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Plant Communications 2023年第2期4卷 272-285页

作者： Rui Albuquerque-Martins Dora Szakonyi James Rowe Alexander M.Jones Paula Duque instituto gulbenkian de ciencia 2780-156 OeirasPortugal Sainsbury Laboratory University of CambridgeCambridge B21LRUK

Serine/arginine-rich(SR)proteins are conserved splicing regulators that play important roles in plant stress responses,namely those mediated by the abscisic acid(ABA)*** Arabidopsis thaliana SR-like protein SR45 is a described negative regulator of the ABA pathway during early seedling *** the inhibition of growth by ABA signaling is counteracted to maintain plant development under stress conditions remains largely ***,we show that SR45 overexpression reduces Arabidopsis sensitivity to ABA during early seedling *** and confocal microscopy analyses of transgenic plants expressing fluorescently tagged SR45 revealed that exposure to ABA dephosphorylates the protein at multiple amino acid residues and leads to its accumulation,due to SR45 stabilization via reduced ubiquitination and proteasomal *** phosphomutant and phosphomimetic transgenic Arabidopsis lines,we demonstrate the functional relevance of ABA-mediated dephosphorylation of a single SR45 residue,T264,in antagonizing SR45 ubiquitination and degradation to promote its function as a repressor of seedling ABA *** results reveal a mechanism that negatively autoregulates ABA signaling and allows early plant growth under stress via posttranslational control of the SR45 splicing factor.

关键词： abscisic acid alternative splicing Arabidopsis thaliana protein phosphorylation SR proteins

More Transporters,More Substrates:The Arabidopsis Major Facilitator Superfamily Revisited

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Molecular Plant 2019年第9期12卷 1182-1202页

作者： Maria Nino-Gonzalez Esther Novo-Uzal Dale N.Richardson Pedro M.Barros Paula Duque instituto gulbenkian de ciencia 2780-156 OeirasPortugal Genomics of Plant Stress Unit ITQB NOVA-Instituto de Tecnologia Quimica e Biologica Antonio XavierUniversidade Nova de Lisboa2780-157 OeirasPortugal

The Major Facilitator Superfamily(MFS)is ubiquitous in living organisms and represents the largest group of secondary active membrane *** plants,significant research efforts have focused on the role of specific families within the MFS,particularly those transporting macronutrients(C,N,and P)that constitute the vast majority of the members of this *** MFS families remain less explored,although a plethora of additional substrates and physiological functions have been ***,the lack of a systematic approach to analyzing the MFS as a whole has obscured the high diversity and versatility of these ***,we present a phylogenetic analysis of all annotated MFS domaincontaining proteins encoded in the Arab/dops/s fA?a//ana genome and propose that this superfamily of transporters consists of 218 members,clustered in 22 *** reviewing the available information regarding the diversity in biological functions and substrates of Arab/dops/s MFS members,we provide arguments for intensified research on these membrane transporters to unveil the breadth of their physiological relevance,disclose the molecular mechanisms underlying their mode of action,and explore their biotechnological potential.

关键词： Arabidopsis thaliana membrane transport MFS transporters phylogenetic analysis

At the Intersection： Merging Ca2＋ and ROS Signaling Pathways in Pollen

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Molecular Plant 2014年第11期7卷 1595-1597页

作者： Michael M. Wudick José A. Feijó department of Cell Biology and Molecular Genetics University of Maryland College Park MD 20742–5815 USA instituto gulbenkian de ciencia 2780-156 Oeiras Portugal

THE MIXED BLESSING OF ROS： DON＇T YOU KNOW THAT I＇M TOXIC？Due to plants＇ aerobic metabolism, reactive oxygen species （ROS） continuously accrue in different tissues and organs as a by-product of many metabolic reactions. despite their toxic activity, plants also rely on ROS as a major form of second messenger to integrate, induce, and/or propagate biotic and abiotic signals and signaling cascades. Being small, short-lived, and rapidly diffusible makes ROS ideal messenger molecules for local biochemi- cal reactions. Hence, a delicate fine-tuning of localized ROS production and signaling events is crucial for many physi- ologic processes.

关键词： ACTIVE oxygen POLLEN tube CELL membranes CALciUM content of plants HOMOLOGY (Biochemistry) PROTEIN kinases

Energy Signaling in the Regulation of Gene Expression during Stress

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Molecular Plant 2010年第2期3卷 300-313页

作者： Elena Baena-Gonzalez instituto gulbenkian de ciencia Rua da Quinta Grande 6 2780-156 Oeiras Portugal

Maintenance of homeostasis is pivotal to all forms of life. In the case of plants, homeostasis is constantly threatened by the inability to escape environmental fluctuations, and therefore sensitive mechanisms must have evolved to allow rapid perception of environmental cues and concomitant modification of growth and developmental patterns for adaptation and survival. Re-establishment of homeostasis in response to environmental perturbations requires reprog- ramming of metabolism and gene expression to shunt energy sources from growth-related biosynthetic processes to defense, acclimation, and, ultimately, adaptation. Failure to mount an initial ＇emergency＇ response may result in nutrient deprivation and irreversible senescence and cell death. Early signaling events largely determine the capacity of plants to orchestrate a successful adaptive response. Early events, on the other hand, are likely to be shared by different conditions through the generation of similar signals and before more specific responses are elaborated. Recent studies lend credence to this hypothesis, underpinning the importance of a shared energy signal in the transcriptional response to various types of stress. Energy deficiency is associated with most environmental perturbations due to their direct or indirect deleterious impact on photosynthesis and/or respiration. Several systems are known to have evolved for monitoring the available resources and triggering metabolic, growth, and developmental decisions accordingly. In doing so, energy-sensing systems regulate gene expression at multiple levels to allow flexibility in the diversity and the kinetics of the stress response.

关键词： Abiotic/environmental stress metabolic regulation cell signaling gene expression post-transcriptionalcontrol transcriptional control and transcription factors.

XBAT35, a Novel Arabidopsis RING E3 Ligase Exhibiting Dual Targeting of Its Splice Isoforms, Is Involved in Ethylene-Mediated Regulation of Apical Hook Curvature

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Molecular Plant 2012年第6期5卷 1295-1309页

作者： Sofia D. Carvalho Rita Saraiva Teresa M. Maia Isabel A. Abreu Paula Duquea instituto gulbenkian de ciencia Rua da Quinta Grande 6 2780-156 Oeiras Portugal instituto de Tecnologia Quimica e Biologica Universidade Nova de Lisboa Av. da Republica 2780-157 Oeiras Portugal

The Arabidopsis XBAT35 is one of five structurally related ankyrin repeat-containing Really Interesting New Gene （RING） E3 ligases involved in ubiquitin-mediated protein degradation, which plays key roles in a wide range of cel- lular processes. Here, we show that the XBAT35 gene undergoes alternative splicing, generating two transcripts that are constitutively expressed in all plant tissues. The two splice variants derive from an exon skipping event that excludes an in-frame segment from the XBAT35 precursor mRNA, giving rise to two protein isoforms that differ solely in the presence of a nuclear localization signal （NLS）. Transient expression assays indicate that the isoform lacking the NLS localizes in the cytoplasm of plant cells, whereas the other is targeted to the nucleus, accumulating in nuclear speckles. Both isoforms are functional E3 ligases, as assessed by in vitro ubiquitination assays. Two insertion mutant alleles and RNA-interference （RNAi） silencing lines for XBAT35 display no evident phenotypes under normal growth conditions, but exhibit hyper- sensitivity to the ethylene precursor 1-aminocyclopropane-l-carboxylate （ACC） during apical hook exaggeration in the dark, which is rescued by an inhibitor of ethylene perception. Independent expression of each XBAT35 splice variant in the mutant background indicates that the two isoforms may differentially contribute to apical hook formation but are both functional in this ethylene-mediated response. Thus, XBAT35 defines a novel player in ethylene signaling involved in negatively regulating apical hook curvature, with alternative splicing controlling dual targeting of this E3 ubiquitin ligase to the nuclear and cytoplasmic compartments.

关键词： alternative splicing apical hook Arabidopsis thaliana ethylene signaling RiNG E3 ligase ubiquitination.

Targeting of Pollen Tubes to Ovules Is dependent on Nitric Oxide （NO） Signaling

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Molecular Plant 2008年第4期1卷 703-714页

作者： Ana Margarida Prado Renato Colaco Nuno Moreno Ana Catarina Silva Jose A. Feijo instituto gulbenkian de ciencla Centro de Blologia do Desenvolvimento PT-2780-156 Oeiras Portugal Universidade de Lisboa Faculdade de Clencias Dept Biologia Vegetal Campo Grande Ed.C2. PT-1749-016 Lisboa Portugal

The guidance signals that drive pollen tube navigation inside the pistil and micropyle targeting are still, to a great extent, unknown. Previous studies in vitro showed that nitric oxide （NO） works as a negative chemotropic cue for pollen tube growth in lily （Lilium Iongiflorum）. Furthermore, Arabidopsis thaliana Atnosl mutant plants, which show de- fective NO production, have reduced fertility. Here, we focus in the role of NO in the process of pollen-pistil communication, using Arabidopsis in-vivo and lily semi-vivo assays. Cross-pollination between wild-type and Atnosl plants shows that the mutation affects the pistil tissues in a way that is compatible with abnormal pollen tube guidance. Moreover, DAF- 2DA staining for NO in kanadi floral mutants showed the presence of NO in an asymmetric restricted area around the micropyle. The pollen-pistil interaction transcriptome indicates a time-course-specific modulation of transcripts of AtNOS1 and two Nitrate Reductases （nrl and nr2）, which collectively are thought to trigger a putative NO signaling pathway. Semivivo assays with isolated ovules and lily pollen further showed that NO is necessary for micropyle targeting to occur. This evidence is supported by CPTIO treatment with subsequent formation of balloon tips in pollen tubes facing ovules. Activation of calcium influx in pollen tubes partially rescued normal pollen tube morphology, suggesting that this pathway is also dependent on Ca^2＋ signaling. A role of NO in modulating Ca^2＋ signaling was further substantiated by direct imaging the cytosolic free Ca^2＋ concentration during NO-induced re-orientation, where two peaks of Ca^2＋ occur--one during the slowdown/stop response, the second during re-orientation and growth resumption. Taken together, these results provide evidence for the participation of NO signaling events during pollen-pistil interaction. Of special relevance, NO seems to directly affect the targeting of pollen tubes to the ovule＇s micropyle by modu

关键词： pollen tubes NO fertilization guidance calcium signaling

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Molecular Plant 2012年第4期5卷 951-954页

作者： Giuseppe Ortu Raffaella Balestrini Patricia A. Pereira Jorg D. Becker Helge Kuste Paola Bonfante department of Life Science and Systems Biology University of Torino Italy Istituto per la Protezione delle Piante--CNR Torino Italy instituto gulbenkian de ciencia 2780-156 Oeiras Portugal Institut for Pflanzengenetik Leibniz Universitat Hannover Herrenhauser Str. 2 D-30419 Hannover Germany

dear Editor, Phytohormones are essential regulators of plant develop- ment, but their role in the signaling processes between plants and fungi during arbuscular mycorrhizal （AM） establishment is far from being understood （Ludwig-M011er, 2010）. AM coloni- zation leads to extensive effects on host metabolism, as revealed by transcriptome studies of AM plants （Hogekamp et al., 2011）. Some genes have been specified as an AM core set, since they are mycorrhizal-responsive, irrespective of the identity of the plant,

关键词： AM真菌植物激素相关基因早期阶段调节过程生物合成相互作用赤霉素

Modern Built Heritage Conservation Policies: How to Keep Authenticity and Emotion in the Age of Digital Culture

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Built Heritage 2018年第2期2卷 17-34页

作者： Ana Tostões instituto Superior Técnico Universidade de LisboaLisbonPortugal

The Modern Movement has demonstrated its long-term legitimacy as a lasting concept endowed with *** technology,spatial form and social commitment to one another,through an optimistic faith in progress,modern architects sought to attain new heights of functionality and flexibility in *** current challenge is to find ways to deal with the conservation of this recent legacy in the continuously changing context of current times,including physical,economic,functional,and fast-moving socio-cultural and political *** address these questions,‘Modern built heritage conservation policies’explores the restoration and renovation processes undertaken in some paradigmatic case studies:the Tugendhat House(Mies van der Rohe and Lilly Reich,Brno,Czech Republic,1929-1930),the Crown Hall(Mies van der Rohe,Chicago,USA,1950-1956),the National Museum of Western Art(Le Corbusier,Tokyo,Japan,1959)and the gulbenkian Foundation(Alberto Pessoa,Pedro cid e Ruy d’Athouguia,Lisbon,Portugal,1959-1969).

关键词： restoration renovation conservation Modern Movement architecture authenticity materiality adaptive reuse

Does the smartest designer design better？ Effect of intelligence quotient on students＇ design skills in architectural design studio

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Frontiers of Architectural Research 2015年第4期4卷 318-329页

作者： Sajjad Nazidizaji Ana Tome Francisco Regateiro CERIS DECivil Instituto Superior Tecnico Universidude de Lisboa Lisboa 1049-001 Portugal

Understanding the cognitive processes of the human mind is necessary to further learn about design thinking processes. Cognitive studies are also significant in the research about design studio. The aim of this study is to examine the effect of designers intelligence quotient （lOJ on their designs. The statistical population in this study consisted of all deylaman Institute of Higher Education architecture graduate students enrolled in 2011. Sixty of these students were selected via simple random sampling based on the finite population sample size calculation formula. The students＇ IQ was measured using Raven＇s Progressive Matrices. The students＇ scores in Architecture design Studio （ADS） courses from first grade （ADS-l） to fifth grade （ADS-5） and the mean scores of the design courses were used in determining the students＇ design ability. Inferential statistics, as well as correlation analysis and mean comparison test for independent samples with SPSS, were also employed to analyze the research data. Results indicated that the students＇ IQ ADS-1 to ADS-4 scores, and the mean scores of the students＇ design courses were not significantly correlated. By contrast, the students＇ IQ and ADS-5 scores were significantly correlated. As the complexity of the design problem and designers＇ experience increased, the effect of IQ on design seemingly intensified.

关键词： Architectural designstudio Intelligence quotient(IQ) design education Human factors design thinking

Malaria epidemiology in the democratic Republic of East Timor

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Asian Pacific Journal of Tropical Medicine 2010年第4期3卷 283-287页

作者： Afonso de Almeida Virgilio E do Rosario Ana Paula Arez Pedro Cravo Universidade Nacional de Timor Lorosae Avenida Cidade de LisboaDili-Timor Leste Centro de Malaria e Outras Doencas Tropicais Instituto de Higiene e Medicina TropicalUniversidade Nova de LisboaRua da Junqueira 961349-008 Lisboa Portugal Centro de Malaria e Outras Doencas Tropicais Instituto de Higiene e Medicina TropicalUniversidade Nova de LisboaRua da Junqueira 961349008 Lisboa Portugal Unidade de Biologia Molecular Instituto de Higiene e Medicina TropicalUniversidade Nova de LisboaRua da Junqueira 961349-008 Lisboa Portugal

Objective:To investigate malaria prevalence and socio-economic conditions in East Timor. Methods:Blood samples were collected from 650 individuals distributed among six districts in East Timor.434 and 216 individuals were sampled by passive and active case detection, respectively(PCD,ACD).Results:The results showed that the plasmodium infection prevalence was 18.9%with predominance of Plasmodium falciparum(***) infections(60.2%). The majority of cases were detected in children between 2-14 years old in both PCD and ACD. Participants residing in Same and Lospalos were at a higher risk of *** percentage of infections associated to the lack of bed net use was twice as high as users(25.2%vs 12.5%). The prevalence of malaria among participants who lived in brick/cement houses(8.3%) was two times less than those who lived in other types of houses(3.7%).There was a significantly lower prevalence of infection detected in individuals living in complete housing constructions with absence of domestic animals in or near the ***:The major risk factors for malaria in East Timor are age,lack of bed net use,incomplete housing constructions and exposure to mosquitoes.

关键词： East Timor Malaria epidemiology Children Risk factors Housing constructions