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Introgression of sharp eyespot resistance from Dasypyrum villosum chromosome 2VL into bread wheat

The Crop Journal 2023年第5期11卷 1512-1520页

作者： Caiyun Liu Wei Guo Yang Wang Bisheng Fu Jaroslav Doležel Ying Liu Wenling Zhai Mahmoud Said István Molnár Kateřina Holušová Ruiqi Zhang Jizhong Wu Institute of Germplasm Resources and Biotechnology/jiangsu Provincial Key Laboratory of Agrobiology Jiangsu Academy of Agricultural SciencesNanjing 210014JiangsuChina college of Agronomy/JCIC-MCP/National Key Lab of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095JiangsuChina Institute of Experimental Botany Centre of the HanáRegion for Biotechnological and Agricultural ResearchŠlechtitelů31CZ-783671 Olomoucthe Czech Republic Field Crops research Institute Agricultural Research Centre9 Gamma StreetGiz112619 CairoEgypt agricultural Institute Centre for Agricultural ResearchELKHMartonvásár 2462Hungary jiangsu co-Innovation center for Modern Production technology of Grain Crops Yangzhou UniversityYangzhou 225009JiangsuChina Zhongshan Biological Breeding Laboratory Nanjing 210014JiangsuChina

Wheat sharp eyespot, a stem disease caused by the soilborne fungus Rhizoctonia cerealis van der Hoeven,has become a threat to wheat production worldwide. Exploiting resistance resources from wild relatives of wheat is a promising strategy for controlling this disease. In this study, a new wheat–Dasypyrum villosum T2DS·2V#4L translocation line in the background of Chinese Spring(CS) showed stable resistance to R. cerealis. Introgression of the T2DS·2V#4L chromosome into wheat cultivar Aikang 58 by backcrossing produced a marked increase in sharp eyespot resistance in NIL-T2DS·2V#4L in comparison with NILT2DS·2DL, and no detrimental effects of 2V#4L on agronomic traits were observed in the BC2F2, BC2F2:3,and BC2F2:4generations. Flow-sorted sequencing of 2V#4L yielded 384.3 Mb of assembled sequence, and8836 genes were predicted of which 6154 had orthologs in at least one of the 2AL, 2BL, and 2DL arms of CS, whereas 1549 genes were unique to 2V#4L. About 100,000 SNPs were detected in genes of 2V#4L and2DL in 10 sequenced bread wheat cultivars. A Kompetitive Allele Specific Polymerase chain reaction and30 conserved ortholog sequence markers were developed to trace the 2V#4L chromatin in wheat backgrounds. T2DS·2V#4L compensating translocation lines represent novel germplasm with sharp eyespot resistance and the markers will allow rapid detection in breeding programs.

关键词： Triticum aestivum Rhizoctonia cerealis Dasypyrum villosum Flow sorting KASP

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comparative analysis on grain quality and yield of different panicle weight indica-japonica hybrid rice(Oryza sativa L.) cultivars

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Journal of Integrative Agriculture 2020年第4期19卷 999-1009页

作者： BIAN Jin-long REN Gao-lei HAN Chao XU Fang-fu QIU Shi TANG Jia-hua ZHANG Hong-cheng WEI Hai-yan GAO Hui jiangsu Key Laboratory of Crop Genetics and Physiology/jiangsu co-Innovation center for Modern Production technology of Grain Crops/agricultural college Yangzhou UniversityYangzhou 225009P.R.China

Indica-japonica hybrid rice(Oryza sativa L.) cultivars showed high yield potential and poor tasting quality when compared with common japonica rice cultivars. Large panicle is a prominent factor of high yield for indica-japonica hybrid rice cultivars, and the panicle weight varies greatly among different indica-japonica hybrid rice cultivars. It is important to research on yield and grain quality of different panicle weight indica-japonica hybrid rice cultivars. In this study, two different panicle types indica-japonica hybrid cultivars were used to research on the relation of yield and grain quality. The yields of two heavy panicle weights indica-japonica hybrid cultivars were significantly higher than that of two medium panicle weight rice cultivars. The cooking and eating quality and starch properties of different panicle type cultivars were evaluated. Yongyou 6715(medium panicle) and Yongyou 1852(heavy panicle) got the relatively higher cooking and eating quality. Rice cultivars with medium panicle weight had more large starch granules and higher relative crystallinity than cultivars with heavy panicle weight. Transition temperature and retrogradation enthalpy(ΔHret) of medium panicle type cultivars were significantly higher than that of heavy panicle type cultivars. There was no significant difference in amylose content among different panicle type cultivars. Protein content of heavy panicle type cultivar was higher than that of medium panicle type cultivar, and protein content is the main factor affect cooking and eating quality in this study. The cultivar Yongyou 6715 got the highest taste value with the lowest protein content. Thus, it is suggested that the emphasis on improving rice cooking and eating quality of indica-japonica hybrid rice cultivars is how to reduce the protein content in rice grain. According to the results of this study, medium panicle type with high grain weight is the desired panicle type for high quality indica-japonica hybrid rice bre

关键词： yield eating and cooking quality starch amylose content protein content morphology thermal properties

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Effects of slow or controlled release fertilizer types and fertilization modes on yield and quality of rice

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Journal of Integrative Agriculture 2018年第10期17卷 2222-2234页

作者： WEI Hai-yan CHEN Zhi-feng XING Zhi-peng ZHOU Lei LIU Qiu-yuan ZHANG Zhen-zhen JIANG Yan HU Ya-jie ZHU Jin-yan CUI Pei-yuan DAI Qi-gen ZHANG Hong-cheng jiangsu Key Laboratory of Crop Genetics and Physiology/jiangsu co-Innovation center for Modem Production technology of Grain Crops/agricultural college Yangzhou University Yangzhou 225009 P.R.China

There is limited information about the influence of slow or controlled release fertilizer（S/CRF） on rice yield and quality. In this study, japonica rice cultivar Nanjing 9108 was used to study the effects of three different S/CRFs（polymer-coated urea（PCU）, sulfur-coated urea（SCU）, and urea formaldehyde（UF）） and two fertilization modes（both S/CRF and common urea（CU） as basal fertilizer, S/CRF as basal and CU as tillering fertilizer） on rice yield and quality. CU only was applied separately as control（CK）. Results showed that, rice grain yield, chalky kernel rate, chalky area, overall chalkiness, and the content of gliadin, glutenin, and protein, all showed the trends of UF〉PCU〉SCU within the same fertilization mode, and showed the trends of S/CRF as basal and CU as tillering fertilizer〉both S/CRF and CU as basal fertilizer within the same type of S/CRF. In contrast, the contents of amylose, amylopectin, and starch, as well as taste value, and peak and hot viscosity showed trends of SCU〉PCU〉UF, and the trends of both S/CRF and CU as basal fertilizer〉S/CRF as basal and CU as tillering fertilizer. Among S/CRF treatments and fertilization modes, taste values of cooked rice were positively correlated with amylose, amylopectin, and starch contents, as well as gel consistency, peak viscosity, hot viscosity, and cool viscosity, while negatively correlated with globulin, gliadin, glutenin, and protein contents. The types of S/CRF and fertilization modes are important for improving rice yield and quality. compared to CK, higher yield and similar quality of rice was achieved with UF as basal and CU as tillering fertilizer, and similar yield with improved appearance and eating and cooking quality of rice was achieved with either both UF and CU as basal fertilizer, or PCU as basal and CU as tillering fertilizer.

关键词： type of slow or controlled release fertilizer fertilization mode yield quality

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Morpho-physiological traits contributing to better yield performance of japonica/indica hybrids over indica hybrids under input-reduced practices

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Journal of Integrative Agriculture 2020年第11期19卷 2643-2655页

作者： WEI Huan-he MENG Tian-yao GE Jia-lin ZHANG Xu-bin LU Yu LI Xin-yue TAO Yuan DING Enhao CHEN Ying-long DAI Qi-gen jiangsu Key Laboratory of Crop Genetics and Physiology/jiangsu Key Laboratory of Crop Cultivation and Physiology/jiangsu co-Innovation center for Modern Production technology of Grain Crops/research Institute of Rice Industrial engineering technology Yangzhou UniversityYangzhou 225009P.R.China Institute of agricultural Science and technology Development Yangzhou University/Joint International Research Laboratory of Agriculture and Agri-Product SafetyMinistry of EducationYangzhou 225009P.R.China

It is widely reported that japonica/indica hybrids(JIH)have superior grain yield over other main varietal groups such as indica hybrids(IH)under sufficient resource *** date,little attention has been paid to yield performance of JIH under input-reduced practices,and whether JIH could have better grain yield performance over IH under input-reduced *** this study,three JIH varieties and three IH varieties were compared in grain yield and their related morpho-physiological traits under two cultivation modes,i.e.,conventional high-yielding method(CHYM)and double reductions in nitrogen rate and planting density(DRNP).Our results showed that JIH had 8.3 and 13.3%higher grain yield over IH under CHYM and DRNP,*** superior grain yield of JIH over IH under DRNP was mainly attributed to larger sink size and improved sink filling *** main morpho-physiological traits were concluded for better yield performance of JIH over IH under ***,JIH had the reduced unproductive tillers growth,indicated by a higher percentage of productive tillers and the percentage of effective leaf area index(LAI)to total LAI at heading ***,a synergistic increase in biomass accumulation and harvest index were achieved of JIH,supported by higher biomass accumulation and leaf area duration during the main growth periods,and improved non-structural carbohydrate(NSC)remobilization after ***,JIH had an improved canopy structure,showing as higher leaf area of upper three leaves and lower light extinction *** results suggested that improved morpho-physiological traits of JIH could lead to better grain yield performance over IH under input-reduced practices.

关键词： japonica/indica hybrid rice grain yield input-reduced practices

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Effects of temperature and solar radiation on yield of good eating-quality rice in the lower reaches of the Huai River Basin,China

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Journal of Integrative Agriculture 2021年第7期20卷 1762-1774页

作者： ZHOU Nian-bing ZHANG jun FANG Shu-liang WEI Hai-yan ZHANG Hong-cheng jiangsu Key Laboratory of Crop Genetics and Physiology/jiangsu co-Innovation center for Modern Production technology of Grain Crops Yangzhou UniversityYangzhou 225009P.R.China Huai’an agricultural technology Promotion center Huai’an 223001P.R.China

We studied the effects of temperature and solar radiation on rice yield with the aim of understanding the temperature and solar radiation requirements for high yield rice production in the lower reaches of the Huai River,*** experiments were conducted with two medium-maturing japonica rice(MMJR)varieties and four late-maturing japonica rice(LMJR)varieties in 2017 and *** were sown on May 10(T1),May 17(T2),May 24(T3),May 31(T4),June 7(T5),June 14(T6),and June 21(T7).The whole growth duration(WGD)of rice was shortened when sowing date was delayed,especially for the duration from sowing to heading(S–H).The effective accumulated temperature(EAT),mean daily temperature(Tmean),cumulative solar radiation(CSR),and mean daily solar radiation(Rmean)over the WGD decreased when sowing date was *** with T1,yields in T2,T3,T4,T5,T6,and T7 decreased by 0.12–0.35,0.45–0.89,0.74–1.56,1.41–2.24,2.16–2.90,and 2.69–3.64 t ha^(-1),*** was a significant positive correlation between rice yield and EAT in different growth *** was the main factor that affected the yield of good eating-quality rice in the lower reaches of the Huai *** found that a relatively high yield can be obtained when the optimal Tmean for medium-maturing japonica rice(MMJR)and late-maturing japonica rice(LMJR)was 25.8–27.0℃and 26.6–27.1℃in the stages from sowing to heading(S–H),and 20.3–23.3℃and 20.3–22.1℃in the stages from heading to maturity(H–M),*** optimal sowing dates for MMJR and LMJR in the lower reaches of the Huai River were May 15–31 and May 15–18,respectively.

关键词： rice yield temperature solar radiation growth stage

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Amino acids application enhances flowers insecticidal protein content in Bt cotton

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Journal of cotton research 2019年第1期2卷 38-43页

作者： TAMBEL Leila.I.M. ZHOU Mingyuan CHEN Yuan ZHANG Xiang CHEN Yuan CHEN Dehua jiangsu Key Laboratory of Crop Genetics and Physiology Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou University agricultural research cooperation Biotechnology and Biosafety Research Center

Background:Low insecticidal protein expression at reproductive organs affect insect resistance in Bt transgenic *** order to enhance flower insecticidal protein expression,the conventional cultivar Sikang1(S1)and the hybrid cultivar Sikang3(S3)were used as experimental materials;the applications of selected 5 types of amino acids and 21 types of amino acids were sprayed on the flowers in 2016 and 2017 cotton growing ***:The flower Bt protein contents increased significantly under the two amino acid treatments in both cultivars,the Bt protein concentration increased by 15.2 to 25.8%compared with the ***,no significant differences were detected between the two treatments of amino acid *** amino acid and soluble protein contents,enhanced GPT,GOT,protease,and peptidase activities were observed under the amino acid application at the flowering ***:These results suggest that exterior application of the amino acids treatments could bolster the flower insecticidal protein expression.

关键词： Bt cotton Flower Bt insecticidal protein Amino acid Nitrogen metabolism

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Fast detection of the early decay in oranges using visible-LED structuredillumination imaging combined with spiral phase transform and feature-based classification model

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International Journal of agricultural and Biological engineering 2024年第3期17卷 185-192,F0003页

作者： Zhonglei Cai Chanjun Sun Yizhi Zhang Ruiyao Shi Junyi Zhang Hailiang Zhang Jiangbo Li college of Mechanical and Electrical engineering Shihezi UniversityShihezi 832003XinjiangChina jiangsu Province and Education Ministry co-sponsored Synergistic Innovation center of Modern agricultural Equipment Jiangsu UniversityZhenjiang 212013JiangsuChina Intelligent Equipment research center Beijing Academy of Agriculture and Forestry SciencesBeijing100097China college of Electrical and Automation engineering East China Jiaotong UniversityNanchang 330013China National engineering research center for Information technology in Agriculture Beijing 100089China

The early decay of citrus can cause economic and serious food safety *** early decayed area has no obvious visual characteristics,making effective detection of this damage very difficult for the citrus *** study constructed a new detection system based on visible-light emitting diode(LED)structured-illumination imaging and proposed an effective methodology combined with a spiral phase transform(SPT)algorithm for the early detection of decayed *** sample obtained three phase-shifting pattern images with phase shifts of−2π/3,0,and 2π/3 at a spatial frequency of 0.25 cycles/*** strategies(i.e.,the conventional three-phase-shifting method,2-phase SPT,and 1-phase SPT)were used to demodulate the original patterned images to recover the direct component(DC)and amplitude component(AC)*** partial least squares discriminant analysis(PLS-DA)and least squares support vector machine(LS-SVM)classification models were established based on the texture features of DC,AC,and RT(*** ratio of AC to DC)***,the random frog(RF)algorithm was used to simplify the optimal full-featured ***,the LS-SVM model constructed using 7 texture features from the RT image obtained an average classification accuracy of 95.1%for all tested *** study indicates that the proposed structured-illumination imaging technique combined with 2-phase SPT and feature-based classification model can achieve the fast identification of early decayed oranges.

关键词： oranges early decay detection structured-illumination imaging spiral phase transform classification model

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SPSI:A Novel composite Index for Estimating Panicle Number in Winter Wheat before Heading from UAV Multispectral Imagery

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Plant Phenomics 2023年第3期5卷 642-656页

作者： Yapeng Wu Wenhui Wang Yangyang Gu Hengbiao Zheng Xia Yao Yan Zhu Weixing Cao Tao Cheng National engineering and technology center for Information Agriculture(NETCIA) MARA Key Laboratory for Crop System Analysis and Decision MakingMOE Engineering Research Center of Smart AgricultureJiangsu Key Laboratory for Information AgricultureNanjing Agricultural UniversityOne WeigangNanjingJiangsu 210095PR China Langfang Normal University 100 Aimin West RoadLangfangHebei 065000PR China

Rapid and accurate estimation of panicle number per unit ground area(PNPA)in winter wheat before heading is crucial to evaluate yield potential and regulate crop growth for increasing the final *** accuracies of existing methods were low for estimating PNPA with remotely sensed data acquired before heading since the spectral saturation and background effects were *** study proposed a spectral-textural PNPA sensitive index(SPSI)from unmanned aerial vehicle(UAV)multispectral imagery for reducing the spectral saturation and improving PNPA estimation in winter wheat before *** effect of background materials on PNPA estimated by textural indices(TIs)was examined,and the composite index SPSI was constructed by integrating the optimal spectral index(SI)and ***,the performance of SPSI was evaluated in comparison with other indices(SI and TIs).The results demonstrated that green-pixel TIs yielded better performances than all-pixel TIs apart from TI_([HOM]),TI_([ENT]),and TI_([SEM])among all indices from 8 types of textural ***,which was calculated by the formula DATT_([850,730,675])+NDTIcoR_([850,730]),exhibited the highest overall accuracies for any date in any dataset in comparison with DATT_([850,730,675]),TINDRE_([MEA]),and NDTIcoR_([850,730]).For the unified models assembling 2 experimental datasets,the RV^(2) values of SPSI increased by 0.11 to 0.23,and both RMSE and RRMSE decreased by 16.43%to 38.79%as compared to the suboptimal index on each *** findings indicated that the SPSI is valuable in reducing the spectral saturation and has great potential to better estimate PNPA using high-resolution satellite imagery.

关键词： heading Wheat indices

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Monitoring and identification of spoilage-related microorganisms in braised chicken with modified atmosphere packaging during refrigerated storage

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Food Science and Human Wellness 2023年第1期12卷 28-34页

作者： Yang Lei Yali Zhang Yiqun Cheng Jichao Huang Ming Huang Key Laboratory of Meat Processing and Quality control Ministry of EducationCollege of Food Science and TechnologyNanjing Agricultural UniversityNanjing 210095China National R&D center for Poultry Processing Jiangsu Research Center for Livestock and Poultry Products Processing Engineering TechnologyNanjing Huangjiaoshou Food Science and Technology Co.Ltd.Nanjing 211225China college of Environmental Science&engineering Institute of Functional FoodAnhui Normal UniversityWuhu 241000China college of engineering Nanjing Agricultural UniversityNanjing 210031China

This study mainly monitored the dominant bacterial populations and identified the spoilage-related microorganisms of braised chicken meat stored under different co_(2)-modified atmosphere packaging(MAP)during refrigerated storage using a culture-dependent method and 16S rDNA *** quality changes and shelf life of the meat were also *** growth rate of total viable count(TVC)in braised chicken was slower with an increase of co_(2) content in MAP,which also occurred in the remaining bacterial species monitored(lactic acid bacteria,Pseudomonas spp.,Brochothrix thermosphacta).The MAP exerted beneficial effects on the quality of braised chicken,as demonstrated by retarding the production of total volatile basic nitrogen(TVB-N)and delaying lipid oxidation(TBARS test).A total of 14 isolates were identified from braised chickens with different packaging at the end of storage,these included ***(6 isolates),***(2 isolates),Enterococcus faecalis(3 isolates),***(2 isolates),Staphylococcus equorum(1 isolate).

关键词： Braised chicken Modified atmosphere packaging(MAP) 16S rDNA identification Shelf-life

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The vital role of covS in the establishment of Streptococcus equi *** virulence

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Journal of Integrative Agriculture 2023年第2期22卷 568-584页

作者： XU Bin MA Zhe ZHOU Hong LIN Hui-xing FAN Hong-jie MOE Joint International research Laboratory of Animal Health and Food Safety College of Veterinary MedicineNanjing Agricultural UniversityNanjing 210095P.R.China Key Laboratory of Veterinary Biological engineering and technology of Ministry of Agriculture and Rural Affairs National Research Center of Veterinary Biologicals Engineering and TechnologyInstitute of Veterinary MedicineJiangsu Academy of Agricultural SciencesNanjing 210014P.R.China jiangsu co-innovation center for the Prevention and control of Important Animal Infectious Diseases and Zoonoses Yangzhou UniversityYangzhou 225009P.R.China

Streptococcus equi ***(SEZ)is an important zoonotic ***,a virulence-attenuated strain M35246 derived from natural variation of wild-type SEZ ATCC35246 was found.M35246 showed a deletion of 25contiguous genes as well as a loss-of-function mutation in ***,a 25-gene-deleted strain(ΔPI),a covS-mutant strain(Mcov S),and relevant complementary strains were constructed and investigated.M35246 and Mcov S were significantly less encapsulated and exhibited poorer anti-phagocytic capacity compared to wild-type *** was significantly more sensitive toβ-lactams,aminoglycosides,macrolides,and lincosamides than wild-type SEZ.M35246,McovS,andΔPI exhibited an increase in median lethal dose(LD_(50))in mice by 10~5,10~5,and 5 times when compared to wild-type SEZ,*** M35246 nor McovS were isolated from mice 48 h after being challenged with approximately 2000 times the LD_(50)of wild-type *** analysis showed that 668 significantly differentially expressed genes existed between McovS and wild-type *** virulence factor-encoding genes and anabolicrelated genes in McovS that were involved in anti-phagocytosis,capsule formation,pathogenicity,and antibiotic resistance were downregulated significantly relative to the wild-type *** study revealed that the covS plays a vital role in the establishment of SEZ virulence.

关键词： Streptococcus equi subsp.zooepidemicus covS natural variation virulence regulation

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