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Studies of a Cerco.pora Nico.ianae Strain on Tobacco.in China

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当代农业（中英文版） 2016年第1期5卷 1-7页

作者： Fangfang Li Wei Liu Na Sun Fenglong Wang Lili Shen Tobacco.Research Institute Chinese Academy of Agricultural Science Qingdao 266101 China Shandong Hongri Acron chemical Joint Stock co. Ltd Linyi China

Effects of Aschersonia aleyrodis on the life table and demographic parameters of Bemisia tabaci

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Journal of Integrative Agriculture 2018年第2期17卷 389-396页

作者： ZHANG Can SHAO Zhen-fang HAN Yue-ye WANG Xing-min WANG Ze-qing Peter Dennis Musa QIU Bao-li Shaukat Ali Key Laboratory of Bio-pesticide Innovation and Application of Guangdong Province Department of Entomology South China Agricultural University Guangzhou 510642 P.R.China Guangdong Engineering Research Centre of Microbial Pesticides Guangdong New Scene Biological Engineering Co. Ltd. Yangjiang 529932 P.R.China Huai'an Entry-Exit Inspection & Quarantine Bureau Huai'an 223001 P.R. China

The present study reports the sublethal effects of the entomopathogenic fungus, Aschersonia aleyrodis（Webber） on Bemisia tabaci（Gennadius）（Homoptera： Aleyrodidae）. A fungal suspension of A. aleyrodis isolate Aa005 co.taining 1×107 co.idia mL^-1 was sprayed against B. tabaci on eggplant leaves under greenhouse co.ditions. The effects of fungal application on survival as well as life table parameters of the whitefly were observed at different post inoculation periods. The results indicated that A. aleyrodis can significantly affect the survival of 1st, 2nd, and 3rd nymphal instars of B. tabaci. Developmental periods of different instar nymphs were not affected by fungal application. A. aleyrodis spores persisted well and significantly affected the survivorship of young instar nymphs of B. tabaci at different post incubation periods. Life table results suggested that A. aleyrodis has no impact on general fecundity and longevity of B. tabaci. When the pathogen was exposed to the open environment and maintained for a relatively longer period, a reduction in the reproductive rate and intrinsic rate of increase was caused by the fungal spores, though the sublethal effects were not as good as the co.trol treatment. The results suggest that the ability of spores to suppress an increase in whitefly population persists well after incubation of spores to the external environment（up to 9 days）.

关键词： entomopathogenic fungi Aschersonia aleyrodis Bemisia tabaci life table

Roles of the acyl-co. Dehydrogenase Family in Fusarium Sacchari

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Roles of the acyl-CoA Dehydrogenase Family in Fusarium Sacch...

中国植物病理学会2024年学术年会

作者： ZENG Quan YU Quan MO Yingxi LIANG Haoming CHEN Baoshan MENG Jiaorong State Key Laboratory for co.servation and Utilization of Subtropical Agro-Bioresources and Ministry and Province co.sponsored co.laborative Innovation Center for Sugarcane and Sugar industry Guangxi University co.lege of Life Science and Technology Guangxi University Guangxi Key Laboratory of Sugarcane Biology and Academy of Sugarcane and Sugar industry College of AgricultureGuangxi University

Fusarium sacchari is one of the causal agents of Pokkah boeng,an important disease of sugarcane *** acyl-co. dehydrogenases(ACADs) are a family of flavoenzymes involved in the β-oxidation of fatty acid and amino acid catabolism in ***,little is known about the role of the ACADs in the pathogenesis of ***,and there was no systemic report on the gene family *** prior study,a mutant library of *** was generated,and subsequent pathogenicity evaluations led to the disco.ery of a mutant harboring an insertion in the FsACAD gene,exhibiting diminished *** this study,a total of 14 ACAD-enco.ing genes(FsACAD-1 through FsACAD-14) were identified by screening the whole genome sequence of *** FsACAD genes were found to distribute across seven chromosomes and co.ld be classified into six clades by phylogenetic analysis of the protein *** vivo mRNA quantification unveiled that FsACAD genes expressed differentially during infection of the *** expression patterns of FsACAD genes also differed significantly in response to the induction of different class of fatty ***,fatty acid utilization assays of the FsACAD-deletion mutants revealed that different ACADs varied in their preference and ability to breakdown the fatty acids with different carbon chain *** a result,different FsACAD-deletion mutants exhibited varied degree of adverse impact on the biological traits of the fungus,including growth,co.idiation,tolerance to stress,and *** findings provide in insights into the co.prehensive roles of the FsACADs in *** and may facilitate the future effort to identify key FsACAD targets for better co.trol of Pokkah boeng disease.

关键词： Fusarium sacchari Pokkah boeng disease ACAD fatty acid β-oxidation

Using Rn-222 to Study Human-Regulated River Water-Sediment Input Event in the Estuary

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Journal of Ocean University of China 2023年第6期22卷 1464-1472页

作者： XU Yuezhi XIAO Liu GAO Maosheng YANG Disong ZHAO Shibin XU Haowei WANG lisha ZHANG Xiaojie co.lege of Chemistry and Chemical Engineering Ocean University of ChinaQingdao 266100China Key Laboratory of Marine Chemistry Theory and Technology Ministry of Education and Frontiers Science Center for Deep Ocean Multispheres and Earth SystemOcean University of ChinaQingdao 266100China SGS-CSTC Standard Technical Service(Qingdao)co. Ltd.Qingdao 266100China Qingdao Institute of Marine Geology China Geological SurveyQingdao 266071China

The implementation of the water sediment regulation scheme(WSRS)is a typical example of artificially co.trolling land-source *** WSRS,the water discharge of the Yellow River will increase significantly,and so will the input of terri-genous *** this study,we used a natural geochemical tracer 222Rn to quantify terrestrial inputs under the influence of the 2014 WSRS in the Yellow River *** results indicated that during WSRS the co.centration of 222Rn in the estuary increased by about four times than in the period before *** high-level 222Rn plume disappeared quickly after WSRS,indicating that 222Rn has a very short‘memory effect’in the *** on the investigation co.ducted from 2015 to 2016,the co.centration of 222Rn tended to be stable in the lower reaches of the Yellow *** WSRS,the co.centrations of 222Rn in the river water in-creased sharply at about 3–5 times greater than in the non-WSRS *** on the 222Rn mass balance model,the fluxes of 222Rn caused by submarine groundwater discharge(SGD)were estimated to be(3.5±1.7)×10^(3),(11±3.9)×10^(3),and(5.2±1.9)×10^(3)dpm m^(-2)d^(-1)in the periods before,during,and after WSRS,*** finding indicated that SGD was the major source of 222Rn in the Yellow River Estuary,which can be significantly increased during ***,the SGD-associated nutrient fluxes were estimated to be 9.8×10^(3),2.5×102,and 1.1×10^(4)μmolm^(-2)d^(-1)for dissolved inorganic nitrogen,phosphorus,and silico.,respectively,during WSRS or about 2–40 times greater than during the non-WSRS period.

关键词： 222Rn submarine groundwater discharge water sediment regulation scheme nutrient Yellow River

Integrated transcriptome and metabolome analysis of Oryza sative *** infection with Rhizoctonia solani,the causal agent of rice sheath blight

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Integrated transcriptome and metabolome analysis of Oryza sa...

江苏省遗传学会2016年学术年会

作者： Wenlei Cao Bin Xu Limin Yuan Xijun Chen Xuebiao Pan Shimin Zuo jiangsu Key Laboratory of Crop Genetics and Physiology/co.Innovation Center for Modern Production Technology of Grain Crops Yangzhou University Testing center of Yangzhou University

Sheath blight（SB）,caused by necrotrophic fungus Rhizoctonia solani Kühn（***）,gives rise to severely grain losses in *** various resistance levels of rice varieties to SB have been reported,the underlying resistance mechanism remains largely unknown due to lack of enough information on candidate genes/*** this study,an integrated approach was employed to try to unco.er potential mechanism of rice resistance to *** reveal the molecular mechanism of riceresponse to *** and mine the candidate genes/pathways regulating rice sheath blight（SB） resistance through transcription and metabolite profiling and genetic ***1 is a novel germplasm with stable and high resistance to SB,which has been identified by using greenhouse and field *** analysis by SEM（scanning electronic microsco.y） showed that the growth of *** mycelium was apparently suppressed in YSBR1 but not in the susceptible variety Lemont after 60 HAI（hours after inoculation）.*** infection was able to trigger substantial metabolic reprogramming in rice,particularly in primary metabolism and energy *** and tyrosine,which have been co.firmed to involve in plant-pathogen interaction via the biosynthesis of seco.dary metabolites,were specially increased in YSBR1 *** of 332/62（Lemont/YSBR1） and 2606/748（Lemont/YSBR1） DEGs（differential expression genes） at 10 and 20 HAI were identified,*** varieties,24 and 538 DEGs were found with same expression pattern in response to *** 10 and 20 HAI.A total of 247 unique DEGs were identified specifically in YSBR1,which were annotated to involve in several pathways,like amino acid metabolism,seco.dary metabolic,energy metabolism,photosynthesis,and sugar metabolism *** co.bining genetic analysis and omics evidence,a few candidate genes/pathways regulating rice SB resistance were identified,among them 6 genes localized within the known SBR QTL region（Lemont/

关键词： Rhizoctonia solani rice metabolome transcrptome QTLs candidate genes/pathways

Integrated Transcriptome and Metabolome Analysis of Oryza sative *** Infection with Rhizoctonia solani,the Causal Agent of Rice Sheath Blight

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Integrated Transcriptome and Metabolome Analysis of Oryza sa...

中国植物病理学会2017年学术年会

作者： Cao Wenlei Xu Bin Yuan Limin Chen Xijun Pan Xuebiao Zuo Shimin jiangsu Key Laboratory of Crop Genetics and Physiology/co.Innovation Center for Modern Production Technology of Grain Crops Yangzhou University Testing Center of Yangzhou University

Sheath blight（SB）,caused by necrotrophic fungus Rhizoctonia solani Kiihn（***）,gives rise to severely grain losses in *** this study,an integrated approach of transcriptomics and metabolomics was employed to try to unco.er potential mechanism and to mine the candidate genes/pathways regulating rice SB *** is a novel germplasm with stable and high resistance to SB,which has been identified by using greenhouse and field *** analysis by SEM showed that the growth of *** mycelium was apparently suppressed in YSBRl but not in Lemont（susceptible） after 60 HAL *** infection was able to trigger substantialmetabolic reprogramming in rice,particularly in primary metabolismand energy *** and tyrosine,which have been co.firmed to involve in plant-pathogen interaction via the biosynthesis of seco.dary metabolites,were specially increased in YSBRl *** of 332/62（Lemont/YSBR1） and 2606/748（Lemont/YSBRl） DEGsat 10 and 20 HAI were identified,respectively.A total of 247 unique DEGs were identified specifically in YSBR1,which were annotated to involve in several pathways,like amino acid metabolism,seco.dary metabolic,energy metabolism,photosynthesis,and sugar metabolism *** co.bining genetic analysis and omics evidence,6 candidate genes localized within the known SBR QTL region（Lemont/YSBR1）,enco.ing cellulose synthase-like family A,transcription factors TF Ⅱ S,elongin A,EF hand family protein,calmodulin-related calcium sensor protein,ERF transcription factor,and thaumatin,have been identified.

关键词： Rhizoctonia solani Rice Metabolome transcrptome QTLs Candidate genes/pathways

Green synthesis of multifunctional carbon dots for anti-cancer and anti-fungal applications

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Chinese Journal of Chemical Engineering 2021年第9期34卷 97-104页

作者： Shaojing Zhao Li Huang Yong Xie Bin Wang Feng Wang Minhuan Lan Hunan Provincial Key Laboratory of Micro&Nano Materials Interface Science College of Chemistry and Chemical EngineeringCentral South UniversityChangsha 410083China State Key Laboratory of the Disco.ery and Development of Novel Pesticide Shenyang Sinochem Agrochemicals R&D Co.Ltd.Shenyang 110021China Key Laboratory of Materials Chemistry for Energy co.version and Storage of Ministry of Education Hubei Key Laboratory of Material Chemistry and Service FailureSchool of Chemistry and Chemical EngineeringHuazhong University of Science and TechnologyWuhan 430074China

Carbon dots(CDs)have beco.e popular nanomaterials in biomedical and agricultural *** we synthesized multifunctional CDs which showed anti-cancer and anti-fungal *** low cytotoxicity,stable fluorescence and high photothermal co.version efficiency enable the CDs with imagingguided photothermal *** CDs also exhibited intrinsic anti-fungal activity even at a low co.centration,i.e.,40 mg·L^(-1) of CDs induced 20%mortality in cucumber downy ***,the largeπ-co.jugated nanostructure and the richness of amino and hydroxyl groups make them a powerful delivery platform for flumorph(a fungicide)with a high loading efficiency of 47.18%.Meanwhile,the heat co.verted from the light can accelerate the release of flumorph from CDs,and thus efficiently kill fungus.

关键词： Carbon dots Photothermal therapy Anti-fungus Delivery Pesticide

Integrated transcriptome and metabolome analysis on resistant and susceptible varieties to Rhizoctonia solani provide new insights to understanding rice resistance mechanism to sheath blight

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Integrated transcriptome and metabolome analysis on resistan...

2018中国作物学会学术年会

作者：曹文磊 Zhang Hui-Min Xu Bin Yuan Limin Guo Min David De Vleesschauwer Zhao JianHua Feng Zhiming Chen Xi-Jun Chen Zongxiang Zhang Yafang Pan Xue-Biao Yan Changjie Qiaoquan Liu Zuo Shi-Min jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/jiangsu Key Laboratory of Crop Genomics and Molecular Breeding Agricultural College of Yangzhou University jiangsu co.Innovation Center for Modern Production Technology of Grain Crops Yangzhou University co.lege of horticulture and plant protection at Yangzhou University Testing center of Yangzhou University Laboratory of Phytopathology Faculty of Bioscience EngineeringGhent University

[Background]Sheath blight(SB),caused by necrotrophic fungus Rhizoctonia solani Kuhn(***),gives rise to devastating yield losses in ***,due to quantitative resistance characteristics of rice to SB,the resistant mechanism of rice to this disease remains largely unknown,and which leads to a slowly progress on developing resistant varieties.[Materials and Methods]The isolate YN-7 with moderate pathogenicity on rice was used to inoculate rice varieties YSBR1(resistant) and Lemont(susceptible) in both greenhouse and field *** SB resistance levels were evaluated acco.ding to the description in Zuo et al.(2014).The differences of mycelium morphology after inoculation in two varieties were investigated by SEM(scanning electronic microsco.y).Metabolites from the sampled leave sheaths of both varieties at 40 HAI(hours after inoculation) with and without *** were extracted and measured based on GC/*** samples extracted from the same part of the two varieties as that in the metabolic analysis at 10 and 20 HAI were used to perform microarray assay.[Result and Analysis]In the present study,we found that rice variety YSBR1 shows stably high resistance to *** in both greenhouse and field co.ditions,and displaying an apparently ability to suppress the mycelium growth of *** and the formation of infection *** better understand the molecular mechanism of rice against ***,we used global transcriptional and metabolomics analyses to investigate the changes in gene expression and metabolic processes in YSBR1 and Lemont(a susceptible variety)upon *** *** and metabolic profiling data displayed 2429/714 differentially expressed genes(DEGs) and 76/90 differentially accumulated metabolites(DEMs) in Lemont/YSBRl,*** the co.bination analysis,we suggested that two kinds of responses(resistant and susceptible) to *** infection in rice were the determinant of rice phenotype to SB *** r

关键词： rice sheath blight disease omics,resistant response,susceptible response

Evolutionary biogeography on Ophioco.dyceps sinensis:An indicator of molecular phylogeny to geochronological and eco.ogical exchanges

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Geoscience Frontiers 2020年第3期11卷 807-820页

作者： Yongdong Dai Changkui Wu Feng Yuan Yuanbing Wang Luodong Huang Zihong Chen Wenbo Zeng Yao Wang Zhuliang Yang Pusheng Zeng Paul Lemetti Xuanxue Mo Hong Yu Yunnan Herbal Laboratory Institute of Herb Biotic ResourcesSchool of Life SciencesYunnan UniversityKunmingYunnan Province 650091China Yunnan Herbal Biotech co. LtdKunmingYunnan Province 650203China Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunmingYunnan Province 650201China National Research Center for Geoanalysis Beijing 100037China School of Earth Sciences and Resources China University of Geosciences(Beijing)Beijing 100083China

The fungus Ophioco.dyceps sinensis is endemic to the vast region of the Qinghai-Tibetan plateau(QTP).The unique and co.plex geographical environmental co.ditions have led to the "sky island" distribution structure of *** to limited and unbalanced sample co.lections,the previous data on *** regarding its genetic diversity and spatial structure have been deemed *** this study,we analyzed the diversity and phylogeographic structures of *** using internally transcribed spacer region(ITS) and 5-locus datasets by a large-scale sampling.A total of 111 haplotypes of ITS sequences were identified from 948 samples data of the fungus ***,with representing high genetic diversity,and 8 phylogenetic clades were reco.nized in *** the southeastern Tibet and the northwestern Yunnan were the centers of genetic diversity and genetic differentiation of the fungus,and they were inferred as the glacial refugia in the *** distribution patterns were identified to co.respond to the 8 clades,including but not limited to the co.xistence of widely and specific local distributive *** also revealed that the differentiation pattern of *** did not fit for the isolation-by-distance *** differentiation into the 8 clades occurred between 1.56 Myr and6.62 *** ancestor of *** most likely originated in the late Miocene(6.62 Myr) in the northwestern Yunnan,and the Scene A-C of the Qinghai-Tibetan movements may have played an important role in the differentiation of *** during the late Miocene-Pliocene *** current results provide a much clearer and detailed understanding of the genetic diversity and geographical spatial distribution of the endemic alpine fungus *** also revealed that the geochronology resulting from paleogeology co.ld be cross-examined with biomolecular clock at a finer scale.

关键词： Large-scale sampling Phylogeographic structure Molecular clock co.relation Qinghai-Tibetan plateau