检索结果-南通市图书馆

Spatio-Temporal Expression Pattern of Six Novel Candidate Genes in Ginsenoside Biosynthesis from Panax ginseng C. A. Meyer

引用

Journal of Integrative Plant Biology 2005年第5期47卷 622-631页

作者： Zhi-YongLUO Shui-PingLIU Xiang-HuiCHEN YingRUAN Jian-QingLUO BinWEN Chun-LinLIU Wei-XinHU instituteofmolecularbiology CollegeofBiologicalScienceandTechnologyCentralSouthUniversityChangsha410078China CropsGeneticEngineeringKeyLaboratoryofHunanProvince HunanAgriculturalUniversityChangsha410128China

Abstract: To explore the mode of the spatio-temporal expression of six newly discovered ginsenoside biosynthesis candidate gene transcripts, both Northern blotting and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) were used to elucidate the mRNA expression levels of the transcripts in various tissues and organs of Panax ginseng C. A. Meyer during different growth development stages. The six gene transcripts were all differentially expressed in cultured callus, root, stem, leaf, and seed. The mRNA expression levels were significantly higher in four-year-old roots than in one-year-old roots, and results of semi-quantitative RT-PCR assays were in accordance with those of Northern blotting analyses. The results strongly suggest that all six genes were differentially expressed at root-specific developmental stages. In particular, when a quiescent early stage culture suspension of P. ginseng cells was exposed to the ginsenoside biosynthesis-promoting elicitor Aspergillus niger polysaccharide, the GBR6 gene transcript response showed time-dependent increments and was parallel with ginsenoside productivity (P < 0.01). Overexpressionof the GBR6 gene is likely to play a critically important role in the biosynthesis of ginsenosides. The results of the present study provided a background for the further elucidation of the structure and physiological function of these six candidate genes.

关键词： Aspergillus niger polysaccharide elicitor GBR6 mRNA expression ginsenoside biosynthesis ginsenoside biosynthesis candidate genes mRNA differential expression Panax ginseng C. A. Meyer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Design and application of 60mer oligonucleotide microarray in SARS coronavirus detection

引用

Chinese Science Bulletin 2003年第12期48卷 1165-1169页

作者： SHIRong MAWenli WUQinghua ZHANGBao SONGYanbin GUOQiuye XIAOWeiwei WANGYan ZHENGWenling instituteofmolecularbiology FirstMilitaryMedicalUniversityGuangzhou510515China DepartmentofMedicalResearch GuangzhouLiuHuaQiaoHospitalGuangzhou510010China

The 60mer oligonucleotide microarray was designed and applied to detecting of SARS (severe acute respiratory syndrome) coronavirus. Thirty 60mer specific oligos were designed to cover the whole genome of the first submitted coronavirus strain, according to the sequence of TOR2 (GENEBANK Accession: AY274119). These primers were synthesized and printed into a microarray with 12×12 spots. RNAs were extracted from the throat swab and gargling fluid of SARS patients and reverse-transcripted into the double strand cDNAs. The cDNAs were prepared as restricted cDNA fragments by the restriction display (RD) technique and labeled by PCR with the Cy5-universal primer. The labeled samples were then applied to the oligo microarray for hybridization. The diagnostic capability of the microarray was evaluated after the washing and scanning steps. The scanning result showed that samples of SARS patients were hybridized with multiple SARS probes on the microarray, and there is no signal on the negative and blank controls. These results indicate that the genome of SARS coronavirus can be detected in parallel by the 60mer oligonucleotide microarray, which can improve the positive ratio of the diagnosis. The oligo microarray can also be used for monitoring the behavior of the virus genes in different stages of the disease status.

关键词：寡核苷酸微队列 SARS 冠状病毒检测 RD技术荧光标记分子杂交

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The effect of MAGE-A1 gene on NIH3T3 cells

引用

Journal of Nanjing Medical University 2005年第3期19卷 120-125页

作者： JingjunSun JinZhu ZhenningQiu YuhuaLi GuipengDing YiZhu ZhenqingFeng XiaohongGuan DepartmentofPathology NanjingMedicalUniversityNanjing210029China instituteofmolecularbiology NanjingMedicalUniversityNanjing210029China

Objective: Melanoma antigen genes(MAGE) genes have been found in many kinds of tumor tissue, but not in normal tissue except testis and placentas. The Ags encoded by MAGE genes therefore are strictly tumor-specific. The most current researches associated with these genes focus on the tumor vaccination using these Ags. Few reports are concerning these genes' functions. In this study, we investigated the role of MAGE-A1 gene on NIH3T3 cells after transferring with it. Methods: Clone the MAGE-A1 into the plasmids pEGFP-C3 and pcDNA3.1, then transfer the reconstructed plasmids and primary plasmids into the NIH3T3 cells using a new transfer reagent FuGENE 6. Selecting the positively transferred cells by G418. Identified by RT-PCR, Western blot, Immunocytochemistry, Laser Scanning Confocal Microscope and Fluoroscope. The cells mobile ability was measured with Millicell-PCF. The cell cycle and apoptosis were measured with Flow Cytometry. Results:The apoptosis rate of NIH3T3 cells that transferred with control plasmid pcDNA3.1 was 13.4% and the ratios that stay in S phase and G2-M phase were 5.68% and 1.04% respectively. The apoptosis rate of NIH3T3 cells that transferred with pcDNA3.1-A1 was 0.90% and the ratios that stayed in S phase and G2-M phase were 19.31% and 13.47% respectively. The apoptosis rate of the cells that transferred with control plasmid pEGFP-C3 was 1.87%, a little higher than 1.47% of those transferred with pEGFP-C3-A1. Conclusion:The MAGE-A1 gene may enhance the cell cycle, inhibit the apoptosis and raise the mobile (ability) of NIH3T3 cells.

关键词： melanoma antigen genes-A1 cell cycle apoptosis gene transfer mobile ability

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A Study on Antitoxic Role of Vesicular Monoamine Transporter 2 in Transgenic Chinese Hamster Overy Cells

引用

Journal of Nanjing Medical University 2003年第2期17卷 87-92页

作者：叶民丁新生董海蓉仇镇宁管晓虹 DepartmentofNeurology theFirstAffiliatedHospitalofNanjingMedicalUniversityNanjing210029 instituteofmolecularbiology NanjingMedicalUniversityNanjing210029P.R.China

Objective : To study the antitoxic role of vesicular monoamine transporter 2 (VMAT2) in transgenic Chinese Hamster ovary (CHO) cell. Methods :With the technology of trans-gene from PC 12 to CHO, MTT reduction assay was used to detect MPP+ toxic effect on wild type CHO (wtCHO) and transgenic CHO. Meanwhile, the role of reserpine was also observed in MPP+ toxic effects. Results :The sensitivity of transgenic CHO to MPP+ was much less than that of wtCHO with 0. 5 mmol/L MPP+. Transgenic CHO had the same sensitivity as wtCHO if rotenone was given. WtCHO, by given reserpine alone, didn't change its sensitivity to MPP+. Conclusions :VMAT2 has protective effect on transgenic CHO by transporting MPP+ to vesicles.

关键词： transgenic Chinese Hamster Ovary Vesicular Monoamine Transporter 2 antitoxic MPP+ reserpine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Construction and expression of yeast homologous recombination vector containing green fluoresent protein gene

引用

中国组织化学与细胞化学杂志 2004年第3期13卷 338-338页

作者： LianmeiHu YingjiePiao WenglingZheng CaiguHe WenliMa DongCui DepartmentofHistologyandEmbryology FirstUniformMedicalUniversityGuangzhou510515 DepartmentofExperimentalMedicine GuangzhouLiuHuaQiaoHospitalGuangzhou510010 instituteofmolecularbiology FirstUniformMedicalUniversityGuangzhou510515P.R.China

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Agrobacterium-mediated Transformation of Rice (Oryza sativa L.) with Atrazine Chlorohydrolase Gene (atzA)

引用

Agricultural Sciences in China 2005年第4期4卷 241-246页

作者： WANGSong-wen SHILi-li SUNZong-xiu CAIBao-li FUYa-ping WANGYang SIHua-min LIUXia ZHANGXin TianjinAgricultralCollege Tianjin300384P.R.China TianjinAgricultralCollege Tianjin300384P.R.China／／KeyLaboratoryforRiceBiologyMinistryofAgriculture／ChinaNationalRiceResearchInstituteHangzhou310006P.R.China KeyLaboratoryforRiceBiology MinistryofAgriculture~ChinaNationalRiceResearchInstituteHangzhou310006P.R.China instituteofmolecularbiology NankaiUniversityTianjin300071P.R.China KeyLaboratoryforMolecularPhytophathology MinistryofAgriculture／ChinaAgriculturalUniversityBeijing100094P.R.China

Atrazine chlorohydrolase gene (atzA) was cloned from Arthrobacter sp. AD1. A plant expression plasmid was constructed under the control of CaMV35s promoter and was used in rice transformation. The target gene was successfully introduced into mature embryos of a japonica rice cultivar Jindao 107 by Agrobacterium- mediated transformation and hundreds of transgenic plants were obtained. The exogenous atzA gene in the transgenic plants that expressed atrazine resistance was confirmed by Southern blot hybridization. The resistance experiments by spraying transgenic rice plants with 0.133% atrazine shown that most of the transgenic rice plants exhibited the resistance to herbicide atrazine. The segregation of exogenous atzA gene in T1 progeny corresponded to the Mendelian ratio.

关键词： Transgenic rice Arthrobacter sp. AD1 Atrazine chlorohydrolase gene

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：