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Kalligrammatid Lacewings from the Upper Jurassic Daohugou Formation in Inner Mongolia, China

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Acta Geologica Sinica(English Edition) 2003年第2期77卷 141-147页

作者： ZHANG JunfengNanjing Institute of Geology and Palaeontology, Chinese academy of sciences, Nanjing, jiangsu 210008, E-mail: jfzhang@*** Chinese acad sci Nanjing Inst Geol & Palaeontol Nanjing 210008 Jiangsu Peoples R China

A new species, referable to a new genus, is erected, and named the Sinokalligramma jurassicum gen. et sp. nov. It is the second finding of kalligrammatids in the Daohugou Formation. The origin and migration of the family Kalligrammatidae are discussed. The geological age and stratigraphic correlation of the Daohugou and Karabastau Formations are briefly reviewed and reassessed.

关键词： kalligrammatid lacewings new taxa age origin and migration Jurassic Inner Mongolia China

Expression of tachyplesin gene in yeast Pichia pastoris

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Chinese science Bulletin 1998年第23期43卷 1986-1991页

作者： Chunyi Zhang Jun Zhao Changjian Wu Yunliu Fan Chinese acad agr sci Biotechnol Res Ctr Beijing 100081 Peoples R China

Tachyplesin gene was designed and chemically synthesized with codon usage bias. The stop codon TAG and some restriction sites convenient for further cloning were added to this gene. The synthesized gene cloned into yeast expression vector pPIC9 (with α_secretion signal) was transformed into host strain GS115 by electroporation. The tachyplesin expressed from recombinants Y PIC27 and Y PIC42 showed an inhibition activity against the germination of the spores of \%Magnaporthe grisea\%. Southern blot performed with chromosome genome of the two recombinants indicated a single copy of the expression cassette was integrated at the chromosomal AOX 1 locus by which the genomic AOX 1 gene was functionally disrupted and Northern blot showed the presence of transcripts of the tachyplesin gene.

关键词： tachyplesin gene expression \%Pichia pastoris.\%

Study on The Relationship of Laser Power Density and Localization Precision in PALM Imaging

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生物化学与生物物理进展 2017年第6期44卷 534-536页

作者： Fan Chun-Yan Li Yuan-Yuan Gu Lu-Sheng Li Wei-Xing Xu Xiao-Jun Zhang Shu-Wen Wang Lei Chen Yan Xue Yan-Hong Ji Wei Chinese acad sci Inst Biophys Natl Lab Biomacromol Beijing 100101 Peoples R China Univ Chinese acad sci Beijing 100049 Peoples R China Chinese acad sci Inst Biophys Ctr Biol Instrument Dev Beijing 100101 Peoples R China Foshan Univ Foshan 528000 Peoples R China jiangsu acad sci & Technol Dev Nanjing 210042 Jiangsu Peoples R China

近十年来，基于单分子定位的PALM成像技术快速发展，将显微镜的分辨率提高到了2~25nm. 本文发现PALM成像过程中采用的激发光强度与成像的定位精度之间有密切的联系. 我们分别选择了PALM成像使用的光激活荧光蛋白、光转换荧光蛋白和光开... 详细信息

近十年来，基于单分子定位的PALM成像技术快速发展，将显微镜的分辨率提高到了2~25nm. 本文发现PALM成像过程中采用的激发光强度与成像的定位精度之间有密切的联系. 我们分别选择了PALM成像使用的光激活荧光蛋白、光转换荧光蛋白和光开关荧光蛋白中最常用的荧光蛋白进行验证. 实验发现伴随激光强度的增加，大部分荧光蛋白的光子数先升高然后趋于饱和，背景噪声几乎线性升高. 进一步分析发现荧光蛋白的定位误差随着激光强度增强先降低后升高，因此选用合适的激光强度在PALM成像实验中至关重要. 如何提高PALM成像的分辨率一直是科学家研究的热点，本研究内容可以指导研究人员在PALM成像中选用合适的激发光强度，从而得到高分辨率的图像.

关键词：英语阅读理解生物化学生物物理

The Tartary Buckwheat Genome Provides Insights into Rutin Biosynthesis and Abiotic Stress Tolerance

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Molecular Plant 2017年第9期10卷 1224-1237页

作者： Zhang, Lijun Li, Xiuxiu Ma, Bin Gao, Qiang Du, Huilong Han, Yuanhuai Li, Yan Cao, Yinghao Qi, Ming Zhu, Yaxin Lu, Hongwei Ma, Mingchuan Liu, Longlong Zhou, Jianping Nan, Chenghu Qin, Yongjun Wang, Jun Cui, Lin Liu, Huimin Liang, Chengzhi Qiao, Zhijun Shanxi acad agr sci Inst Crop Germplasm Resources Res Taiyuan 030031 Shanxi Peoples R China Minist agr Key Lab Crop Gene Resources & Germplasm Enhanceme Taiyuan 030031 Shanxi Peoples R China Shanxi Key Lab Genet Resources & Genet Improvemen Taiyuan 030031 Shanxi Peoples R China Chinese acad sci Inst Genet & Dev Biol State Key Lab Plant Genom Beijing 100101 Peoples R China Univ Chinese acad sci Coll Life Sci Beijing 100049 Peoples R China Shanxi agr Univ Coll Agron Taiyuan 030801 Shanxi Peoples R China Chinese acad sci Inst Microbiol State Key Lab Microbial Resources Beijing Peoples R China South China agr Univ Coll Marine Sci Guangzhou 510642 Guangdong Peoples R China

Tartary buckwheat （Fagopyrum tataricum） is an important pseudocereal crop that is strongly adapted to growth in adverse environments. Its gluten-free grain contains complete proteins with a well-balanced composition of essential amino acids and is a rich source of beneficial phytochemicals that provide significant health benefits. Here, we report a high-quality, chromosome-scale Tartary buckwheat genome sequence of- 489.3 Mb that is assembled by combining whole-genome shotgun sequencing of both Illumina short reads and single-molecule real-time long reads, sequence tags of a large DNA insert fosmid library, Hi-C sequencing data, and BioNano genome maps. We annotated 33 366 high-confidence protein-coding genes based on expression evidence. Comparisons of the intra-genome with the sugar beet genome revealed an independent whole-genome duplication that occurred in the buckwheat lineage after they diverged from the common ancestor, which was not shared with rosids or asterids. The reference genome facilitated the identification of many new genes predicted to be involved in rutin biosynthesis and regulation, aluminum stress resistance, and in drought and cold stress responses. Our data suggest that Tartary buckwheat＇s ability to tolerate high levels of abiotic stress is attributed to the expansion of several gene families involved in signal transduction, gene regulation, and membrane transport. The availability of these genomic resources will facilitate the discovery of agronomically and nutritionally important genes and genetic improvement of Tartary buckwheat.

关键词： tartary buckwheat de novo genome assembly rutin biosynthesis aluminum resistance abiotic stress,whole genome duplication

Expression of rabbit defensin NP-1 gene in transgenic tobacco plants and its activity against bacterial wilt

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Chinese science Bulletin 1998年第18期43卷 1544-1550页

作者： Rongzhao Fu Yufa Peng Guangcheng Cao Jiangsheng Ma Caixia Chen Liming Zhang Wenbin Li Yongru Sun Chinese acad sci Genet Inst Beijing 100101 Peoples R China Chinese acad agr sci Inst Plant Protect Beijing 100094 Peoples R China

A cDNAderived 200 bp fragment encoding a rabbit defensin NP1 mature peptide was ligated into the GUSless pBI121 to yield pBIC35NP1. Tobacco leaf discs were genetically transformed with an agrobacterium tumefaciens strain harbouring pBIC35NP1. Northern blot analysis showed that the defensin NP1 expression cassette was normally transcribed in transgenic plants. Resistance tests demonstrated that expression of native defensin NP1 can confer partial resistance to the bacterial wilt pathogen, Ralstonia solanacearum.

关键词： bacterial disease_resistance rabbit defensin tobacco transgenic plant.

Gene cloning and function analysis of ABP9 protein which specifically binds to ABRE2 motif of maize Cat1 gene

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Chinese science Bulletin 2002年第22期47卷 1871-1875页

作者： Lei Wang Jun Zhao Yunliu Fan Chinese acad agr sci Biotechnol Res Inst Beijing 100081 Peoples R China

A cDNA library was constructed using mRNA extracted from 17 days post-pollination (dpp) maize embryos and was screened by employing a yeast one-hybrid system for proteins specifically interacting with ABRE2 motif of maize Call gene. Three truncated overlapping positive clones designated ABP9 were obtained and the full-length cDNA was isolated by 5’ RACE. Searching the database revealed that ABP9 protein belongs to a bZIP-type transcription factor family. ABP9 protein speciflcally binds to ABRE2 motif and activates the expression of downstream reporter gene in yeast cells. Our results strongly suggest that the ABP9 protein functions as a transcription activator.

关键词： maize ABRE ABP transcription factor function analysis.

Evaluation of transgenic tobacco expressing two insecticidal genes to delay resistance development of Helicoverpa armigera

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Chinese science Bulletin 1999年第20期44卷 1871-1874页

作者： Jianzhou Zhao Yunliu Fan Xianlin Fan Xiping Shi Meiguang Lu Chinese acad agr sci Inst Plant Protect State Key Lab Pests & Dis Beijing 100094 Peoples R China Chinese acad agr sci Biotechnol Res Ctr Beijing 100081 Peoples R China

The resistance ratio of Helicoverpa armigera to Cry1Ac insecticidal protein from Bacillus thuringiensis (Bt) is 13.1- and 3.02-fold after 18 generations of selection by transgenic tobacco expressing Bt or two (Bt and CpTI) insecticidal protein genes, in which the average corrected mortality for each selection treatments is about 60% . The mortality of selected population by transgenic Bt gene tobacco is significantly lower than the control strain when fed

关键词： transgenic tobacco Bt CpTI Helicoverpa armigera resistance.

A Sequencing-Based Linkage Map of Cucumber

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Molecular Plant 2015年第6期8卷 961-963页

作者： Zhou, Qian Miao, Han Li, Shuai Zhang, Shengping Wang, Ye Weng, Yiqun Zhang, Zhonghua Huang, Sanwen Gu, Xingfang Chinese acad agr sci Key Lab Biol & Genet Improvement Hort Crops Inst Vegetables & Flowers Minist AgrSino Dutch Joint Lab Hort Gen Beijing 10081 Peoples R China Chinese acad agr sci Agr Genom Inst Shenzhen Shenzhen 518120 Peoples R China Univ Wisconsin Vegetable Crops Res Unit Dept Hort USDAARS Madison WI 53706 USA

Cucumber （Cucumis sativus L., 2n = 2x = 14） is an economically important vegetable worldwide and has served as a model sys- tem for plant vascular biology and sex determination studies. We reported the draft genome assembly of the North China type cucumber line 9930 in 2009 （Huang et al., 2009a） and constructed a single-base resolution map of cucumber genomic variation by resequencing of the genomes of 115 core germplasm lines （Qi et al., 2013）, which enabled genome-wide association studies of important traits in cucumber （Shang et al., 2014）.

关键词： Sequencing-Based Linkage Map

Evolution of Strigolactone Perception by Seeds of Parasitic Plants： Reinventing the Wheel

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Molecular Plant 2016年第4期9卷 493-495页

作者： Xiong, Guosheng Li, Jiayang Smith, Steven M. Chinese acad agr sci Agr Genom Inst Shenzhen Shenzhen 518124 Peoples R China Chinese acad sci State Key Lab Plant Genom Inst Genet & Dev Biol Beijing 100101 Peoples R China Chinese acad sci Natl Ctr Plant Gene Res Beijing Inst Genet & Dev Biol Beijing 100101 Peoples R China Univ Tasmania Sch Biol Sci Hobart Tas 7001 Australia

A BEWITCHING PROCESS The root-parasitic plants of the witchweed family （Orobanchan- ceae） produce seeds that are adapted to germinate in response to strigolactones （SLs） exuded from the roots of their host plants. The resulting colonization of crops such as maize and sorghum by these parasites can cause massive losses in production and to the livelihoods of farmers （Westwood et al., 2010）.

关键词： root-parasitic plants witchweed