检索结果-南通市图书馆

Insect Science 2022年第4期29卷 1120-1134页

作者： Xin An Wei Zhang Chao Ye Guy Smagghe Jin-Jun Wang Jinzhi Niu Key laboratory of Entomology and pest control Engineering College of Plant ProtectionSouthwest UniversityChongqingChina international joint laboratory of china–belgium on sustainable crop pest control Academy of Agricultural SciencesSouthwest UniversityChongqingChina Department of Plants and crops Faculty of Bioscience EngineeringGhent UniversityGhentBelgium

Aphids are important agricultural pests,vectors of many plant viruses and have sophisticated relationships with symbiotic microorganisms.Abundant asymptomatic RNA viruses have been reported in aphids due to the application of RNA-seq,but aphid-virus interactions remain unclear.Bunyavirales is the most abundant RNA virus order,which can infect mammals,arthropods,and plants.However,many bunyaviruses have specific hosts,such as insects.Here,we discovered 18 viruses from 10 aphid species by RNA-seq.Importantly,a widespread presence bunyavirus,Aphid bunyavirus 1(ABV-1),was determined to have a wide host range,infecting and replicating in all 10 tested aphid species.ABV-1 may be transmitted horizontally during feeding on plant leaves and vertically through reproduction.In a comparison of the physiological parameters of ABV-1high and ABV-1low strains of pea aphid,higher ABV-1 titers reduced the total nymphal duration and induced the reproduction.Moreover,viral titer significantly affected the lipid and protein contents in pea aphids.In summary,we proposed that ABV-1 may have stable symbiont-like relationships with aphids,and these observations may provide a new direction for studying bunyaviruses in aphids and establishing a model for virus-aphid interactions.

关键词： insec-specific virus pea aphid Phasmaviridae RNA virus RNAi

Characterization of carotenoid biosynthetic pathway genes in the pea aphid(Acyrthosiphon pisum)revealed by heterologous complementation and RNA interference assays

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2022年第3期29卷 645-656页

作者： Bi-Yue Ding Xiu-Cheng Xie Feng Shang Guy Smagghe Jin-Zhi Niu Jin-Jun Wang Key laboratory of Entomology and pest control Engineering College of Plant ProtectionSouthwest UniversityChongqingChina international joint laboratory of china-belgium on sustainable crop pest ControlAcademy of Agricultural SciencesSouthwest UniversityChongqingChina Department of Plants and crops Faculty of Bioscience EngineeringGhent UniversityGhentBelgium

Carotenoids are involved in many essential physiological functions and are produced from geranylgeranyl pyrophosphate through synthase,desaturase,and cyclase activities.In the pea aphid(Acyrthosiphon pisum),the duplication of carotenoid biosynthetic genes,including carotenoid synthases/cyclases(ApCscA-C)and desaturases(ApCdeA-D),through horizontal gene transfer from fungi has been detected,and ApCdeB has known dehydrogenation functions.However,whether other genes contribute to aphid carotenoid biosynthesis,and its specific regulatory pathway,remains unclear.In the current study,functional analyses of seven genes were performed using heterologous complementation and RNA interference assays.The bifunctional enzymes ApCscA-C were responsible for the synthase of phytoene,and ApCscC may also have a cyclase activity.ApCdeA,ApCdeC,and ApCdeD had diverse dehydrogenation functions.ApCdeA catalyzed the enzymatic conversion of phytoene to neurosporene(three-step product),ApCdeC catalyzed the enzymatic conversion of phytoene to ζ-carotene(two-step product),and ApCdeD catalyzed the enzymatic conversion of phytoene to lycopene(four-step product).Silencing of ApCscs reduced the expression levels of ApCdes,and silencing these carotenoid biosynthetic genes reduced the α-,β-,and γ-carotene levels,as well as the total carotenoid level.The results suggest that these genes were activated and led to carotenoid biosynthesis in the pea aphid.

关键词： carotenoid biosynthesis heterologous complementary pea aphid RNA interference

Molting process revealed by the detailed expression profiles of RXR1/RXR2 and mining the associated genes in a spider mite, Panonychus citri

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2022年第2期29卷 430-442页

作者： Gang Li Xun-Yan Liu Guy Smagghe Jin-Zhi Niu Jin-Jun Wang Key laboratory of Entomology and pest control Engineering College of Plant ProtectionInternational Joint Laboratory of China-Belgium on Sustainable Crop Pest ControlAcademy of Agricultural SciencesSouthwest UniversityChongqingChina Provincial Key laboratory for Agricultural pest Management of Mountainous Regions Institute of EntomologyGuizhou UniversityGuiyangChina Department of Plants and crops Faculty of Bioscience EngineeringGhent UniversityGhentBelgium

Spider mites have one ecdysone receptor(EcR)and multiple retinoid X receptors(RXRs).However,the function of these RXRs in spider mite development is unknown.Here,we screened the expression dynamics of two PcRXR isoforms at 4 h intervals in the deutonymphal stage of Panonychus citri.The results showed that PcEcR had an expression pattern similar to that of PcRXR2.For PcRXR1,its expression remained at a certain high level,when there was a decrease of both PcEcR and PcRXR2.In situ hybridization showed that PcRXR2 was detected in the central nervous mass,while the ecdysteroid biosynthesis gene PcSpo was mainly expressed at the edge of the central nervous mass.RNAi-based silencing of PcRXR1 or PcRXR2 showed the same phenotype as in mites with that of silencing PcEcR.Furthermore,RNA-seq was used to mine the genes associated with the expression dynamics of PcRXR1 or PcRXR2,which revealed that the heterodimer of EcR-RXR2 in spider mites might be linked with the cell autophagy and tissue remodeling during apolysis,and RXR1 might be linked with new epicuticle and exocuticle secretion during ecdysis.Taken together,these results increase our understanding of the regulation mechanism of ecdysteroid signal pathway in spider mite development.

关键词： ecdysone receptor(EcR) in situ hybridization retinoid X receptors RNA interference RNA-seq

CRISPR-mediated mutagenesis of the odorant receptor co-receptor(Orco)gene disrupts olfaction-mediated behaviors in Bactrocera dorsalis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2022年第5期29卷 1275-1286页

作者： Li Xu Hong-Bo Jiang Kai-Yue Tang Ying Yan Marc F.Schetelig Jin-Jun Wang Key laboratory of Entomology and pest control Engineering College of Plant ProtectionSouthwest UniversityChongqingChina International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control Academy of Agricultural SciencesSouthwest UniversityChongqingChina Department of Insect Biotechnology in Plant Protection Justus-Liebig-University GiessenInstitute for Insect BiotechnologyGiessenGermany

Olfaction plays an essential role in insect behavior such as host location,foraging,mating,and oviposition.The odorant receptor co-receptor(Orco)is an obligatory odorant receptor and indispensable in odor perception.Here,we characterized the Orco gene from the oriental fruit fly,Bactrocera dorsalis(Hendel),a notorious agriculture pest.The olfactory deficiency mutants were generated by editing the BdorOrco gene using the CRISPR/Cas9 system.Electroantennograms(EAG)and olfactory preference assays confirmed that BdorOrco^(−/−)mutant flies had reduced perception of methyl eugenol,β-caryophyllene,and ethyl acetate.Oviposition bioassays showed that the eggs laid by BdorOrco^(−/−)females mediated by benzothiazole and 1-octen-3-ol were significantly decreased.In addition,BdorOrco^(−/−)mutant flies took a significantly longer time to locate the food source compared with wild type(WT)flies.Altogether,our data indicated that Orco is essential for multiple physiological processes in B.dorsalis,and it expands our understanding of the function of insect Orco.

关键词： CRISPR/Cas9 foraging olfaction oriental fruit fly oviposition

Characterization of Domeless receptors and the role of Bd Domeless3 in anti-symbiont-like virus defense in Bactrocera dorsalis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2024年第4期23卷 1274-1284页

作者： Wei Zhang Shaoyang Li Rong Li Jinzhi Niu Jinjun Wang Key laboratory of Entomology and pest control Engineering College of Plant ProtectionSouthwest UniversityChongqing 400715China Key laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River Ministry of Education/International Joint Laboratory of China-Belgium on Sustainable Crop Pest ControlAcademy of Agricultural SciencesSouthwest UniversityChongqing 400715China

The Janus kinase/signal transducers and activators of transcription(JAK/STAT)signaling pathway play a pivotal role in innate immunity.Among invertebrates,Domeless receptors serve as the key upstream regulators of this pathway.In our study on Bactrocera dorsalis,we identified three cytokine receptors:BdDomeless1,BdDomeless2,and BdDomeless3.Each receptor encompasses five fibronectin-type-III-like(FN III)extracellular domains and a transmembrane domain.Furthermore,these receptors exhibit the increased responsiveness to diverse pathogenic challenges.Notably,only BdDomeless3 is upregulated during symbiont-like viral infections.Moreover,silencing BdDomeless3 enhanced the infectivity of Bactrocera dorsalis cripavirus(BdCV)and B.dorsalis picorna-like virus(BdPLV),underscoring BdDomeless3’s crucial role in antiviral defense of B.dorsalis.Following the suppression of Domeless3 expression,six antimicrobial peptide genes displayed decreased expression,potentially correlating with the rise in viral infectivity.To our knowledge,this is the first study identifying cytokine receptors associated with the JAK/STAT pathway in tephritid flies,shedding light on the immune mechanisms of B.dorsalis.

关键词： Bactrocera dorsalis JAK/STAT pathway Domeless receptors antiviral immunity symbiont-like virus

Identification and characterization of odorant binding proteins in the diamondback moth,Plutella xylostella

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Insect Science 2021年第4期28卷 987-1004页

作者： Li-Jun Cai Li-Shuang Zheng Yu-Ping Huang Wei Xu Min-Sheng You State Key laboratory of Ecological pest control for Fujian and Taiwan crops Institute of Applied EcologyFujian Agriculture and Forestry UniversityFuzhouChina joint international Research laboratory of Ecological pest control Ministry of EducationFuzhouChina Key laboratory of Integrated pest Management for Fujian-Taiwan crops Ministry of AgricultureFuzhouChina Agricultural Sciences College of ScienceHealthEngineering and EducationMurdoch UniversityMurdochAustralia

Odorant binding proteins(OBPs)are a group of soluble proteins functioning as odorant carriers in insect antennae,mouth parts and other chemosensory organs.However,multiple insect OBPs have been detected in other tissues and various functions have been proposed.Therefore,a detailed expression profile including stages,tissues and sexes where OBPs are expressed will assist in building the links to their potential functions,enhancing the functional studies of insect OBPs.Here,we identified 39 putative OBP genes from its genome and transcriptome sequences of diamondback moth(DBM),Plutella xylostella.The expression patterns of identified PxylOBPs were further investigated from eggs,larvae,pupae,virgin adults,mated adults,larval midgut,larval heads,adult antennae,adult heads and adult tarsi.Moreover,P.xylostella larvae and adults with and without host plants for 5 h were utilized to study the interactions between OBP expression and host plants.The results showed that most PxylOBPs were highly expressed in male and female adult antennae.The expression levels of certain PxyOBPs could be regulated by mating activities and feeding host plants.This study advances our knowledge of P.xylostella OBPs,which may help develop new strategies for more environmentally sustainable management of P.xylostella.

关键词： gene expression insect olfaction OBP qRT-PCR

CRISPR/Cas9-based functional analysis of yellow gene in the diamondback moth,Plutella xylostella

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2021年第5期28卷 1504-1508,I0002页

作者： Yajun Wang Yuping Huang Xuejiao Xu Zhaoxia Liu Jianyu Li Xue Zhan Guang Yang Minsheng You Shijun You State Key laboratory of Ecological pest control for Fujian-Taiwan crops Institute of Applied EcologyFujian Agriculture and Forestry UniversityFuzhou 350002China joint international Research laboratory of Ecological pest control Ministry of EducationFuzhou 350002China Key laboratory of Integrated pest Management for Fujian-Taiwan crops Ministry of AgricultureFuzhou 350002China ADepartment of Physiology&Neurobiology University of ConnecticutStorrsCT 06269USA Fujian Key laboratory for Monitoring and Integrated Management of crop pests Institute of Plant ProtectionFujian Academy of Agricultural SciencesFuzhou 350013China

The diamondback moth,Plutella xylostella(L.),is an economically important pest of cruciferous crops worldwide.This pest is notorious for rapid evolution of the resistance to diferent classes of insecticides,making it increasingly dificult to control.Genetics-based control approaches,through manipulation of target genes,have been reported as promising supplements or alternatives to traditional methods of pest management.Here we identified a gene of pigmentation(yellow)in P.xylostella,Pxyellow,which encodes 1674 bp complementary DNA sequence with four exons and three introns.Using the clustered regularly interspersed palindromic repeats(CRISPR)CRISPR-associated protein 9 system,we knocked out Pxyellow,targeting two sites in Exon III,to generate 272 chimeric mutants(57%of the CRISPR-treated individuals)with color-changed phenotypes of the Ist to 3rd instar larvae,pupae,and adults,indicating that Pxyellow plays an essential role in the body pigmentation of P xlostella.Fitness analysis revealed no significant difference in the oviposition of adults,the hatchability of eggs,and the weight of pupac between homozygous mutants and wildtypes,suggesting that Pxyellow is not directly involved in regulation of growth,development,or reproduction.This work advances our understanding of the genetic and insect science molecular basis for body pigmentation of P xylostella,and opens a wide avenue for development of the genctcally based pest control techniques using Pxyellow as a screening marker.

关键词： CRISPR/Cas9 diamondback moth genetically based control novel marker yellow gene

Structure-guided analysis of Arabidopsis JASMONATE-INDUCED OXYGENASE(JOX)2 reveals key residues for recognition of jasmonic acid substrate by plant JOXs

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2021年第5期14卷 820-828页

作者： Xin Zhang Dongli Wang Joyce Elberse Linlu Qi Wei Shi You-Liang Peng Robert C.Schuurink Guido Van den Ackerveken Junfeng Liu State Key laboratory of Agrobiotechnology Ministry of Agriculture Key Laboratory for Crop Pest Monitoring and Green ControlJoint International Research Laboratory of Crop Molecular BreedingCollege of Plant ProtectionChina Agricultural UniversityBeijing 100193China Plant-Microbe Interactions Department of BiologyUtrecht University3508 TB Utrechtthe Netherlands Green Life Sciences Research Cluster Swammerdam Institute for Life SciencesUniversity of Amsterdam1098 XH Amsterdamthe Netherlands

The jasmonic acid(JA)signaling pathway is used by plants to control wound responses.The persistent accumulation of JA inhibits plant growth,and the hydroxylation of JA to 12-hydroxy-JA by JASMONATE-INDUCED OXYGENASEs(JOXs,also named jasmonic acid oxidases)is therefore vital for plant growth,while structural details of JA recognition by JOXs are unknown.Here,we present the 2.65Åresolution X-ray crystal structure of Arabidopsis JOX2 in complex with its substrate JA and its co-substrates 2-oxoglutarate and Fe(Ⅱ).JOX2 contains a distorted double-stranded p helix(DSBH)core flanked by a helices and loops.JA is bound in the narrow substrate pocket by hydrogen bonds with the arginine triad R225,R350,and R354 and by hydrophobic interactions mainly with the phenylalanine triad F157,F317,and F346.The most critical residues for JA binding are F157 and R225,both from the DSBH core,which interact with the cyclopentane ring of JA.The spatial distribution of critical residues for JA binding and the shape of the substrate-binding pocket together define the substrate selectivity of the JOXs.Sequence alignment shows that these critical residues are conserved among JOXs from higher plants.Collectively,our study provides insights into the mechanism by which higher plants hydroxylate the hormone JA.

关键词： crystal structure JASMONATE-INDUCED OXYGENASEs(JOXs) jasmonic acid(JA) 12-OH-JA hydroxylation

Delaying application time of slow-release fertilizer increases soil rhizosphere nitrogen content,root activity,and grain yield of spring maize

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The crop Journal 2022年第6期10卷 1798-1806页

作者： Guanghao Li Pengxiao Fu Guigen Cheng Weiping Lu Dalei Lu Jiangsu Key laboratory of crop Genetics and Physiology/Jiangsu Key laboratory of crop Cultivation and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain crops Yangzhou UniversityYangzhou 225009JiangsuChina joint international Research laboratory of Agriculture and Agri-Product Safety the Ministry of Education of ChinaYangzhou UniversityYangzhou 225009JiangsuChina

Application of slow-release fertilizer(SF)is a nutrient-management measure aimed at improving maize nutrient use and yield and saving labor cost.One-time application of SF at sowing usually results in nutrient deficiency during the post-silking stage,owing to the long growth period of spring maize.This study was conducted to investigate the effects on spring maize of SF application stage(zero,three-,and six-leaf stages,designated as SF0,SF3,and SF6,respectively)on grain yield,total soil rhizosphere nitrogen(N)content,and root activity,in comparison with the conventional fertilization mode(CF,application of compound fertilizer at sowing time,and topdressing urea at six-leaf and tasseling stages)at the same fertilization level as the control.Compared with no fertilization(F0)and CF,SF increased grain number and weight.The maize cultivars Suyu 30(SY30)and Jiangyu 877(JY877)produced the highest grain yield and net return under SF6 treatment over the three years.SF6 increased enzymatic activities including oxidoreductase,hydrolase,transferase,and lyase in rhizosphere soil at silking(R1)and milking stages(R3).SF6 increased the total N contents of rhizosphere soil by 7.1%at R1 and 9.2%R3 stages compared with SF0.The activities of antioxidant enzymes in roots were increased under SF6 treatments at R1and R3.The mean root activities of SF0,SF3,and SF6 increased by 7.1%,12.8%,and 20.5%compared with CF at R1 and by 8.8%,13.0%,and 23.5%at R3.Delaying the application time of SF could increase grain yield by increasing total N content of rhizosphere soil,delaying root senescence,and increasing root activity at the late reproductive stage.Applying SF at the six-leaf stage is recommended as an effective fertilization strategy for the sustainable production of spring maize in southern china.

关键词： Spring maize Slow-release fertilizer Fertilization stage Root activity Grain yield

Mechanisms for flowering plants to benefit arthropod natural enemies of insect pests： Prospects for enhanced use in agriculture

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Insect Science 2014年第1期21卷 1-12页

作者： Zhong-Xian Lu Ping-Yang Zhu Geoff M. Gurr Xu-Song Zheng Donna M. Y. Read Kong-Luen Heong Ya-Jun Yang Hong-Xing Xu State Key laboratory Breeding Base for Zhejiang sustainable pest and Disease control Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Sciences Hangzhou China EH Graham Centre for Agricultural Innovation Charles Stuff University Orange NSW Australia College of Chemistry and Life Science Zhejiang Normal University Jinhua Zhejiang Province China and crop and Environmental Sciences Division International Rice Research Institute Los Banos Metro Manila Philippines

Reduction of noncrop habitats, intensive use of pesticides and high levels of disturbance associated with intensive crop production simplify the farming landscape and bring about a sharp decline of biodiversity. This, in turn, weakens the biological control ecosystem service provided by arthropod natural enemies. Strategic use of flowering plants to enhance plant biodiversity in a well-targeted manner can provide natural enemies with food sources and shelter to improve biological control and reduce dependence on chemical pesticides. This article reviews the nutritional value of various types of plant-derived food for natural enemies, possible adverse effects on pest management, and the practical application of flowering plants in orchards, vegetables and field crops, agricultural systems where most research has taken place. Prospects for more effective use of flowering plants to maximize biological control of insect pests in agroecosystem are good but depend up on selection of optimal plant species based on information on the ecological mechanisms by which natural enemies are selectively favored over pest species.

关键词： conservation biological control ecological engineering habitat nectar,nutrition pollen shelter