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正丁基硫代磷酰三胺对体细胞和生殖细胞的致突变性研究

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癌变．畸变．突变 2016年第6期28卷 432-437页

作者：原野陈丹丹荆淑芳于永生陈健恒吴纯启施畅军事医学科学院毒物药物研究所国家北京药物安全评价研究中心北京100850 军事医学科学院抗毒药物与毒理学国家重点实验室北京100850 中国食品药品检定研究院北京100050 YMS agriculture International co.p. Toronto M5X 1C7OntarioCanada

目的:采用小鼠骨髓细胞染色体畸变试验和小鼠显性致死突变试验评价正丁基硫代磷酰三胺(NBPT)对体细胞和生殖细胞的致突变性。方法:小鼠骨髓细胞染色体畸变试验中雄性小鼠分别单次灌胃给予NBPT 250、500、1 000 mg/kg,1 000 mg/kg组动物... 详细信息

目的:采用小鼠骨髓细胞染色体畸变试验和小鼠显性致死突变试验评价正丁基硫代磷酰三胺(NBPT)对体细胞和生殖细胞的致突变性。方法:小鼠骨髓细胞染色体畸变试验中雄性小鼠分别单次灌胃给予NBPT 250、500、1 000 mg/kg,1 000 mg/kg组动物于给药后24、48及72 h取骨髓细胞制备骨髓片,其他组动物于给药后24 h取骨髓细胞制备骨髓片。每只动物在油镜下分析100个中期相骨髓细胞,记录染色体结构畸变、数目畸变和发生裂隙的细胞数。小鼠显性致死试验中雄鼠分别连续灌胃给予NBPT 250、500、1 000 mg/kg 5 d后,雌鼠与雄鼠同笼交配5 d,雄鼠再于2 d后与下一轮的雌鼠交配,共进行7个轮次的交配,每轮次1周,覆盖雄性小鼠整个生精周期。每轮次雌鼠于同笼结束后第14天处死,计数总着床数、黄体数、死胎、活胎和吸收胎数。结果:小鼠单次灌胃NBPT 24 h后,250、500、1 000 mg/kg剂量组骨髓细胞染色体结构畸变率分别为2.4%、3.0%和2.0%,1 000 mg/kg组24、48和72 h的骨髓细胞染色体结构畸变率均未超过4%,与溶媒对照组比较差异均无统计学意义(P>0.05)。各NBPT处理组染色体四倍体与裂隙的发生率也未出现与NBPT相关性的变化。小鼠显性致死试验的7个交配轮次中,NBPT 3个处理组受孕率为100%,母鼠平均着床数、黄体数、活胎数与溶媒对照组比较差异均无统计学意义(P>0.05)。仅在NBPT 250 mg/kg组发现第4交配轮次母鼠着床前丢失率增高,第6轮次母鼠窝均非活胎数降低,与溶媒对照组比较差异均有统计学意义(P<0.01)。NBPT 3个处理组各交配轮次母鼠的致死突变率大多为负值,偶见较低的正值。结论:在本试验条件下,NBPT无致小鼠骨髓细胞染色体畸变效应,对雄性ICR小鼠无显性致死突变作用。

关键词：正丁基硫代磷酰三胺致突变性骨髓细胞染色体畸变试验显性致死试验

Safety and immunogenicity of inactivated SARS-co.-2 vaccine in high-risk occupational population:a randomized,parallel,co.trolled clinical trial

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Infectious Diseases of Poverty 2021年第6期10卷 27-37页

作者： Yongliang Feng Jing Chen Tian Yao Yue Chang Xiaoqing Li Rongqin Xing Hong Li Ruixue Xie Xiaohong Zhang Zhiyun Wei Shengcai Mu Ling Liu Lizhong Feng Suping Wang Department of Epidemiology School of Public HealthShanxi Medical University56 Xinjian South RoadTaiyuan 030001Shanxi ProvinceChina Center of Clinical Epidemiology and Evidence Based Medicine Shanxi Medical UniversityTaiyuanChina Shanxi Provincial Center for Disease co.trol and Prevention 8 Xiaonanguan StreetTaiyuan 030012Shanxi ProvinceChina Shanxi Provincial Key Laboratory for Major Infectious Disease Response TaiyuanChina Outpatient Department of Shanxi Aviation Industry Group co.LTD TaiyuanChina

Background: Severe acute respiratory syndrome co.onavirus 2 (SARS-co.-2) infection and the resulting co.onavirus disease 2019 (co.ID-19) have a substantial burden on health-care systems around the world. This is a randomized parallel co.trolled trial for assessment of the immunogenicity and safety of an inactivated SARS-co.-2 vaccine, aiming to determine an appropriate vaccination interval of the vaccine for high-risk occupational ***: In an ongoing randomized, parallel, co.trolled phase IV trial between January and May 2021 in Taiyuan City, Shanxi Province, China, we randomly assigned the airport ground staff and public security officers aged 18 to 59 years to receive two doses of inactivated SARS-co.-2 vaccine at 14 days, 21 days, or 28 days. The serum neutralizing antibody to live SARS-co.-2 was performed at baseline and 28 days after immunization. Long-term data are being co.lected. The primary immunogenicity endpoints were neutralization antibody seroco.version and geometric mean titer (GMT) at 28 days after the seco.d dose. Analysis of variance (ANOVA), chi-square, and logistic regression analysis were used for data ***: A total of 809 participants underwent randomization and received two doses of injections: 270, 270, 269 in the 0-14, 0-21, and 0-28 vaccination group, respectively. By day 28 after the seco.d injection, SARS-co.-2 neutralizing antibody of GMT was 98.4 (95%CI: 88.4-108.4) in the 0-14 group, which was significantly lower co.pared with 134.4 (95%CI: 123.1-145.7) in the 0-21 group (P co.version rates to neutralizing antibodies (GMT ≥ 16) of 100.0% for all three groups, respectively. The intention-to-treat (ITT) analysis yielded similar results. All reported adverse reactions were ***: Both a two-dose of inactivated SARS-co.-2 vaccine at 0-21 days and 0-28 days regimens significantly improv

关键词： co.ID-19 Inactivated SARS-co.-2 vaccine Immunogenicity Safety High-risk occupational population Randomized co.trolled trial

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不同母质发育橡胶林土壤微生物特征研究

西南农业学报 2022年第4期35卷 964-971页

作者：赵春梅王文斌 Didier Lesueur 张永发薛欣欣罗雪华李晓波中国热带农业科学院土壤肥料研究中心农业农村部橡胶树生物学与遗传资源利用重点实验室省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室中国热带农业科学院橡胶研究所海口571101 CIAT Asia-International Center for Tropical agriculture(CIAT) Agricultural Genetics InstitutePham Van DongTu LiemHanoiVietnam-opposite the Ministry of Public Security 海南农垦南繁产业集团有限公司三亚572022

【目的】研究海南不同母质发育橡胶林土壤理化性状和土壤微生物量分布特征,分析不同母质之间肥力差异,探究影响橡胶林土壤肥力的环境影响因素,旨在为海南不同土壤条件下橡胶林施肥管理和土壤质量提升提供参考。【方法】以海南四大成土母... 详细信息

【目的】研究海南不同母质发育橡胶林土壤理化性状和土壤微生物量分布特征,分析不同母质之间肥力差异,探究影响橡胶林土壤肥力的环境影响因素,旨在为海南不同土壤条件下橡胶林施肥管理和土壤质量提升提供参考。【方法】以海南四大成土母质(花岗岩、玄武岩、变质岩和浅海沉积物)土壤为研究对象,通过调查取样和相关性分析、冗余分析等统计方法,对不同母质条件下橡胶林土壤含水量、有机碳等13项理化指标和微生物碳等微生物指标及两者之间的相关性进行了研究。【结果】不同母质发育橡胶林土壤在物理性状、化学成分、微生物量等方面均存在显著差异,整体上玄武岩理化指标和微生物量含量最高,其理化性状与微生物特征优于其他3种母质。主成分分析表明,不同母质土壤肥力综合得分分别为1.90(玄武岩)、1.88(浅海沉积物)、1.59(变质岩)、-5.37(花岗岩),影响土壤肥力的主要因素是微生物量氮(MBN)、微生物量碳(MBC)、含水量、土壤温度、有效氮等指标。冗余分析表明,土壤微生物与土壤理化性状之间具有极显著差异,影响微生物量含量的主要理化因子为土壤含水量、pH、全钾、土壤温度、全磷、有效氮等。【结论】说明橡胶林土壤微生物和土壤理化性质之间关系密切,并且不同母质土壤肥力的影响因素有所不同,因此在橡胶林土壤质量评价与管理等研究过程中,应充分考虑不同母质与其他耕作条件下土壤微生物和理化性状的变化特征及其耦合关系。

关键词：母质理化性状微生物生物量相关性

Republish:Open letter on the crucial role of fungi in preserving and enhancing biodiversity

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Studies in Fungi 2022年第1期7卷 183-183页

作者： Marc Palahí Toby Kiers Merlin Sheldrake Giuliana Furci Robert Nasi Rodríguez-Garavito César Director European Forest Institute Director Society for the Protection of Underground Networks Author of Entangled Life Executive director Fungi Foundation&co-chair IUCN SSC Fungal Conservation Committee Chief Executive Officer CIFOR-ICRAF Professor of Clinical Law and Director Earth Rights Advocacy ClinicNew York University School of Law

When we think of forests we usually think of trees,plants and *** forests co.ld not exist without fungi,which lie at the base of the biodiversity webs that support much of life on *** fungi live as branching,fusing networks of tubular cells known as mycelium which can make up between a third and a half of the living mass of ***,the total length of fungal mycelium in the top 10cm of soil is more than 450 quadrillion km:about half the width of our *** networks co.prise an ancient life-support system that easily qualifies as one of the wonders of the living *** that,fungi represent a meagre 0.2%of our global co.servation priorities.

关键词： soil biodiversity letter

A cluster-randomized trial co.paring two SWITCH implementation support strategies for school wellness intervention effectiveness

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Journal of Sport and Health Science 2023年第1期12卷 87-96页

作者： Richard R.Rosenkranz Philip M.Dixon David A.Dzewaltowski Gabriella M.McLoughlin Joey A.Lee Senlin Chen Spyridoula Vazou Lorraine M.Lanningham-Foster Doug A.Gentile Gregory J.Welk Department of Food NutritionDietetics&HealthKansas State UniversityManhattanKS 66506USA Department of Statistics Snedecor HallIowa State UniversityAmesIA 50011-1210USA Department of Health Promotion University of NebraskaOmahaNE 68198-4365USA Implementation Science Center for Cancer co.trol and Prevention Research Center Brown SchoolWashington University in St.LouisSt.LouisMO 63130USA Division of Public Health Sciences(Department of Surgery) Washington University School of MedicineWashington University in St.LouisSt.LouisMO 63130USA Department of Health Sciences University of Colorado Colorado SpringsColorado SpringsCO 80918USA Department of Kinesiology Iowa State UniversityAmesIA 50011-4008USA Department of Food Science&Human Nutrition Iowa State UniversityAmesIA 50011USA Department of Psychology Iowa State UniversityAmesIA 50011-1041USA

Background:The School Wellness Integration Targeting Child Health(SWITCH)intervention has demonstrated feasibility as an implementation approach to help schools facilitate changes in students’physical activity(PA),sedentary screen time(SST),and dietary intake(DI).This study evaluated the co.parative effectiveness of enhanced(individualized)implementation and standard(group-based)***:Twenty-two Iowa elementary schools participated,with each receiving standardized training(wellness co.ference and webinars).Schools were matched within region and randomized to receive either individualized or group implementation *** PA,SST,and DI outco.es of 1097 students were assessed at pre-and post-intervention periods using the Youth Activity *** mixed models evaluated differential change in outco.es by co.dition,for co.parative effectiveness,and by ***:Both implementation co.ditions led to significant improvements in PA and SST over time(pco.mensurately(p value range:0.02‒0.05).There were no differential changes between the group and individualized co.ditions for PA(p=0.51),SST(p=0.19),or DI(p=0.73).There were no differential effects by gender(i.e.,non-significant co.dition-by-gender interactions)for PA(p_(for interaction)=0.86),SST(p_(for interaction)=0.46),or DI(p_(for interaction)=0.15).Effect sizes for both co.ditions equated to approximately 6 min more PA per day and approximately 3 min less sedentary ***:The observed lack of difference in outco.es suggests that group implementation of SWITCH is equally effective as individualized implementation for building capacity in school wellness ***,the lack of interaction by gender suggests that SWITCH can be beneficial for both boys and *** research is needed to understand the school-level factors that influence implementation(and outco.es)of SWITCH.

关键词： Children Implementation science Obesity prevention Physical activity School health

Trivalent SARS-co.-2 virus-like particle vaccines exhibit broad-spectrum neutralization and protection against XBB.1 and BA.2.86 variants

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Virologica Sinica 2024年第5期39卷 836-839页

作者： Lu Zhang Siyu Tian Jun Dai Yuanyuan Li Yu Zhou Yan Li Jiao Xu Shuyun Liu Zhiwei Lin Zhaoyong Zhang Jiantao Chen Peilan Wei Jingxian Zhao Jing Jin Yanqun Wang Jincun Zhao State Key Laboratory of Respiratory Disease Public Health Safety Center Laboratory of General Administration of CustomsGuangzhou Customs Technology CenterGuangzhouGuangdong510182China Patronus Biotech co.Ltd. Guangzhou510182China State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory Healththe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdong510182China Guangzhou National Laboratory GuangzhouGuangdong510182China Clinical Laboratory Medicine Department The Second Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdong510182China Shanghai Institute for Advanced Immunochemical Studies School of Life Science and TechnologyShanghaiTech UniversityShanghai201210China Institute for Hepatology National Clinical Research Center for Infectious DiseaseShenzhen Third People’s HospitalThe Second Affiliated HospitalSchool of MedicineSouthern University of Science and TechnologyShenzhen518000China

Dear Editor,The newly emerged severe acute respiratory syndrome co.onavirus 2(SARS-co.-2)Omicron subvariants(XBB.1,EG.5 and JN.1)and sublineages have been circulating globally with superior growth advantages over other Omicron variants(Tamura et al.,2023).However,seco.d-generation vaccines,including the BA.2 or BA.5 bivalent vaccine boosters,did not produce robust neutralization against the newly emerged XBB.1 or EG.5(Zou et al.,2023).co.pared with variants BA.2 and BA.5,XBB.1 carries more mutations in the receptor binding domain(RBD)and exhibits significant immune evasion(Wang et al.,2023b).XBB.1 and its descendent lineages have rapidly beco.e the dominant SARS-co.-2 strain and are causing the next global wave of ***,it is essential to develop an updated vaccine against the newly emerged SARS-co.-2 variants.

关键词： vaccine vaccines acute

co.served Cytochrome P450 Evolved in Seed Plants Regulates Flower Maturation

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Molecular Plant 2015年第12期8卷 1751-1765页

作者： Zhenhua Liu Benoit Boachon RaphaelLugan Raquel Tavares Mathieu Erhardt Jerome Mutterer Valerie Demais Stephanie Pateyron Veronique Brunaud Toshiyuki Ohnishi Ales Pencik Patrick Achard Fan Gong Peter Hedden Daniele Werck-Reichhart Hugues Renault Institute of Plant Molecular Biology Centre National de la Recherche Scientifique (CNRS) University of Strasbourg 67084 Strasbourg France Laboratoire de Biometrie et Biologie Evolutive Universite Lyon 1 CNRS 69622 Villeurbanne France plateforme d'lmagerie In Vitro IFR 37 de Neurosciences 67084 Strasbourg France Transcriptomic Platform Unite de Recherche en Genomique Vegetale (URGV) INRA Universite d'Evry Val d'Essonne CNRS 91057 Evry France Bioinformatics for Predictive Genomics URGV INRA Universite d'Evry Val d'Essonne CNRS 91057 Evry France Graduate School of agriculture Shizuoka University Shizuoka 422-8529 Japan Laboratory of Growth Regulators & Department of Chemical Biology and Genetics Centre of the Region Hana for Biotechnological and Agricultural Research Faculty of Science Palacky University & Institute of Experimental Botany AS CR 771 47 Olomouc Czech Republic Rothamsted Research Harpenden Hertfordshire AL5 2JQ UK University of Strasbourg Institute for Advanced Study (USIAS) 67084 Strasbourg France Freiburg institute for Advanced Studies (FRIAS) University of Freiburg 79104 Freiburg Germany Present address: 158 Emerson Hall Section of Plant Biology School of Integrative Plant Science Cornell University Ithaca NY 14853 USA present address: Laboratoire Physiologie des Fruits et Legumes - EA 4279 Campus Agroparc Avignon France present address: Home Office Science - Centre for Applied Science and Technology Woodcock Hill Sandridge St Albans HeFts AL4 9HQ UK

Global inspection of plant genomes identifies genes maintained in low co.ies across taxa and under strong purifying selection, which are likely to have essential functions. Based on this rationale, we investigated the function of the low-duplicated CYP715 cytochrome P450 gene family that appeared early in seed plants and evolved under strong negative selection. Arabidopsis CYP715A 1 showed a restricted tissue-specific expres- sion in the tapetum of flower buds and in the anther filaments upon anthesis, cyp715a1 insertion lines showed a strong defect in petal development, and transient alteration of pollen intine deposition. co.parative expres- sion analysis revealed the downregulated expression of genes involved in pollen development, cell wall biogenesis, hormone homeostasis, and floral sesquiterpene biosynthesis, especially TPS21 and several key genes regulating floral development such as MYB21, MYB24, and MYC2. Acco.dingly, floral sesquiterpene emission was suppressed in the cyp715a1 mutants. Flower hormone profiling, in addition, indicated a modi- fication of gibberellin homeostasis and a strong disturbance of the turnover of jasmonic acid derivatives. Petal growth was partially restored by the active gibberellin GA3 or the functional analog of jasmonoyl-isoleucine, co.onatine. CYP715 appears to function as a key regulator of flower maturation, synchronizing petal expan- sion and volatile emission. It is thus expected to be an important determinant of flower-insect interaction.

关键词： flower development phylogenomics negative selection jasmonate gibberellins volatile co.pounds

Floristic diversity and carbon stocks in the periphery of Deng–Deng National Park,Eastern Cameroon

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Journal of Forestry Research 2020年第3期31卷 989-1003页

作者： Louis Paul Roger Kabelong Banoho Louis Zapfack Robert Bertrand Weladji Cedric Chimi Djomo Melanie Chichi Nyako Yannick Enock Bocko Damien Marie Essono Julliete Mancho Nasang Nade`ge Madountsap Tagnang Charles Innocent Memvi Abessolo Kenneth Roger Mvondo Sakouma Farikou Mamadou Souahibou Florence Jeanne Sarah Palla Tonga Ketchatang Peguy Remi Jiagho Thierry Loic Kenmou Ulrich Arnaud Choumele Kana Jumo Boris Armel Anyam Yi Andjik Roger Bruno Tabue Mbobda Department of Plant Biology Faculty of ScienceUniversity of Yaounde IBP 812 YaoundeCameroon IUCN World co.mission On Protected Areas Co-Vice Chair Commission Mondiale des Aires Protegees-Afrique Centrale(CMAP-UICN)GlandSwitzerland Organisation pour la co.servation et le Developpement(OCD) BP 33323 YaoundeCameroun Department of Biology Concordia University7141 Sherbrooke St.W.MontrealMontrealQC H4B 1R6Canada Institute of Agricultural Research for Development(IRAD) PO.BOX 2123YaoundeCameroon Laboratoire de Botanique et Eco.ogie Facultedes Sciences et TechniquesUniversiteMarien NgouabiBrazzavilleCongo Ministry of Forestry and Wildlife BP 34430 YaoundeCameroon Programme National de Developpement Participatif(PNDP) YaoundeCameroun Observatoire des Forets du Bassin du co.go(OFAC) BP 20818 YaoundeCameroun Fokabs International PO BOX 14097YaoundeCameroon International Union for co.servation of Nature Programme CamerounBP:5506 YaoundeCameroun Secretariat Technique REDD BP 320 YaoundeCameroun

Carbon is co.tinuously being removed from the atmosphere by photosynthesis and stored in carbon pools(live,dead,and soil carbon)of forest ***,carbon stock in dead wood and of trees with diameters at breast height(dbh)between 5 and 10 cm is often not co.sidered in many studies carried out in the co.go Basin *** relationship between tree diversity,life-forms and carbon stocks has received little *** study was carried out on the outskirts of Deng Deng National Park(DDNP)to determine tree diversity(dominant families,species richness and Shannon index),assess carbon stocks in the five carbon co.partments(living tree,understory,fine roots,dead wood and litter)as well as to analyze the relationship between(1)carbon stocks and tree diversity;and,(2)between carbon stock and *** Shannon index of trees≥10 cm dbh ranged from 2.6 in riparian forest to 4.3 in seco.dary forest;and for the tree between 5 and 10 cm,it ranged to 1.56 in riparian forest to 3.68 in the seco.dary *** study site housed 16 species,7 genera and 3 families which are only found in trees of dbh between 5 and 10 *** average total carbon stock of the five co.partments varied from 200.1 t ha-1 in forest residues to 439.1 t ha-1 in seco.dary *** wood carbon stock varied from 1.2 t ha-1 in riparian forests to 12.51t ha-1 in *** above ground carbon stocks for trees with diameter between 5 and 10 cm varied from 0.7 t ha-1 in young fallow fields to 5.02 t ha-1 in old seco.dary *** study reveals a low but positive co.relation between species richness and total carbon stocks,as well as a significant positive relationship between life-forms and total carbon *** findings highlight the need for more data co.cerning carbon co.tent of dead wood,carbon of trees≥5 cmstocks and tree diversity from other areas of the co.go Basin for a good understanding of the co.tribution of tropical forests

关键词： Deng Deng national park Carbon stocks Land co.er types Species diversity Dead wood

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HPLC测定经肉苁蓉药材中松果菊苷的含量

南方农业 2018年第29期12卷 124-127页

作者：魏文强王翠艳内蒙古工业大学化工学院内蒙古呼和浩特010051 甘肃省通渭县食品药品监督管理局甘肃通渭743300 内蒙古co.捕集与资源化工程中心内蒙古呼和浩特010051 内蒙古煤基co.捕集与资源化团队内蒙古呼和浩特010051

目的:HPLC测定在液氮条件研磨以及在自然风干条件下研磨不同种肉苁蓉中松果菊苷的含量,比较野生肉苁蓉和人工培养的肉苁蓉中松果菊苷含量。方法:采用日本岛津LC-20A高效液相色谱仪测定。色谱柱为YMC-Inertsil ODS-SP(4.6 mm×150 mm,5μ... 详细信息

目的:HPLC测定在液氮条件研磨以及在自然风干条件下研磨不同种肉苁蓉中松果菊苷的含量,比较野生肉苁蓉和人工培养的肉苁蓉中松果菊苷含量。方法:采用日本岛津LC-20A高效液相色谱仪测定。色谱柱为YMC-Inertsil ODS-SP(4.6 mm×150 mm,5μm;YMC co.Ltd,Japan);流动相:乙腈-甲醇-1%甲酸(10∶15∶75);检测波长334 nm;流速0.7 mg·m L-1。柱温30℃。结果:处理条件的不同对松果菊苷含量有非常明显影响,液氮条件下研磨的肉苁蓉中松果菊苷的含量普遍高于自然风干条件下研磨的肉苁蓉;野生肉苁蓉的有效成分含量要远高于人工培养。

关键词：肉苁蓉松果菊苷高效液相色谱法