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Systematic Characterization of Cell Cycle Phase-dependent Protein Dynamics and Pathway Activities by High-content Microscopy-assisted Cell Cycle Phenotyping

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Genomics, Proteomics & Bioinformatics 2014年第6期12卷 255-265页

作者： Christopher Bruhn Torsten Kroll Zhao-Qi Wang Leibniz Institute for Age Research – Fritz Lipmann Institute (FLI) IFOM – FIRC Institute of Molecular Oncology Faculty of Biology and Pharmacy Friedrich Schiller University of Jena

Cell cycle progression is coordinated with metabolism, signaling and other complex cellular functions. The investigation of cellular processes in a cell cycle stage-dependent manner is often the subject of modern molecular and cell biological research. Cell cycle synchronization and immunostaining of cell cycle markers facilitate such analysis, but are limited in use due to unphysiological experimental stress, cell type dependence and often low flexibility. Here, we describe high-content microscopy-assisted cell cycle phenotyping（hi MAC）, which integrates high-resolution cell cycle profiling of asynchronous cell populations with immunofluorescence microscopy. hi MAC is compatible with cell types from any species and allows for statistically powerful, unbiased, simultaneous analysis of protein interactions, modifications and subcellular localization at all cell cycle stages within a single sample. For illustration, we provide a hi MAC analysis pipeline tailored to study DNA damage response and genomic instability using a 3–4-day protocol,which can be adjusted to any other cell cycle stage-dependent analysis.

关键词： hi MAC Non-invasive cell cycle assay Cell cycle profiling DNA damage markers Imaging

知识管理的成功因素-一个实证研究现状的综合分析

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科研管理 2004年第Z1期25卷 133-139页

作者： R.Meckl R.Helm University of Bayreuth Friedrich Schiller University Jena

知识管理领域的15年国际研究过程中,虽然出现了大量的相关概念、建议及特定的实践模型,然而关于知识管理的成功因素至今还没有达成共识。本研究通过中间分析法,收集了大量的关于知识管理方面的研究,目的是找出成功进行知识管理的一系列... 详细信息

知识管理领域的15年国际研究过程中,虽然出现了大量的相关概念、建议及特定的实践模型,然而关于知识管理的成功因素至今还没有达成共识。本研究通过中间分析法,收集了大量的关于知识管理方面的研究,目的是找出成功进行知识管理的一系列决定因素,并给出关于成功因素特征的相关建议。

关键词：知识管理综合分析实践研究成功因素

Contemporary climate infuence on variability patterns of Anadenanthera colubrina ***,a key species in seasonally dry tropical forests

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Journal of Forestry Research 2022年第1期33卷 89-101页

作者： María Victoria García María Eugenia Barrandeguy Kathleen Prinz Genética de Poblaciones y Cuantitativa-Departamento de Genética-Facultad de Ciencias Exactas Químicas y Naturales-Universidad Nacional deMisionesInstituto de Biología Subtropical(UNaM-CONICET)Nodo PosadasFélix de Azara 15523300 PosadasArgentina Consejo Nacional de Investigaciones Científicas Y Técnicas(CONICET) PosadasMisionesArgentina Institute of Systematic Botany With Herbarium Haussknecht and Botanical Gardens Friedrich-Schiller-University Jena07743 JenaGermany

The distribution of many plant species has been shaped by climate changes,and their current phenotypic and genetic variability refect microclimatically suitable *** study relates contemporary climate to variability patterns of phenotypic traits and molecular markers in the Argentinean distribution of Anadenanthera colubrina ***,as well as to identify the most relevant phenotypic trait or molecular marker associated with those *** from four populations in both biogeographic provinces,Paranaense and Yungas,were *** analyses and multiple linear regressions were carried out to determine relationships among phenotypic traits and nuclear microsatellites,respectively,to climatic variables,and to identify the phenotypic traits as well as nuclear microsatellite loci most sensitive to *** and three clusters of individuals were detected based on genetic and phenotypic data,*** clusters based on genetic data refected the biogeographic origin of *** traits were the most relevant indicators of climatic *** microsatellite locus Ac41.1 appeared to be non-neutral presenting a strong correlation with climate variable temperature *** findings show complex patterns of genetic and phenotypic variability in the Argentinean distribution of *** *** related to the present or contemporary climate,and provides an example for an integrative approach to better understand climate impact on contemporary genetic and phenotypic variability in light of global climate change.

关键词： Contemporary climate Curupay Genetic variability Phenotypic variability Seasonally dry tropical forests

Nanoscopic Tip Sensors Fabricated by Gas Phase Etching of Optical Glass Fibers

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Photonic Sensors 2012年第4期2卷 331-339页

作者： Jorg BIERLICH Jens KOBELKE David BRAND Konstantin KIRSCH Jan DELLITH Hartmut BARTELT IPHT Institute of Photonic Technology Albert-Einstein-Sir. 9 07745 Jena Germany EAH University of Applied Sciences Carl-Zeiss-Promenade 2 07745 Jena Germany

Silica-based fiber tips are used in a variety of spectroscopic, micro- or nano-scopic optical sensor applications and photonic micro-devices. The miniaturization of optical sensor systems and the technical implementation using optical fibers can provide new sensor designs with improved properties and functionality for new applications. The selective-etching of specifically doped silica fibers is a promising method in order to form complex photonic micro structures at the end or within fibers such as tips and cavities in various shapes useful for the all-fiber sensor and imaging applications. In the present study, we investigated the preparation of geometrically predefined, nanoscaled fiber tips by taking advantage of the dopant concentration profiles of highly doped step-index fibers. For this purpose, a gas phase etching process using hydrofluoric acid （HF） vapor was applied. The shaping of the fiber tips was based on very different etching rates as a result of the doping characteristics of specific optical fibers. Technological studies on the influence of the etching gas atmosphere on the temporal tip shaping and the final geometry were performed using undoped and doped silica fibers. The influence of the doping characteristics was investigated in phosphorus-, germanium-, fluorine- and boron-doped glass fibers. Narrow exposed as well as protected internal fiber tips in various shapes and tip radiuses down to less than 15 nm were achieved and characterized geometrically and topologically. For investigations into surface plasmon resonance effects, the fiber tips were coated with nanometer-sized silver layers by means of vapour deposition and finally subjected to an annealing treatment.

关键词： Optical fiber tips nanoprobes gas phase etching doping tip shaping metal coating

Enhancement of the laser-driven proton source at PHELIX

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High Power Laser Science and Engineering 2020年第2期8卷 153-160页

作者： J.Hornung Y.Zobus P.Boller C.Brabetz U.Eisenbarth T.Kühl Zs.Major J.B.Ohl M.Zepf B.Zielbauer V.Bagnoud GSI Helmholtzzentrum fur Schwerionenforschung GmbH Planckstraße 164291 DarmstadtGermany Friedrich-Schiller-Universitat Fürstengraben 107743 JenaGermany Helmholtz-Institut jena Frobelstieg 307743 JenaGermany Technische Universitat Darmstadt Karolinenplatz 564289 DarmstadtGermany Johannes Gutenberg University MainzSaarstraße 2155122 MainzGermany

We present a study of laser-driven ion acceleration with micrometre and sub-micrometre thick targets,which focuses on the enhancement of the maximum proton energy and the total number of accelerated particles at the PHELIX *** laser pulses with a nanosecond temporal contrast of up to 10^-12 and an intensity of the order of 1020 W/cm^2,proton energies up to 93 MeV are ***,the conversion efficiency at 45°incidence angle was increased when changing the laser polarization to p,enabling similar proton energies and particle numbers as in the case of normal incidence and s-polarization,but reducing the debris on the last focusing optic.

关键词： high power laser proton acceleration secondary sources

A Survey on Some Anisotropic Hardy-Type Function Spaces

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Analysis in Theory and Applications 2020年第4期36卷 373-456页

作者： Jun Liu Dorothee D.Haroske Dachun Yang School of Mathematics China University of Mining and TechnologyXuzhouJiangsu 221116China Institute of Mathematics Friedrich Schiller University JenaJena 07737Germany Laboratory of Mathematics and Complex Systems(Ministry of Education of China) School of Mathematical SciencesBeijing Normal UniversityBeijing 100875China

Let A be a general expansive matrix on *** aims of this article are *** first one is to give a survey on the recent developments of anisotropic Hardy-type function spaces on Rn,including anisotropic Hardy–Lorentz spaces,anisotropic variable Hardy spaces and anisotropic variable Hardy–Lorentz spaces as well as anisotropic Musielak–Orlicz Hardy *** second one is to correct some errors and seal some gaps existing in the known *** unsolved problems are also presented.

关键词： Expansive matrix (variable)Hardy space (variable)Hardy–Lorentz space Musielak–Orlicz Hardy space.

Oxytocin and Stress Response

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Open Journal of Endocrine and Metabolic Diseases 2018年第3期8卷 93-104页

作者： Abeer E. Dief Elena V. Sivukhina Gustav F. Jirikowski Department of Medical Physiology University of Alexandria Alexandria Egypt Institute für Anatomie II Friedrich Schiller Universit&#228 t jena Jena Germany

In response to a stressful unexpected experience, the brain activates a complex stress system that involves the organism in an adaptive response to the threatening situation. This stress system acts on several peripheral tissues and feeds back to the brain. One of its key players is oxytocin hormone. The neuropeptide, oxytocin (OT), has well-established roles during parturition and lactation. In addition to its peripheral actions, OT is released within multiple areas of the brain and influences behavioural and neuroendocrine responses to stress. Several studies suggest that oxytocin is implicated in the central control of responses to stress through modulation of corticotrophin releasing hormone (CRH). Intranasal OT application was associated with an inhibitory effect on adrenocorticotrophic hormone (ACTH) secretion and subsequent impairment of corticosterone secretion. This may be of importance for understanding and perhaps suggesting its utility to buffer stress. Synthesis and release of OT depend to a great extent on steroid hormones particularly on estradiol and corticosterone. Estrogens stimulate synthesis and release of OT and increase the number of OT receptors in some areas of the brain. However, the role of OT in mediating stress is variable and may also depend on gender and on external factors.

关键词： Oxytocin Central Stress Response Corticotrophin Releasing Hormone Steroid Hormone Hypothalamic-Pituitary-Adrenal Axis

Domestication and crop evolution of wheat andbarley: Genes, genomics, and future directions

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Journal of Integrative Plant Biology 2019年第3期61卷 204-225页

作者： Matthew Haas Mona Schreiber Martin Mascher Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben Palaeogenetics Group Institute of Organismic and Molecular Evolution Johannes Gutenberg University Mainz German Center for Integrative Biodiversity Research (iDiv) Halle-jena-Leipzig

Wheat and barley are two of the founder crops of the agricultural revolution that took place 10,000 years ago in the Fertile Crescent and both crops remain among the world's most important crops. Domestication of these crops from their wild ancestors required the evolution of traits useful to humans, rather than survival in their natural environment. Of these traits, grain retention and threshability, yield improvement, changes to photoperiod sensitivity and nutritional value are most pronounced between wild and domesticated forms. Knowledge about the geographical origins of these crops and the genes responsible for domestication traits largely pre-dates the era of nextgeneration sequencing, although sequencing will lead to new insights. Molecular markers were initially used to calculate distance(relatedness), genetic diversity and to generate genetic maps which were useful in cloning major domestication genes. Both crops are characterized by large,complex genomes which were long thought to be beyond the scope of whole-genome sequencing. However, advances in sequencing technologies have improved the state of genomic resources for both wheat and barley. The availability of reference genomes for wheat and some of its progenitors,as well as for barley, sets the stage for answering unresolved questions in domestication genomics of wheat and barley.

关键词： Domestication Wheat barley

Treasures in insect collections:the future of the bomb-radiocarbon analysis

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Insect Science 2018年第6期25卷 1128-1131页

作者： Norbert Kunert Alida Mercado Cárdenas Biogeochemical Processes Max-Planck-lnsUtute for BiogeochemistryHans-Knoll-Str.10JenaGermany Chair of Silviculture Universitɑt FreiburgTennenbacherstr.4FreiburgGermany

Dear Editor, Bomb-radiocarbon analysis of modern carbon has been applied in many fields of *** method allows de- terrnining the residence time of carbon in photosynthetic products and thus aU organic matter in *** atmospheric 14C signature will soon drop below the pre- bomb level due to fixation of atmospheric carbon in ter- restrial and marine ecosystems and high rates of lac-free fossil fuel ***,the time is running out for bomb-radiocarbon *** collections shelter an immense quantity of specimens stored in *** show how those specimens can be used for exploring the trophic structure of food webs by comparing their isotopic radiocarbon signature.

关键词： ATMOSPHERIC RADIOCARBON CARBON