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PIWI proteins and their interactors in piRNA biogenesis,germline development and gene expression

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National Science Review 2014年第2期1卷 205-218页

作者： Hsueh-Yen Ku Haifan Lin Yale Stem cell Center and Department of cell Biology Yale University School of Medicine

PIWI-interacting RNAs(piRNAs) are a complex class of small non-coding RNAs that are mostly 24–32nucleotides in length and composed of at least hundreds of thousands of species that speciically interact with the PIWI protein subfamily of the ARGONAUTE family. Recent studies revealed that PIWI proteins interact with a number of proteins, especially the TUDOR-domain-containing proteins, to regulate piRNA biogenesis and regulatory function. Current research also provides evidence that PIWI proteins and pi RNAs are not only crucial for transposon silencing in the germline, but also mediate novel mechanisms of epigenetic programming, DNA rearrangements, m RNA turnover, and translational control both in the germline and in the soma. hese new discoveries begin to reveal an exciting new dimension of gene regulation in the cell.

关键词： PIWI piRNA TDRD epigenetic RNA decay translational regulation

Enzymatic properties of phenoloxidase from Pieris rapae （Lepidoptera） larvae

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Insect Science 2006年第4期13卷 251-256页

作者： CHAO-BIN XUE WAN-CHUN LUO QING-XI CHEN QIN WANG LI-NA KE College of Plant Protection Shandong Agricultural University Shandong Tai'an The Key lab of Ministry of Education for cell Biology and Tumor cell Engineering School of Life Sciences Xiamen University Fujian Xiamen China

The kinetic parameters of partially purified phenoloxidase （PO, EC. 1.***.18.1） from the 5th instar larvae of Pieris rapae （Lepidoptera） were determined, using L-3, 4- dihydroxyphenylalanine （L-DOPA） as substrate. The optimal pH and temperature of the enzyme for the oxidation of L-DOPA were determined to be at pH 7.0 and at 42℃, respectively. The enzyme was stable between pH 6.5 and 7.4 and at temperatures lower than 37℃. At pH 6.8 and 37℃, the Michaelis constant （Kin） and maximal velocity （V） of the enzyme for the oxidation of L-DOPA were determined to be 0.80 mmol/L and 1.84 μmol/ L/min, respectively. Tetra-hexylresorcinol and 4-dodecylresorcinol effectively inhibited activity of phenoloxidase and this inhibition was reversible and competitive, with the IC50 of 1.50 and 1.12 μmol/L, respectively. The inhibition constants were estimated to be 0.50 and 0.47μmol/L, respectively.

关键词： inhibitory mechanism Pieris rapae phenoloxidase properties 4-hexylresorcinol 4-dodecylresorcinol

Production of translocation by crossing two different alien substitutions in wheat

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Chinese Science Bulletin 1999年第14期44卷 1294-1297页

作者： Yiwen Li Shunxue Tang Tiehan Zhao Hui Liang Hongjie Li Shuang’e Jia Zhensheng Li Xu Jia Acad Sinica Inst Genet State Key Lab Plant Cell & Chromosome Eng Beijing 100101 Peoples R China

The translocation frequency up to 3.7% has been obtained by crossing a Triticum-Thinopyrum substitution with a Triticum-Hayna/dia substitution. The pattern of translocations includes not only wheat-alien Robertson translocations but also inserting translocations involving Thinopyrum chromatin and Haynaldia chromatin. The result indicates that it may be a new method of producing translocations to make two different wheat-alien substitution cross with each other.

关键词： substitution hybridization translocation genomic in situ hybridization.

Booming cancer immunotherapy fighting tumors

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Science China(Life Sciences) 2017年第12期60卷 1445-1449页

作者： Dan Li Wei Wang lab of Gene and cell Therapy Cancer CenterCollaborative Innovation Center for BiotherapyWest China HospitalSichuan UniversityChengdu610041China.

In 2013,cancer immunotherapy led the annual breakthroughs in *** fact,immunotherapy is one of the hotspots in cancer therapy,and can date back to the Coley vaccine in *** in biotechnology and bio-engineering,especially research and development（R＆D）in the fields of therapeutic antibodies,checkpoint inhibitors,and engineered T cells,helped overcome the problems in tumor therapies,leading to increasing research focus on cancer immunotherapy.

关键词： Booming cancer immunotherapy fighting tumors

Influence of alloplasm on resistance to wheat scab

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Chinese Science Bulletin 1998年第12期43卷 1038-1041页

作者： Wu Yuwen Ren Shuxin Liu Chunguang Hou Ning Zhang Cuilan Zhang Yan Chinese Acad Sci Inst Genet State Key Lab Plant Cell & Chromosome Engineer Beijing 100101 Peoples R China

Using alloplasmic wheat as test materials, the genetic effect of cytoplasm on wheat scab resistance was studied by three methods including the determination of resistance of explant calli to Gibberella zeae toxin, the identification of inoculating single fixed position and the identification of field resistance. The results showed that among "the homonucleo_heterocytoplasmic lines", resistance of calli to G. zeae toxin was different; the difference of cytoplasmic effect among different nucleoplasm combinations showed certain nucleoplasm interaction relation. Resistance of hybrid F 1 plants of reciprocal cross between alloplasmic line and disease-sensitive wheat variety to wheat scab was of clear difference. Hybrid F 1 of reciprocal cross between Ae. ventricosa cytoplasmic wheat and disease-sensitive or -resistant wheat variety all exhibited fine resistance effect of alloplasm. Compared with general common wheat variety, most alloplasmic wheats tested have stronger resistance. A few selected lines having Ae. ventricosa cytoplasm have a strong and stable resistance to scab. They can be a finer new resistant source.

关键词： wheat scab resistant genetic cytoplasmic effect.

Multifaceted roles of ASB proteins and its pathological significance

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Frontiers in Biology 2018年第5期13卷 376-388页

作者： Vivek Vishnu Anasa Palaniyandi Ravanan Priti Talwar Apoptosis and cell Survival Research lab Department of Biosciences School of Biosciences and Technology VIT University Vellore Tamil Nadu 632014 lndia

BACKGROUND： Post-translational （PT） modification in cells regulates many intracellular events like signal transduction, transcription, cell cycle, protein quality control, apoptosis and cellular development. Ubiquitination is one of the PT modifications which ftmctions as a marker for degradation of target proteins by the proteasome and as a regulatory mechanism for several signalling pathways. The ubiquitination mechanism requires multiple enzymes, including El, E2, and E3 ligases. Among them, E3 ligases play a major role in recognizing target proteins and an essential feature of protein homeostatic mechanisms within the cell. Most of the ASB （ankyrin repeat SOCS box） proteins fimction as RING family of E3 ubiquitin ligases characterized by the presence of two conserved domains N-terminal ankyrin repeat and C-terminal SOCS box domain METHODS and RESULTS： Current studies have shown that some ASBs function as important regulators of several signalling pathways. This review gives an overview of ASB proteins on numerous cellular processes such as insulin signalling, spermatogenesis, myogenesis and in cellular development. Including various pathological situations, such as cancer, primary open-angle glaucoma, and inflammation, indicating that ASBs has important functions in both normal and pathological development CONCLUSIONS： This article provides a precise comprehensive focus on ASBs protein structure, its biological functions, and their pathological significance.

关键词： ankyrin repeat SOCS box E3 ligase cancer spermatogenesis cellular development

Aberrant expression of nuclear matrix proteins during HMBA-induced differentiation of gastric cancer cells

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World Journal of Gastroenterology 2010年第17期16卷 2176-2182页

作者： Jing, Guang-Jun Xu, Dong-Hui Shi, Song-Lin Li, Qi-Fu Wang, San-Ying Wu, Fu-Yun Kong, Hai-Yan Xiamen Univ Key Lab Minist Educ Cell Biol & Tumor Cell Engn Sch Life Sci Xiamen 361005 Fujian Province Peoples R China Xiamen Univ Dept Hepat Biliary Pancreat Vasc Surg Affiliated Hosp 1 Xiamen 361003 Fujian Province Peoples R China

AIM: To investigate the aberrant expression of nuclear matrix proteins in human gastric cancer cells before and after hexamethylene bisacetamide (HMBA) ***: Proteomics analysis of differential nuclear matrix proteins was performed by two dimensional electrophoresis polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass *** expression levels of three nuclear matrix proteins were further confirmed by Western blotting and their locations in nuclear matrix filament were observed by quantum dots-based ***: Proteomics analysis showed that 43 protein spots were significantly changed due to HMBA *** proteins were identified in the HMBAinduced differentiation of gastric tumor *** proteins spots were down-regulated while seven were *** these proteins,prohibitin,nucleophosmin and hnRNP A2/B1 were significantly decreased in HMBA-treated human gastric cancer cells,and their locations in nuclear matrix were altered by *** results proved the alteration of specific nuclear matrix proteins during the differentiation of human gastric cancer *** the aberrant expressions of nuclear matrix proteins were of significance in revealing the regulatory mechanism of tumor cell proliferation and ***: The aberrant expressions and intracellular redistributions of nuclear matrix proteins before and after HMBA treatment indicated that nuclear matrix proteins play a pivotal role in the differentiation of gastric cancer cells.

关键词： Human gastric tumor cell Hexamethylene bisacetamide Differentiation Nuclear matrix Proliferation

Construction,expression and tumor targeting of a single-chain Fv against human colorectal carcinoma

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World Journal of Gastroenterology 2003年第4期9卷 726-730页

作者： Jin Fang Hong-Bin Jin Jin-Dan Song Key lab of cell Biology,Ministry of Public Health of China,China Medical University,Shenyang 110001,Liaoning Province,China Key lab of cell Biology Ministry of Public Health of China China Medical University 92 Beier Road Heping District Shenyang 110001 Liaoning Province China.

AIM: A single-chain antibody fragment, ND-1scFv, against human colorectal carcinoma was constructed and expressed in ***, and its biodistribution and pharmacokinetic properties were studied in mice bearing ***: VH and VL genes were amplified from hybridoma cell IC-2, secreting monoclonal antibody ND-1, by RT-PCR,and connected by linker (Gly4Ser)3 to form scFv gene, which was cloned into expression vector pET 28a(+) and finally expressed in ***. The expressed product ND-1scFv was purified by metal affinity chromatography using Ni-NTA, its purity and biological activity were determined using SDSPAGE and ELISA. ND-1scFv was labeled with 99mTc, and then injected into mice bearing colorectal carcinoma xenograft for phamacokinetic study in ***: SDS-PAGE analysis showed that the relative molecular weight of recombinant protein was 30kDa with purity of 94%. ELIAS assay revealed that ND-1scFv retained the immunoactivity of parent mAb, being capable of binding specifically to human colorectal carcinoma cell line expressing associated antigen. Radiolabeled ND-1scFv exhibited rapid tumor targeting, with specific distribution in mice bearing colorectal carcinoma xenograft observed as early as 1 h following injection. In vivo pharmacokinetic studies also demonstrated that ND-1scFv had very rapid plasma clearance (T1/2α of 5.7 min, T1/2β of 2.6 h).CONCLUSION: ND-1scFv shows significant immunoactivity,and better pharmacokinetic and biodistribution characteristics compared with intact mAbs, demonstrating the possibility as a carrier for tumor-imaging.

关键词：结直肠癌肿瘤靶向治疗单链抗体 ND-1scFv 单克隆抗体免疫吸附试验

Tributyrin inhibits human gastric cancer SGC-7901 cell growth by inducing apoptosis and DNA synthesis arrest

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World Journal of Gastroenterology 2003年第4期9卷 660-664页

作者： Jun Yan Yong-Hua Xu lab of Molecular and cellular Oncology and lab of Molecular cell Biology,Institute of Biochemistry and cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China lab of Molecular and cellular Oncology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue Yang Road Shanghai 200031 China.

AIM: To evaluate the effects of tributyrin, a pro-drug of natural butyrate and a neutral short-chain fatty acid triglyceride, on the growth inhibition of human gastric cancer SGC-7901 ***: Human gastric cancer SGC-7901 cells were exposed to tributyrin at 0.5, 1, 2, 5, 10 and 50 mmol·L-^1 for 24-72h. MTT assay was applied to detect the cell proliferation.[^3H]-TdR uptake was measured to determine DNA *** morphology was observed by electron microscopy and Hoechst-33258 staining. Flow cytometry and terminal deoxynudeotidyl transferase-mediated dUTP nick end labeling(TUNEL) assay were performed to detect tributyrin-triggered apoptosis. The expressions of PARP, Bcl-2 and Bax were examined by Western blot ***: Tributyrin could initiate growth inhibition of SGC-7901 cell in a dose-and time-dependent manner. [^3H]-TdR uptake by SGC-7901 cells was reduced to 33.6% after 48h treatment with 2mmol·L^-1 tributyrin, compared with the control (Pcells in dose-dependent manner. After 48 hours incubation with tributyrin at 2mmol·L^-1, the level of Bcl-2 protein was lowered, and the level of Bax protein was increased in SGC-7901, accompanied by PARP ***: Tributyrin could inhibit the growth of gastric cancer cells effectively in vitro by inhibiting DNA synthesis and inducing apoptosis, which was associated with the downregulated Bcl-2 expression and the up-regulated Bax expression. Therefore, tribublrin might be a promising chemopreventive and chemotherapeutic agent against human gastric carcinogenesis.

关键词：丁酸甘油酯胃癌细胞凋亡 DNA合成抑制 SGC-7901细胞系化学疗法