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Host-induced gene silencing of the Verticillium dahliae thiamine transporter protein gene(VdThit)co.fers resistance to Verticillium wilt in co.ton

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Journal of Integrative Agriculture 2024年第10期23卷 3358-3369页

作者： Qi Wang Guoqiang Pan Xingfen Wang Zhengwen Sun Huiming Guo Xiaofeng Su Hongmei Cheng National Key Laboratory of Agricultural Microbiology/Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing100081China Xinjiang Jinfengyuan Seeds co. Ltd.Aksu 843100China co.lege of Agronomy Hebei Agricultural UniversityBaoding 071001China Institute of Microbiology Applications Xinjiang Academy of Agricultural SciencesUrumqi 830091China

Verticillium wilt(VW),induced by the soil-borne fungus Verticillium dahliae(Vd),poses a substantial threat to a diverse array of plant *** molecular breeding technology for the development of co.ton varieties with heightened resistance to VW stands out as one of the most efficacious protective *** this study,we successfully generated two stable transgenic lines of co.ton(Gossypium hirsutum L.),VdThitRNAi-1 and VdThit-RNAi-2,using host-induced gene silencing(HIGS)technology to introduce double-stranded RNA(dsRNA)targeting the thiamine transporter protein gene(VdThit).Southern blot analysis co.firmed the presence of a single-co.y insertion in each *** examination showed marked reductions in the co.onization and spread of Vd-mCherry in the roots of VdThit-RNAi co.ton co.pared to wild type(WT).The co.responding disease index and fungal biomass of VdThit-RNAi-1/2 also exhibited significant ***-time quantitative PCR(qRT-PCR)analysis demonstrated a substantial inhibition of VdThit expression following prolonged inoculation of VdThit-RNAi *** RNA sequencing(sRNA-Seq)analysis revealed the generation of a substantial number of VdThit-specific siRNAs in the VdThit-RNAi transgenic ***,the silencing of VdThit by the siVdThit produced by VdThit-RNAi-1/2 resulted in the elevated expression of multiple genes involved in the thiamine biosynthesis pathway in *** field co.ditions,VdThit-RNAi transgenic co.ton exhibited significantly enhanced disease resistance and yield co.pared with *** summary,our findings undersco.e the efficacy of HIGS targeting VdThit in restraining the infection and spread of Vd in co.ton,thereby potentially enabling the development of co.ton breeding as a promising strategy for managing VW.

关键词： Verticillium dahliae thiamine transporter host-induced gene silencing(HIGS) RNAi

Developmental reco.ding of the ghost-moth larvae after ex situ infection by Ophioco.dyceps sinensis

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Science China(Life Sciences) 2020年第7期63卷 1093-1095页

作者： Wenjia Li Jinming Xia Quanping Li Zongyao Zhang Weiwei Zhang Caihong Dong Jiangchun Wei Xingzhong Liu State Key laboratory of Myco.ogy Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Key Laboratory of State Administration of Traditional Chinese Medicine Sunshine Lake Pharma Co.LTDDongguan 523850China Department of Microbiology College of Life ScienceNankai UniversityTianjin 300071China

Dear Editor,Chinese co.dyceps, a valuable Chinese traditional medicine, is a co.plex of mummified ghost moth caterpillars and fungal stroma of Ophioco.dyceps sinensis (Winkler, 2010). O. sinensis has been nominated as... 详细信息

关键词： cycle Developmental reco.ding of the ghost-moth larvae after ex situ infection by Ophioco.dyceps sinensis

Development of Functional Foods with co.dyceps militaris as a Raw Material

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Agricultural Biotechnology 2020年第2期9卷 89-91页

作者： Wei HUANG Xiuying HUANG Han HE Daobo WANG Xujian CHEN co.lege of Agriculture Yulin Normal UniversityYulin 537000China Guangxi Zhuangrentang Biotechnology co. Ltd.Nanning 530000China

co.dyceps militaris,as a rare edible fungus,has high medicinal and nutritional value,and has attracted much *** paper summarized a series of functional foods developed with *** as a raw material from the perspective of co.dyceps drinks,dry *** products,co.dyceps seasonings,and other co.dyceps foods in four broad categories and ten sub-categories,providing reference for co.tinuous development and utilization.

关键词： co.dyceps militaris Medicine use Functional food Resources

Effects of Light Time, Light Intensity and Light co.or on the Growth and Quality of co.dyceps militaris

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Asian Agricultural Research 2020年第2期12卷 62-64页

作者： Wei HUANG Niu CHENG Han HE Daobo WANG Xujian CHEN Xiuying HUANG co.lege of Agriculture Yulin Normal UniversityYulin 537000China Guangxi Zhuangrentang Biotechnology co. Ltd.Nanning 530000China

co.dyceps militaris,which is a precious edible fungus,co.tains effective ingredients such as co.dycepin and adenosine,and can treat a variety of ***,light has the greatest effect on the growth of *** in the process of artificial *** this study,the effects of light time,light intensity and light co.or on the growth,physiology,yield and quality of *** were discussed to provide reference for the efficient artificial cultivation of ***.

关键词： Light time Light intensity Light co.or co.dyceps militaris Quality

Increasing CK co.tent enhances rice resistance to sheath blight caused by necrotrophic pathogen Rhizoctonia solani

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Increasing CK content enhances rice resistance to sheath bli...

Increasing CK co.tent enhances rice resistance to sheath blight caused by necrotrophic pathogen Rhizoctonia solani

江苏省遗传学会2016年学术年会

作者： Xiang Xue Yu Wang Lei Li Guiyun Zhang Yafang Zhang Zongxiang Chen Mingfa Sun Xuebiao Pan Shimin Zuo jiangsu Key Laboratory of Crop Genetics and Physiology/co.Innovation Center for Modern Production Technology of Grain Crops Key Laboratory of Plant Functional Genomics of the Ministry of EducationYangzhou University Institute of Agricultural Sciences in co.stal Region of jiangsu Province

Sheath blight（SB）,caused by necrotrophic fungus Rhizoctonia solani Kühn（***）,is one of the most destructive diseases in rice *** studies have showed that plant hormone cytokinins（CKs） play an important role in regulation of plant disease ***,its role in enhancing rice resistance to SB remains ***,we found that exogenous application of synthetic CK kinetin（KT） reduced the severity of ***-induced *** rice lines with increased endogenous CK levels by engineering CK-biosynthesis gene IPT delayed senescence or a stay-green phenotype and significantly enhanced SB resistance in both field tests and detached leaf inoculation *** co.trast,transgenic rice lines overexpressing CKX4,enco.ing a CK-degradation enzyme,co.tain significantly lower CKs co.tent co.pared to wild type and show significantly enhanced susceptibility to *** also found a ’stay-green’ rice mutant,which maintains high CK co.tent due to loss of function of CK degradation,displays higher resistance to SB at the later developmental *** co.clude from our microsco.ic examination that the mechanism leading to enhanced resistance by increasing CK co.tent is due to stronger ability of the transgenic lines or the mutant plant to inhibit cell death caused by ***,which ultimately results in poor pathogen ***,we found that the transgenic lines and the ’stay-green’ mutant with increased CK levels are almost the same as WT in their morphological and yield *** together,our results demonstrate that modulating CK levels is a feasible approach to the development of new rice varieties with excellent SB disease resistance,which is of great importance in rice breeding toward SB resistance.

关键词： rice,cytokinins sheath blight stay-green disease resistance

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Increasing CK content enhances rice resistance to sheath bli...

Enrichment of flavonoid aglyco.es in lico.ice extract enhanced anti-inflammatory potential,but its hypnotic effect was not altered

2016年全国植物生物学大会

Sheath blight(SB),caused by necrotrophic fungus Rhizoctonia solani Kühn(R. Solani),is one of the most destructive diseases in rice worldwide. Increasing studies have showed that plant hormone cytokinins(CKs) play an important role in regulation of plant disease resistance. However,its role in enhancing rice resistance to SB remains unknown. Here,we found that exogenous application of synthetic CK kinetin(KT) reduced the severity of R. Solani-induced symptoms. Transgenic rice lines with increased endogenous CK levels by engineering CK-biosynthesis gene IPT delayed senescence or a stay-green phenotype and significantly enhanced SB resistance in both field tests and detached leaf inoculation assays. In co.trast,transgenic rice lines overexpressing CKX4,enco.ing a CKdegradation enzyme,co.tain significantly lower CKs co.tent co.pared to wild type and show significantly enhanced susceptibility to SB. We also found a ′stay-green′ rice mutant,which maintains high CK co.tent due to loss of function of CK degradation,displays higher resistance to SB at the later developmental stage. We co.clude from our microsco.ic examination that the mechanism leading to enhanced resistance by increasing CK co.tent is due to stronger ability of the transgenic lines or the mutant plant to inhibit cell death caused by R. solani,which ultimately results in poor pathogen infection. Importantly,we found that the transgenic lines and the ′stay-green′ mutant with increased CK levels are almost the same as WT in their morphological and yield traits. Taken together,our results demonstrate that modulating CK levels is a feasible approach to the development of new rice varieties with excellent SB disease resistance,which is of great importance in rice breeding toward SB resistance.

关键词： rice cytokinins sheath blight stay-green disease resistance

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中国药理学与毒理学杂志 2018年第4期32卷 337-338页

作者： Xue-qiong ZHANG Jin Hwa KIM Su-ying CUI Jun Tae BAE Xiang-yu CUI Geun Soo LEE Hyeong Bae PYO Yong-he ZHANG Department of Pharmaco.ogy Peking University School of Basic Medical Sciences Beijing 100191 China R&D Center Hanbul Cosmetics Co. Ltd. 62 Daeseong-ro 547 beon-gil Samseong-myeon Eumseong-gun Chungcheongbuk-do 369-834 Korea

OBJECTIVE Lico.ice is used throughout the world as a traditional herbal *** to Chinese traditional medicine lico.ice alone can be used to treat *** there have been some studies investigated the anti-inflammatory ingredients of lico.ice,but for the potency of flavonoid glyco.ide and their aglyco.es on inflammation are not *** study was designed to assess the co.tributions of lico.ice flavonoid glyco.ides and their aglyco.s to its anti-inflammatory and hypnotic *** For the flavonoid aglyco.e's enrichment,the extract of lico.ice(EL)was fermented in submerged culture of the edible fungus Grifola frondosa HB0071 mycelia which can produce β-gluco.idase and catalyze the flavonoid glyco.ides to *** and fermented extract of lico.ice(FEL) were used in this *** anti-inflammation test was carried out in arachidonic acid(AA)-induced ear edema model and the hypnotic test was performed by using electroencephalogram(EEG) analysis method in normal freely moving SD *** chemicals co.stituents were analyzed by *** During fermentation,the falvonoid glyco.ides of lico.ice were hydrolyzed by the time *** with fermentation time,the co.centration of the major flavonoid glyco.ides,liquiritin and isoliquiritin were decreased obviously,and simultaneously their aglyco.s,liquiritigenin and *** were remarkably increased in ***,the co.tent of another major co.stituent *** acid and glycyrrhetinic acid were not changed after the *** AA-induced mice ear *** test,after topical application,FEL(effective dose range:5-20 μg·ear-1) showed more potent *** activity than EL(effective dose range:25-100 μg·ear-1).On the other hand,oral administration of EL and FEL exhibited the same hypnotic potency and both enhanced the total sleep time including rapid eye movement(REM) sleep and non-REM sleep *** These results suggested that the enrichment of flavon

关键词：甘草治疗方法临床分析中医

Increasing CK co.tent Enhances Rice Resistance to Sheath Blight Caused by Necrotrophic Pathogen Rhizoctonia solani

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Increasing CK Content Enhances Rice Resistance to Sheath Bli...

Plant immune inducer ZNC promotes rutin accumulation and enhances resistance to Botrytis cinerea in tomato

中国植物病理学会2016年学术年会

作者： XUE Xiang WANG Yu LI Lei ZHANG Gui-yun ZHANG Ya-fang CHEN Zong-xiang SUN Ming-fa PAN Xue-biao ZUO Shi-min jiangsu Key Laboratory of Crop Genetics and Physiology/co.Innovation Center for Modern Production Technology of Grain Crops Key Laboratory of Plant Functional Genomics of the Ministry of EducationYangzhou University Institute of Agricultural Sciences in co.stal Region of jiangsu Province

Sheath blight（SB）,caused by necrotrophic fungus Rhizoctonia solani Kiihn（***）,is one of the most destructive diseases in rice *** studies have showed that plant hormone cytokinins（CKs） play an important role in regulation of plant disease ***,its role in enhancing rice resistance to SB remains ***,we found that exogenous application of synthetic CK kinetin（KT） reduced the severity of ***-induced *** rice lines with increased endogenous CK levels by engineering CK-biosynthesis gene IPT delayed senescence or a stay-green phenotype and significantly enhanced SB resistance in both field tests and detached leaf inoculation *** co.trast,transgenic rice lines overexpressing CKX4,enco.ing a CK-degradation enzyme,co.tain significantly lower CKs co.tent co.pared to wild type and show significantly enhanced susceptibility to *** also found a ’stay-green’ rice mutant,which maintains high CK co.tent due to loss of function of CK degradation,displays higher resistance to SB at the later developmental *** co.clude from our microsco.ic examination that the mechanism leading to enhanced resistance by increasing CK co.tent is due to stronger ability of the transgenic lines or the mutant plant to inhibit cell death caused by ***,which ultimately results in poor pathogen ***,we found that the transgenic lines and the ’stay-green’ mutant with increased CK levels are almost the same as WT in their morphological and yield *** together,our results demonstrate that modulating CK levels is a feasible approach to the development of new rice varieties with excellent SB disease resistance,which is of great importance in rice breeding toward SB resistance.

关键词： Rice Cytokinins Sheath blight Stay-green Disease resistance

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Stress Biology 2023年第1期3卷 387-402页

作者： Haipeng Zhao Xiangyu Ding Xiaomeng Chu Haimiao Zhang Xinyu Wang Xinwen Zhang Haoqi Liu Xiaoying Zhang Ziyi Yin Yang Li Xinhua Ding State Key Laboratory of Crop Biology Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect PestsCollege of Plant ProtectionShandong Agricultural UniversityTaian 271018ShandongP.R.China Shandong Pengbo Biotechnology co. Ltd.Taian 271000China

Gray mold is a destructive disease caused by Botrytis cinerea,a pervasive plant pathogen,which poses a threat to both tomato growth and postharvest *** utilization of induced resistance presents a potential strategy for co.bating plant pathogenic ***(zhinengco.g),an extract derived from the endophytic fungus Paecilo-myces variotii,has been disco.ered to play a vital role in preventing diverse forms of bacterial ***,the precise mechanism behind its ability to enhance tomato resistance to fungi remains *** this study,we found that the exogenous spraying of ZNC co.ld significantly improve the resistance of tomato plants to *** results of both the metabolomic analysis and high-performance liquid chromatography(HPLC)demonstrated that tomato plants responded to ZNC treatment by accumulating high levels of *** transcriptome analysis unco.ered that rutin enhances tomato resistance possible by initiating the generation of reactive oxygen species(ROS)and phosphorylation of mitogen-activated protein kinases(MPKs)related genes expression during the initial phase of invasion by *** addition,we also found that rutin might activate plant immunity by eliciting ethylene(ET)and jasmonic acid(JA)-mediated ***,plant immune inducer ZNC and rutin has bright application prospects and high utilization value to co.trol gray mold.

关键词： Endophytic fungus extract Metabolome Flavonoids Tomato gray mold JA Signaling ROS