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Mechanisms of delayed re—epithelization in wounds combined with whole—body irradiation injury

中国临床康复 2002年第17期6卷 2652-2653,F003页

作者：屈纪富程天民等 instituteofcombinedinjuryofpla ThirdMilitaryMedicalUniversityChongqing400038China

Objective: To investigate the mechanisms of delayed re-epithelization in wounds combined with whole-body irradiation (WBI) injury. Methods: We observed re-epithelization in wounds by histological methods and transmission electron microscopy, detected the contents of proliferation cell nuclear antigen (PCNA) protein and mRNA using immunohistochemistry and in situ hybridization (ISH) methods. Results: Re-epithelization in wounds combined with WBI injury was significantly delayed as compared with simple incision injury, and the contents of PCNA protein and mRNA significantly decreased too. Conclusions: WBI injury has direct effect on wound epidermic cells, especially the suppress of epidermic basal cell proliferation, which may be an important reason why wound combined with WBI injury heals more slowly.

关键词：全身放射损伤合并症伤口愈合创伤表皮再上皮化

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Cloning and expression of mouse peroxiredoxin I in 1EC-6 Cells

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World Journal of Gastroenterology 2004年第14期10卷 2109-2112页

作者： BoZhang Yong-PingSu TaoWang Feng-ChaoWang Guo-PingAi HuiXu Jun-PingWang Yue-ShengHuang Jian-XinJiang instituteofcombinedinjuryofpla StateKeyLaboratoryofTraumaBurnsandCombinedInjuryThirdMilitaryMedicalUniversityChongqing400038China

AIM: To clone and express mouse peroxiredoxin Ⅰ in IEC-6 ***: Total RNAs were isolated from cultured IEC-6 cells, and the coding region of peroxiredoxin I was amplified by RT-PCR. After it was cloned into T-vector and sequenced,pSG5 was used to transiently express peroxiredoxin I in IEC-6 by liposome-mediated transfection, and the expression of peroxiredoxin I was evaluated by RT-PCRand Western blot. RESULTS: A DNA fragment about 750 bp was amplified from total RNAs of IEC-6 cells using specific primers of peroxiredoxin Ⅰ. The sequencing confirmed the coding region was successfully cloned into T-vector, which was completely coincident with the sequence in GeneBank. After the EcoRI-BamHI fragment of T-vector containing peroxiredoxin I was inserted into pSG5, the recombinant plasmid was transferred to IEC-6 cells. RT-PCR assay showed that a DNA fragment of 930 bp could be amplified,which indicated the transcription of pSG5-Prx. Western blot confirmed the expression of peroxiredoxin Ⅰ in IEC-6 ***: Mouse peroxiredoxin I can be successfully expressed in IEC-6 cells.

关键词：无性繁殖基因表达老鼠 peroxiredoxinⅠ IEC-6细胞重组器官

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