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Characterization of RAPD Markers,and the RFLP Marker Linked to Powdery Mildew Resistance Gene Derived from Different Accessions of ***

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Agricultural Sciences in China 2005年第2期4卷 87-93页

作者： CHENXiao SHIAi-nong KONGFan-jing SLeath JPMurphy JIAXu InstituteofCropBreedingandCultivation ChineseAcademyofAgriculturalSciencesBeijing100081P.R.China NorthCarolinaStateUniversity RaleighNC27965USA instituteofgeneticsanddevelopmentalbiologythechineseacademyofsciences／thestatekeylaboratoryofplantcellandchromosomeengineering Beijing100101P.R.China

The analysis was carried out on performance of the resistance gene from Haynaldia villosa accession of the former Soviet Union to different isolates of Bluemerie graminis. Polymorphisms were revealed between 6D/6V substitution line Pm930640 and its pedigree parents using five RAPD markers of OPAN031700, OPAI01700, OPAL03750, OPAD07480 and OPAG15580 screened out from 120 random 10-mers primers. Three RAPD markers of OPAN03, OPAI01 and OPAL03 were linked with the resistance gene by analysis of F2 population of Chancellor×Pm930640. Analysis of 29 wheat lines including part of lines conferring the known genes from Pm1 to Pm20 respectively, lines conferring resistance gene from two H. villosa accessions and the related wheat parents, were analyzed and the results showed that these markers not only linked to the gene resistant to powdery mildew from H. villosa, but also detected different genetic backgrounds. OPAL03750 can be used as the marker to distinguish the different resistant lines from two H. villosa accessions because it was only observed in the materials from H. villosa of the former Soviet Union. RFLP analysis also showed the polymorphisms between two H. villosa accessions and their derived resistant lines.

关键词： RAPD RFLP Powdery mildew resistance gene Haynaldia villosa

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AtbHLH29 of Arabidopsis thaliana is a functional ortholog of tomato FER involved in controlling iron acquisition in strategy I plants

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Cell Research 2005年第8期15卷 613-621页

作者： You Xi YUAN1,2, Juan ZHANG1,2, Dao Wen WANG1, Hong Qing LING1,* 1The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing 100101, China 2Graduate School of the Chinese Academy of Sciences, Yuquan Road, Shijingshan District, Beijing 100039, China thestatekeylaboratoryofplantcellandchromosomeengineering InstituteofGeneticsandDevelopmentalBiologyChines

AtbHLH29 of Arabidopsis, encoding a bHLH protein, reveals a high similarity to the tomato FER which is proposed as a transcriptional regulator involved in controlling the iron deficiency responses and the iron uptake in tomato. For identification of its biological functions, AtbHLH29 was introduced into the genome of the tomato FER mutant T3238fer mediated by Agrobacterium tumefaciencs. Transgenic plants were regenerated and the stable integration of AtbHLH29 into their genomes was confirmed by Southern hybridization. Molecular analysis demonstrated that expression of the exogenous AtbHLH29 of Arabidopsis in roots of the FER mutant T3238fer enabled to complement the defect functions of FER. The transgenic plants regained the ability to activate the whole iron deficiency responses and showed normal growth as the wild type under iron-limiting stress. Our transformation data demonstrate that AtbHLH29 is a functional ortholog of the tomato FER and can completely replace FER in controlling the effective iron acquisition in tomato. Except of iron, FER protein was directly or indirectly involved in manganese homeostasis due to that loss functions of FER in T3238fer resulted in strong reduction of Mn content in leaves and the defect function on Mn accumulation in leaves was complemented by expression of AtbHLH29 in the transgenic plants. Identification of the similar biological functions of FER and AtbHLH29, which isolated from two systematically wide-diverged “strategy I” plants, suggests that FER might be a universal gene presented in all strategy I plants in controlling effective iron acquisition system in roots.

关键词： tomato AtbHLH29 iron uptake Arabidopsis FER plant nutrition.

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Expression of Rice Glutelin Gene GluA-2 in Wheat Endosperm

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Acta Botanica Sinica 2004年第10期46卷 1220-1225页

作者： CHENYu LIHui LIHong-Jie LIYi-Wen ZHUYin-Feng QULe-Qing JIAXu StateKeyLaboratoryofPlantCellandChromosomeEngineering InstituteofGeneticsandDevelopmentalBiologyTheChineseAcademyofSciencesBeijing100101China

Rice (Oryza sativa L.) glutelin accounts for about 80% of total seed storage protein. Glutelin can be easily digested by human and contains high concentrations of lysine and other essential amino acids. To improve the nutritional quality of wheat (Triticum aestivum L.), the cDNA sequence of rice glutelin gene GluA-2was introduced into common wheat through biolistic bombardment. Six hundreds of immature embryos from wheat cultivar Bobwhite (*** cv. Bobwhite) were bombarded. Four transgenic plants carrying GluA-2 gene were confirmed by PCR and Southern blotting analyses. Gene GluA-2 was translated in three of the transgenic plants and their progeny, as indicated by SDS-PAGE analyses. The expression of GluA-2 gene was not detected in one transgenic plant and the endogenous wheat high molecular weight glutenin subunits Bx7 and By9 contents decreased greatly in both To and T1 generations.

关键词：谷蛋白基因表达水稻胚乳小麦

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Molecular and functional characterization of sulfiredoxin homologs from higher plants

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Cell Research 2006年第3期16卷 287-296页

作者： Xian Peng Liu~(1,2) Xue Ying Liu~(1,2) Juan Zhang~(1,2) Zong Liang Xia~(1,2) Xin Liu~1 Huan Ju Qin~1 Dao Wen Wang~11 The State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Datun Road,Chaoyang District,Beijing 100101,China 2 Graduate University of Chinese Academy of Sciences,Yuquan Road,Shijingshan District,Beijing 100039,China thestatekeylaboratoryofplantcellandchromosomeengineering InstituteofGeneticsandDevelopmentalBiologyChineseAcademyofSciencesDatunRoadChaoyangDistrictBeijing100101China

By reducing cysteine-sulfinic acid in oxidized peroxiredoxin,sulfiredoxin (Srx) plays an important role in oxidationstress resistance in yeast and human ***,we report the first molecular and functional characterization of Srxhomolog from higher *** analysis revealed the presence of potential Srx encoding sequences in bothmonocot and dicot plant *** plant Srx proteins exhibited significant identities to their orthologs from yeastand human,and contained the conserved signature sequence and residues essential for ***,unlike yeastand human orthologs,plant Srxs were all predicted to possess chloroplast transit peptide in their primary *** proteins from Arabidopsis and rice (designated as AtSrx and OsSrx,respectively) complemented functional defi-ciency of Srx in the SRXl deletion yeast cells.A GFP fusion protein of AtSrx was targeted to chloroplast in Arabidopsismesophyll *** transcription occurred in both vegetative and reproductive organs,and the highest transcriptlevel was detected in *** oxidation stress,AtSrx transcript level was substantially increased,which paralleledwith enhanced transcription of 2-Cys peroxiredoxins that have been found essential in maintaining chloroplast *** addition to oxidation stress,osmotic/water deficit or cold treatments also raised AtSrx transcript ***-sistent with above findings,the knock-out mutant of AtSrx was significantly more susceptible to oxidation stress thanwild type Arabidopsis *** together,the results of this work indicate the existence of functional Srx homolog inhigher plants that is essential for plants to cope with oxidation stress.

关键词： Arabidopsis sulfiredoxin chloroplast reactive oxygen species stress

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