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Systemic immune responses to oral administration of recombinant attenuated Salmonella typhimurium expressing Helicobacter pylori urease in mice

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World Journal of Gastroenterology 2005年第14期11卷 2154-2156页

作者： Xiao-FengLiu Jia-LuHu Qi-ZhengQuan Zi-QinSun Yao-JunWang FengQi DepartmentofGastroenterology GeneralHospitalofJinanMilitaryCommandAreaJinan250031ShandongProvinceChina instituteofgastroenterology XijingHospitalFourthMilitaryMedicalUniversityXi'an710032ShaanxiProvinceChina

AIM:To evaluate whether attenuated Salmonella typhimurium producing Helicobacter pylori(H pylori) urease subunit B (UreB) could induce systemic immune responses against Hpylori infection. METHODS: Attenuated 5. typhimurium SL3261 was used as a live carrier of plasmid pTC01-UreB, which encodes recombinant H pylori UreB protein. Balb/c mice were given oral immunization with two doses of SL3261/pTC01-UreB at a 3-wk interval. Twelve weeks after oral immunization of mice, serum IgG antibodies were evaluated by ELISA assay. Gamma interferon (IFN-γ) and interleukin 10 (IL-10) in the supernatant of spleen cell culture were also assessed by ELISA. RESULTS: After oral immunization of mice, serum specific IgG antibodies against UreB in vaccine group were much higher than that in PBS and native Salmonella SL3261 control groups (A450, 0.373±0.100 vs 0.053±0.022, 0.142±0.039, respectively, P<0.01). Moreover, IFN-γ in vaccine group was on average 167.53±29.93 pg/mL, which showed a significant increase vs that of PBS control group (35.68±3.55 pg/mL, P<0.01). There was also a tremendous increase of IL-10 in vaccine group compared to PBS and SL3261 control groups (275.13±27.65 pg/mL vs 56.00±7.15 pg/mL, 68.02±15.03 pg/mL, respectively, P<0.01). In addition, no obvious side effects in mice and no change in gastric inflammation were observed. CONCLUSION: The multiple oral immunizations with the attenuated 5. typhimurium expressing Hpylori UreB could induce significant systemic immune responses, suggesting it may be used as oral vaccine against H pylori infection.

关键词： Helicobacter pylori Salmonella typhimurium Vaccine Systemic immune

Pleckstrin homology domain of G protein-coupled receptor kinase-2 binds to PKC and affects the activity of PKC kinase

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World Journal of Gastroenterology 2003年第4期9卷 800-803页

作者： Xing-LongYang Ya-LiZhang Zhuo-ShengLai Fei-YueXing Yu-HuLiu instituteofgastroenterology NanFangHospitalFirstMilitaryMedicalUniversity510515GuangzhouGuangdongProvinceChina Keylab.forshockandmicrocirculationofPLA DepartmentofPreclinicalMedicineFirstMilitaryMedicalUniversity510515GuangzhouGuangdongProvinceChina

AIM: To study the detail mechanism of interaction between PKC and GRK2 and the effect of GRK2 on activity of ***: The cDNA of pleckstrin homology (PH) domain located in GRK2 residue 548 to 660 was amplified by PCR with the mRNA of human GRK2 (β1-adrenergic receptor kinase) as template isolated from human fresh placenta,the expression vector pGEX-PH inserted with the aboved cDNA sequence for GRK2 PH domain protein and the expression vectors for GST (glutathion-s-transferase) -GRK2PH domain fusion protein, BTK (Bruton′s tyrosine kinase)PH domain and GST protein were constructed. The expression of GRK2 in culture mammalian cells (6 cell lines:PC-3,MDCK, SGC7901, Jurkat cell etc.) was determined by SDS-PAGE and Co-immunoprecipitation. The binding of GRK2PH domain, GST-GRK2 PH domain fusion protein and BTK PH domain to PKC in Vitro were detected by SDS-PAGE and Western blot, upon prolonged stimulation of epinephrine,the binding of GRK2 to PKC was also detected by western blot and ***: The binding of GRK2 PH domain to PKC in Vitro was confirmed by western blot, as were the binding upon prolonged stimulation of epinephrine and the binding of BTK PH domain to PKC. In the present study, GRK2 PH domain was associated with PKC and down-regulated PKC activity,but Btk PH domain up-regulated PKC activity as compared with GRK2 PH ***: GRK2 can bind with PKC and down-regulated PKC activity.

关键词： β1肾上腺受体激酶谷胱甘肽-S-转换酶蛋白激酶C G蛋白复合受体激酶-2 脱敏作用

Relationship between plasma D-dimer levels and clinicopathologic parameters in resectable colorectal cancer patients

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World Journal of Gastroenterology 2004年第6期10卷 922-923页

作者： GangXu Ya-LiZhang WenHuang instituteofgastroenterology FirstMilitaryMedicalUniversityGuangzhou510515GuangdongProvinceChina

AIM:To assess the clinical significance of the D-dimer levels and the relationship between plasma D-dimer levels and clinicopathologic parameters in operable colorectal cancer ***:The plasma levels of D-dimer were measured pre- and postoperatively in 35 patients with colorectal cancer, and 30 healthy subjects served as controls by the method of quantitative enzyme-linked immunosorbent assay (ELISA).RESULTS: The mean preoperative plasma levels of D-dimer in the patients with colorectal cancer (1.06±0.24mg/L) were significantly higher than those of controls (0.33±0.12mg/L,P<0.01). The D-dimer levels were remarkably elevated on the 1st day after operation (1.22±0.55mg/L,P<0.01).On the 3rd day the level of D-dimer began to stepwise descend and on the 14^th day nearly returned to control *** preoperative levels of D-dimer were significantly correlated with the lymph node metastasis and Dukes stage but had no association with tumor location and the degree of differentiation.A stepwise increase in the mean D-dimer levels was found with increase of the tumor ***:Hypercoagulation and higher fibrinolytic activities occur in patients with colorectal cancer. The operative trauma could enhance the fibrinolysis in the patients with colorectal *** measurement of preoperative D-dimer levels is considered to be useful for predicting lymph node metastasis and stage of colorectal cancer.

关键词：可切除结直肠癌 D-二聚体病理特点血清学检查酶联免疫吸附法

The Study of A Liposomal Tumor Vaccine Coentrapping β-Elemene and Soluble Antigens of Hepatocellular Carcinoma H22 Cells Modified by NDV

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胃肠病学 2000年第B8期5卷 135-136页

作者： DuanFang-ling GaoTian-hui instituteofgastroenterology TheSecondAfftliatedHospitalHenanMedicalUniversityZhengzhou450003China

Oral Immunization of Mice with Attenuated Salmonella Typhimurium Expressing Helicobacter pylori Urease B Subunits

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胃肠病学 2000年第B8期5卷 87-87页

作者： LiuXiao-feng HuJia-lu HuangJian-sheng instituteofgastroenterology XijingHospitalFourthMilitaryMedicalUniversityXi＇an710032China instituteofgastroenterology XijingHospitalFourthMilitaryMedicalUniversityXi＇an710032China

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胃肠病学 2000年第B8期5卷 145-145页

作者： LiuShi ZhouKai-fang HouXiao-hua YiChui-qiong instituteofgastroenterology UnionHospitalTongjiMedicalUniversityWuhan430022China