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BnaWRKY75 positively regulates the resistance against Sclerotinia sclerotiorum in ornamental Brassica napus

Horticultural Plant Journal 2024年第3期10卷 784-796页

作者： Kexin Yu Yijie Zhang Xiaoyuan Fei Luyue Ma Rehman Sarwar Xiaoli Tan Zheng Wang School of Life Sciences Jiangsu UniversityZhenjiangJiangsu 212013China School of Food and Biological Engineering Jiangsu University ZhenjiangJiangsu 212013China Key Laboratory for Theory and Technology of intelligent agricultural Machinery and equipment Jiangsu UniversityZhenjiangJiangsu 212013China

With the development of tourism at home and abroad,Rapeseed(Brassica napus)has beco.e an important ornamental plant.However,its ornamental value at the inflorescence stage is greatly reduced by Sclerotinia sclerotiorum.Identification of important genes in the defense responses is critical for molecular breeding,which is an important strategy for co.trolling the disease.In this study,we isolated a B.napus WRKY transcription factor gene,BnaWRKY75.BnaWRKY75 was found to enco.e a nucleus-localized protein and exhibited relatively high expression in the stems.Arabidopsis thaliana transgenic plants expressing BnaWRKY75 showed enhanced resistance to S.sclerotiorum,and both ProBnaWRKY75:GUS and gene expression analyses showed that BnaWRKY75 was highly responsive to S.sclerotiorum infection,indicating the involvement of BnaWRKY75 in response to this infection.Furthermore,overexpression(OE)of BnaWRKY75 in B.napus significantly enhanced the resistance to S.sclerotiorum,whereas the resistance was reduced in RNAi transgenic B.napus plants.Moreover,the BnaWRKY75-OE B.napus plants exhibited co.stitutive activation of salicylic acid-,jasmonic acid-,and ethylene-mediated defense responses and the inhibition of both H_(2)O_(2)and O_(2)·^(-)accumulation in response to this pathogen.By co.trast,BnaWRKY75-RNAi plants showed a reverse pattern,suggesting that BnaWRKY75 is involved in hormonal signaling pathways and in the co.trol of reactive oxygen species accumulation.In co.clusion,these data indicate that BnaWRKY75,a regulator of multiple defense responses,positively regulates resistance against S.sclerotiorum,which may guide the improvement of resistance in rapeseed.

关键词： Brassica napus BnaWRKY75 Defense response Sclerotinia sclerotiorum

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Structure design and rice threshing performance of the variable-speed inertial pulley for simulating artificial threshing

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International Journal of agricultural and Biological Engineering 2024年第1期17卷 33-40页

作者： Tiantian Jing Zhong Tang Shuaihua Hao Cheng Shen Ting Wang Meilin Wang School of agricultural Engineering Jiangsu UniversityZhenjiang 212013JiangsuChina Key Laboratory of intelligent equipment and Robotics for Agriculture of Zhejiang Province Hangzhou 310058China co.lege of Engineering China Agricultural UniversityBeijing 100083China Nanjing Institute of agricultural Mechanization Ministry of Agriculture and Rural AffairsNanjing 210014China jiangsu World Agriculture Machinery co. Ltd(WORLD A/M)Zhenjiang 212311JiangsuChina

Due to the small models and co.pact structures,small harvesters have also caused problems such as poor threshing separation performance and large loss rates.In order to solve unstable cleaning effects of small harvesters when they facing different working co.ditions,this study selected rice plants in hilly areas for the experiment.Tensile breaking force of different parts of mature rice was tested,which revealed the fracture mechanism of each part under the co.bined force.Inertial threshing method was used to simulate artificial plate bin and design three kinds of non-circular pulley variable speed transmission threshing mechanism.With the help of transient inertia force,threshing force was co.pensated.This paper tested the harvesting performance of the variable speed threshing device with the help of the harvest performance test.Results show when facing the small rice planting area,the T/2 variable speed threshing device has better cleaning performance,and also the harvest loss rate of T/4 variable speed threshing device is the lowest.co.pared with the co.stant speed threshing device,the impurity co.tent rate of the variable speed threshing device is increased by 0.64%to 8.76%;the loss rate is reduced by 0.45%to 1.79%,which provides a basis for the optimization design of small co.bine harvester in hilly areas.

关键词： small harvesters fracture mechanism transient inertia force non-circular pulley

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Electronic structure and spin state regulation of vanadium nitride via a sulfur doping strategy toward flexible zinc-air batteries

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Journal of Energy Chemistry 2024年第2期89卷 239-249,I0007页

作者： Daijie Deng Honghui Zhang Jianchun Wu Xing Tang Min Ling Sihua Dong Li Xu Henan Li Huaming Li Institute for Energy Research Institute of Quantum and Sustainable TechnologySchool of Chemistry and Chemical EngineeringSchool of Materials Science and EngineeringJiangsu Province and Education Ministry Co-Sponsored Synergistic Innovation Center of Modern Agricultural EquipmentJiangsu UniversityZhenjiang 212013JiangsuChina Key Laboratory of Radio Frequency and Micro-Nano Electronics of jiangsu Province Nanjing 210023JiangsuChina Zhenjiang Kangyuan Electrical Appliance co. Ltd.Zhenjiang 212013JiangsuChina co.lege of Chemical and Biological Engineering Zhejiang UniversityHangzhou 310027ZhejiangChina YTO Group co.poration Dongfanghong(Henan)agricultural Service Technology co. Ltd.Luoyang 471033HenanChina

Owing to the distinctive structural characteristics,vanadium nitride(VN)is highly regarded as a catalyst for oxygen reduction reaction(ORR)in zinc-air batteries(ZABs).However,VN exhibits limited intrinsic ORR activity due to the weak adsorption ability to O-co.taining species.Here,the S-doped VN anchored on N,S-doped multi-dimensional carbon(S-VN/co.NS-MC)was co.structed using the solvothermal and in-situ doping methods.Inco.porating sulfur atoms into VN species alters the electron spin state of vanadium in the S-VN/co.NS-MC for regulating the adsorption energy of vanadium sites to oxygen molecules.The introduced sulfur atoms polarize the V 3d_(z)^(2) electrons,shifting spin-down electrons closer to the Fermi level in the S-VN/co.NS-MC.co.sequently,the introduction of sulfur atoms into VN species enhances the adsorption energy of vanadium sites for oxygen molecules.The*OOH dissociation transitions from being unspontaneous on the VN surface to a spontaneous state on the S-doped VN surface.Then,the ORR barrier on the S-VN/co.NS-MC surface is reduced.The S-VN/co.NS-MC demonstrates a higher half-wave potential and limiting current density co.pared to the VN/co.N-MC.The S-VN/co.NS-MC-based liquid ZABs display a power density of 195.7 m W cm^(-2),a specific capacity of 815.7 m A h g^(-1),and a cycling stability exceeding 250 h.The S-VN/co.NS-MC-based flexible ZABs are successfully employed to charge both a smart watch and a mobile phone.This approach holds promise for advancing the co.mercial utilization of VN-based catalysts in ZABs.

关键词： S-doped VN Electronic structures Spin state regulation Oxygen reduction reaction Zinc-air batteries

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Learning Label co.relations for Multi-Label Online Passive Aggressive Classification Algorithm

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Wuhan University Journal of Natural Sciences 2024年第1期29卷 51-58页

作者： ZHANG Yongwei Nanjing NARI intelligent Transport Technology co. Ltd.Nanjing 210061JiangsuChina

Label co.relations are an essential technique for data mining that solves the possible co.relation problem between different labels in multi-label classification.Although this technique is widely used in multi-label classification problems,batch learning deals with most issues,which co.sumes a lot of time and space resources.Unlike traditional batch learning methods,online learning represents a promising family of efficient and scalable machine learning algorithms for large-scale datasets.However,existing online learning research has done little to co.sider co.relations between labels.On the basis of existing research,this paper proposes a multi-label online learning algorithm based on label co.relations by maximizing the interval between related labels and unrelated labels in multi-label samples.We evaluate the performance of the proposed algorithm on several public datasets.Experiments show the effectiveness of our algorithm.

关键词： label co.relations passive aggressive multi-label classification online learning

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Polyoxometalates-derived nanostructures for electrocatalysis application

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Rare Metals 2024年第5期43卷 1845-1866页

作者： Chao-Yue Sun Wen Li Kai Wang Wei-Jia Zhou Hai-Qing Wang Institute for Advanced Interdisciplinary Research (iAIR) College of Chemistry and Chemical Engineering University of Jinan Shandong Electrical Engineering and equipment Group intelligent Electric co. Ltd.

The co.version of intermittent renewable electrical energy to chemical energy is of great importance which can not only mitigate current energy and environmental crisis but also co.tribute to the ongoing carbon neutrality national strategy.Electrocatalysis is serving as a low-carbon co.version technology that enables green and efficient energy co.version mainly through hydrogen evolution reaction （HER）,carbon dioxide reduction reaction（co.RR）,and nitrogen reduction reaction （NRR）.The co.e of electrocatalysis is the design and co.struction of low-co.t high-activity and high-stability electrocatalyst to drive reaction thermodynamics and kinetics.The employment of polyoxometalates （POMs） as platforms or precursors to co.struct different types of electrocatalysts has been widely reported.Herein,we systematically summarized the recent advances in POM-derived nanostructures for electrocatalysis application.The strategies for precursor design and electrocatalyst synthesis were briefly introduced.The morphology co.trol,phase co.trol,co.posite modulation,and heterostructure engineering in POM-derived nanostructures were presented in detail.The structure–activity relationship of POM-derived nanostructures is fully discussed for HER co.RR,and NRR applications.Finally,the current challenges and future outlooks of POM-derived nanostructures are summarized to provide insights toward high-efficiency electrocatalysts for energy co.version technologies.

关键词： Microforming Cylindrical co.pression Size effects Grain orientation Inhomogeneous deformation

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Surface roughness classification using light scattering matrix and deep learning

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Science China(Technological Sciences) 2024年第2期67卷 520-535页

作者： SUN Hao TAN Wei RUAN YiXiao BAI Long XU JianFeng State Key Laboratory of intelligent Manufacturing equipment and Technology School of Mechanical Science and EngineeringHuazhong University of Science and TechnologyWuhan 430074China Wuhan Digital Design and Manufacturing Innovation Center co. LtdWuhan 430074China

High-quality optical co.ponents have been widely used in various applications;thus,extremely high beam quality is required.Moreover,surface roughness is a key indicator of the surface quality.In this study,the angular distribution of light scattering field intensity was obtained for surfaces having different roughness profiles based on the finite difference time domain(FDTD)method,and the results were co.pared with those obtained using the generalized Harvey-Shack(GHS)theory.It was shown that the FDTD approach can be used for an accurate simulation of the scattered field of a rough surface,and the superposition of results obtained from many surfaces that have the same roughness level was in good agreement with the result given by the analytic GHS model.A light scattering matrix(LSM)method was proposed based on the FDTD simulation results that co.ld obtain rich surface roughness information.The classification effect of LSM was co.pared with that of the single-incidence scattering distribution(SISD)based on a ResNet-50 deep learning network.The classification accuracy of the model trained with the LSM dataset was obtained as 95.74%,which was 23.40%higher than that trained using the SISD dataset.Moreover,the effects of different noise types and filtering methods on the classification performance were analyzed,and the LSM was also shown to improve the robustness and generalizability of the trained surface roughness classifier.Overall,the proposed LSM method has important implications for improving the data acquisition scheme of current light scattering measurement systems,and it also has the potential to be used for detection and characterization of surface defects of optical co.ponents.

关键词： surface roughness FDTD simulation GHS theory deep learning light scattering matrix

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Experimental Investigation on Solution Regeneration Performance and co.fficients in Full-Open System for Heat and Water Reco.ery of Flue Gas

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Journal of Thermal Science 2024年第3期33卷 1094-1108页

作者： WEI Hongyang HUANG Shifang MA Yuxin CHEN Bo SUN Li ZHANG Xiaosong School of Energy and Environment Southeast UniversityNanjing210096China MOE Engineering Research Center for Building Energy equipment&Environment Southeast UniversityNanjing210096China jiangsu Frontier Electric Technology co. LtdNanjing211106China

The reco.ery of heat and water from low-grade flue gas is of co.siderable importance for energy co.servation and environmental preservation.While the full-open absorption heat pump shows promise as a means of achieving heat and water reco.ery,the lack of research on heat and mass transfer performance of open-type solution evaporation regeneration represents a significant impediment to its design and operation.This paper experimentally investigates the regeneration performance of an open-type spaying tower equipped with ceramic structured packings.Two different regeneration modes are proposed,namely ambient air receiver mode and flue gas receiver mode,to utilize air or low-grade flue gas as a driving source.The impact of different input parameters on the regeneration characteristics,including heat transfer capacity,water removal rate,thermal efficiency,and humidity effectiveness,are demonstrated.The findings indicate that the enhancement of regeneration can be achieved through the increase of solution flow rate,solution temperature,and flue gas flow rate in both regeneration modes.However,high solution co.centration and flue gas humidity ratio can weaken water removal rates and reduce thermal efficiency.For the regeneration of CaCl_(2)-H_(2)O with a co.centration of55%,flue gas around 200℃with a humidity ratio below 44 g/kg can successfully drive the solution regeneration process.When the solution co.centration or flue gas humidity ratio co.tinues to rise,additional energy is necessary for regeneration.Furthermore,the co.pled heat and mass transfer co.fficients are fitted,which can co.tribute to the design and optimization of the open-type regenerator.

关键词： heat and water reco.ery solution regeneration flue gas regeneration performance co.fficient

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Mechanism of a dual-phase Ti-Nb alloy exhibiting near-linear elastic deformation

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Rare Metals 2024年第5期43卷 2282-2289页

作者： Yan Guo Wang Ding Wen Ma Shun Guo Hao-Dong Meng Nian-Yao Xu School of Automotive Engineering Changzhou Institute of Technology School of Materials Science and Engineering Jiangsu University Youke Publishing co. Ltd China Grinm Group Co. Ltd

In this work, the underlying mechanism responsible for the near-linear elastic deformation behavior of a dual-phase Ti-Nb alloy co.sisting of β and a''phase with large reco.erable strain was systematically elucidated.Based on in situ synchrotron X-ray diffraction(SXRD)analyses, it was found that besides intrinsic elastic deformation, a slight reversible β ? a''stress-induced martensitic(SIM) transformation, which proceeded in a co.secutive mode under the retarding effect of micro-defects, took place during the near-linear elastic deformation.After unloading, a small amount of residual macrosco.ic strain remained in the specimen due to the inco.plete reverse a''→ β transformation on unloading. The high near-linear elastic deformability of the co.d drawing(CD)Ti-Nb alloy has been revealed to be attributed to the co.pling actions of intrinsic elasticity as well as the co.secutive and reversible β ? a''SIM transformation. Our research may co.tribute to a new avenue for the design and development of novel dual-phase Ti-based alloys with desirable elastic deformability.

关键词： Titanium alloys Dual-phase Near-linear elastic deformation Martensitic transformation

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A co.parative investigation on wear behaviors of physical and chemical vapor deposited bronze co.tings for hydraulic piston pump

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Chinese Journal of Aeronautics 2024年第3期37卷 391-403页

作者： Wei HAO Andrews LARBI Hongkang DONG Dongyun WANG Shenghu LI Zhejiang Provincial Key Laboratory of Urban Rail Transit intelligent Operation and Maintenance Technology and equipment Zhejiang Normal UniversityJinhua 321004China co.lege of Engineering Zhejiang Normal UniversityJinhua 321004China Hangzhou Lilong Hydraulic co. LtdHangzhou 311200China

The cylinder block/valve plate interface is one of the most critical frictional interfaces of the swashplate-type axial piston pump.However,the poor lubrication interface caused rapid wear and high friction loss in an elastohydrodynamic lubrication system,decreasing the pump lifetime.Wear resistant bronze co.tings were fabricated on 38CrMoAl substrate by Physical Vapor Deposition(PVD)and Chemical Vapor Deposition(CVD),respectively.Ball-on-disc wear tests were performed to co.paratively investigate the wear behaviors of the co.tings and bulk ductile iron samples.It can be found that the PVD-bronze co.ting exhibited better wear resistance than the other two samples.This enhanced wear resistance was attributed to the unique co.posite microstructure and desired mechanical strength,which co.ld resist to mechanical shear and spallation,decreasing friction loss.The appropriate hardness of(1.33±0.07)GPa co.ld be beneficial for enhancing its wear resistance.The PVD-bronze co.ting possessed a much lower and stable co.fficient of friction(about 0.1)and wear rate(about 6000μm3.N-1.m-1)under the loading forces of about 100 N after 20 min.The wear mechanism was the abrasive wear.

关键词： Aircraft hydraulic pump Bronze co.tings Wear resistance Mechanisms

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A novel integrated microfluidic chip for on-demand electrostatic droplet charging and sorting

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Bio-Design and Manufacturing 2024年第1期7卷 31-42页

作者： Jinhui Yao Chunhua He Jianxin Wang Canfeng Yang Ye Jiang Zhiyong Liu Guanglan Liao Tielin Shi State Key Laboratory of intelligent Manufacturing equipment and Technology Huazhong University of Science and TechnologyWuhan 430074China

On-demand droplet sorting is extensively applied for the efficient manipulation and genome-wide analysis of individual cells.However,state-of-the-art microfluidic chips for droplet sorting still suffer from low sorting speeds,sample loss,and labor-intensive preparation procedures.Here,we demonstrate the development of a novel microfluidic chip that integrates droplet generation,on-demand electrostatic droplet charging,and high-throughput sorting.The charging electrode is a co.per wire buried above the nozzle of the microchannel,and the deflecting electrode is the phosphate buffered saline in the microchannel,which greatly simplifies the structure and fabrication process of the chip.Moreover,this chip is capable of high-frequency droplet generation and sorting,with a frequency of 11.757 kHz in the drop state.The chip co.pletes the selective charging process via electrostatic induction during droplet generation.On-demand charged microdroplets can arbitrarilymove to specific exit channels in a three-dimensional(3D)-deflected electric field,which can be co.trolled acco.ding to user requirements,and the flux of droplet deflection is thereby significantly enhanced.Furthermore,a lossless modification strategy is presented to improve the accuracy of droplet deflection or harvest rate from 97.49% to 99.38% by monitoring the frequency of droplet generation in real time and feeding it back to the charging signal.This chip has great potential for quantitative processing and analysis of single cells for elucidating cell-to-cell variations.

关键词： co.per wire Droplet generation Droplet sorting Microfluidic chips On-demand charging

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