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Cloning of Salt Tolerance-Related cDNAs from the Mangrove Plant Sesuvium portulacastrum L.

Journal of Integrative Plant Biology 2006年第8期48卷 952-957页

作者： Hui-Cai Zeng Liu-Hong Deng Chun-Fa Zhang State Key Laboratory of tropical Crop biotechnology Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Science （ CATAS） Kaikou 571101 China Environmental and Plant Protection institute CATAS Danzhou 571737 China

In an attempt to isolate and identify the target genes relevant to salt tolerance in a mangrove plant （Sesuvium portulacastrum L.）, a subtracted cDNA library was constructed via suppressive subtractive hybridization （SSH）, in which the poly（A）＋RNA isolated from salt-tolerant S. portulacastrum leaves was used as a tester, whereas the driver was poly（A）＋RNA, derived from salt-sensitive S. portulacastrum leaves. Screening of this subtracted cDNA library revealed five clones, of which the expression levels in the salt-tolerant plant were markedly higher than those observed in the salt-sensitive plant, indicating that these candidate clones may be involved in salt-tolerance pathways. Among the clones isolated, P66, P175, and P233 are novel because no significant similarity was obtained upon alignment with the GenBank database. Clone P89 demonstrated high homology with NADPH of Arabidopsis thaliana, whereas clone P152 was highly homologous with the gene encoding late embryogenesis abundant （LEA） protein of A. thaliana. The full-length gene of clone P152, with a predicated 344 amino acid residues, was shown to bear LEA-2 domains, a signature motif for proteins that have been enriched under salty and drought conditions. It is thus implied that clone P152 would be a salt-tolerance gene of S. portulacastrum. In addition, we have also developed a strategy for the extraction of total RNA from mangrove plants.

关键词： mangrove salt tolerance-related cDNAs Sesuvium portulacastrum suppressive subtractive hybridization.

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Quantification of Photosynthetic Pigments in Neopyropia yezoensis Using Hyperspectral Imagery

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Plant Phenomics 2023年第1期5卷 60-72页

作者： Shuai Che Guoying Du Xuefeng Zhong Zhaolan Mo Zhendong Wang Yunxiang Mao Key Laboratory of Marine Genetics and Breeding(Ministry of Education) College of Marine Life SciencesOcean University of ChinaQingdao266003China Key Laboratory of Utilization and Conservation of tropical Marine Bioresource(Ministry of Education) College of Fisheries and Life ScienceHainan Tropical Ocean UniversitySanya572002China Yazhou Bay Innovation institute Hainan Tropical Ocean UniversitySanya572025China Laboratory for Marine Biology and biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao266073China

Phycobilisomes and chlorophyll-a(Chla)play important roles in the photosynthetic physiology of red macroalgae and serve as the primary light-harvesting antennae and reaction center for photosystem Ⅱ.Neopyropia is an economically important red macroalga widely cultivated in East Asian *** contents and ratios of 3 main phycobiliproteins and Chla are visible traits to evaluate its commercial *** traditional analytical methods used for measuring these components have several ***,a high-throughput,nondestructive,optical method based on hyperspectral imaging technology was developed for phenotyping the pigments phycoerythrin(PE),phycocyanin(PC),allophycocyanin(APC),and Chla in Neopyropia thalli in this *** average spectra from the region of interest were collected at wavelengths ranging from 400 to 1000 nm using a hyperspectral *** different preprocessing methods,2 machine learning methods,partial least squares regression(PLSR)and support vector machine regression(SVR),were performed to establish the best prediction models for PE,PC,APC,and Chla *** prediction results showed that the PLSR model performed the best for PE(R_(Test^(2))=0.96,MAPE=8.31%,RPD=5.21)and the SVR model performed the best for PC(R_(Test^(2))=0.94,MAPE=7.18%,RPD=4.16)and APC(R_(Test^(2))=0.84,MAPE=18.25%,RPD=2.53).Two models(PLSR and SVR)performed almost the same for Chla(PLSR:R_(Test^(2))=0.92,MAPE=12.77%,RPD=3.61;SVR:R_(Test^(2))=0.93,MAPE=13.51%,RPD=3.60).Further validation of the optimal models was performed using field-collected samples,and the result demonstrated satisfactory robustness and *** distribution of PE,PC,APC,and Chla contents within a thallus was visualized according to the optimal prediction *** results showed that hyperspectral imaging technology was effective for fast,accurate,and noninvasive phenotyping of the PE,PC,APC,and Chla contents of Neopyropia in *** could benefit the efficiency of macro

关键词： prediction breeding visible

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DPB3 and DPB4 proteins regulate Medicago flowering and leaf anthocyanin biosynthesis

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Journal of Genetics and Genomics 2023年第6期50卷 450-453页

作者： Yingying Meng Chongnan Wang Qiqi Li Wenkai Ji Jiangqi Wen Kirankumar S.Mysore Yanxi Pei Lifang Niu Hao Lin biotechnology Research institute Chinese Academy of Agricultural SciencesBeijing 100081China College of Life Science Shanxi UniversityTaiyuanShanxi 030006China Department of Plant and Soil Sciences Institute for Agricultural BiosciencesOklahoma State UniversityArdmoreOK 73401USA Department of Biochemistry and Molecular Biology Institute for Agricultural BiosciencesOklahoma State UniversityArdmoreOK 73401USA

As important subunits of the leading-strand DNA polymerase epsilon,chromatin remodeling,and histone acetylation complexes,the H2A/H2B-like histone-fold domain-containing proteins DNA PO-LYMERASE II SUBUNIT B3(DPB3)and DPB4 play key roles in nucleosome assembly and heterochromatin maintenance during DNA replication in yeast,Drosophila,and mammals(He et al.,2017;Bellelli et al.,2018;Yu et al.,2018;Casari et al.,2021).

关键词： mammals nucleosome complexes

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Increased light availability enhances tolerance against ocean acidification-related stress in the calcifying macroalga Halimedaopuntia

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Acta Oceanologica Sinica 2022年第12期41卷 123-132页

作者： Zhangliang Wei Yating Zhang Fangfang Yang Lijuan Long Key Laboratory of tropical Marine Bio-resources and Ecology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Key Laboratory of tropical Marine biotechnology of Hainan Province Sanya Institute of Ocean Eco-Environmental EngineeringSouth China Sea Institute of OceanologySanya 572000China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China University of Chinese Academy of Sciences Beijing 100049China

Although the adverse impacts of ocean acidification(OA)on marine calcifiers have been investigated extensively,the anti-stress capabilities regulated by increased light availability are ***,the interactive effects of three light levels(30μmol photons/(m^(2)·s),150μmol photons/(m^(2)·s),and 240μmol photons/(m^(2)·s)combined with two pCO_(2)concentrations(400 ppmv and 1400 ppmv)on the physiological acclimation of the calcifying macroalga Halimeda opuntia were investigated using a pCO_(2)-light coupling *** OA negatively influenced algal growth,calcification,photosynthesis,and other physiological performances in *** relative growth rate under elevated pCO_(2)conditions significantly declined by 13.14%−41.29%,whereas net calcification rates decreased by nearly three-fold under OA ***,increased light availability enhanced stress resistance through the accumulation of soluble organic molecules,especially soluble carbohydrate,soluble protein,and free amino acids,and in combination with metabolic enzyme-driven activities,OA stress was *** carotenoid content under low light conditions increased markedly,and the rapid light curve of the relative electron transport rate was enhanced significantly by increasing light intensities,indicating that this new organization of the photosynthetic machinery in *** accommodated light variations and elevated pCO_(2)***,the enhanced metabolic performance of the calcifying macroalga *** mitigated OA-related stress.

关键词： ocean acidification calcifying macroalga light availability soluble organic molecules enzyme-driven activities

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Chemical constituents of marine medicinal mangrove plant Sonneratia caseolaris

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Chinese Journal of Oceanology and Limnology 2009年第2期27卷 288-296页

作者：田敏卿戴好富李晓明王斌贵 Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of Sciences Graduate School of Chinese Academy of Sciences State Key Laboratory of tropical Crops biotechnology Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agriculture Sciences

Twenty-four compounds including eight steroids (1–8), nine triterpenoids (9–16, 24), three flavonoids (20–22), and four benzenecarboxylic derivatives (17–19, 23) were isolated and identified from stems and twigs of medicinal mangrove plant Sonneratia caseolaris. The structures of the isolated compounds were determined by extensive analysis of their spectroscopic data. Among these metabolites, compounds 1, 4–20 and 22–24 were isolated and identified for the first time from S. caseolaris. In the in vitro cytotoxic assay against SMMC-7721 human hepatoma cells, compound 21 (3′,4′,5,7-tetrahydroxyflavone) exhibited significant activity with IC50 2.8 μg/mL, while oleanolic acid (14), 3,3′-di-O-methyl ether ellagic acid (18), and 3,3′,4-O-tri-O-methyl ether ellagic acid (19) showed weak activity. None of these compounds displayed significant antibacterial activites.

关键词：红树植物海桑化学成分药用三萜类化合物人肝癌细胞海洋黄酮类化合物

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Complementation and Expression Analysis of SoRablA and SoRab2A in Sugarcane Demonstrates Their Functional Diversification

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Journal of Integrative Plant Biology 2006年第12期48卷 1450-1457页

作者： Jia-Ming Zhang Anne W Sylvester Ding-Qin Li Xue-Piao Sun State Key biotechnology Laboratory for tropical Crops Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China Department of Botany University of Wyoming Laramie Wyoming 82071 USA

Mammalian and plant Rabl and Rab2 are small GTPases that regulate vesicle trafficking in the endoplasmic reticulum （ER） to Golgi compartments. Little is known about their functional diversification or potential interaction. We cloned sugarcane （Saccharum officinarum L.） Rab1A and Rab2A genes and studied their functional differences by expression and complementation experiments. We found differential expression of the two genes during sugarcane leaf development： SoRab2A expression declined from the dividing base to the maturing tip of the growing leaves, whereas SoRab1A was constitutively expressed, suggesting that SoRab2A is required for cell division and expansion and SoRablA is required for cells at all developmental stages. We used a yeast temperature sensitive ypt1-A 136D mutant strain to further investigate these shared and unique functions. Yptl is a small GTPase that regulates vesicle transport in the same cellular location as Rabl and Rab2. Neither SoRab1A nor SoRab2A alone could restore the growth of the mutant at restrictive temperatures when SoRab1A and SoRab2A were transformed separately. However, SoRab1A transformants maintained normal morphology and viability at non-permissive temperature, and resumed growth when returned to permissive temperature, whereas SoRab2A transformants died at non-permissive temperature, suggesting that SoRablA function is required for a cell＇s viability. Mutant growth was fully restored when SoRab1A and SoRab2A were co-transformed, indicating that SoRablA and SoRab2A complement each other and they both are needed to restore the function of ypt1-A136D. These results demonstrate that SoRab1A and SoRab2A serve distinct but overlapping functions, mostly by regulating the transportation of different sets of proteins.

关键词： complementation co-transformation functional diversification Rab GTPase sugarcane temperature sensitivemutant

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BmCH25H,a vertebrate interferon-stimulated gene(ISG)homolog,inhibits BmNPV infection dependent on its hydroxylase activity in Bombyx mori

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Insect Science 2023年第2期30卷 321-337页

作者： Hongyun Wu Junming Xia Shigang Fei Yeyuan Wang Mengmeng Zhang Yiyao Guo Xian Li Luc Swevers Jingchen Sun Min Feng Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding College of Animal ScienceSouth China Agricultural UniversityGuangzhouChina Insect Molecular Genetics and biotechnology National Center for Scientific Research DemokritosInstitute of Biosciences and ApplicationsAthensGreece

Cholesterol-25-hydroxylase(CH25H)has been identified as an interferon-stimulated gene(ISG)in mammals that exerts its antiviral effects by catalyzing the conversion of cholesterol to 25-hydroxycholesterol(25HC).However,invertebrates lack an antiviral system homologous to vertebrate interferons(IFNs)because the genomes of invertebrates do not encode IFN-like ***,CH25H is present in insect genomes and it therefore deserves further study of whether and by which mechanism it could exert an antiviral effect in *** this study,the Bombyx mori CH25H(BmCH25H)gene,of which the encoded protein has high homology with other lepidopteran species,was identified and located on chromosome ***,we found that the expression of BmCH25H was significantly upregulated in *** nucleopolyhedrovirus(BmNPV)-infected BmN cells and silkworm(***)larvae at the early infection *** inhibitory effect of BmCH25H on BmNPV replication was further demonstrated to depend on its catalytic residues to convert cholesterol to *** importantly,we demonstrated that during BmNPV infection,BmCH25H expression was increased through the Janus kinase–signal transducer and activator of transcription(JAK–STAT)pathway,similar to the induction of ISGs following virus infection in *** is the first report that CH25H has antiviral effects in insects;the study also elucidates the regulation of its expression and its mechanism of action.

关键词： Bombyx mori nucleopolyhedrovirus Bombyx mori Cholesterol 25-hydroxylase Interferon-stimulated genes Signal transducer and activator of transcription

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Glacial expansion of cold-tolerant species in low latitudes: megafossil evidence and species distribution modelling

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National Science Review 2023年第4期10卷 13-15页

作者： Luliang Huang Shufeng Li Weiye Huang Helanlin Xiang Jianhua Jin Alexei A.Oskolski State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources School of Life Sciences/School of Ecology Sun Yat-sen University State Key Laboratory of Palaeobiology and Stratigraphy Nanjing Institute of Geology and Palaeontology Chinese Academy of Sciences CAS Key Laboratory of tropical Forest Ecology Xishuangbanna Tropical Botanical Garden Chinese Academy of Sciences Department of Botany and Plant biotechnology University of Johannesburg Botanical Museum Komarov Botanical Institute of the Russian Academy of Sciences

The Pleistocene was a time of repeated glaciations that severely affected the land biota over large areas of Eurasia and North America. Drastic climatic fluctuations during the Pleistocene drove largescale extinctions, migrations and diversification in plant and animal *** dramatic processes had a strong impact on the distribution of modern flora and fauna.

关键词： CLIMATE PLEISTOCENE DIVERSITY RECORD CHINA

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The piRNA pathway is required for nucleopolyhedrovirus replication in Lepidoptera

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Insect Science 2023年第5期30卷 1378-1392页

作者： Junming Xia Shigang Fei Hongyun Wu Yifan Yang Wensheng Yu Mengmeng Zhang Yiyao Guo Luc Swevers Jingchen Sun Min Feng Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding College of Animal ScienceSouth China Agricultural UniversityGuangzhouChina Insect Molecular Genetics and biotechnology National Center for Scientific Research DemokritosInstitute of Biosciences and ApplicationsAthensGreece

The Piwi-interacting RNA(piRNA)pathway has been shown to be involved in the antiviral defense against RNA viruses,especially in mosquitoes,but its universality has been ***,we used the Bombyx mori nucleopolyhedrovirus(BmNPV)-infected silkworm as a model to explore the effects of the key factors of piRNA pathway,BmAgo3 and Siwi,on replication of a large DNA virus(belonging to the family of Baculoviridae).We demonstrated that BmAgo3 and Siwi could promote the replication of BmNPV through both overexpression and knockdown experiments in BmN cell lines and silkworm *** addition,we also studied the effect of PIWI-class genes on Autographa californica nucleopolyhedrovirus(AcMNPV)replication in the Spodoptera frugiperda cell line *** knocking down the expression of PIWI-class genes in Sf9,we found that Piwi-like-1 and Piwi-like-2-3 could inhibit AcMNPV replication,while Piwi-like-4-5 promoted virus *** study provides compelling evidence that the piRNA pathway affects host infection by exogenous viruses in ***,our results reflect the diversity of the roles of PIWI-class genes in virus infection of the host across *** study is the first to explore the interaction of PIWI-class proteins with DNA viruses,providing new insights into the functional roles of the piRNA pathway.

关键词： BmAgo3 Bombyx mori nucleopolyhedrovirus Piwi-interacting RNA Siwi

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Microbiome Engineering:A Promising Approach to Improve Coral Health

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Engineering 2023年第9期28卷 105-116页

作者： Jie Li Qingsong Yang Junde Dong Michael Sweet Ying Zhang Cong Liu Yanying Zhang Xiaoyu Tang Wenqian Zhang Si Zhang CAS Key Laboratory of tropical Marine Bio-resources and Ecology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China tropical Marine Biological Research Station in Hainan Chinese Academy of Sciences&Key Laboratory of Tropical Marine Biotechnology of Hainan ProvinceSanya Institute of Ocean Eco-Environmental EngineeringSanya 572000China Aquatic Research Facility Environmental Sustainability Research CentreUniversity of DerbyDerby DE221GBUK School of Ocean Yantai UniversityYantai 264005China

The world’s coral reefs are threatened by the cumulative impacts of global climate change and local *** largely by a desire to understand the interactions between corals and their symbiotic microorganisms,and to use this knowledge to eventually improve coral health,interest in coral microbiology and the coral microbiome has increased in recent *** this review,we summarize the role of the coral microbiome in maintaining a healthy metaorganism by providing nutrients,support for growth and development,protection against pathogens,and mitigation of environmental *** explore the concept of coral microbiome engineering,that is,precise and controlled manipulation of the coral microbiome to aid and enhance coral resilience and tolerance in the changing *** coral microbiome engineering is clearly in its infancy,several recent breakthroughs indicate that such engineering is an effective tool for restoration and preservation of these valuable *** assist with identifying future research targets,we have reviewed the common principles of microbiome engineering and its applications in improving human health and agricultural productivity,drawing parallels to where coral microbiome engineering can advance in the not-too-distant ***,we end by discussing the challenges faced by researchers and practitioners in the application of microbiome engineering in coral reefs and provide recommendations for future work.

关键词： Coral reef restoration Coral holobiont Beneficial microorganisms for corals Bleaching Microbiome engineering

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