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Gastrointestinal digestion fates of lipids based on pH-stat and Caco-2 cells models: Superior bioavailability of triglyceride monomer derived from silkworm pupae oil

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FOOD BIOsciENCE 2023年第3期53卷 1900-1908页

作者： Yan, Cheng-Hai Xun, Xiao-Meng Herman, Richard A. Zhan, Yu-Fan Gong, Lu-Chan Wu, Fu-An Wang, Jun jiangsu Univ sci & Technol Sch Biotechnol Jiangsu Key Lab Sericultural Biol & Biotechnol Zhenjiang 212100 Jiangsu Peoples R China Chinese acad agr sci Sericultural Res Inst Key Lab Silkworm & Mulberry Genet Improvement Minist Agr & Rural Affairs Zhenjiang 212100 Jiangsu Peoples R China

Silkworm pupae oil (SPO) was regarded as a good alternative to alpha-linolenic acid supplements, which contain a variety of triacylglyceride with high nutritional value. To investigate the connections between triacylglyceride composition and lipid gastrointestinal digestive absorption profiles, isolation and bioavailability of tri-acylglyceride monomers from silkworm pupae oil was analyzed. In this study, two triacylglyceride monomers rich in alpha-linolenic acid were separated by preparative liquid chromatography, namely LnLnLn (1,2,3-alpha-linolenic acid triacylglyceride) and LnLnL (1,2-alpha-linolenic acid, 3-linoleic acid triacylglyceride). The in vitro digestion in the pH-stat model results showed the digestive rate of LnLnLn was 0.4101 +/- 0.0089 s-1, which was significantly higher than that of SPO (0.2494 +/- 0.0108 s-1) (p < 0.05). The in vitro absorption in the Caco-2 cells model results showed the absorptivity of LnLnLn and LnLnL were 6.40 +/- 0.19% and 6.06 +/- 0.54%, which were significantly higher than SPO (5.43 +/- 0.51%) (p < 0.05). In addition, the mRNA expression levels of lipid metabolism-related proteins provide evidence for the absorption superiority of LnLnLn and LnLnL. The present work provides novel structural lipids for rapid and efficient supplementation of alpha-linolenic acid.

关键词： Silkworm pupae oil Separation Triacylglyceride monomer Bioavailability pH-stat Caco-2 cells In vitro

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Risk assessment of Bacillus cereus in lettuce grown in open-field and greenhouse farming systems in China: A comparative study

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FOOD QUALITY AND SAFETY 2024年第8期8卷 268-277页

作者： Qu, Yang Yu, Shenghao Gu, Haotian Wang, Wen Wang, Wenbo He, Zhaoying Shan, Hong Gao, Min Zhao, Xin Wei, Chao Tao, Tingting Zhao, Guanghua Zhou, Changyan Suo, Yujuan Shanghai acad agr sci Inst Agrofood Stand & Testing Technol Lab Qual & Safety Risk Assessment Agroprod Minist Agr & Rural Affairs Shanghai Peoples R China Shanghai Municipal Adm Market Regulat Informat Applicat Res Ctr Shanghai Peoples R China Shanghai acad agr sci Ecoenvironm Protect Res Inst Shanghai Peoples R China Zhejiang acad agr sci Inst Agroprod Safety & Nutr MOA Lab Qual & Safety Risk Assessment Agroprod Han Hangzhou Peoples R China Shandong acad agr sci Inst Agr Stand & Testing Technol Agriprod Lab Qual & Safety Risk Assessment Agroprod Minist Agr & Rural Affairs Jinan Peoples R China Beijing acad agr & Forestry sci Inst Qual Stand & Testing Technol Beijing Peoples R China Heilongjiang acad agr sci Qual & Safety Inst Agr Prod Harbin Peoples R China Chinese acad agr sci Inst Food Sci & Technol Beijing Peoples R China Tianjin acad agr sci Inst Germplasm Resources & Biotechnol Tianjin Peoples R China Sichuan acad agr sci Inst Qual Stand & Testing Technol Agroprod Lab Qual & Safety Risk Assessment Agroprod Minist Agr & Rural Affairs Chengdu Peoples R China jiangsu acad agr sci Inst Food Safety & Nutr Key Lab Control Technol & Stand Agroprod Safety & Minist Sci & Technol Nanjing Peoples R China Henan acad & agr sci Res Inst Agr Qual Stand & Testing Tech Zhengzhou Peoples R China

Objectives: Bacillus cereus isolates from vegetables can cause diarrheal syndrome food poisoning. Lettuce, the most highly consumed ready-to-eat vegetable, poses a significant risk of B. cereus exposure. Hence, B. cereus monitoring and evaluation during cultivation are essential for preventing foodborne illnesses caused by this food-pathogen combination. However, the impact of different cultivation practices on B. cereus contamination in lettuce currently remains unclear. Furthermore, a validity indicator for the experimental models cannot be effectively validated under field conditions. Materials and Methods: Herein, we assessed the risk of B. cereus exposure using lettuce grown under two different cultivation farming systems in China (open-field versus greenhouse) using a quantitative microbial risk assessment model. Tukey's mean comparison method and kernel density estimation frequency distribution statistics were used for model validation using environmental samples and lettuce. Results: The mean concentration of B. cereus in open-field-grown lettuce was significantly higher than that in greenhouse-grown lettuce (P0.05), verifying the accuracy of the risk model. Conclusion: Soil solarization, complete manure treatment, usage of mulch films, and irrigation water quality should be considered in agricultural practices to control B. cereus concentrations in lettuce. Overall, we found that B. cereus was more abundant in open-field-grown lettuce, and we provide a risk evaluation framework that can guide farmers and policy-makers to control B. cereus contamination in farm-to-fork lettuce chains in China.

关键词： Diarrheal syndrome food poisoning ready-to-eat vegetable greenhouse microbial risk assessment

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Cloning and characterization of a C genome-specific repetitive sequence in Aegilops caudata L.

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Chinese science Bulletin 1999年第14期44卷 1301-1306页

作者： Xiuying Kong Ronghua Zhou Yuchen Dong Jizeng Jia Chinese acad agr sci Minist Agr Key Lab Crop Germplasm & Biotechnol Beijing 100081 Peoples R China

pAeca212 is 204 bp in length, and the G + C content is 51%. It disperses on all seven chromosome pairs of Aegilops caudata except centromeres and secondary *** with the 316893 DNA sequences registered in Genbank/EMBL/DDJB/PDB, pAeca212 is a new C-genome specific repetitive sequence. The results of genomic specificity analysis of pAeca212 show that there are no hybridization signals detected in all donor Poaceae plants except in rye. pAeca212 is a very useful molecular marker in the study of the origin of Triticeace and the detection of C chromatin in wheat background.

关键词： Aegilops caudata genome specific repetitive sequence.

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Kalligrammatid Lacewings from the Upper Jurassic Daohugou Formation in Inner Mongolia, China

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Acta Geologica Sinica(English Edition) 2003年第2期77卷 141-147页

作者： ZHANG JunfengNanjing Institute of Geology and Palaeontology, Chinese academy of sciences, Nanjing, jiangsu 210008, E-mail: jfzhang@*** Chinese acad sci Nanjing Inst Geol & Palaeontol Nanjing 210008 Jiangsu Peoples R China

A new species, referable to a new genus, is erected, and named the Sinokalligramma jurassicum gen. et sp. nov. It is the second finding of kalligrammatids in the Daohugou Formation. The origin and migration of the family Kalligrammatidae are discussed. The geological age and stratigraphic correlation of the Daohugou and Karabastau Formations are briefly reviewed and reassessed.

关键词： kalligrammatid lacewings new taxa age origin and migration Jurassic Inner Mongolia China

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Expression of tachyplesin gene in yeast Pichia pastoris

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Chinese science Bulletin 1998年第23期43卷 1986-1991页

作者： Chunyi Zhang Jun Zhao Changjian Wu Yunliu Fan Chinese acad agr sci Biotechnol Res Ctr Beijing 100081 Peoples R China

Tachyplesin gene was designed and chemically synthesized with codon usage bias. The stop codon TAG and some restriction sites convenient for further cloning were added to this gene. The synthesized gene cloned into yeast expression vector pPIC9 (with α_secretion signal) was transformed into host strain GS115 by electroporation. The tachyplesin expressed from recombinants Y PIC27 and Y PIC42 showed an inhibition activity against the germination of the spores of \%Magnaporthe grisea\%. Southern blot performed with chromosome genome of the two recombinants indicated a single copy of the expression cassette was integrated at the chromosomal AOX 1 locus by which the genomic AOX 1 gene was functionally disrupted and Northern blot showed the presence of transcripts of the tachyplesin gene.

关键词： tachyplesin gene expression \%Pichia pastoris.\%

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Expression of rabbit defensin NP-1 gene in transgenic tobacco plants and its activity against bacterial wilt

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Chinese science Bulletin 1998年第18期43卷 1544-1550页

作者： Rongzhao Fu Yufa Peng Guangcheng Cao Jiangsheng Ma Caixia Chen Liming Zhang Wenbin Li Yongru Sun Chinese acad sci Genet Inst Beijing 100101 Peoples R China Chinese acad agr sci Inst Plant Protect Beijing 100094 Peoples R China

A cDNAderived 200 bp fragment encoding a rabbit defensin NP1 mature peptide was ligated into the GUSless pBI121 to yield pBIC35NP1. Tobacco leaf discs were genetically transformed with an agrobacterium tumefaciens strain harbouring pBIC35NP1. Northern blot analysis showed that the defensin NP1 expression cassette was normally transcribed in transgenic plants. Resistance tests demonstrated that expression of native defensin NP1 can confer partial resistance to the bacterial wilt pathogen, Ralstonia solanacearum.

关键词： bacterial disease_resistance rabbit defensin tobacco transgenic plant.

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Gene cloning and function analysis of ABP9 protein which specifically binds to ABRE2 motif of maize Cat1 gene

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Chinese science Bulletin 2002年第22期47卷 1871-1875页

作者： Lei Wang Jun Zhao Yunliu Fan Chinese acad agr sci Biotechnol Res Inst Beijing 100081 Peoples R China

A cDNA library was constructed using mRNA extracted from 17 days post-pollination (dpp) maize embryos and was screened by employing a yeast one-hybrid system for proteins specifically interacting with ABRE2 motif of maize Call gene. Three truncated overlapping positive clones designated ABP9 were obtained and the full-length cDNA was isolated by 5’ RACE. Searching the database revealed that ABP9 protein belongs to a bZIP-type transcription factor family. ABP9 protein speciflcally binds to ABRE2 motif and activates the expression of downstream reporter gene in yeast cells. Our results strongly suggest that the ABP9 protein functions as a transcription activator.

关键词： maize ABRE ABP transcription factor function analysis.

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Evaluation of transgenic tobacco expressing two insecticidal genes to delay resistance development of Helicoverpa armigera

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Chinese science Bulletin 1999年第20期44卷 1871-1874页

作者： Jianzhou Zhao Yunliu Fan Xianlin Fan Xiping Shi Meiguang Lu Chinese acad agr sci Inst Plant Protect State Key Lab Pests & Dis Beijing 100094 Peoples R China Chinese acad agr sci Biotechnol Res Ctr Beijing 100081 Peoples R China

The resistance ratio of Helicoverpa armigera to Cry1Ac insecticidal protein from Bacillus thuringiensis (Bt) is 13.1- and 3.02-fold after 18 generations of selection by transgenic tobacco expressing Bt or two (Bt and CpTI) insecticidal protein genes, in which the average corrected mortality for each selection treatments is about 60% . The mortality of selected population by transgenic Bt gene tobacco is significantly lower than the control strain when fed

关键词： transgenic tobacco Bt CpTI Helicoverpa armigera resistance.

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Chromosomal location of yellow rust resistance gene(s) in Triticum aestivum-Lophopyrum elongatum substitution lines

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Chinese science Bulletin 1999年第4期44卷 344-348页

作者： Jianxin Ma Ronghua Zhou Yuchen Dong Jizeng Jia Chinese acad agr sci Inst Crop Germplasm Resources Beijing 100081 Peoples R China

A set of T. aestivum-L. elongatum substitution lines were studied on yellow rust resistance at seedling stage, inheritance of the resistance and esterase-5 (Est-5) analysis. The results demonstrated that L. elongatum carried a new yellow rust resistance gene(s), which was dominant and located on chromosome 3E of L. elongatum. The biochemical locus encoding Est-5 was also located on chromosome 3E, and was tentatively named Est-E5, which was co-segregant with the Yr gene(s) in wheat background. In addition, the transmission frequencies of chromosome 3E in 3A/3E and 3D/3E hybrids selfing were significantly higher than that of chromosome 3E in 3B/3E hybrid, which was probably due to the difference on genetic relationships among A, B, D and E genomes.

关键词： Triticum aestivum-Lophopyrum elongatum substitution lines yellow rust resistance gene location biochemical marker.

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Genetic study on the behavior of honeybees influenced by polyandry system

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Chinese science Bulletin 1999年第20期44卷 1895-1898页

作者： Yan Ouyang Liming Wu Feng Jin Wei Shi Pingping Tan Qinhua Bai Xianshu Liu Chinese acad sci Inst Genet Beijing 100101 Peoples R China Chinese acad agr sci Inst Apicultural Res Beijing 100093 Peoples R China

Within a honeybee population, due to polyandry, there are super-sister and half-sister relations, thus many sub-families exist. For the Chinese honeybee (Apis cerana cerana F.) a phylogenetic dendrogram has been constructed in which 4 sub-families are clustered based on DNA fingerprint patterns. And it has been observed that each kind of workers is distributed to several different sub-families.

关键词： Apis cerana cerana F. DNA fingerprints polyandry behavior.

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