检索结果-南通市图书馆

Pyrotinib and chrysin synergistically potentiate autophagy in HER2-positive breast cancer

Signal Transduction and Targeted Therapy 2024年第1期9卷 295-307页

作者： Xiaoxiao Liu Xing Zhang Zhiying Shao Xiaorong Zhong Xin Ding Liang Wu Jie Chen Ping He Yan Cheng Kunrui Zhu Dan Zheng Jing Jing Ting Luo institute for Breast Health Medicine Cancer CenterBreast CenterWest China HospitalSichuan University610041 ChengduSichuanChina Department of Radiation Oncology Cancer CenterAffiliated Hospital of Xuzhou Medical UniversityJiangsu Center for the Collaboration and Innovation of Cancer BiotherapyCancer InstituteXuzhou Medical University221000 XuzhouChina Department of orthopedics Trauma and Reconstructive SurgeryUniversity Hospital RWTH AachenAachen 52074Germany Cancer institute Xuzhou Medical University221000 XuzhouJiangsuChina Division of Nephrology and Transplantation Department of Internal MedicineUniversity Medical Center Rotterdam Erasmus MCRotterdam 3015 GDThe Netherlands institute for Breast Health Medicine Department of General SurgeryBreast CenterWest China HospitalSichuan University610041 ChengduSichuanChina institute for Breast Health Medicine West China HospitalSichuan University610041 ChengduSichuanChina

Human epidermal growth factor receptor 2(HER2)-positive breast cancer(BC)has been the most challenging subtype of BC,consisting of 20%of BC with an apparent correlation with poor *** that pyrotinib,a new HER2 inhibitor,has led to dramatic improvements in prognosis,the efficacy of pyrotinib monotherapy remains largely restricted due to its acquired ***,identifying a new potential antitumor drug in combination with pyrotinib to amplify therapeutic efficacy is a pressing ***,we reported a novel combination of pyrotinib with chrysin and explored its antitumor efficacy and the underlying mechanism in HER2-positive *** determined that pyrotinib combined with chrysin yielded a potent synergistic effect to induce more evident cell cycle arrest,inhibit the proliferation of BT-474 and SK-BR-3 BC cells,and repress in vivo tumor growth in xenograft mice *** may be attributed to enhanced autophagy induced by endoplasmic reticulum ***,the combined treatment of pyrotinib and chrysin induced ubiquitination and glucose-6-phosphate dehydrogenase(G6PD)degradation by upregulating zinc finger and BTB/POZ domain-containing family protein 16(ZBTB16)in tumorigenesis of ***,we identified that miR-16-5p was a potential upstream regulator of ZBTB16,and it showed a significant inverse correlation with *** of miR-16-5p overexpression by restoring ZBTB16 significantly potentiated the overall antitumor efficacy of pyrotinib combined with chrysin against HER2-positive ***,these findings demonstrate that the combined treatment of pyrotinib and chrysin enhances autophagy in HER2-positive BC through an unrecognized miR-16-5p/ZBTB16/G6PD axis.

关键词： HER2 synergistic Together

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genetic Engineered Ultrasound-Triggered Injectable Hydrogels for Promoting Bone Reconstruction

引用

Research 2024年第2期2023卷 431-447页

作者： Zhenyu Zhao Huitong Ruan Aopan Chen Wei Xiong Mingzhu Zhang Ming Cai Wenguo Cui Department of Orthopaedics Shanghai Tenth People's HospitalSchool of MedicineTongji University No.301 Middle Yanchang RoadShanghai 200072China Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint DiseasesShanghai Institute of Traumatology and OrthopaedicsRuijin HospitalShanghai Jiao Tong University Schoolof Medicine197 Rujin 2nd RoadShanghai 200025China Department of Foot and Ankle Surgery Beijing Tongren HospitalCapital Medical University1 Dongjiao MinxiangBeijing 100730China

Genetic engineering technology can achieve specific gene therapy for a variety of diseases, but the current strategy still has some flaws, such as a complex system, single treatment, and large implantation trauma. Herein, the genetic engineering injectable hydrogels were constructed by ultrasonic technology for the first time to realize in vivo ultrasound-triggered in situ cross-linking and cell gene transfection, and finally complete in situ gene therapy to promote bone reconstruction. First, ultrasound-triggered calcium release was used to activate transglutaminase and catalyze the transamidation between fibrinogen. Simultaneously, liposome loaded with Zinc-finger E-box-binding homeobox 1 (ZEB1) gene plasmid (Lip-ZEB1) was combined to construct an ultrasound-triggered in situ cross-linked hydrogels that can deliver Lip-ZEB1. Second, ultrasound-triggered injectable hydrogel introduced ZEB1 gene plasmid into endothelial cell genome through Lip-ZEB1 sustained release, and then acted on the ZEB1/Notch signal pathway of cells, promoting angiogenesis and local bone reconstruction of osteoporosis through genetic engineering. Overall, this strategy provides an advanced gene delivery system through genetic engineered ultrasound-triggered injectable hydrogels.

关键词： sustained release linking

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The pathogenic mechanism of syndactyly type V identified in aHoxd13Q50R knock-in mice

引用

Bone Research 2024年第2期12卷 349-360页

作者： Han Wang Xiumin Chen Xiaolu Meng Yixuan Cao Shirui Han Keqiang Liu Ximeng Zhao Xiuli Zhao Xue Zhang McKusick-Zhang Center for Genetic Medicine State Key Laboratory of Complex Severe and Rare DiseasesDepartment of Medical GeneticsInstitute of Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical CollegeBeijing 100005China Department of orthopedics State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100730China

Syndactyly type V (SDTY5) is an autosomal dominant extremity malformation characterized by fusion of the fourth and fifthmetacarpals. In the previous publication, we first identified a heterozygous missense mutation Q50R in homeobox domain (HD) ofHOXD13 in a large Chinese family with SDTY5. In order to substantiate the pathogenicity of the variant and elucidate the underlyingpathogenic mechanism causing limb malformation, transcription-activator-like effector nucleases (TALEN) was employed togenerate a Hoxd13Q50R mutant mouse. The mutant mice exhibited obvious limb malformations including slight brachydactyly andpartial syndactyly between digits 2-4 in the heterozygotes, and severe syndactyly, brachydactyly and polydactyly in *** on BMP2 and SHH/GREM1/AER-FGF epithelial mesenchymal (e-m) feedback, a crucial signal pathway for limbdevelopment, we found the ectopically expressed Shh, Grem1 and Fgf8 and down-regulated Bmp2 in the embryonic limb bud atE10.5 to E12.5. A transcriptome sequencing analysis was conducted on limb buds (LBs) at E11.5, revealing 31 genes that exhibitednotable disparities in mRNA level between the Hoxd13Q50R homozygotes and the wild-type. These genes are known to be involvedin various processes such as limb development, cell proliferation, migration, and apoptosis. Our findings indicate that the ectopicexpression of Shh and Fgf8, in conjunction with the down-regulation of Bmp2, results in a failure of patterning along both theanterior-posterior and proximal-distal axes, as well as a decrease in interdigital programmed cell death (PCD). This cascadeultimately leads to the development of syndactyly and brachydactyly in heterozygous mice, and severe limb malformations inhomozygous mice. These findings suggest that abnormal expression of SHH, FGF8, and BMP2 induced by HOXD13Q50R may beresponsible for the manifestation of human SDTY5.

关键词： BMP2 mechanism finding

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Superenhancers activate the autophagy-related genes Beclin1 and LC3B to drive metastasis and drug resistance in osteosarcoma

引用

Frontiers of Medicine 2022年第6期16卷 883-895页

作者： Hongyi Wang Zhuochao Liu Jun Wang Fangqiong Hu Qi Zhou Li Wei Qiyuan Bao Jizhuang Wang Jing Liang Zhihong Liu Weibin Zhang Department of orthopedics Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai200025China Shanghai institute of traumatology and orthopedics Shanghai200025China

Metastasis and drug resistance are the leading causes of poor prognosis in patients with *** the relevant factors that drive metastasis and drug resistance is the key to improving the therapeutic outcome of ***,we reported that autophagy was highly activated in metastatic *** found increased autophagolysosomes in metastatic osteosarcoma cell lines by using electron microscopy,Western blot,and immunofluorescence *** further examined the expression of the autophagy-related genes Beclin1 and LC3B in 82 patients through immunohistochemistry and found that Beclin1 and LC3B were highly related to unfavorable prognosis of *** of Beclin1 and LC3B reduced invasion,metastasis,and proliferation in metastatic osteosarcoma *** vitro and in vivo studies also demonstrated that inhibiting by 3-MA inhibited cell growth and ***,we demonstrated that autophagy-related genes were activated by SEs and that the inhibition of SEs by JQ-1 decreased the metastasis of ***,our findings highlighted the association of autophagy with osteosarcoma progression and shed new light on autophagy-targeting therapy for osteosarcoma.

关键词： osteosarcoma autophagy metastasis drug resistance Beclin1 LC3B

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Acetabular cup version modelling and its clinical applying on plain radiograms

引用

World Journal of orthopedics 2017年第12期8卷 929-934页

作者： Anton Denisov Stanislav Bilyk Anton Kovalenko Orthopedic Department Vreden Russian Research Institute of Traumatology and Orthopedics

AIM To measure the sensitivity and specificity of the cup version assessment by using only anteroposterior hip and pelvis views, evaluate the incidence of inadequate cup version in patients with repeated dislocations after total hip arthroplasty(THA). METHODS Radiographic retrospective analysis of 2 groups of patients, with follow up of 6-60 mo, after undergoing primary THA. First group of 32 patients(20 female, 12 male) with unilateral THA(32 hips) required early revision arthroplasty for reasons of dislocation. The mean age and mode were 59(from 38 to 83) and 66 ages respectively. The average body mass index(BMI) was 24.2(from 17.7 to 36.3), mode 23.9. Second group was consisted of 164 patients(101 female, 63 male) without dislocations during the follow-up period(170 hips). Among them 6 patients required bilateral THA. The mean age was 60(from 38 to 84) and mode 59. BMI was 24.8(17.2-36.8), mode 25.2. Clinical significance of the cup anteversion sign was estimated with cross tabulation 2 × 2. RESULTS The value of the χ~2 yates was 10.668(P < 0.01).Sensitivity of SAI(sign of anteversion insufficiency) was 29%(95%CI: 9%-46%), and specificity was 92%(95%CI: 88%-96%). Relative risk of dislocation in patients with SAI was 3.4(95%CI: 1.8-6.3). CONCLUSION This method provides the surgeons with the ability to perform a reliable and simple qualitative assessment of the acetabular component version. It can be useful during patient examination with early loosening of the implant, dislocations, and impingement. Additionally, it can provide necessary information during planning of revision surgery, especially when considering question about cup replacement, although final assessment of the cup position should be done with a computed tomography scan.

关键词： Hip arthroplasty Acetabular component Retroversion Dislocation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ultrastable in-situ silver nanoparticle dressing for effective prevention and treatment of wound infection in emergency

引用

Engineered Regeneration 2024年第1期5卷 111-123页

作者： Wei Wang Ziwei Hu Wanying Mo Min Ouyang Shihao Lin Xiang Li Chengxinqiao Wang Feng Yu Yupeng Wang Dongfang Zhou Key Laboratory of Mental Health of the Ministry of Education NMPA Key Laboratory for Research and Evaluation of Drug Metabolism&Guangdong Provincial Key Laboratory of New Drug ScreeningSchool of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China institute of Otolaryngology Head and neck surgery Guangzhou Red Croee Hospital of Jinan UniversityGuangzhou 510282China c Department of Orthopaedics and traumatology Zhujiang HospitalSouthern Medical UniversityGuangzhou 510282China

The use of antibacterial dressings is crucial in the prevention and treatment of wound infection in emergency ***,the efficacy of dressings is compromised by long-term storage or exposure to harsh ***,an ultrastable in-situ silver nanoparticle dressing(AgSNP@CD)was prepared for effective prevention and treatment of wound infection in *** fabrication process of AgSNP@CD is simple,suitable for large-scale *** to the strong interaction between the in-situ synthesized AgNPs and the cotton fabric,AgSNP@CD owned remarkable stability,thus retaining its antimicrobial efficacy even after long-term storage(up to 2 years)and under extreme conditions(damp heat,low temperatures,low-oxygen,water immersion,acid-alkali erosion).Both in vitro and in vivo results demonstrated the extraordinary antibacterial efficacy and stability of AgSNP@CD,facilitating infection prevention and wound healing in extreme *** particular,AgSNP@CD exhibited a superior treatment effect on severe bacteria-infected trauma and can prevent the occur-rence of sepsis *** exceptional stability and antibacterial efficacy of AgSNP@CD under complex and extreme conditions make it a well-suited dressing strategy for the prevention and treatment of wound infection in emergency.

关键词： Bacteria-infected wounds Silver nanoparticle dressing Ultra stability Infection prevention In emergency

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A comparison of the effect of alendronate and You-Gui-Wan on osteoporosis in female rats with kidney-yang deficiency

引用

Traditional Medicine Research 2024年第6期9卷 31-41页

作者： Mei-Mei Wu Xing-Peng He Wan-Ting Wu Wen-Jie Lu Yan-Yang Mai Kun-Cai Xu Yao-Feng Zhi Hai-Xin Mo Jia-Di He Xin Zhang Peng-Fei Li Clinical Experimental Center Jiangmen Engineering Technology Research Center of Clinical Biobank and Translational ResearchJiangmen Central HospitalJiangmen 529030China Traumatic orthopedics Baiyun Hospital of The First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510470China Department of Intelligent Engineering Guiyang Institute of Information Science and TechnologyGuiyang 550025China

Background:In traditional Chinese medicine,You-Gui-Wan(YGW)is typically used to treat osteoporosis associated with kidney-yang ***,there have been few mechanistic studies on the effectiveness of kidney-yang deficiency-type osteoporosis with *** further clarify the role of YGW in the effect of osteoporosis with kidney-yang deficiency,the study analyzed the therapeutic advantages of YGW by comparing the therapeutic effects of YGW and alendronate(ALN)on osteoporosis with kidney-yang ***:SPF female SD rats were randomly divided into control,osteoporosis,osteoporosis with kidney-yang deficiency,osteoporosis with kidney-yang deficiency+YGW and osteoporosis with kidney-yang deficiency+ALN *** for the control group,osteoporosis was induced by the removal of bilateral *** 12 weeks,rats with osteoporosis in the kidney-yang deficiency group had kidney-yang deficiency syndrome triggered by hydrocortisone for 14 *** were treated with YGW or ALN for 12 *** weights of rats were ***-eosin staining staining was used to observe pathological changes in bone trabeculae,liver,spleen,and kidneys of *** of the growth plate cartilage of rats in different groups was observed by safranine-O *** expression of osteoclast key indices(ACP)and osteoblast key indices(ALP)in the bone tissue of rats in the different groups was observed by immunohistochemical *** expression of bone resorption-related indicators(TRAP and NXT-1),bone formation-related indicators(BALP,BGP,and P1NP),and major indicators of kidney-yang deficiency(ACTH,T3,T4,cAMP,and cGMP)were observed using an ELISA detection *** expression levels of the main indices of liver function(ALT and AST)were detected in different ***:The differences between the osteoporosis with kidney-yang deficiency group and osteoporosis group were that the weight of rats and the expression of ACTH,T3,T4,and cAMP decreased significantl

关键词： osteoporosis kidney-yang deficiency You-Gui-Wan alendronate

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Unlocking epigenetics for precision treatment of Ewing’s sarcoma

引用

Chinese Journal of Cancer Research 2024年第3期36卷 322-340页

作者： Zhehao Fan Shuangshuang Dong Ning Wang Muhammad Babar Khawar Jingcheng Wang Haibo Sun institute of Translational Medicine Medical CollegeYangzhou UniversityYangzhou 225001China Jiangsu Key Laboratory of Experimental&Translational Non-Coding RNA Research Yangzhou 225001China Department of Pathology Northern Jiangsu People’s Hospital Affiliated to Yangzhou University/Clinical Medical CollegeYangzhou UniversityYangzhou 225001China Department of orthopedics Northern Jiangsu People’s Hospital Affiliated to Yangzhou UniversityYangzhou 225001China

Ewing’s sarcoma(EWS)is a highly aggressive malignant bone tumor primarily affecting adolescents and young *** the efficacy of chemoradiotherapy in some cases,the cure rate for patients with metastatic and recurrent disease remains ***,there is an urgent need for innovative therapeutic approaches to address the challenges associated with EWS *** regulation,a crucial factor in physiological processes,plays a significant role in controlling cell proliferation,maintaining gene integrity,and regulating *** studies highlight the importance of abnormal epigenetic regulation in the initiation and progression of EWS.A comprehensive understanding of the intricate interactions between EWS and aberrant epigenetic regulation is essential for advancing clinical drug *** review aims to provide a comprehensive overview of both epigenetic targets implicated in EWS,integrating various therapeutic modalities to offer innovative perspectives for the clinical diagnosis and treatment of EWS.

关键词： Ewing’s sarcoma epigenetic targets drug resistance drug development

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

CLINICAL STUDY ON MANIPULATIVE TREATMENT OF DERANGEMENT OF THE ATLANTOAXIAL JOINT

引用

Journal of Traditional Chinese Medicine 1999年第4期19卷 273-278页

作者：周卫蒋位庄李星章永东张霁吴子明 institute of orthopedics and traumatology China Academy of Traditional Chinese MedicineBeijing 100700China

The derangement of the atlantoaxial joint is one of main cervical sources of dizziness and headache, which were based on the observation on the anatomy of the upper cervical vertebrae, analysis of X-ray film of the atlantoaxial joint, and the manipulative treatment in 35 patients with cervical spondylosis. The clinical diagnosis of derangement consists of: dizziness, headache, prominence and tenderness on one side of the affected vertebra, deviation of the dens for 1 mm-4 mm on the open-mouth X-ray film, abnormal movement of the atlantoaxial joint on head-rotated open-mouth X-ray film. An accurate and delicate adjustment is the most effective treatment.

关键词： Atlanto-Axial Joint Cervical Vertebrae Manipulation, Spinal Adult Female Follow-Up Studies Humans Male Massage Middle Aged Spinal Diseases

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A pathological joint-liver axis mediated by matrikine-activated CD4^(+)T cells

引用

Signal Transduction and Targeted Therapy 2024年第6期9卷 2679-2693页

作者： Junzhi Yi Hui Zhang Fangyuan Bao Zhichu Chen Yuliang Zhong Tianning Ye Xuri Chen Jingyi Qian Mengya Tian Min Zhu Zhi Peng Zongyou Pan Jianyou Li Zihao Hu Weiliang Shen Jiaqi Xu Xianzhu Zhang Youzhi Cai Mengjie Wu Hua Liu Jing Zhou Hongwei Ouyang Department of Sports Medicine of the Second Affiliated Hospital and Liangzhu LaboratoryZhejiang University School of MedicineHangzhouChina Dr.Li Dak Sum&Yip Yio Chin Center for Stem Cells and Regenerative Medicine Zhejiang University School of MedicineHangzhouChina Stomatology Hospital School of StomatologyZhejiang University School of MedicineZhejiang Provincial Clinical Research Center for Oral DiseasesHangzhouChina The MOE Key Laboratory of Biosystems Homeostasis&Protection The Key Laboratory of Cancer Molecular Cell Biology of Zhejiang ProvinceLife Sciences InstituteZhejiang UniversityHangzhouChina Department of orthopedics Huzhou Central HospitalAffiliated Huzhou HospitalZhejiang University School of MedicineHuzhouChina China Orthopedic Regenerative Medicine Group(CORMed) HangzhouChina Department of orthopedics The First Affiliated HospitalZhejiang University School of MedicineHangzhouChina

The knee joint has long been considered a closed *** pathological effects of joint diseases on distant organs have not been ***,our clinical data showed that post-traumatic joint damage,combined with joint bleeding(hemarthrosis),exhibits a worse liver function compared with healthy *** mouse model,hemarthrosis induces both cartilage degeneration and remote liver ***,we found that hemarthrosis induces the upregulation in ratio and differentiation towards Th17 cells of CD4^(+)T cells in peripheral blood and *** of CD4^(+)T cells reverses hemarthrosis-induced liver *** of cartilage matrix induced by hemarthrosis upregulates serological type Ⅱ collagen(COL Ⅱ),which activates CD4^(+)T *** application of a COL Ⅱ antibody blocks the ***,bulk RNAseq and single-cell qPCR analysis revealed that the cartilage Akt pathway is inhibited by blood ***-articular application of Akt activator blocks the cartilage degeneration and thus protects against the liver impairment in mouse and pig *** together,our study revealed a pathological joint-liver axis mediated by matrikine-activated CD4^(+)T cells,which refreshes the organ-crosstalk axis and provides a new treatment target for hemarthrosis-related disease.

关键词： degeneration damage inhibited

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：