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Characteristics of low- frequency components of the near-bottom current in the Chinese Pioneer Area

Chinese Science Bulletin 2004年第11期49卷 1184-1189页

作者： LIANGChujin HOUYijun CHENQi DONGRuzhou DONGLixian instituteofoceanologychineseacademyofsciences Qingdao266071China TheSecondInstituteofOceanography StateofOceanAdministrationHangzhou310012China

Two deep-sea moorings were deployed respectively in the east area and the west area of Chinese Pioneer Area (CPA) in the tropic east Pacific to monitor the regional deep-sea dynamics below 600 meters above bottom (mab) from July 1997 to Oct. 1999. Results of statistics, spectral estimate and correlation analysis of the low-passed velocity data show that time scales of low-frequency components of the near-bottom currents are 25~120 days, in which 51-day period dominates the lower band of the frequency domain. Topographic features have obvious effect on low-frequency currents below 50 mab; modulations of the bot-tom-intensified sheared mean flow to the low-frequency currents are the dynamic mechanism of the frequency shift that occurs in both the east-area and the west-area.

关键词：低频组分 CPA 中国先进地区频率转移低频流深海动力学

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FATTY ACIDS PROFILE IN A HIGH CELL DENSITY CULTURE OF ARACHIDONIC ACID-RICH PARIETOCHLORIS INCISA (TREBOUXIOPHYCEAE,CHLOROPHYTA) EXPOSED TO HIGH PFD

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Chinese Journal of Oceanology and Limnology 2002年第2期20卷 149-156页

作者：刘建国 Zvi Cohen 等 ResearchandDevelopmentCenterofMarineBIotechnolgy InstituteofOceanologyChineseAcademyofSciencesQingdao266071China MicroalgalBiotechnology TheJacobBlausteinInstituteforDesertResea

The changes in arachidonic acid (AA) and fatty acids profiles along the growth curve of Parietochloris incisa, a coccoid snow green alga, were studied in a 2.8 cm light-path flat photobioreactor, exposed to strong photon flux density [PFD, 2400 μEmol/(m 2·s)]. Sixteen fatty acids were identified by gas chromatography showing that AA was the dominant fatty acid (33%-41%) followed by linoleic acid (17%-21%). AA content was closely investigated with respect to total fatty acids (TFA), ash free dry weight (AFDW) of cell mass as well as total culture content. These parameters were influenced significantly in a similar manner by culture growth phase, i.e., slightly decreasing in the lag period, gradually increasing in the logarithmic phase, becoming maximal at the early stationary phase, starting to decrease at the late stationary phase, sharply dropping at the decline phase. The increase in AA per culture volume during the logarithmic phase was not only associated with the increase in AFDW but also connected with a corresponding increase in AA/TFA, TFA/AFDW as well as AA/AFDW. The sharp decrease in AA content of the culture during the decline phase was mainly due to the decrease in AA/TFA, TFA/AFDW and AA/AFDW, although AFDW declined only a small extent. Maximal AA concentration, obtained at the early stationary phase, was 900 mg/L culture volume, and the average daily net increase of AA during 9 days logarithmic growth was 1.7 g/(m 2·day). Therefore, harvesting prior to the decline phase in a batch culture, or at steady state in continuous culture mode seems best for high AA production. The latter possibility was also further confirmed by continuous culture with 5 gradients of harvesting rate.

关键词： arachidonic acid Parietochloris incisa growth high cell density culture

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