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A Comprehensive Approach for the Clustering of Similar-Performance Cells for the Design of a Lithium-Ion Battery Module for Electric Vehicles

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Engineering 2019年第4期5卷 795-802页

作者： Wei Li Siqi Chen Xiongbin Peng Mi Xiao Liang Gao Akhil Garg Nengsheng Bao State Key Lab of Digital Manufacturing Equipment and Technology Huazhong University of Science and TechnologyWuhan 430074China Key Lab of Mechatronic systems Intelligent integration Technology Ministry of EducationShantou UniversityShantou 515063China

An energy-storage system comprised of lithium-ion battery modules is considered to be a core component of new energy vehicles,as it provides the main power source for the transmission ***,manufacturing defects in battery modules lead to variations in performance among the cells used in series or parallel *** variation results in incomplete charge and discharge of batteries and non-uniform temperature distribution,which further lead to reduction of cycle life and battery capacity over *** solve this problem,this work uses experimental and numerical methods to conduct a comprehensive investigation on the clustering of battery cells with similar performance in order to produce a battery module with improved electrochemical *** were first performed by dismantling battery modules for the measurement of performance *** kmeans clustering and support vector clustering(SVC)algorithms were then employed to produce battery modules composed of 12 cells *** verification of the results obtained from the clustering analysis was performed by measuring the temperature rise in the cells over a certain period,while air cooling was *** was found that the SVC-clustered battery module in Category 3 exhibited the best performance,with a maximum observed temperature of 32℃.By contrast,the maximum observed temperatures of the other battery modules were higher,at 40℃for Category 1(manufacturer),36℃for Category 2(manufacturer),and 35℃for Category 4(k-means-clustered battery module).

关键词： Clustering algorithm Battery module Equalization Electric vehicle

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Kalman Filtering for Delayed Singular systems with Multiplicative Noise

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IEEE/CAA Journal of Automatica Sinica 2016年第1期3卷 51-58页

作者： Xiao Lu Linglong Wang Haixia Wang Xianghua Wang Key Laboratory for robot and Intelligent Technology of Shandong province Shandong University of Science and Technology College of Transportation Shandong University of Science and Technology

Kalman filtering problem for singular systems is dealt with, where the measurements consist of instantaneous measurements and delayed ones, and the plant includes multiplicative noise. By utilizing standard singular value decomposition, the restricted equivalent delayed system is presented, and the Kalman filters for the restricted equivalent system are given by using the well-known re-organization of innovation analysis lemma. The optimal Kalman filter for the original system is given based on the above Kalman filter by recursive Riccati equations, and a numerical example is presented to show the validity and efficiency of the proposed approach, where the comparison between the filter and predictor is also given. © 2014 Chinese Association of Automation.

关键词： Estimation Filtration Phase noise Riccati equations Singular value decomposition

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Multi-functional stretchable and flexible sensor array to determine the location, shape, and pressure: Application in a smart robot

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Science China(Technological Sciences) 2018年第8期61卷 1137-1143页

作者： HAN LiBiao DING JianNing WANG Shuai XU Jiang YUAN NingYi CHENG GuangGui LIU ZunFeng jiangsu Collaborative innovation Center of Photovoltaic Science and Engineering Changzhou University Micro/Nano Science and Technology Center Jiangsu University State Key Laboratory of Medicinal Chemical Biology Key Laboratory of Functional Polymer

The rapid increase in the aging population prompts the development of wearable devices and sophisticated robots. With their ability to collect complex information about their surroundings via e-skins, robots could perform more dynamic and variable tasks such as rescue missions or caring for the elderly. In this paper, we present a new concept of utilizing a very simple, highly flexible and stretchable capacitor sensor array, that can be attached on the surface of a retractable robot hand to realize three functions: determining the location, shape, and pressure of an object. This adaptive sensing system is accomplished using capacitors connected by aligned carbon nanotube(CNT) films constructed on an elastomer dielectric material, which can reduce the requirement on the accuracy of the machine vision system. This study has a very broad application in the manufacture of intelligent software robots.

关键词： sensor array stretchable location shape pressure

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systematic adsorption process of petroleum hydrocarbon by immobilised petroleum-degradation bacteria system in degradation pathways

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Petroleum Science 2021年第5期18卷 1543-1550页

作者： Xin-Ge Fu Ke Shi Jian-Liang Xue Chuan Chen Yu Bai Yan-Lu Qiao Yi-Xuan Liu Xiang-Ming Hu Yu Gao Hao Yu College of Safety and Environmental Engineering Shandong University of Science and TechnologyQingdaoShandong266590China School of Environment Harbin Institute of TechnologyHaerbinHeilongjiang150001China Chinaunicom system integration Co LtdNo.131Xidan North RoadBeijing100085China College of Chemical and Biological Engineering Shandong University of Science and TechnologyQingdaoShandong266590China

In order to effectively control petroleum hydrocarbon pollution by immobilisation technology,it is necessary to understanding the degradation pathways of petroleum hydrocarbon in immobilised petroleum-degradation bacteria ***,the adsorption in degradation process is rarely *** this study,adsorption process of petroleum hydrocarbon was synergistically studied by means of the surface properties,adsorption thermodynamics and molecular *** results indicate that the immobilised petroleum-degradation bacteria have many holes for the bacteria to *** diesel adsorption by immobilised petroleum-degradation bacteria is a spontaneous,entropy-increasing and endothermic *** is first adsorbed to the surface of immobilised petroleum-degradation bacteria through hydrogen bonding,and then is *** study provided substantial knowledge of immobilised technology in controlling petroleum hydrocarbon pollution.

关键词： Petroleum hydrocarbon pollution Immobilisation Adsorption-biodegradation Molecular simulation Bioremediation

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Iterative multi-photon adaptive compensation technique for deep tissue two-photon fluorescence lifetime imaging

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Chinese Optics Letters 2024年第4期22卷 89-94页

作者：王柯欣余文慧屈军乐廖常锐王义平何俊刘丽炜 State Key Laboratory of Radio Frequency Heterogeneous integration(Shenzhen University) Shenzhen 518060China Key Laboratory of Optoelectronic Devices and systems of Guangdong province&Ministry of Education College of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen 518060China

Fluorescence lifetime imaging can reveal the high-resolution structure of various biophysical and chemical parameters in a microenvironment ***,the depth of imaging is generally limited to hundreds of micrometers due to aberration and light scattering in biological *** paper introduces an iterative multi-photon adaptive compensation technique(IMPACT)into a two-photon fluorescence lifetime microscopy system to successfully overcome aberrations and multiple scattering problems in deep *** shows that 400 correction modes can be achieved within 5 min,which was mainly limited by the frame rate of a spatial light *** system was used for high-resolution imaging of mice brain tissue and live zebrafish,further verifying its superior performance in imaging quality and photon accumulation speed.

关键词： adaptive optics iterative optimization two-photon fluorescence lifetime imaging microscopy wavefront correction

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A neurovascular unit-on-a-chip:culture and differentiation of human neural stem cells in a three-dimensional microfluidic environment

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Neural Regeneration Research 2022年第10期17卷 2260-2266页

作者： Wen-Juan Wei Ya-Chen Wang Xin Guan Wei-Gong Chen Jing Liu Stem Cell Clinical Research Center National Joint Engineering LaboratoryRegenerative Medicine Centerthe First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning ProvinceChina Dalian innovation Institute of Stem Cell and Precision Medicine DalianLiaoning ProvinceChina

Biological studies typically rely on a simple monolayer cell culture,which does not reflect the complex functional characteristics of human tissues and organs,or their real response to external *** technology has advantages of high-throughput screening,accurate control of the fluid velocity,low cell consumption,long-term culture,and high *** combining the multipotential differentiation of neural stem cells with high throughput and the integrated characteristics of microfluidic technology,an in vitro model of a functionalized neurovascular unit was established using human neural stem cell-derived neurons,astrocytes,oligodendrocytes,and a functional microvascular *** model comprises a multi-layer vertical neural module and vascular module,both of which were connected with a syringe *** provides controllable conditions for cell inoculation and nutrient supply,and simultaneously simulates the process of ischemic/hypoxic injury and the process of inflammatory factors in the circulatory system passing through the blood-brain barrier and then acting on the nerve tissue in the *** in vitro functionalized neurovascular unit model will be conducive to central nervous system disease research,drug screening,and new drug development.

关键词： (neural)differentiation astrocyte blood-brain barrier brain microvascular endothelial cells central nervous system microfluidics neural stem cells neuron neurovascular unit oligodendrocyte organ-on-a-chip

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Control of defensive behavior by the nucleus of Darkschewitsch GABAergic neurons

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National Science Review 2024年第4期11卷 70-83页

作者： Huiying Zhao Jinrong Liu Yujin Shao Xiang Feng Binhan Zhao Li Sun Yijun Liu Linghui Zeng Xiao-ming Li Hongbin Yang Shumin Duan Yan-qin Yu Department of Neurology of Second Affiliated Hospital and School of Brain Science and Brain Medicine Zhejiang University School of Medicine Nanhu Brain-Computer Interface Institute Liangzhu Laboratory MOE Frontier Science Center for Brain Science and Brain-Machine Integration State Key Laboratory of Brain-Machine Intelligence Zhejiang University NHC and CAMS Key Laboratory of Medical Neurobiology Zhejiang University Key Laboratory of Novel Targets and Drug Study for Neural Repair of Zhejiang province School of Medicine Hangzhou City University

The nucleus of Darkschewitsch(ND), mainly composed of GABAergic neurons, is widely recognized as a component of the eye-movement controlling system. However, the functional contribution of ND GABAergic neurons(NDGABA) in animal behavior is largely unknown. Here, we show that NDGABA neurons were selectively activated by different types of fear stimuli, such as predator odor and foot *** and chemogenetic manipulations revealed that NDGABA neurons mediate freezing ***, using circuit-based optogenetic and neuroanatomical tracing methods, we identified an excitatory pathway from the lateral periaqueductal gray(l PAG) to the ND that induces freezing by exciting ND inhibitory outputs to the motor-related gigantocellular reticular nucleus, ventral part(Gi V). Together,these findings indicate the NDGABA population as a novel hub for controlling defensive response by relaying fearful information from the l PAG to Gi V, a mechanism critical for understanding how the freezing behavior is encoded in the mammalian brain.

关键词： nucleus of Darkschewitsch GABAergic population defensive behavior freezing neural circuitry

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De novo production of LNnT by metabolic engineering of Saccharomyces cerevisiae as cell factory

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Food Science and Human Wellness 2024年

作者： Han Liu Yingjie Liu Haitao Hu Chenlu Zhu Shuang Zhang Xiaomei Lü Ruijin Yang State Key Laboratory of Food Science and Resources， Jiangnan University School of Food Science and Technology， Jiangnan University Collaborative innovation Center of Food Safety and Quality Control in jiangsu province， Jiangnan University

Lacto-N-neotetraose (LNnT) is a crucial neutral core human milk oligosaccharide (HMO). In this study， we established a LNnT-producing Saccharomyces cerevisiae cell factory was established through comprehensive metabolic engineering. Specifically， the de novo biosynthetic pathway of LNnT was assembled by heterologously expressing the lactose permease (Lac12) from Kluyveromyces lactis and the glycosyltransferase from Neisseria meningitidis in Saccharomyces cerevisiae. Subsequently， carbon source regulation based on the glucose-sensitive GAL regulatory system was employed to optimize the expression time of heterologous genes， achieving a production of 15.61 mg/L of LNnT in shake-flask fermentation. In addition， the key rate-limiting steps involved in LNnT synthesis pathway were identified and the corresponding genes were overexpressed to enhance LNnT production， resulting in an eight-fold increase in LNnT titer compared to that of parental strain. To our knowledge， this is the first report on LNnT biosynthesis in Saccharomyces cerevisiae， opening up the possibility of green production of LNnT using food-safe microorganisms.

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A DEM upscaling method with integrating valley lines based on HASM

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Frontiers of Earth Science 2024年第3期18卷 509-525页

作者： Mingwei ZHAO Xiaoxiao JU Na ZHAO Chun WANG Yan XU Xiaoran WU Weitao LI Geographic Information and Tourism College Chuzhou UniversityChuzhou 239000China College of Resource Environment and TourismCapital Normal UniversityBeijing 100048China State Key Laboratory of Resources and Environmental Information system Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China College of Resources and Environment University of Chinese Academy of SciencesBeijing 100101China Anhui Engineering Laboratory of Geo-information Smart Sensing and Services Chuzhou 239000China Anhui Center for Collaborative innovation in Geographical Information integration and Application Chuzhou 239000China School of Geographic Information and Tourism Chuzhou UniversityChuzhou 239000China

A new digital elevation model(DEM)upscaling method based on high accuracy surface modeling(HASM)is proposed by combining the elevation information of DEM and the valley lines extracted from DEM with different flow accumulation *** proposed method has several advantages over traditional DEM upscaling ***,the HASM ensures the smoothness of the upscaled ***,several DEMs with different topographic details can be obtained using the same DEM grid size by incorporating the valley lines with different flow accumulation *** Jiuyuangou watershed in China’s Loess Plateau was used as a case study.A DEM with a grid size of 5 m obtained from the local surveying and mapping department was used to verify the proposed DEM upscaling *** established the surface complexity index to describe the complexity of the topographic surface and quantified the differences in the topographic features obtained from different upscaling *** results show that topography becomes more generalized as grid size and flow accumulation threshold *** a large DEM grid size,an increase in the flow accumulation threshold increases the difference in elevation values in different grids,increasing the surface complexity *** study provides a new DEM upscaling method suitable for quantifying topography.

关键词： DEM upscaling HASM flow accumulation threshold surface complexity index

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Detection of Huanglongbing(citrus greening) based on hyperspectral image analysis and PCR

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Frontiers of Agricultural Science and Engineering 2019年第2期6卷 172-180页

作者： Kejian WANG Dongmei GUO Yao ZHANG Lie DENG Rangjin XIE Qiang LV Shilai YI Yongqiang ZHENG Yanyan MA Shaolan HE Southwest University/Citrus Research Institute of Chinese Academy of Agricultural Sciences Chongqing 400712 China National Agricultural Technology Extension and Service Center Beijing 100125 China Chengdu Plant Quarantine Station Chengdu 610000 China Key Laboratory of Modern Precision Agriculture system integration Research China Agricultural University Beijing 100083 China

Huanglongbing (HLB, citrus greening) is one of the most serious quarantine diseases of citrus worldwide. To monitor in real-time, recognize diseased trees, and efficiently prevent and control HLB disease in citrus, it is necessary to develop a rapid diagnostic method to detect HLB infected plants without symptoms. This study used Newhall navel orange plants as the research subject, and collected normal color leaf samples and chlorotic leaf samples from a healthy orchard and an HLB-infected orchard, respectively. First, hyperspectral data of the upper and lower leaf surfaces were obtained, and then the polymerase chain reaction (PCR) was used to detect the HLB bacterium in each leaf. The PCR test results showed that all samples from the healthy orchard were negative, and a portion of the samples from the infected orchard were positive. According to these results, the leaf samples from the orchards were divided into disease-free leaves and HLB-positive leaves, and the least squares support vector machine recognition model was established based on the leaf hyperspectral reflectance. The effect on the model of the spectra obtained from the upper and lower leaf surfaces was investigated and different pretreatment methods were compared and analyzed. It was observed that the HLB recognition rate values of the calibration and validation sets based on upper leaf surface spectra under 9-point smoothing pretreatment were 100% and 92.5%, respectively. The recognition rate values based on lower leaf surface spectra under the second-order derivative pretreatment were also 100% and 92.5%, respectively. Both upper and lower leaf surface spectra were available for recognition of HLB-infected leaves, and the HLB PCR-positive leaves could be distinguished from the healthy by the hyperspectral modeling analysis. The results of this study show that early and nondestructive detection of HLBinfected leaves without symptoms is possible, which provides a basis for the hyperspectral diagnosi

关键词： citrus HLB hyperspectral identification PCR

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