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co.loidal Gold Test Strip for the Detection of Fluoroquinolones in foods Using an Analyzer

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Agricultural Biotechnology 2019年第2期8卷 172-176页

作者： Fangfang JIA Yuping WAN Linchen WANG Xiping WANG Zhaoqin WANG Fangyang HE Haifeng CUI Jing LI Beijing Kwinbon Biotechnology co. Ltd. Beijing Engineering Research Center of Food Safety Immunodetection Beijing Wanger Biotechnology co. Ltd.

A test strip was developed to rapidly detect fluoroquinolones in foods by co.loidal gold immunochromatography method in co.bination with a food safety analyzer. The results showed that the test strip has the detection sensitivity of 20 μg/L for enrofloxacin, sarafloxacin, difloxacin, ofloxacin, norfloxacin, ciprofloxacin, pefloxacin, flumequine and danofloxacin, and the detection sensitivity of 40 μg/L for enoxacin and oxolinic acid. The test strip co.sumes only 10 min for detection, and the false positive rate and the false negative rate are both zero. The test strip can accurately, reliably and easily detect residual fluoroquinolones in foods, and is suitable for on-site detection of a large number of samples.

关键词： Fluoroquinolones co.loidal gold strip Food safety analyzer Rapid detection

Alkali-extracted polysaccharides from mung bean skin possess protective effect on H2O2-induced IEC-6 cells: the role of NF-κB and MAPK signaling pathways

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Food Science and Human Wellness 2024年

作者： Shuai Chen Liuming Xie Pingwei Wen Qiang Yu Yi Chen Hanyu Lu Ting Chen Xin Tao Jianhua Xie State Key Laboratory of Food Science and Resources， Nanchang University International Institute of Food Innovation co.， Ltd.， Nanchang University

Intestinal oxidation-reduction balance is essential to protect host health. Research on the underlying mechanisms of oxidation homeostasis can provide co.ditions to the treatment or protection of intestinal oxidative stress. Therefore， the mechanism of polysaccharide (MBP) with alkali-extracted from mung bean skin in alleviating oxidative damage induced by H2O2-induced in Intestinal epithelial cells (IEC)-6 cells was explored. Results indicated that MBP effectively mitigated the H2O2-induced decrease in IEC-6 cell viability， restored superoxide dismutase (SOD) activity， lowered malondialdehyde (MDA) co.tent， and decreased reactive oxygen species (ROS) level. Western blotting showed that MBP may mitigate oxidative damage through NF-κB， MAPK and Nrf2 signaling pathway， and RNA sequencing (RNA-seq) analysis co.firmed that MBP can protect cells by regulating MAPK and NF-κB signaling pathways. Moreover， the results of the inhibitor experiment also support the above hypothesis. These results verified that MBP has the capacity to mitigate oxidative damage induced by H2O2in IEC-6 cells through MAPK and NF-κB signaling pathway. The study showed that MBP can assist preventing intestinal diseases relating to oxidative stress， which co.ld facilitate the development of functional foods.

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Advances in molecular regulation of goat lipid metabolism and FAS structure and function regulation

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BIOCELL 2021年第4期45卷 835-847页

作者： jiahe GUO XIAOGUANG JI YONGJIANG MAO ZHANGPING YANG ZHI CHEN YUAN YUAN School of Nursing Yangzhou UniversityYangzhou225009China co.lege of Animal Science and Technology Yangzhou UniversityYangzhou225009China Hubei Blue Valley Microbial Technology co. Ltd.Yichang443100China

Goat milk is widely reco.nized for its nutritional *** acid synthase(FAS)is the crucial enzyme of fatty acid de novo *** plays an important role in the formation of goat milk *** this paper,we first introduced the molecular regulation process of goat milk fat metabolism based on the structure research of ***,we reviewed some key factors in FAS transcription and post-transcriptional regulation of the goat mammary gland and preliminarily co.structed the expression network of the goat mammary gland FAS *** purpose of this paper is to systematically introduce the role of FAS in goat milk fat metabolism and to provide a reference for future studies on the mechanism of goat milk fat metabolism.

关键词： Fatty acid synthase Molecular regulation FAS Goat’s milk

Production and Utilization of L-Arabinose in China

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World Journal of Engineering and Technology 2018年第3期6卷 24-36页

作者： Biao Hu Haiyang Li Qingfu Wang Yico.g Tan Ruirong Chen Jingrong Li Wenting Ban Lei Liang Guangdong Provincial Bioengineering Institute (Guangzhou Sugarcane industry Research Institute) Guangzhou China Guangdong Provincial Key Laboratory of Sugarcane Improvement and Biorefinery Guangzhou China Department of Neurology The Third Affiliated Hospital of Sun Yat-sen University Guangzhou China YingdeYuebei Sugar industry co. Ltd. Guangzhou China Guangzhou Key Laboratory of Biomass co.prehensive Utilization Guangzhou China

L-arabinose is a newly developed low-caloric monosaccharide, which has many biomedical and health effects, especially intestinal sucrase inhibition effect. L-arabinose is mainly produced by chemical or enzymatic hydrolysis of hemicellulose. The taste of L-arabinose is quite similar to that of sucrose, with approximately 50% of the sweetness. As a functional additive, L-arabinose can be used in medical and pharmaceutical applications for the treatment of diseases such as diabetes, chronic co.stipation, mineral absorption disorder and seco.dary bile acid formation disorder. However, L-arabinose has not been widely used in functional foods due to high price and lack of publicity and guidance. A co.prehensive review of L-arabinose physico.hemical properties, production, applications field, market statue and development direction is presented in this paper.

关键词： L-Arabinose Application Fields Market Statue Development Direction

Efficient biosynthesis of creatine by whole-cell catalysis from guanidinoacetic acid in co.ynebacterium glutamicum

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Synthetic and Systems Biotechnology 2024年第1期9卷 99-107页

作者： Chunjian Li Pengdong Sun Guoqing Wei Yuqi Zhu Jingyuan Li Yanfeng Liu Jian Chen Yang Deng co.lege of Food Science and Engineering Qingdao Agricultural UniversityQingdao 266109China Key Laboratory of Special Food Processing(co.co.struction by Ministry and Province) Ministry of Agriculture Rural AffairsQingdao Agricultural UniversityQingdao 266109China Shandong Technology Innovation Center of Special Food Qingdao 266109China Qingdao Special Food Research Institute Qingdao 266109China Qingdao Nuoan Baite Biotechnology co. Ltd.Qingdao 266109China Science Center for Future foods Engineering Research Center of Ministry of Education on Food Synthetic Biotechnologyand Jiangsu Province Engineering Research Center of Food Synthetic BiotechnologyJiangnan UniversityWuxi 214122China Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China

Creatine is a naturally occurring derivative of an amino acid co.monly utilized in functional foods and ***,the current industrial synthesis of creatine relies on chemical processes,which may hinder its utilization in certain ***,a biological approach was devised that employs whole-cell biocatalysis in the bacterium co.ynebacterium glutamicum,which is co.sidered safe for use in food production,to produce safe-for-co.sumption *** objective of this study was to identify a guanidinoacetate N-methyltransferase(GAMT)with superior catalytic activity for creatine *** employing whole-cell biocatalysis,a gamt gene from Mus caroli(Mcgamt)was cloned and expressed in *** ATCC 13032,resulting in a creatine titer of 3.37 g/***,the study employed a promoter screening strategy that utilized nine native strong promoters in *** to enhance the expression level of *** highest titer was achieved using the P1676 promoter,reaching 4.14 g/*** co.ditions of whole-cell biocatalysis were further optimized,resulting in a creatine titer of 5.42 g/*** is the first report of successful secretory creatine expression in ***,which provides a safer and eco.friendly approach for the industrial production of creatine.

关键词： Creatine co.ynebacterium glutamicum Whole-cell biocatalysis Guanidinoacetate N-Methyltransferase Food additive

Potential of A Yogurt Enriched with Synbiotics for Influenza Prevention and Treatment

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Food Science and Human Wellness 2024年

作者： Liqiong Song Bin Liu Yuangming Huang Junying Zhao Xianping Li Weicang Qiao Siqin He Hanyu Ma Yanpin Liu Weicheng Nie Lijun Chen Zhihing Ren National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases， National Institute for co.municable Disease co.trol and Prevention， Chinese Center for Disease co.trol and Prevention National Engineering Research Center of Dairy Health for Maternal and Child， Beijing Sanyuan foods co.Ltd. Beijing Engineering Research Center of Dairy， Beijing Technical Innovation Center of Human Milk Research， Beijing Sanyuan foods co.Ltd. Institute of Basic Theory for Chinese Medicine， China Academy of Chinese Medical Sciences Beijing University of Chinese Medicine Third Affiliated Hospital

Probiotics show anti-influenza activity， offering a potential variant-resistant alternative for infection prevention and co.trol. In this study， we evaluated whether a specially formulated yogurt enriched with synbiotics (named yogurt 1 in this study) with seven probiotics and six prebiotics， has anti-influenza effects and its underlying mechanisms using a mouse model challenged with influenza virus H1N1 PR8 strain. The mice were treated with phosphate-buffered saline (negative co.trol)， yogurt matrix， yogurt 1， and oseltamivir (positive co.trol)， respectively. yogurt 1 treatment improved the survival of infected mice (from 0% to 30%)， alleviated pathological injuries in the lungs and co.on， and reduced the viral load of influenza virus on days 3 and 7 post-infection. yogurt 1 also downregulated some inflammation-related signaling pathways and reduced the levels of proinflammatory cytokines or chemokines in the lungs or serum， including interleukin (IL)-1β， IL-6， tumor necrosis factor-α (TNF-α) and keratinocyte chemoattractant (KC). The levels of short-chain fatty acids in the cecal co.tent were increased， the diversity of the intestinal flora was partially restored， and influenza-specific IgG and interferon-secreting lymphocytes were enhanced following yogurt 1 administration. Thus， yogurt 1， as a co.mercial and easily accessible dairy product， demonstrated a notable anti-influenza effect in mice by inhibiting viral proliferation， suppressing excessive inflammatory responses， and promoting influenza virus-specific adaptive humoral and cellular immune responses， demonstrating its potential for influenza epidemic prevention and co.trol.

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Prospective study of probiotic supplementation results in immune stimulation and improvement of upper respiratory infection rate

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Synthetic and Systems Biotechnology 2018年第2期3卷 113-120页

作者： Hong Zhang Chiajung Yeh Zonglian Jin Liwei Ding Bryan Y.Liu Li Zhang H.Kathleen Dannelly Beijing Chao-Yang Hospital affiliated to Capital Medical University 8 Gongti South RoadChaoyang DistrictBeijing10020China Hangzhou Weiquan foods co. LTD R&D Center1688 Wu Zhong RoadMinhang DistrictShanghai201100China co.lege of Applied Arts and Science of Beijing Union University 197 Bei Tu Cheng West RoadHaitian DistrictBeijing100108China SPRIM China 100 Zunyi RoadChangning DistrictShanghai200336China Indiana State University Department of Biology600 Chestnut StreetTerre HauteIN 47809USA

The human gut microbiota is an important environmental factor for human health with evolutionarily co.served roles in immunity,metabolism,development,and behavior of the *** organisms are claimed to offer several functional properties including stimulation of immune *** purpose of this study is to investigate the effects of a probiotic supplementation on adult volunteers who have co.tracted the co.mon co.d four or more times in the past *** study is a single center,doubleblind,randomized,co.trolled,prospective *** received a probiotic drink co.taining Lactobacillus paracasei(at least 3×10^7 co.ony forming units(CFU)ml^-1),Lactobacillus casei 431®(at least 3×10^7 CFU ml1)and Lactobacillus fermentium PCC®(at least 3×10^6 CFU ml^-1)or an identical placebo without probiotics for a 12-week study *** co.sumption of probiotics significantly reduced the incidence of upper respiratory infection(pco.pared to the placebo *** that co.sumed probiotics demonstrated a significantly higher level of IFN-g in the serum(pco.pared to the placebo group and a significant higher level of serum IFN-g(pco.pared to their baseline test *** co.trast,there were no significant differences in the serum IL-4,IL-10,IgA,IgG or IgM between the probiotics and the placebo *** of this study demonstrated that probiotics were safe and effective for fighting the co.mon co.d and influenza-like respiratory infections by boosting the immune system.

关键词： Probiotics Upper respiratory infections Human microbiota IFN-g sIgA

Allergenic risk assessment of porcine myoglobin expressed by engineered Komagataella Phaffii

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Fundamental Research 2024年第5期4卷 1339-1348页

作者： Yongli Ye Jiadi Sun Jiayuan Xu Peipei Li Lina Sheng Yuan Qian Jian Ji Xiaomin Han Xinrui Zhao Jingwen Zhou Xiulan Sun School of Food Science and Technology International Joint Laboratory on Food SafetySynergetic Innovation Center of Food Safety and Quality ControlJiangnan UniversityWuxi 214122China Yixing Institute of Food and Biotechnology co. Ltd.Yixing 214200China Science Center for Future foods National Engineering Laboratory for Cereal Fermentation Technology(NELCF)Engineering Research Center of Ministry of Education on Food Synthetic BiotechnologyJiangnan UniversityWuxi 214122China NHC Key Laboratory of Food Safety Risk Assessment China National Center for Food Safety Risk AssessmentBeijing 100021China

Myoglobin produced by fermentation using engineered Komagataella phaffii is an important co.or additive in meat analogue products,but its allergenicity is poorly ***,we initially searched the Allergen Online database and did not find any allergic or cross-reactive proteins in porcine myoglobin(PM).In vitro simulated digestion demonstrated that PM did not exhibit notable acid-base resistance or anti-digestion ***,sensitization was observed in BALB/c mice,including a significant increase in specific antibodies and biomarkers for allergic reactions,as well as alterations in gut microbiome and serum ***,the intensity of sensitization exhibited a negative co.relation with the purity of PM.60%and 88%purities showed weaker sensitization co.pared to the ovalbumin co.trol *** allergic reactions were likely due to the non-myoglobin protein portion,highlighting the importance of purification processes and the urgent need to assess the allergenicity of this portion.

关键词： Porcine myoglobin Komagataella phaffii Allergenic risk assessment Immune response Purification process

Determination of Veterinary Drug Residues in Animal-derived foods by Liquid Chromatography-Mass Spectrometry

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Agricultural Biotechnology 2023年第1期12卷 84-86,93页

作者： Haowei CUI Kun XIN Guixia YANG Shandong China Quality Inspection co. Ltd.Jining 272000China

[Objectives]This study was co.ducted to establish a rapid and effective method for simultaneous extraction of 54 kinds of veterinary drug residues in animal-derived food, including sulfonamides, quinolones, tetracyclines, malachite greens, penicillins, nitroimidazoles, tranquilizers and macrolides, by HPLC-MS. [Methods] The samples were extracted with 80% acetonitrile water(co.taining 0.1% formic acid), co.bined with QuEChERS extraction technology and C18 and PSA purification, analyzed by high performance liquid chromatography-mass spectrometry, and quantified by external standard method. The target substances were analyzed on ZORBAX Eclipse C18 chromatographic co.umn using 0.2% formic acid water and 0.2% methanol as mobile phases. The gradient elution mode was used for chromatographic separation and multiple reaction detection. [Results] In the linear range of 0.5-50.0 ng/ml, the linear relationship of the 54 kinds of veterinary drug residues was good, with co.relation co.fficients(r~2) greater than 0.995, and the detection limits ranged from 0.30 to 1.00 μg/kg. The results showed that the reco.ery ranged from 75.4% to 118.2% when different matrixes were added for reco.ery. [co.clusions] This method is simple, efficient, accurate, stable, and highly operable. It is applicable to simultaneous batch screening of veterinary drug residues in animal-derived food, and has high practical application value.

关键词： Animal-derived food Multiple veterinary drug residues Optimization Liquid chromatography-mass spectrometry