Increasing light exposure can offset the effect of evening light to some extent, but it is most difficult for evening-type people. Social constrains cause the largest social jet-lag in people of evening-type, which increases with evening light intensity or intrinsic periods. Morning-type people's sleep symptoms worsens, while that of evening-type people improves with age. This study provides a theoretical reference for preventing and treating sleep disorder and social jet-lag for individuals with different chronotypes.

关键词： chronotype self-selected light sleep social constraint

Numerical simulation of compaction parameters for sand-filled embankment using large thickness sand-filling technique in Jianghan Plain district

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Frontiers of Structural and Civil Engineering 2018年第4期12卷 568-576页

作者： Wentao WANG Chongzhi TU Rong LUO National center for Materials service Safety University of Science and Technology BeijingBeijing 100083China Sehool of Transportation Wuhan University of TechnologyWuhan 430063China

The study uses the finite element method to simulate a new technique of highway sand embankment filling in Jianghan Plain district, which can raise the thickness of sand-filled layer from 30 cm to 70 cm and can significantly shorten the construction period based on the guarantee of sand embankment construction quality. After simulating the three compacting proposals carried out on the field test, the study uses COMSOL software to research on the compacting effects of sand-filled layers in larger thicknesses by 22 ton vibratory roller alone, and then to investigate the steady compacting effect of 12 ton vibratory roller. The simulation results indicate that the sand-filled layer thickness of 70 cm is suitable for the new sand filling technique, and the sand-filled embankment project with tight construction period is suggested to choose the 12 ton vibration roller for steady compaction.

关键词： sand embankment compaction in large thickness numerical simulation small size vibratory roller steady compaction

Clinical Observation on Treatment of Cervical Spondylotic Radiculopathy with Combined Electroacupuncture Tuina and Traction

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Journal of Acupuncture and Tuina science 2009年第3期7卷 156-158页

作者：华宇韩丑萍 Huajing Community Health service center Xuhui District

Objective： To observe the clinical effect of combined electroacupuncture, tuina. and traction treating cervical spondylotic radiculopathy. Method： Depending on the orders of the patient＇s first visit, 120 cases were randomized into a treatment group （60 cases）, receiving electroacupuncture, tuina and traction treatment, and a control group （60 cases）, receiving electroacupuncture and tuina treatment. The treatment was done once a day, and 5 times constitute one course of treatment. A 2-day interval is set between the courses. The therapeutic effect was evaluated after 6 courses of treatment. Result： The total effective rate and recovery rate in the treatment group were 93.3% and 61.7%, respectively, whereas that of the control group were 78.3% and 31.7%, respectively, showing a significant statistical difference. Conclusion： Combined acupuncture, tuina and traction worked well for cervical spondylotic radiculopathy.

关键词： Spondylosis Neck pain Electroacupuncture Tuina Massage Traction

Development of a high-efficiency sex pheromone formula to control Euproctis pseudoconspersa

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Journal of Integrative Agriculture 2023年第1期22卷 195-201页

作者： LI Zhao-qun YUAN Ting-ting CUI Shao-wei ZHAO Ying-jie SHAO Yuan-hai SHANG Jian-nong LUO Zong-xiu CAI Xiao-ming BIAN Lei CHEN Zong-mao Key Laboratory of Biology Genetics and Breeding of Special Economic Animals and Plants of Ministry of Agriculture and Rural AffairsTea Research InstituteChinese Academy of Agricultural ScienceHangzhou 310008P.R.China Tea Variety Research Institute of Wuxi Wuxi 214122P.R.China agricultural technology Extension service center of Xihu district of Hangzhou Hangzhou 310063P.R.China

The tea tussock moth(Euproctis pseudoconspersa)is one of the most destructive chewing pests in tea plantations and causes a serious allergic reaction on the skin of tea plantation *** sex pheromone components of its Japanese population were first identified as 10,14-dimethylpentadecyl isobutyrate(10Me14Me-15:iBu)and 14-methylpentadecyl isobutyrate(14Me-15:iBu),with a stereogenic *** 10Me14Me-15:iBu has been identified in the Chinese *** ***,field tests have shown that 10Me14Me-15:iBu cannot meet the demand of effective pest control in *** develop a high-efficiency *** sex pheromone formula,electroantennogram(EAG)recordings of(S)-and(R)-enantiomers of 10Me14Me-15:iBu and 14Me-15:iBu were obtained in the present *** results demonstrated that the EAG responses of male antennae to(R)-enantiomers were significantly higher than responses to the(S)-enantiomers,and 14Me-15:iBu also elicited EAG *** tests showed that the catch numbers of male moths by(R)-enantiomers were significantly higher(P<0.05)than those of(S)-*** of 14Me-15:iBu significantly increased the catch numbers of both the(S)-and(R)-*** efficient pheromone formula containing 0.75 mg(R)-10Me14Me-15:iBu and 0.1 mg 14Me-15:iBu showed significantly higher attractiveness than commercial pheromone *** study demonstrated that(R)-10Me14Me-15:iBu was the major sex pheromone component of ***,and 14Me-15:iBu might be the minor sex pheromone ***,a high-efficiency sex pheromone formula for *** control was defined in this study.

关键词： Euproctis pseudoconspersa sex pheromone chirality electroantennogram

Study on burrowing nematode, Radopholus similis, pathogenicity test system in tobacco as host

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Journal of Integrative Agriculture 2022年第9期21卷 2652-2664页

作者： YANG Si-hua ZHAO Li-rong DING Sha TANG Shi-qiao CHEN Chun ZHANG Huan-xin XU Chun-ling XIE Hui Research center of Nematodes of Plant Quarantine Laboratory of Plant NematologyCollege of Plant ProtectionSouth China Agricultural UniversityGuangzhou 510642P.R.China technology center Guangzhou Customs DistrictPeople's Republic of ChinaGuangzhou 510623P.R.China Comprehensive technology and service center of Huizhou Port Customs Huizhou 516080P.R.China

Radopholus similis(Cobb 1893) Thorne(1949) is a destructive migratory endoparasitic plant nematode. In this study,the pathogenic process of R. similis infection in Nicotiana benthamiana(tobacco) was studied using quartz sand culture in laboratory. The results showed that R. similis mainly parasitised the root cortex, leading to cortical cell decomposition and tissue decay. We optimised the inoculation conditions to establish a method for determining the pathogenicity of R. similis as follows:(1) a glass culture tube was filled with quartz sand(about 1/3 of the height) and sterilised twice;(2) 20-day-old N. benthamiana seedlings were transplanted into test tubes and cultivated for 10 days at(25±1)°C;(3) R. similis female nematodes were inoculated in the root rhizosphere at a rate of 150 nematodes per plant;(4) the number of nematodes, disease severity, and growth of the plant at 30 days post-inoculation(dpi) were determined. The pathogenicity of eight R. similis populations from different hosts was determined, which proved the feasibility of this method.

关键词： Radopholus similis Nicotiana benthamiana populations pathogenicity