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Gene expression changes in spleens of the wildlife reservoir species,Eurasian wild boar(Sus scrofa),naturally infected with Brucella suis biovar 2

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Journal of Genetics and Genomics 2010年第11期37卷 725-736页

作者： Ruth C.Galindo Pilar M.Muoz María J.de Miguel Clara M.Marin Javier Labairu Miguel Revilla José M.Blasco Christian Gortazar José de la Fuente instituto de investigación en Recursos Cinegéticos IREC(CSIC-UCLM-JCCM) Unidad de Sanidad Animal Centro de Investigación y Tecnología Agroalimentaria(CITA) ITG Ganadero Avda. Serapio Huici 2231610 VillabaNavarraSpain departamento de Patología Animal Facultad de VeterinariaUniversity of Zaragoza department of Veterinary Pathobiology Center for Veterinary Health SciencesOklahoma State University

Brucella suis is responsible for swine brucellosis worldwide. Of the five different B. suis biovars （by.）, bv. 2 appears restricted to Europe where it is frequently isolated from wild boar and hares, can infect pigs and can cause human brucellosis. In this study, the differ- ential gene expression profile was characterized in spleens of Eurasian wild boar naturally infected with B. suis bv. 2. Of the 20,201 genes analyzed in the microarray, 633 and 1,373 were significantly （fold change 〉 1.8; P 〈 0.01） upregulated and downregulated, respectively, in infected wild boar. The analysis was focused on genes that were over represented after conditional test for biological process gene ontology. Upregulated genes suggested that B. suis bv. 2 infection induced cell maturation, migration and/or proliferation in infected animals. The genes downregulated in infected wild boar impaired the activity of several important cellular metabolic pathways such as metabolism, cytoskeleton organization and biogenesis, immune response and lysosomal function and vesicle-mediated transport. In addition, the response to stress, sperm fertility, muscle development and apoptosis seemed to be also impaired in infected animals. These results suggested that B. suis bv. 2 may use strategies similar to other smooth brucellae to facilitate intracellular multiplication and the development of chronic infections. To our knowledge, this is the first report of the analysis of gene expression profile in hosts infected with B. suis bv. 2, which is important to understand the molecular mechanisms at the host-pathogen interface in the main reservoir species with possible implications in the zoonotic cycle of the pathogen.

关键词： Brucella suis boar genomics microarray systems biology

Vibrational behavior of isotropic plate structures in contact with a bounded fluid via unified formulation

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Chinese Journal of Aeronautics 2019年第4期32卷 921-937页

作者： F.G.CAnALES J.L.MAnTARI Faculty of Mechanical engineering Instituto de Investigación en Ingeniería Naval(IDIIN)Universidad Nacional de Ingenieria(UNI) department of Mechanical engineering University of New Mexico

This paper presents an analytical solution for free vibration analysis of thick rectangular isotropic plates coupled with a bounded fluid for various boundary conditions. In order to consider displacement theories of an arbitrary order, the Carrera Unified Formulation(CUF) is used. The eigenvalue problem is obtained by using the energy functional, considering plate and fluid kinetic energies as well as the potential energy of the plate. The Ritz method is used to evaluate the displacement variables, and the functions used in the Ritz series can be adjusted to consider any of the classical boundary conditions. The convergence of the solution is analyzed, and a validation of results considering open literature and 3D finite element software is performed. Parametric studies are carried out to obtain natural frequencies as a function of the side-to-thickness ratio, plate aspect ratio, fluid domain size, plate boundary conditions, and fluid-solid density ratio. Pressure and velocity in the fluid domain are evaluated in order to establish the consistency of the *** results for thick plates are obtained with a much lower computational cost compared to that of 3D finite element solutions.

关键词： Added mass Fluid-structure interaction Plate Ritz method Unified formulation Vibration

Coyote(Canis latrans)in South America:potential routes of colonization

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Integrative Zoology 2020年第6期15卷 471-481页

作者： Octavio MOnROy-VILCHIS JoséF.GOnZÁLEZ-MAyA Ángel BALBUenA-SERRAnO Fausto ELVIR Martha M.ZARCO-GOnZÁLEZ Clarita RODRÍGUEZ-SOTO Centro de investigación en ciencias Biológicas Aplicadas Universidad Autónoma del Estado de MéxicoInstituto Literario 100TolucaMéxico50000México instituto de Ecología Universidad Nacional Autónoma de MéxicoCoyoacánMéxico The Sierra to Sea Institute and ProCAT Colombia Las AlturasCoto BrusPuntarenasCosta Rica Fundación investigación en ciencias para el Estudio y Conservación de la Biodiversidad Instituto de Ciencia para el Estudio y Conservación de la Biodiversidadfrente a la Escuela República de JapónTegucigalpaHonduras Facultad de Geografía Universidad Autónoma del Estado de MéxicoInstituto Literario 100TolucaMéxico50000México

During the last century,the coyote(Canis latrans)has increased its distribution in Central *** the 1980s,it had not been recorded in *** records show that coyotes have crossed the Panama Canal,indicating that continues to expand;therefore,there is a possibility that it will reach northern South *** objectives were to identify potential coyote colonization routes to South America,and the variables that favor its *** hypothesized that habitat fragmentation benefits coyote *** applied 7 algorithms to model the potential distribution of the coyote,using 196 presence records and 12 *** models with better performance were used to generate a consensus *** our consensus model and the areas with highest probability of presence,a potential colonization route was generated between Central America and northern South *** route lies through southern Costa Rica,along the Pacific coast of Panama to the south,to the Andean mountains in northern *** variables that explained potential coyote distribution were human population density,altitude,and percentage of crops with positive influence,and tropical broadleaf forests with negative *** results indicate that human activities and deforestation are related to coyote distribution *** can be implemented within the identified route to improve environmental management,in order to avoid the presence of the coyote in the ecosystems of northern South America.

关键词： Canis latrans carnivore distribution expansion invasive management species distribution model

Caerulin-induced pancreatitis in rats: Histological and genetic expression changes from acute phase to recuperation

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World Journal of Gastroenterology 2006年第25期12卷 3999-4003页

作者： Javier Magaa-Gómez Guillermo López-Cervantes Ana María Calderón de la Barca departamento de nutrición Centro de investigación en Alimentación y desarrollo A.C. HermosilloSonoraMéxicoDepartamento de Patología Hospital Infantil del Estado de SonoraHermosilloSonoraMéxicoDepartamento de Nutrición Centro de Investigación en Alimentación y DesarrolloA.C.HermosilloSonoraMéxico

AIM： To study the histological and pancreatitis-associated protein mRnA accumulation changes of pancreas from acute phase of caerulin-induced pancreatitis to recuperation in rats. METHODS： Acute pancreatitis was induced by caerulein in male Wistar rats and followed up for 90 d by histological and mRnA analyses of pancreas. Pancreases were dissected at 0, 9, 24 h and 3, 5, 15, 30, 60, 90 d post-induction. Edema （E）, polymorphonuclear neutrophU （pIVln） infiltration, cytoplasmic vacuolization （V）, zymogen granule depletion （ZD） and acinar disorganization （AD） were microscopically evaluated. Accumulation of pancreatitis- associated protein （PAP） and L13A mRnAs were quantified by real-time PCR. RESULTS： The main histological changes appeared at 9 h post-induction for PMn infiltration and cytoplasmic V, while at 24 h and 3 d for E and ZD, respectively. All the parameters were recovered after 5 d, except for ZD which delayed more than 30 d. The main AD was observed after 15 d and values returned to normal after 30 d. Similarly to histological changes, accumulation of the PAP mRnA was increased at 9 h with the highest accumulation at 24 h and differences disappeared after 5 d. COnCLUSIOn： From the acute phase to recuperation of pancreatitis, regeneration and re-differentiation of pan- creas occur and PAP expression is exclusively an acute response of pancreatitis.

关键词： Acute pancreatitis Histological changes PAP mRnA L13A mRnA

Variability in mentum deformities of Tanytarsus larvae(Diptera,Chironomidae)in a metal rich basin in northern Mexico

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Water Biology and Security 2022年第2期1卷 1-9页

作者： Orestes Carlos Bello-Gonzalez Perla Alonso-EguíaLis Cesar Arturo Ilizaliturri Hernandez norman Mercado-Silva Centro de investigacion en Biodiversidad y Conservacion Universidad Autonoma del Estado de MorelosCuernavacaMorelosMexico instituto Mexicano de tecnología del Agua JiutepecMorelosMexico Facultad de Medicina-Centro de investigacion Aplicada en Ambiente y Salud Universidad Autonoma de San Luis PotosíCoordinacion para la Aplicacion de la Ciencia y la Tecnología-(CIACyT-CIAAS)San Luis PotosíMexico

Mentum deformities in chironomids have been commonly used as indicators of metal contamination in freshwater *** of these deformities suggests sublethal effects and can provide early signals of environmental *** anthropogenic metal pollution is known to lead to an increase in deformities in chironomids,natural background deformity incidence information is key to their effective use as biomonitoring *** we explore the incidence of deformities from 5000 Tanytarsus(Diptera,Chironomidae)menta in relation to water physicochemical,and sediment metal(Al,As,Cu,Fe,Mn,Pb,Zn)concentration data from numerous sites in the naturally metal-rich Sonora River(nW Mexico)obtained over a two-year *** metal concentrations were found in the upper *** salinity,total suspended solids and conductivity were found in the lower *** As and Cu were occasionally found above published threshold effect levels(TEL).The proportion of deformities(%D)was low(2.1%)and a generalized linear model only explained 25.36%of%D variability among samples;this model included several physicochemical *** only metal significantly related to deformities was *** significant spatial and temporal variability in all metal concentrations and other physicochemical parameters in the basin,we were unable to explain a strong relationship between metal concentration and deformity *** results,based on consistent%D quantification from 5000 samples from a single taxon,suggest that natural variation in metal concentrations,even when statistically significant,is not associated with notable variations in the incidence of deformities.

关键词： Macroinvertebrate Pollution Sonora river desert river Copper Arsenic

Long-term monitoring reveals the effect of precipitation and silviculture on nothofagus regeneration in northern Patagonia mixed forests

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Ecological Processes 2024年第2期13卷 103-117页

作者： Georgina Sola Camila Mateo Alejandro dezzotti Paula Marchelli Hernán Attis Beltrán Renato Sbrancia Luis Chauchard Marcelo González Pe?alba Martín Lara Verónica El Mujtar Consejo nacional de investigaciones Científicas y Técnicas (COnICET ) Asentamiento Universitario San Martín de los Andes (AUSMA-UnCo) instituto de investigaciones Forestales y Agropecuarias Bariloche (IFAB) Instituto Nacional de Tecnología Agropecuaria (INTA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) departamento de Conservación y Manejo Parque Nacional Lanín- Administración de Parques Nacionales (PNL-APN)

Background Tree regeneration is a key component of resilience because it promotes post-disturbance recovery of forests. northwestern Patagonia from Argentina is occupied by nothofagus alpina（na）, n. obliqua（no）, and n. dombeyi（nd） forests that grow along an intense precipitation gradient, managed throughout shelterwood silvicultural system by technicians of the Lanin natural Reserve. The objective of this study was to evaluate the influence of seeding cuttings over the dynamics of nothofagus mixed forests across landscape（precipitation gradient） based mainly on the composition and abundance of tree regeneration, permanent sampling plots and generalized linear mixed models. In particular, we analysed:（i） the structure of sexual and asexual regeneration during nd > 20 years after harvest（the dynamics of managed forests）, and（ii） the structure of sexual regeneration in primary and managed forests after > 20 years of harvest（the effect of silviculture）.Results nd was the most abundant species in the regeneration of managed forests during all periods in both sites despite its lower contribution to the adult cohort. During the 10–20 years period after harvest, the humid site exhibited higher regeneration density than the mesic site(120,000 and 6000 ind ha-1, respectively), and it decreased afterwards. The number of established regeneration（> 2 m height） was lower for na in the mesic site and for no in the humid site(0 and 57 ind ha-1, respectively). However, in comparison to no, na showed a higher number of sprouted stumps and sprouts per stump, and a higher sprout height in the mesic site. no exhibited higher sprout mortality in the humid site. Finally, the regeneration of primary forests showed lower density and height, and a more balanced composition than that of managed *** The silvicultural effects on the mixed forest regeneration dynamics was strongly influenced by the condition of sites. Therefore, management prescriptions should be adj

关键词： nothofagus alpina nothofogus obliqua nothofogus dombeyi Basal sprouting Shelterwood silvicultural system Ecological resilience

The paradigm of protein acetylation in Parkinson's disease

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neural Regeneration Research 2019年第6期14卷 975-976页

作者： Sokhna M.S.yakhine-Diop Guadalupe Martínez-Chacón Elisabet Uribe-Carretero Mireia niso-Santano Rosa A.González-Polo José M.Fuentes Universidad de Extremadura Departamento de Bioquímicay Biología Moleculary GenéticaFacultad de Enfermeríay Terapia Ocupacional Centro de investigación Biomédica en Red en enfermedades neurodegenerativas (CIBERnED) instituto de investigación Biosanitaria de Extremadura (InUBE)

Acetylation is a post-translational modification that is regulated by two antagonistic enzymes,histone acetyltransferases(HATs)and histone deacetylases(HDACs).HATs transfer the acetyl group from acetyl-CoA to lysine residues of proteins while HDACs remove it(yakhine-Diop et al.,2018b).The impairment of HAT or HDAC activity elicits changes in the protein acetylation status which disturb several cellular processes,among others,gene expression,autophagy etc.,leading finally to cell *** enzymes are associated with Parkinson’s disease(PD)*** dopaminergic cells,neurotoxins provoke apoptotic cell death by increasing histone acetylation *** paraquat(Song et al.,2011)and rotenone(Feng et al.,2015)reduce HDAC activity,dieldrin(Song et al.,2010)enhances HAT *** in vivo,paraquat-induced upregulation ofα-synuclein triggers histone hypoacetylation.

关键词： Parkinson protein α-synuclein HDACs cells gene is

Alluvial sedimentation and tectonostratigraphic evolution in a narrow extensional zigzag basin margin (northern Teruel Basin, Spain)

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Journal of Palaeogeography 2019年第4期8卷 368-392页

作者： Lope Ezquerro Aránzazu Luzón José L.Simón Carlos L.Liesa departamento de ciencias de la Tierra Facultad de Ciencias Geotransfer Research Group and Instituto de Investigación en Ciencias Ambientales (IUCA) Universidad de Zaragoza

The northern part of the eastern margin of the extensional neogene Teruel Basin(central-eastern Spain)consists of a non-linear,zigzag fault zone made of alternating ca.2 km long,nnW-SSE trending segments and shorter nnESSW *** outcrop conditions made possible a comprehensive integrated stratigraphic and structural study,especially focused on coarse clastic sediments deposited along the basin ***-exposed stratal relationships with boundary faults,allowed the analysis of tectonic influence on *** deformation includes growth faulting,rollover anticlines,and monoclines and associated onlap stratal terminations,angular unconformities,and other complex growth strata *** of them is the onlap-over-rollover bed arrangement described here for the first time,which reveals the competition between tectonic subsidence and sedimentary ***,the structural inheritance(dense Mesozoic fracture grid)and the dominant,nearly‘multidirectional’(σ1 vertical,σ2≈σ3),Pliocene extensional regime withσ3 close to E-W,are considered to have controlled the margin structure and ***-stratigraphic evolution includes:(i)reactivation of inherited nnW-SSE faults and development of W-SW-directed small alluvial fans(SAF)while nnE-SSW segments acted as gentle relay ramp zones;(ii)progressive activation of nnE-SSW faults and development of nW-directed very small alluvial fans(VSAF);during stages i and ii sediments were trapped close to the margin,avoiding widespread progradation;(iii)linking of nnW-SSE and nnE-SSW structural segments,overall basin sinking and widespread alluvial progradation;(iv)fault activity attenuation and alluvial *** particular structure and kinematic evolution of this margin controlled alluvial system *** of alluvial fans,directly set up at the border faults,was conditioned by the narrowness of the margin,small catchment areas,and proximity between faults,which prevented the development of l

关键词： Extensional basin Alluvial fan Stratal stacking pattern Structural inheritance Fault linkage

Microstructural evaluation and nanohardness of an AlCoCuCrFeniTi high-entropy alloy

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International Journal of Minerals,Metallurgy and Materials 2019年第5期26卷 634-641页

作者： C.D.Gómez-Esparza R.Peréz-Bustamante J.M.Alvarado-Orozco J.Mu?oz-Salda?a R.Martínez-Sánchez J.M.Olivares-Ramírez A.Duarte-Moller Centro de investigación en Materiales Avanzados (CIMAV) Laboratorio Nacional de Nanotecnología COnACyT-Corporación Mexicana de investigación en Materiales S.A.de C.V.(COMIMSA) Centro de Ingeniería y desarrollo Industrial Querétaro Campus Centro de investigación y de Estudios Avanzados del IPn Unidad Querétaro Universidad Tecnológica de San Juan del Río Escuela de Ingeniería Civil Industrialy Mecánica Universidad De La Salle Bajío

An AlCoCuCrFeniTi high-entropy alloy(HEA) was prepared by mechanical alloying and sintering to study the effect of Ti addition to the widely studied AlCoCuCrFeni system. The structural and microstructural characteristics were investigated by X-ray diffraction(XRD), scanning electron microscopy(SEM), and transmission electron microscopy(TEM). The formation of four micrometric phases was detected: a Cu-rich phase with a face-centered cubic(fcc) structure, a body-centered cubic(bcc) solid solution with Cu-rich plate-like precipitates(fcc), an ordered bcc phase, and a tetragonal structure. The XRD patterns corroborate the presence of a mixture of bcc-, fcc-, and tetragonal-structured phases. The Vickers hardness of the alloy under study was more than twice that of the AlCoCuCrFeni alloy. nanoindentation tests were performed to evaluate the mechanical response of the individual phases to elucidate the relationship between chemical composition, crystal structure, and mechanical performance of the multiphase microstructure of the AlCoCuCrFeniTi HEA.

关键词： high-entropy alloys mechanical alloying microstructure nanoindentation