检索结果-南通市图书馆

Gene cloning,protein expression,and enzymatic characterization of a double-stranded RNA degrading enzyme in Apolygus lucorum

引用

Insect Science 2024年第1期31卷 119-133页

作者： Jie-Yu Zhang Jing Zhao Keyan Zhu-Salzman Qin-Qin Ji Yi-Ping Jiang Liu-Bin Xiao De-Jin Xu Guang-Chun Xu Lin-Quan Ge Yong-An Tan College of plant protection Yangzhou UniversityYangzhouJiangsu ProvinceChina Institute of plant protection Jiangsu Academy of Agricultural SciencesNanjingJiangsu ProvinceChina Department of Entomology Texas A&M UniversityCollege StationTXUSA Taizhou Customs of the People's Republic of China TaizhouJiangsu ProvinceChina

RNA interference(RNAi)is a powerful tool that post-transcriptionally silences target genes in eukaryotic cells.However,silencing efficacy varies greatly among different insect species.Recently,we met with little success when attempting to knock down genes in the mirid bug Apolygus lucorum via dsRNA injection.The disappearance of double-stranded RNA(dsRNA)could be a potential factor that restricts RNAi efficiency.Here,we found that dsRNA can be degraded in midgut fluids,and a dsRNase of A.lucorum(AldsRNase)was identified and characterized.Sequence alignment indicated that its 6 key amino acid residues and the Mg2+-binding site were similar to those of other insects’dsRNases.The signal peptide and endonuclease non-specific domain shared high sequence identity with the brown-winged green stinkbug Plautia stali dsRNase.AldsRNase showed high salivary gland and midgut expression and was continuously expressed through the whole life cycle,with peaks at the 4th instar ecdysis in the whole body.The purified AldsRNase protein obtained by heterologously expressed can rapidly degrade dsRNA.When comparing the substrate specificity of AldsRNase,3 specific substrates(dsRNA,small interfering RNA,and dsDNA)were all degraded,and the most efficient degradation is dsRNA.Subsequently,immunofluorescence revealed that AldsRNase was expressed in the cytoplasm of midgut cells.Through cloning and functional study of AldsRNase,the enzyme activity and substrate specificity of the recombinant protein,as well as the subcellular localization of nuclease,the reason for the disappearance of dsRNA was explained,which was useful in improving RNAi efficiency in A.lucorum and related species.

关键词： Apolygus lucorum cloning dsRNase enzymatic activity protein expression

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Minerals in edible insects:a review of content and potential for sustainable sourcing

引用

Food Science and Human Wellness 2024年第1期13卷 65-74页

作者： Mingxing Lu Chenxu Zhu Sergiy Smetana Ming Zhao Haibo Zhang Fang Zhang Yuzhou Du College of plant protection&Institute of Applied Entomology Yangzhou UniversityYangzhou 225009China German Institute of Food Technologies(DIL e.V.) Quakenbrück D-49610Germany Jiangsu plant protection and plant Quarantine station Nanjing 210036China Joint International Research Laboratory of Agriculture and Agri-Product Safety Yangzhou UniversityYangzhou 225009China

In response to the rapid increase in world population and subsequent demands for food,edible insects represent an alternative food source for humans that is rich in proteins,amino acids and minerals.Entomophagy is a tradition in many countries including China and Thailand,and edible insects have attracted a lot of attention in Western World due to their suitable nutrient composition,high mineral content(e.g.,Fe,Zn,Ca,Mg)and potential use as a supplement in human diet.In this study,we surveyed mineral content in seven insect orders and 67 species of mass produced and wild-harvested edible insects.The total content of essential elements in edible insects was very high in Tenebrio molitor,Bombyx mori,and Zonocerus variegatus.The heavy metal content(summarized for eight species)was below the maximum limit allowed for safe consumption.Sustainable supply of minerals derived from insect biomass is complicated due to the high variations of mineral content in insects and the potential of its change due to processing.

关键词： Edible insects Minerals Nutrition Food safety Sustainability

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Acaricidal effect of the antimicrobial metabolite xenocoumacin 1 on spider mite control

引用

Journal of Integrative Agriculture 2024年第3期23卷 948-959页

作者： jiaxing Wei Hong Yan Jie Ren Guangyue Li Bo Zhang Xuenong Xu Institute of plant protection Chinese Academy of Agricultural SciencesBeijing 100193China State Key Laboratory for Biology of plant Diseases and Insect Pests Beijing 100193China Key Laboratory of Natural Enemies Insects Ministry of Agriculture and Rural AffairsBeijing 100193China

The two-spotted spider mite,Tetranychus urticae Koch,is one of the most harmful pests in many agroecosystems worldwide.To effectively manage this pest,there is an urgent need to develop novel bio-active acaricides that support integrated pest management strategies targeting T.urticae.In this study,we explored the acaricidal effects of xenocoumacin 1 (Xcn1) on T.urticae and its predator Neoseiulus californicus using the highly puri?ed ***1 was extracted and purified from the cell-free supernatant of the Xenorhabdus nematophila CB6 mutant constructed by the easy promoter activated compound identi?cation (easyPACId) method.When the concentration of Xcn1 exceeded 100μg mL~(–1),the survival rate of spider mite adults declined to below 40%and the fecundity was decreased by 80%at six days post-application.At concentrations of 25 and 50μg mL~(–1),Xcn1 signi?cantly impeded spider mite development by inhibiting the molt.However,neither concentration had any adverse effects on the survival or reproduction of the predatory mite N.californicus.The results from laboratory and semi-?eld experiments consistently demonstrated the effectiveness of the antimicrobial metabolite Xcn1 in controlling pest mites at both the molecular and physiological levels.Our study offers a promising possibility that combines the compatible biocontrol agents of Xcn1 and predatory mites for integrated pest mite control.

关键词： pest management predatory mite Xcn1 morphology developmental inhibition transcriptome

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Dormancy of Amaranthus retroflexus L. Seeds and Physiological Response Seedlings to Acifluorfen Sodium

引用

Journal of Northeast Agricultural University(English Edition) 2024年第1期31卷 1-10页

作者： Ding Wei Liu Junliang Cheng Zhuo Cheng Peng College of plant protection Northeast Agricultural UniversityHarbin 150030China College of Resources and Environment Northeast Agricultural UniversityHarbin 150030China Agricultural Technology Extension station of Heilongjiang Province Harbin 150010China

Amaranthus retroflexus L. is a serious and widespread malignant weed in soybean fields in Heilongjiang Province. Exploring the dormancy characteristics of A. retroflexus L. seeds and the physiological response of its seedlings to acifluorfen sodium can provide a basis for further researches on its resistance mechanism. Using newly harvested and stored A. retroflexus L. seeds for one year as experimental materials, the effects of different concentrations of HCl, NaOH, water temperature, gibberellic acid(GA) and polyethylene glycol(PEG) on the dormancy and germination of A. retroflexus L. seeds were studied. The sensitivity of A. retroflexus L.to acifluorfen sodium was determined using bioassay. The effects on leaf chlorophyll content and target enzyme activity were studied at a normal dosage of 360 g a.i. hm^(-2) and a doubling dosage of 720 g a.i. hm^(-2) of acifluorfen sodium. Newly harvested seeds exhibiting dormancy were soaked in water of various temperatures and in different concentrations of NaOH and HCl, which were ineffective in breaking the seed dormancy. GA could break seed dormancy, and the highest seed germination rate reached 93.33% when they were soaked at 3 000 mg·L^(-1) for 72 h and 4 000 mg·L^(-1) for 48 h. The drought stress was simulated with a 15%-25% polyethylene glycol solution, which had no significant effect on the seed germination rate. The GR_(50) value of acifluorfen sodium for A. retroflexus L. was 705.7 g a.i. hm^(-2), which was 1.96 times the recommended dose in the field. After the application of different doses of acifluorfen sodium, the chlorophyll content of A. retroflexus L. reached its minimum value 3 days after treatment(DAT), and then gradually increased. The activity of the target enzyme protoporphyrinogen oxidase(PPO) reached the highest value at 7 DAT under different dosages, and gradually returned to normal levels thereafter. Soaking with gibberellin was an effective method to break seed dormancy. A. retroflexus L. seeds had certain drought resistance during the germinati

关键词： Amaranthus retroflexus L.seed seed dormancy acifluorfen sodium target resistance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Different responses of soil fauna gut and plant rhizosphere microbiomes to manure applications

引用

Soil Ecology Letters 2024年第2期6卷 57-69页

作者： Shuai Du Xiang Zhao Yue Zhang Changlong Shu Ju-Pei Shen Key Laboratory of Urban Environment and Health Ningbo Urban Environment Observation and Research StationInstitute of Urban EnvironmentChinese Academy of SciencesXiamen361021China School of Geographical Sciences Fujian Normal UniversityFuzhou350007China Institute of plant Nutrition Resources and EnvironmentBeijing Academy of Agriculture and Forestry SciencesBeijing100097China State Key Laboratory for Biology of plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing100193China Hebei Key Laboratory of Soil Entomology Cangzhou Academy of Agricultural and Forestry SciencesCangzhou061001China

Microbial attributes were compared between soil fauna gut and plant rhizosphere.•Manure applications decreased or increased gut or rhizosphere bacterial diversity.•Stochastic or deterministic processes drove gut or rhizosphere bacterial assembly.•Manure applications increased bacterial network complexity of gut and rhizosphere.Diverse microbes inhabit animals and plants,helping their hosts perform multiple functions in agricultural ecosystems.However,the responses of soil fauna gut and plant rhizosphere microbiomes to livestock manure applications are still not well understood.Here we fed Protaetia brevitarsis larvae(PBL)with chicken manure and collected their frass.The frass and manure were applied as fertilizers to lettuce pots.We then compared the changes of microbial diversity,community assembly,and potential functions between the gut group(i.e.,all PBL gut and frass samples)and the rhizosphere group(i.e.,all rhizosphere soil samples).We revealed that manure applications(i.e.,feeding or fertilization)decreased bacterial diversity in the gut group but increased that in the rhizosphere group.Particularly,the proportions of Bacilli in the gut group and Gammaproteobacteria in the rhizosphere group were increased(up to a maximum of 33.8%and 20.4%,respectively)after manure applications.Stochastic and deterministic processes dominated community assembly in the gut and rhizosphere microbiomes,respectively.Manure applications increased the microbial co-occurrence network complexity of both the gut and rhizosphere groups.Moreover,the proportions of functional taxa associated with human/animal pathogens in the gut group and carbon/nitrogen cycling in the rhizosphere group were enhanced(up to 2.6-fold and 24.6-fold,respectively).Our findings illustrate the different responses of microbial diversity,community assembly,and potential functions in soil fauna gut and plant rhizosphere to manure applications.The results could enhance our knowledge on the reasonable utilization of animal and plant microbiomes in agricultural ma

关键词： soil fauna gut plant rhizosphere manure applications community assembly microbial functions

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

AcWRKY28 mediated activation of AcCPK genes confers salt tolerance in pineapple(Ananas comosus)

引用

Horticultural plant Journal 2024年第2期10卷 398-412页

作者： Qiao Zhou Samaranayaka Vidana Gamage Nirosha Priyadarshani Rongjuan Qin Han Cheng Tiantian Luo Myat Hnin Wai Mohammad Aqa Mohammadi Yanhui Liu Chang Liu Hanyang Cai Xiaomei Wang Yeqiang Liu Yuan Qin Lulu Wang Guangxi Key Lab of Sugarcane Biology State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesCollege of AgricultureGuangxi UniversityNanningGuangxi 530004China State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops College of Life ScienceCollege of Plant ProtectionCollege of AgricultureCollege of HorticultureFujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyCenter for Genomics and BiotechnologyFujian Agriculture and Forestry UniversityFuzhouFujian 350002China Pingtan Science and Technology Research Institute of Fujian Agriculture and Forestry University PingtanFujian 350400China National Education Commission BMICHColombo00700Sri Lanka Fishery Multiplication Management station of Lijiang River Water Supply Hub Project GuilinGuangxi 541001China Horticulture Research Institute Guangxi Academy of Agricultural SciencesNanning Investigation Station of South Subtropical Fruit TreesMinistry of AgricultureNanningGuangxi 530007China

Unfavorable environmental cues severely affect crop productivity resulting in significant economic losses to farmers. In plants, multiple regulatory genes, such as the WRKY transcription factor (TF) family, modulate the expression of defense genes. However, the role of the pineapple WRKY genes is poorly understood. Here, we studied the pineapple WRKY gene, AcWRKY28, by generating AcWRKY28 over-expressing transgenic pineapple plants. Overexpression of AcWRKY28 enhanced the salt stress resistance in transgenic pineapple lines. Comparative transcriptome analysis of transgenic and wild-type pineapple plants showed that “plant-pathogen interaction” pathway genes, including 9calcium-dependent protein kinases (CPKs), were up-regulated in AcWRKY28 over-expressing plants. Furthermore, chromatin immunoprecipitation and yeast one-hybrid assays revealed AcCPK12, AcCPK3, AcCPK8, AcCPK1, and AcCPK15 as direct targets of AcWRKY28. Consistently, the study of AcCPK12 over-expressing Arabidopsis lines showed that AcCPK12 enhances salt, drought, and disease resistance. This study shows that AcWRKY28 plays a crucial role in promoting salt stress resistance by activating the expression of AcCPK genes.

关键词： Pineapple AcWRKY AcCPK Transcription factor Salt stress ChIP

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The storage and utilization of carbohydrates in response to elevation mediated by tree organs in subtropical evergreen broad-leaved forests

引用

Forest Ecosystems 2024年第1期11卷 52-61页

作者： Bin Xu Xueli Jiang Yingying Zong G.Geoff Wang Fusheng Chen Zhenyu Zhao Xiangmin Fang Key Laboratory of National Forestry and Grassland Administration on Forest Ecosystem protection and Restoration of Poyang Lake Watershed College of ForestryJiangxi Agricultural UniversityNanchang330045China Jiulianshan National Observation and Research station of Chinese Forest Ecosystem College of ForestryJiangxi Agricultural UniversityNanchang330045China Department of Forestry and Environmental Conservation Clemson UniversityClemsonSC29634USA

Global climate change can affect tree growth and carbon sink function by influencing plant carbohydrate synthesis and utilization,while elevation can be used as an ideal setting under natural conditions to simulate climate change effects.The effect of elevation on tree growth may depend on organ type.However,the allocation patterns of nonstructural and structural carbohydrates(NSCs and SCs,respectively)in different tree organs and their response to elevation remain unclear.We selected four dominant tree species,Schima superba,Castanopsis eyrei,Castanopsis fargesii and Michelia maudiae,along an elevation gradient from 609 to 1,207 m in subtropical evergreen broad-leaved forests and analyzed leaf,trunk,and fine root NSCs,carbon(C),nitrogen(N)and phosphorus(P)concentrations and the relative abundance of SCs.Leaf NSCs increased initially and then decreased,and trunk NSCs increased with increasing elevation.However,root NSCs decreased with increasing elevation.The relative abundance of SCs in leaves and trunks decreased,while the relative abundance of root SCs increased with increasing elevation.No significant correlations between SCs and NSCs in leaves were detected,while there were negative correlations between SCs and NSCs in trunks,roots,and all organs.Hierarchical partitioning analysis indicated that plant C/N and C/P were the main predictors of changes in SCs and NSCs.Our results suggest that tree organs have divergent responses to elevation and that increasing elevation will inhibit the aboveground part growth and enhance the root growth of trees.A tradeoff between the C distribution used for growth and storage was confirmed along the elevation gradient,which is mainly manifested in the"sink"organs of NSCs.Our results provide insight into tree growth in the context of global climate change scenarios in subtropical forest ecosystems.

关键词： Nonstructural carbohydrates Structural carbohydrates Elevation Subtropical evergreen broad-leaved forests Tree organs

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Assessment of soil total phosphorus storage in a complex topography along China's southeast coast based on multiple mapping scales

引用

Pedosphere 2024年第1期34卷 236-251页

作者： Zhongxing CHEN Jing LI Kai HUANG Miaomiao WEN Qianlai ZHUANG Licheng LIU Peng ZHU Zhenong JIN Shihe XING Liming ZHANG Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation College of Resources and EnvironmentFujian Agriculture and Forestry UniversityFuzhou 350002China State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China Guangxi Zhuang Autonomous Region plant protection station Nanjing 530022China Department of Earth Atmosphericand Planetary SciencesPurdue UniversityWest Lafayette IN47907US Department of Bioproducts and Biosystems Engineering University of Minnesota-Twin CitiesSaint Paul MN 55108USA Department of Geography The University of Hong KongHong Kong 999077China

Soil phosphorus (P) plays a vital role in both ecological and agricultural ecosystems, where total P (TP) in soil serves as a crucial indicator of soil fertility and quality. Most of the studies covered in the literature employ a single or narrow range of soil databases, which largely overlooks the impact of utilizing multiple mapping scales in estimating soil TP, especially in hilly topographies. In this study, Fujian Province, a subtropical hilly region along China’s southeast coast covered by a complex topographic environment, was taken as a case study. The influence of the mapping scale on soil TP storage (TPS)estimation was analyzed using six digital soil databases that were derived from 3 082 unique soil profiles at different mapping scales, i.e., 1:50 000 (S5),1:200 000 (S20), 1:500 000 (S50), 1:1 000 000 (S100), 1:4 000 000 (S400), and 1:10 000 000 (S1000). The regional TPS in the surface soil (0–20 cm) based on the S5, S20, S50, S100, S400, and S1000 soil maps was 20.72, 22.17, 23.06, 23.05, 22.04, and 23.48 Tg, respectively, and the corresponding TPS at0–100 cm soil depth was 80.98, 80.71, 85.00, 84.03, 82.96, and 86.72 Tg, respectively. By comparing soil TPS in the S20 to S1000 maps to that in the S5map, the relative deviations were 6.37%–13.32%for 0–20 cm and 0.33%–7.09%for 0–100 cm. Moreover, since the S20 map had the lowest relative deviation among different mapping scales as compared to S5, it could provide additional soil information and a richer soil environment than other smaller mapping scales. Our results also revealed that many uncertainties in soil TPS estimation originated from the lack of detailed soil information, i.e., representation and spatial variations among different soil types. From the time and labor perspectives, our work provides useful guidelines to identify the appropriate mapping scale for estimating regional soil TPS in areas like Fujian Province in subtropical China or other places with similar complex topographies. Moreover, it is of tremendous importance to accurately est

关键词： agricultural management appropriate mapping scale digitized conventional soil map estimation uncertainty subtropical hilly region

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

养分回收加剧了氮沉降背景下盐渍化草地优势植物内在氮磷的失衡

引用

Journal of plant Ecology 2024年第1期17卷 111-120页

作者： Yuan Su Shuaikai Wu Jie Hao Huajie Diao Kuanhu Dong Changhui Wang Shanxi Key Laboratory of Grassland Ecological protection and Native Grass Germplasm Innovation College of Grassland ScienceShanxi Agricultural UniversityTaigu 030801China Youyu Loess Plateau Grassland Ecosystem National Research station Shanxi Agricultural UniversityTaigu 030801China

在落叶之前,从衰老组织中重新吸收养分被认为是植物适应养分缺乏的一种策略。然而,养分重吸收如何调节植物体内氮(N)和磷(P)的平衡仍不清楚,特别是在土壤氮有效性增加的情况下。本文研究了不同速率(0、1、2、4、8、16、24和32 g N m^(-2... 详细信息

在落叶之前,从衰老组织中重新吸收养分被认为是植物适应养分缺乏的一种策略。然而,养分重吸收如何调节植物体内氮(N)和磷(P)的平衡仍不清楚,特别是在土壤氮有效性增加的情况下。本文研究了不同速率(0、1、2、4、8、16、24和32 g N m^(-2)yr^(-1))氮添加对中国北方盐渍化草地优势植物赖草(Leymus secalinus)叶片和茎秆养分回收的影响,以及养分回收在调控植物内部氮磷平衡的作用。研究结果表明:氮添加6年后,随着施氮量的增加,绿色和衰老组织(叶和茎)的N浓度和氮磷比均呈上升趋势。随着施氮量的增加,绿色组织中P浓度降低,而衰老组织中P浓度无显著变化。N重吸收效率(NRE)、P重吸收效率(PRE)和NRE:PRE比值沿N添加梯度显著降低。此外,我们发现衰老组织(叶和茎)氮磷比比绿色组织(叶和茎)氮磷比对氮素添加的响应更敏感,植物内在氮磷失衡的加剧主要是由于不成比例的减少养分重吸收,尤其是NRE。总的来说,我们的研究表明,NRE和PRE的差异进一步加剧了植物凋落物内部氮磷的失衡。

关键词：生态化学计量学氮添加氮磷失衡重吸收效率重吸收能力重吸收比盐渍化草地

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Possible source and migration pathway for early-summer immigrants of the oriental armyworm, Mythimna separata, arriving in northern Japan

引用

Journal of Integrative Agriculture 2023年第11期22卷 3474-3488页

作者： Akira OTUKA Tokumitsu NIIYAMA JIANG Xing-fu Institute for plant protection National Agriculture and Food Research OrganizationKoshi 861-1102Japan Akita plant protection station Akita 010-1232Japan Institute of plant protection Chinese Academy of Agricultural SciencesBeijing 100193P.R.China

The first generation of the oriental armyworm, Mythimna separata(Walker), arrives every year in northern Japan in mainly late May to early June. Analyses of weather maps suggested that this moth’s immigration source could be eastern China, but the accuracy of those analyses was very limited due to the lack of a current standard trajectory analysis. The management of migratory insect pests such as M. separata benefits from the identification of the migration source(s)and pathway(s) of the pests. The present study provides a trajectory analysis for M. separata. Backward trajectories from trap sites in northern Japan were calculated with the HYSPLIT System developed by the U.S. National Oceanic and Atmospheric Administration, taking the flight speed of M. separata and the limitation of low ambient temperature at flight height into account. The ending times of the moth’s short and long trajectories were set at dusk on the day before and two days before the possible arrival date, respectively. The results suggested two types of possible migration pathway: a multi-step pathway from Northeast China, the Korean Peninsula, and eastern Russia, which are destination areas of the first-generation’s migration, and a direct pathway from seasonal main emigration areas in eastern China such as Jiangsu and Shandong provinces. These findings contribute to our understanding of the migration ecology of M. separata and can be used for the development of methods to predict the migration of this insect.

关键词： migration backward trajectory oriental armyworm first generation East Asia

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：