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In vitro response of Human Mesenchymal Stromal Cells to Titanium Coated Peek Films and Their Suitability for Magnetic resonance Imaging

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Journal of Materials Science & Technology 2015年第5期31卷 427-436页

作者： Cindy Elschner Carolin Noack Carolin PreilBler Andreas Krause Ulrich Scheler Ute Hempel Leibniz-Institut fr Polymerforschung Dresden e.V. Hohe Str.601069 DresdenGermany CrTD/DFG-Center for regenerative Therapies Dresden Fetscherstr.10501307 DresdenGermany Institut fr physiologische chemie Technische Universitt DresdenFiedlerstr.4201307 DresdenGermany NaMlab gGmbH Nthnitzer Str.6401187 DresdenGermany

Medical imaging is an important tool for the post-operative checkup of an accurate position of an implant as well as for monitoring the integration in the adjacent tissue that may influence the success of a medical ***,the possibility to use imaging methods is associated with the implant material and all the established metallic materials for surgery do not show a proper 'imaging compatibility'.The present study is a combined investigation of the in vitro response to human mesenchymal stromal cells（hMSC） and magnetic resonance imaging（MrI） compatibility of the potential material combination polyetheretherketone/titanium（PEEK/Ti） for medical *** of the advantageous imaging properties and the mechanical and chemical stability,PEEK becomes more and more an alternative to common metallic implant materials like titanium or ***,PEEK is a bioinert material having a limited ability for direct bone *** to its excellent biocompatibility,Ti was chosen as coating material to enhance the cellular *** result is a combination with advantageous properties:the magnetic susceptibility and elastic modulus close to bone,corrosion resistance and mechanical flexibility of PEEK and the excellent biocompatibility of *** appearance of metal-related artifact was discussed in electrical resistivity and magnetic ***,two titanium coatings have been investigated:a complete coating and a structured surface avoiding surface *** determine the in vitro biocompatibility,the cell responses were assessed in terms of the overall morphology of the hMSC and their cell area distribution,proliferation,osteogenic differentiation and mineral *** cellular stress was evaluated by the prostaglandin *** bonded materials both produced no disturbing artifacts in magnetic resonance *** to the pure PEEK material,the titanium coated specimens showed an enhanced biocompatibilit

关键词： Polyetheretherketone Titanium coating Biocompatibi

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深海六放海绵大骨针的结构与特性(英文)

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动物学报 2007年第3期53卷 557-569页

作者：王晓红李锦和乔莉 Heinz C.SCHr DEr Carsten ECKErT Klaus KrOPF 王毅民冯庆玲 Werner E. G. MLLEr 国家地质实验测试中心中国科学院海洋所清华大学材料系 institutfr physiologische chemie Abteilung Angewandte Molekularbiologie Universitat Duesbergweg 6 D-55099 Mainz Germany

在海绵动物(多孔动物)中,六放海绵和寻常海绵为硅质骨骼。生活在深海(1000m)中的六放海绵是最古老的海绵动物,其中间单根海绵和春氏单根海绵有长达3m的骨针,是地球上最长的生物硅结构。利用电子显微技术观测,这些直径达8mm的巨大根须骨... 详细信息

在海绵动物(多孔动物)中,六放海绵和寻常海绵为硅质骨骼。生活在深海(1000m)中的六放海绵是最古老的海绵动物,其中间单根海绵和春氏单根海绵有长达3m的骨针,是地球上最长的生物硅结构。利用电子显微技术观测,这些直径达8mm的巨大根须骨针具有同心层状结构,其横截面显示明显的构造分界:中间为含有轴丝的轴管,外围是一50 -150μm厚的轴筒,最外面为层状区(300 -500层,每层厚度3 -5μm)。生物化学研究显示其主要的蛋白质为35kD大分子,另外,还检测到23 -24kD多肽,可能是硅蛋白相关蛋白。依据现有的红血球凝聚活性,从骨针提取物中也检测到了凝集素。由电子探针获得其化学成分主要为Si , K和Na。此外,骨针的光传输实验表明,该巨大根须骨针用作光纤可传输600nm至1 400nm范围的光,而滤掉小于600nm的光(类似高通滤波器)和大于1 400nm的红外光(类似低通滤波器)。另外,从六放海绵的空囊泡沫海绵中分离出一个基因并确证了其推导的编码蛋白序列,该蛋白编码一个光裂合酶相关蛋白,蛋白相似性比较结果显示属于光裂合酶相关蛋白中多细胞动物隐色素一类。基于以上数据给出了六放海绵硅质骨针形成的示意图。另外,由单根海绵骨针可作为波导传输光/电和/或化学信号,推断在海绵动物中有类似神经系统的网络系统。

关键词：海绵中间单根海绵春氏单根海绵骨针生物硅形成光导硅蛋白相关蛋白纳米生物技术

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Phylogenetic position of sponges in early metazoan evolution and bionic applications of siliceous sponge spicules

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Chinese Science Bulletin 2007年第22期52卷 3029-3040页

作者： Werner E G MULLEr WANG XiaoHong ZENG LingSen Heinz C SCHrODEr Institut for physiologische chemie Abteilung Angewandte Molekularbiologie Universit&t Duesbergweg 6 D-55099 Mainz Germany National research Center for Geoanalysis Beijing 100037 China Faculty for Environmental Sciences Peking University Beijing 100871 China Institute of Geology Chinese Academy of Geological Sciences Beijing 100037 China

Sponges are the oldest and the simplest but not primitive multicellular animals. They represent the earliest evolutionary metazoan phylum still extant. It was a long and painful scientific process to posi-tion the most enigmatic and mysterious metazoan,the Porifera,into their correct phylogenetic place among the eukaryotes in general and multicellular animals in particular. As living fossils,sponges provide the best evidence for the early evolution of Metazoa. More recently,interest has been focused on the bionic applications of sponges' siliceous spicules,after the discovery of their unique structure and high fiber performance. In this review,the emergence of sponges,evolutionary novelties found in sponges,and the phylogenetic position of sponges in early metazoan evolution are highlighted. In ad-dition,the present state of knowledge on silicatein-mediated "biosilica" formation in marine sponges,including the involvement of other molecules in silica metabolism and their potential application in nanobiotechnology and medicine,is given.

关键词：海绵多细胞动物仿生学硅土

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A novel marker for the platyfish (Xiphophorus maculatus) W chromosome is derived from a Polinton transposon

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Journal of Genetics and Genomics 2010年第3期37卷 181-188页

作者： Qingchun Zhou Ingo Braasch Alexander Froschauer Astrid Boehne Christina Schultheis Manfred Schartl Jean-Nicolas Volff Biofuture research Group Physiologische Chemie I Biozentrum University ofWiirzburg Wiirzburg 97074 Germany Hubei Key Laboratory of Genetic regulation and lntegrative Biology College of Life Science Huazhong Normal University Wuhan 97074 China Institut fur Zoologie Technische Universitait Dresden Dresden 01062 Germany Institut de Genomique Fonctionnelle de Lyon Universit~ de Lyon Institut Fdddratif Biosciences Gerland Lyon Sud UniversiM Lyon 1 CNRS INRA Ecole Normale Supdrieure de Lyon Lyon 69364 France

A consensus sequence, encoding a putative DNA polymerase type B derived from a Polinton transposon, was assembled from the sex determination region of Xiphophorus maculatus. This predicted protein, which is 1,158 aa in length, contains a DNAA_pol_B_2 domain and a DTDS motif. The DNA polymerase type B gene has about 10 copies in the haploid X. maculatus genome with one Y-specific copy. Interestingly, it has specific copies on the W chromosome in the X. maculatus Usumacinta strain （sex determination with female het- erogamety）, which represent new markers for this type of sex chromosome in platyfish. This marker with W- and Y-specific copies suggests relationship between different types of gonosomes and allows comparing male and female heterogameties in the platyfish. Further molecular analysis of the DNA polymerase type B gene in X. maculatus will shed new light on the evolution of sex chromosomes in platyfish.

关键词： DNA polymerase type B chromosome marker Xiphophorus maculatus

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Protective effect of glycine on liver injury during liver transplantation

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Chinese Medical Journal 2010年第14期123卷 1931-1938页

作者： WANG Yao-sheng YAN Ye-hong ZOU Xun-feng Department of Cardiology the Second Affiliated Hospital of Nanchang University Nanchang Jiangxi 330006 China Department of Organ transplantation Center the First Affiliated Hospital of Nanchang University Nanchang Jiangxi 330006 China Institute fur physiologische chemie Universitatsklinikum Hufelandstr. 55 D-45122 Essen Germany

Multiple procedures of liver transplantation bring conditions producing cold ischemia-reperfusion （I/r） injury. During cold storage, the graft organ is subjected to cold ischemia, also known as hypoxia injury. After reperfusion, although hypoxic condition has been ameliorated, reoxygenation of the graft liver can produce not only reperfusion injury including generation of oxygen free radical, lipoperoxidation and calcium overload, but also aggravate the hypoxia damage,

关键词： glycine reperfusion injury liver transplantation Kupffer cells

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激光剥蚀-等离子体质谱技术及其在地球化学宇宙化学和环境研究中的应用(英文)

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岩矿测试 2009年第1期28卷 53-68页

作者： JOCHUM Klaus Peter STOLL Brigitte FrIEDrICH Jon M AMINI Marghaleray BECKEr Stefan DüCKING Marc EBEL Denton S ENZWEILEr Jacinta HU Ming-yue KUZMIN Dmitry MErTZ-KrAUS regina MüLLEr Werner E G rEGNErY Julia SOBOLEV Alexander WANG Xiao-hong ZHAN Xiu-chun Max Planck-lnstitut for chemie Mainz 55020 Germany Department of Earth and Planetary Sciences American Museum of Natural History New York 10024 USA Department of Chemistry Fordham University BronxNew York 10458 USA Saskatchewan Isotope Laboratory Department of Geological Sciences University of Saskatchewan Saskatoon S7N 5E2 Canada Bundeskriminalamt Wiesbaden 65173 Germany Instituto de Geoci6ncias University of Campinas CP 6152 Campinas CEP 13083-970 Brazil National research Center for Geoanalysis Beijing 100037 China Institut for Geowissenschaften Universitat Mainz Mainz 55099 Germany Institut for physiologische chemie Universitat Mainz Mainz 55099 Germany Institute of Geology and Mineralogy Siberian Branch of Russian Academy of Sciences Novosibirsk 630090 Russia V. I. Vernadsky Institute of Geochemistry and Analytical Chemistry Russian Academy of Sciences Moscow 119991 Russia

激光剥蚀-等离子体质谱(LA-ICPMS)已成为地球化学、宇宙化学和环境研究领域元素和同位素原位分析最重要的技术之一。文章介绍了多种类型的质谱仪及其使用的激光器。用途最广的LA-ICPMS仪器之一是单接收器扇形磁场质谱仪,配有Nd:YAG激光... 详细信息

激光剥蚀-等离子体质谱(LA-ICPMS)已成为地球化学、宇宙化学和环境研究领域元素和同位素原位分析最重要的技术之一。文章介绍了多种类型的质谱仪及其使用的激光器。用途最广的LA-ICPMS仪器之一是单接收器扇形磁场质谱仪,配有Nd:YAG激光剥蚀系统(激光波长分为193 nm和213 nm两种),MPI Mainz实验室使用的就是这套系统,文章对此作一详细介绍。文中阐述了数据优化技术及其多种校正过程;介绍LA-ICPMS在痕量元素和同位素分析领域的一些应用,包括参考物质的研制,Hawaiian玄武岩、Martian陨石、生物骨针和珊瑚虫中痕量元素分析及熔融包裹体和富钙-铝碳质球粒陨石中的铅和锶同位素测量。

关键词：激光剥蚀-等离子体质谱地质样品参考物质陨石生物样品痕量元素同位素比值

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