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Genome Analysis of the Bombyx mori Infectious Flacherie Virus Isolated in China

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Agricultural Sciences in China 2010年第2期9卷 299-305页

作者： LI Ming-qian CHEN Xiao-xue WU Xiao-xia MAN Na-na JIN Wei LU Xing-meng laboratory of invertebrate Pathology College of Animal Sciences Zhejiang University Hangzhou 310029 P.R.China

We reported here the complete genome of the Bombyx mori infectious flacherie virus （BmlFV）, BmlFV-CHN01, isolated in China and compared it with the Japanese strain IFV （BmIFV-JAN） sequence. BmIFV-JAN and BmIFV-CHN01 were 99% identical in nucleotide and amino acid sequences. The amino acid sequences of the three structural proteins （VP1, VP3 and VP4） and L protein were 100% identical between the two strains. Phylogenetic analyses based on conserved domains in RNA-dependent RNA polymerases （RdRps） by the neighbor-joining method showed that these two strains were from the same virus. The gain of the whole genome of the BmIFV isolated in China and its weak mutation character established a great foundation to the study of functional genome and the molecular epidemiology of BmIFV.

关键词： Bombyx mori infectious flacherie virus complete genome phylogenetic analysis

The larvae of Gnaptorina felicitana and Agnaptoria amdoensis of the tribe Blaptini from China(Coleoptera:Tenebrionidae)

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动物分类学报 2014年第2期39卷 275-282页

作者： Xi-Chao Zhu Guo-Dong Ren College of Life Sciences of Hebei University Key Laboratory of Invertebrate Systematics and Application of Hebei Province

This paper describes the morphological data of the larvae of two species from the tribe Blaptini Leach, 1815. The two species are Gnaptorina felicitana Reitter and Agnaptoria amdoensis G. Medvedev, whose larvae have not been reported up to now. In this paper, morphological characters, informations of specimen, and character figures of the two species＇ larvae are provided. A key to the two genera and the reported species is listed here. The larvae specimens are deposited in the Museum of Hebei University.

关键词： Coleoptera Tenebrionidae Blaptini larva.

Species of the genus Otacilia from Hainan Island,China(Araneae:Phrurolithidae)

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Zoological Systematics 2015年第4期40卷 436-450页

作者： Li-Na Fu Jing-Chao He Feng Zhang The Key laboratory of invertebrate Systematics and Application College of Life SciencesHebei University

Five spider species of the genus Otacilia Thorell, 1897 from Hainan Island are reported, including two species described as new to science, O. biarclata sp. nov. and O. florifera sp. nov. and three species reported, O. bawangling Fu, Zhang ＆ Zhu, 2010, O. jianfengling Fu, Zhang ＆ Zhu, 2010, and O. limushan Fu, Zhang ＆ Zhu, 2010. The male of O. limushan is reported here for the first time. Morphological illustrations and descriptions of new species and O. limushan are presented, and photos of all species are provided.

关键词： spider taxonomy Otacilia China.

Genetic Improvement of Willow for Bioenergy and Biofuels

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Journal of Integrative Plant Biology 2011年第2期53卷 151-165页

作者： Angela Karp Steve J.Hanley Sviatlana O.Trybush William Macalpine Ian Shield Centre for Bioenergy and Climate Change Plant and Invertebrate Ecology DepartmentRothamsted Research

Willows （Salix spp.） are a very diverse group of catkin-bearing trees and shrubs that are widely distributed across temperate regions of the globe. Some species respond well to being grown in short rotation coppice （SRC） cycles, which are much shorter than conventional forestry. Cop- picing reinvigorates growth and the biomass rapidly accumulated can be used as a source of renewable carbon for bioenergy and biofuels. As SRC willows re-distribute nutrients during the perennial cycle they require only minimal nitrogen fertilizer for growth. This results in fuel chains with potentially high greenhouse gas reductions. To exploit their potential for renewable energy, willows need to be kept free of pests and diseases and yields need to be improved without significantly increasing the requirements for fertilizers and water. The biomass composition needs to be optimized for different end-uses. Yields also need to be sustainable on land less productive for food crops to reduce conflicts over land use. Advances in understanding the physiology and growth of willow, and in the identification of genes underlying key traits, are now at the stage where they can start to be used in breeding programs to help achieve these goals.

关键词： Genetic Improvement of Willow for Bioenergy and Biofuels QTLs SRC

Genetic Modification of Baculovirus Expression Vectors

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Virologica Sinica 2012年第2期27卷 71-82页

作者： Shu-fen Li Hua-lin Wang Zhi-hong Hu Fei Deng State Key laboratory of Virology and Joint-Lab of invertebrate Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China

As a protein expression vector, the baculovirus demonstrates many advantages over other vectors. With the development of biotechnology, baculoviral vectors have been genetically modified to facilitate high level expression of heterologous proteins in both insect and mammalian cells. These modifications include utilization of different promoters and signal peptides, deletion or replacement of viral genes for increasing protein secretion, integration of polycistronic expression cassette for producing protein complexes, and baculovirus pseudotyping, promoter accommodation or surface display for enhancing mammalian cell targeting gene delivery. This review summarizes the development and the current state of art of the baculovirus expression system. Further development of baculovirus expression systems will make them even more feasible and accessible for advanced applications.

关键词： Baculovirus Protein expression Promoters Signal peptides Gene delivery

An efficient method of constructing homologous recom binant baculovirus with PCR-amplified fragments

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Science China(Life Sciences) 2003年第4期46卷 431-437页

作者：侯松旺陈新文王汉中胡志红 Joint-lab of invertebrate Virologyand Key laboratory of Molecular Virology WuhanInstitute of Virology Chinese Academy of Sciences Keylaboratory of Agricultural Microbiology Ministry of Agriculture HuazhongAgricultureUniversityWuhan430072China

This paper describes a rapid method of constructing homologous recombinant baculovirus in E. coli with PCR-amplified fragments. By using this method, the traditional steps of constructing transfer vector are omitted. The method is based on phage l red system which can promote the recombination between the homologous fragments with the length above 36 bp. Taking HaSNPV as an example, this paper describes the rapid recombination process by using chloramphenicol resistance gene (CmR) to replace orf135 in HaSNPV genome. A pair of primers with length of 60 bp was synthesized, in which 40 bp was homologous to the each end sequence of orf135, and the rest 20 bp was homologous to the each end sequence of CmR. By using these primers, a linear fragment containing the complete CmR gene between 40 bp of homologous arms of orf135 was generated by PCR with the plasmid pKD3 which contains CmR as the template. By transforming the linear fragment into the E. coli containing the bacterial artificial chromosome of HaSNPV and with the help of a plasmid expressing l recombinase, the recombinants on which the homologue replacement had taken place were selected by chloramphenicol resistance. This method greatly shortens the process of constructing recombinant baculovirus since the process was performed in E. coli and does not need to construct transfer vectors. It can be further used for gene replacement and gene deletion of other large viral genomes.

关键词： phage l red system baculovirus Bacmid gene replacement gene deletion HaSNPV recombinant virus.

Phylogenomic approaches in systematic studies

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Zoological Systematics 2020年第3期45卷 151-162页

作者： Junxia Zhang Jiaxing Lai The Key laboratory of invertebrate Systematics and Application Institute of Life Science and Green DevelopmentCollege of Life SciencesHebei UniversityBaoding 071002HebeiP.R.China

Phylogenomic approaches are growingly applied in the systematic studies of animals with the rapid advancement of sequencing and analytical *** to accelerate the systematic studies using genome-scale data in China,this paper outlines the commonly used techniques for generating phylogenomic datasets:RNA-seq based transcriptome,targetenrichment based UCEs and AHE,RAD-seq,whole genome sequencing,and briefly discusses the pros and cons of each *** analytical procedures for the 1 KITE transcriptome pipeline,the PHYLUCE(UCEs)and AHE pipelines and the PLWS(low-coverage whole genome sequencing)pipeline are summarized,and recent achievements valuable for phylogenomic analyses are introduced.

关键词： Transcriptome UCEs AHE RAD-seq WGS

What is the difference between marine and limnetic-terrestrial associations of nematodes with invertebrates?

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Integrative Zoology 2022年第4期17卷 481-510页

作者： Alexei V.TCHESUNOV Viatcheslav N.IVANENKO Department of invertebrate Zoology Faculty of BiologyM.V.Lomonosov Moscow State UniversityMoscowRussia

Zoo-and phyto-parasitic nematodes of the order Rhabditida and zooparasites of the subclass Dorylaimia are well known,due largely to their medical,veterinarian and agricultural ***,there have been many switches from a free-living to a symbiotic(including parasitism)mode of existence in the evolutionary trajectories of various nematode ***,we attempt to summarize all known cases of symbioses(from commensalism to true parasitism)between marine nematodes representing nonparasitic taxa and various larger animals,ranging from protists to *** cases are of nematodes relating to dwelling on crustaceans(ectocommensalism)or living in the body cavity and internal organs of various invertebrates(endoparasitism or parasitoidism).Ectocommensal species may differ from their free-living relatives in their longer filiform bodies and enlarged ventral and caudal glands,which may be interpreted as adaptations for the purpose of fixation on the body surface of a motile *** species are characterized by deep anatomical modification,including rudimentation of the alimentary tract and hypertrophy of reproductive *** terrestrial and limnetic invertebrates,marine invertebrates have almost no intestinal nematode *** evolutionary trajectories of nematode dwellers of marine and nonmarine invertebrates are compared.

关键词： commensalism marine invertebrates Nematoda parasitism symbiosis

Happy together？ Avoidance of conspecifics by gregarious mussels

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Current Zoology 2018年第1期64卷 53-61页

作者： Anna DZIERZYNSKA-BIALONCZYK Aleksandra SKRZYPCZAK Jaroslaw KOBAK Faculty of Biology and Environmental Protection Department of Invertebrate Zoology Nicolaus CopernicusUniversity Lwowska 1 Torun 87-100 Poland

Zebra mussel Dreissena polymorpha is a Ponto-Caspian species invasive in Europe and North America, with great environmental impact. It lives byssally attached to hard substrata in large aggregations, which is often explained by its preferences for conspecifics, though direct evidence for such preferences has been rather limited so far. We studied the reactions of zebra mussels to con- specifics, hypothesizing that they may either be attracted to one another or form aggregations only in the absence of alternative attachment sites, in Experiment 1, we tested mussel tendency to detach from existing druses depending on druse size （2-25 individuals） and substratum type （soft： sand; hard： glass）. Mussels detached significantly more often on the hard substratum and from larger druses compared to soft substratum and smaller druses, respectively. This indicates that mussels tended to avoid conspecifics at high density, particularly when alternative substratum was available. In Experiment 2, we tested the responses of single mussels to distant （3 or 15cm） conspecifics （0, 3, 15 individuals per 2.51 tank） on the sandy substratum. The presence of conspecifics, regardless of their distance and density, resulted in single unattached mussels staying more often in their initial positions. Mussels did not move preferentially towards or away from the conspecifics. Thus, even on unsuitable substratum mussels were not attracted by conspecifics and probably exhibited an avoidance reaction by reducing their movement. This suggests that dense mussel aggregations are formed due to the lack of available alternative attachment sites rather than due to their preferences for conspecifics.

关键词： active detachment Dreissena polymorpha movement mussel aggregations zebra mussel.