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Meta-QTL analysis for mining of candidate genes and constitutive gene network development for fungal disease resistance in maize(Zea mays L.)

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The Crop Journal 2023年第2期11卷 511-522页

作者： Mamta Gupta Mukesh Choudhary Alla Singh Seema Sheoran Deepak Singla Sujay Rakshit ICAR-Indian institute of Maize Research LudhianaPunjab 141004India School of Agriculture and Environment The University of Western AustraliaPerthWA 6009Australia The UWA institute of Agriculture The University of Western AustraliaPerthWA 6009Australia School of Agricultural Biotechnology Punjab Agricultural UniversityLudhianaPunjab 141004India

The development of resistant maize cultivars is the most effective and sustainable approach to combat fungal *** the last three decades,many quantitative trait loci(QTL)mapping studies reported numerous QTL for fungal disease resistance(FDR)in ***,different genetic backgrounds of germplasm and differing QTL analysis algorithms limit the use of identified QTL for comparative *** meta-QTL(MQTL)analysis is the meta-analysis of multiple QTL experiments,which entails broader allelic coverage and helps in the combined analysis of diverse QTL mapping studies revealing common genomic regions for target *** the present study,128(33.59%)out of 381 reported QTL(from 82 studies)for FDR could be projected on the maize genome through MQTL *** revealed 38 MQTL for FDR(12 diseases)on all chromosomes except chromosome *** MQTL namely 1_4,2_4,3_2,3_4,and 5_4 were linked with multiple *** of 1910 candidate genes were identified for all the MQTL regions,with protein kinase gene families,TFs,pathogenesis-related,and disease-responsive proteins directly or indirectly associated with *** comparison of physical positions of marker-traits association(MTAs)from genome-wide association studies with genes underlying MQTL interval verified the presence of QTL/candidate genes for particular *** linked markers to MQTL and putative candidate genes underlying identified MQTL can be further validated in the germplasm through marker screening and expression *** study also attempted to unravel the underlying mechanism for FDR resistance by analyzing the constitutive gene network,which will be a useful resource to understand the molecular mechanism of defense-response of a particular disease and multiple FDR in maize.

关键词： Meta-QTL Maize genome Fungal disease resistance Candidate gene Constitutive genes Gene network

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Capture and fission analysis of various superheavy isotopes with ACN=258-280

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Communications in Theoretical Physics 2020年第2期72卷 65-74页

作者： Gurjit Kaur Manoj K Sharma School of Physics and Materials Science Thapar Institute of Engineering and TechnologyPatiala—147004PunjabIndia

The capture and fission analysis of heavy ion induced fusion reactions leading to the formation of Z=107-111 superheavy nuclei has been carried *** have been made to analyze the synthesis traits,such as excitation functions,formation probabilities,barrier characteristics *** l-summed Wong model provides a decent description of available data on capture(σCap)and fusion-fission(σff)cross-sections and hence is exploited to make relevant predictions for future *** capture and fusion-fission excitation functions are predicted for the least explored region of superheavy nuclei(SHN)i.e.Z=*** role of massasymmetry(η),Coulomb factor(ZPZT)deformation and orientations,Businari-Gallone massasymmetry(αBG)fission barrier(Bf)etc is duly *** present study concludes that the mass-asymmetric reactions involving 24Mg,30Si,and 36S projectiles are preferred for the synthesis of unknown isotopes of Z=***,the doubly magic 48Ca-projectile also provides a competing alternative to produce neutron-rich isotopes of the abovementioned SHN.

关键词： Capture and fission cross-section compound nucleus formation probability quasi-fission

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Identification of novel catalytic features of endo-β-1,4-glucanase produced by mulberry longicorn beetle Apriona germari

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第10期8卷 765-770页

作者： SAMI Amtul Jamil HAIDER Mohammed Kamran institute of Biochemistry and Biotechnology University of the Punjab Lahore 54594 Pakistan

Mulberry longicorn beetle, Apriona germari, has been reported to produce two endo-β-1,4-glucanases or AgEGases (accession Nos. Q6SS52 and Q5XQD1). AgEGase sequence contains catalytic motif (amino acid residues 37~48), which is the characteristic of family Glycohydrolase 45 and is identified as the substrate binding site. The application of bioinformatics ap-proaches includes sequence analysis, structural modeling and inhibitor docking to relate the structure and function of AgEGases. We have dissected the sequence and structure of AgEGase catalytic motif and compared it with crystal structure of Humicola insolens endoglucanases V. The results show an involvement of sulfur containing amino acid residues in the active site of the enzyme. Cys residues and position of disulfide bonds are highly conserved between the two structures of endoglucanases of A. germari. Surface calculation of AgEGase structure in the absence of Cys residues reveals greater accessibility of the catalytic site to the substrate involving Asp42, a highly conserved residue. For the inhibition study, tannin-based structure was docked into the catalytic site of AgEGase using ArgusLab 4.0 and it resulted in a stable complex formation. It is suggested that the inhibition could occur through formation of a stable transition state analog-enzyme complex with the tannin-based inhibitor, as observed with other insect cellulases in our laboratory.

关键词： Cellulases AgEGase Apriona germari Inhibition Anthocyanidins

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Use of waste plastic oil as a fuel in reactivity-controlled compression ignition engines:a bibliometric investigation from 2017-23

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Clean Energy 2024年第3期8卷 206-222页

作者： Sumit Kanchan Sarbjot Singh Sandhu Department of Mechanical Engineering Dr B R Ambedkar National Institute of TechnologyJalandharPunjabIndia School of Mechanical Engineering Lovely Professional UniversityJalandharPunjabIndia

Waste plastic oil,generated through the pyrolysis of plastic without oxygen,known as plastic-to-fuel conversion,stands as a promising alternative *** research employs bibliometric analysis,a quantitative method,to comprehensively assess publications in the domain of waste plastic oil integration within reactivity-controlled compression ignition *** Scopus as the primary repository post-2017,the study categorizes information into pivotal aspects,including influential journals,countries,authors,affiliations,document types,thematic areas,sponsors and *** analysis reveals significant contributions from diverse nations,prominently India,China,Turkey and *** is the prevalence of Energy,Engineering and Chemical Engineering as thematic focal points,with the journal Fuel leading the count followed by the journal Energy emerging as a prominent *** contributions are prolific,led by Gandhi institute of Technology and Management University and Vellore institute of *** authors‘Gugulothu S.K’and‘He Z’play a pivotal role in shaping the *** National Natural Science Foundation of China stands out as a primary *** bibliometric analysis provides a structured understanding of waste plastic oil integration in reactivity-controlled compression ignition engines,laying the groundwork for future research *** study identifies potential research platforms beyond Scopus,including Web of Science,CrossRef,Science Direct and Google Scholar,as valuable repositories for further ***,after careful observation of the research articles published by the top 10 authors as found in the study,it was found that the authors share a few common characteristics that helped them in advancing the understanding and application of waste plastic oil in reactivity-controlled compression ignition *** are also summarized in the present study with supporting data.

关键词： bibliometric analysis plastic-to-fuel waste plastic oil RCCI engine Scopus database

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Surface Modification Aspects for Improving Biomedical Properties in Implants:A Review

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Acta Metallurgica Sinica(English Letters) 2024年第2期37卷 213-241页

作者： J.Sharath Kumar Rakesh Kumar Rajeev Verma Industrial and Production Department Dr B R Ambedkar National Institute of Technology JalandharJalandharPunjab144008India

The primary focus during surgery is to ensure successful implantation by achieving long-term and stable fixation of *** surgery is now more focused on the development of novel biomaterials intended to improve implant *** obtain a better understanding of metal implants,this article investigated the causes of material failures in certain cases and analysed a few case *** interaction between the implant and bone tissue was a crucial aspect of successful implantation,and this study explored the factors influencing this interaction as well as ways to improve *** modern approaches used for modifying implant surfaces were systematically illustrated and briefly *** spray coatings were often favoured because of their wide range of coating materials,but other substantial surface modifications(such as friction stir processing and laser surface texturing)were also used for a selection of ***,implant surfaces with desirable features,such as biocompatibility,antibacterial properties,corrosion resistance,and wear resistance,were essential for optimising implant *** systematic review's main aim is to provide exhaustive reference information and a broad overview to advance the production and design of orthopaedic implants.

关键词： Biomaterials Surface treatments Thermal spray coatings Antibacterial Biocompatibility Corrosion resistance Wear resistance

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Clean label extraction of bioactive compounds from food waste through microwave-assisted extraction technique-A review

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Food Bioscience 2022年第2期46卷 1020-1028页

作者： Tayyaba Alvi Zarnab Asif Muhammad Kashif Iqbal Khan National institute of Food Science and Technology University of AgricultureFaisalabad38000PunjabPakistan Department of Food Engineering University of AgricultureFaisalabad38000PunjabPakistan

Clean label and green extraction have attained popularity due to elimination of solvent use and reduction in energy *** this regard,microwave assisted extraction have emerged as a novel process for extraction of bioactive *** process observed to consume less time and energy but have higher extraction ***,the functionality of bioactive compounds has not degraded in this ***,this article explores the potential of microwave-based extraction as a green and ecofriendly ***,it has been coupled with conventional technique(*** distillation,steam diffusion,hydrodistillation,air hydrodistillation,and hydro-diffusion and gravity)to ease the process and increase the *** data highlights the importance of various hybrid techniques(microwave+conventional ones)for the extraction of bioactive compounds from fruit&vegetable waste.

关键词： Ecofriendly Green extraction Sustainable Solvent free extraction Fruit waste

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Medical Data Clustering and Classification Using TLBO and Machine Learning Algorithms

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Computers, Materials & Continua 2022年第3期70卷 4523-4543页

作者： Ashutosh Kumar Dubey Umesh Gupta Sonal Jain Chitkara University institute of Engineering and Technology Chitkara UniversityPunjabIndia institute of Engineering and Technology JK Lakshmipat UniversityJaipurIndia

This study aims to empirically analyze teaching-learning-based optimization(TLBO)and machine learning algorithms using k-means and fuzzy c-means(FCM)algorithms for their individual performance evaluation in terms of clustering and *** the first phase,the clustering(k-means and FCM)algorithms were employed independently and the clustering accuracy was evaluated using different *** the second phase,the non-clustered data obtained from the first phase were preprocessed with *** was performed using k-means(TLBO-KM)and FCM(TLBO-FCM)(TLBO-KM/FCM)*** objective function was determined by considering both minimization and maximization ***-clustered data obtained from the first phase were further utilized and fed as input for threshold *** benchmark datasets were considered from theUniversity of California,Irvine(UCI)Machine Learning Repository for comparative study and *** are breast cancer Wisconsin(BCW),Pima Indians Diabetes,Heart-Statlog,Hepatitis,and Cleveland Heart Disease *** combined average accuracy obtained collectively is approximately 99.4%in case of TLBO-KM and 98.6%in case of *** approach is also capable of finding the dominating *** findings indicate that TLBO-KM/FCM,considering different computational measures,perform well on the non-clustered data where k-means and FCM,if employed independently,fail to provide significant *** different feature sets,the TLBO-KM/FCM and SVM(GS)clearly outperformed all other classifiers in terms of sensitivity,specificity and ***/FCM attained the highest average sensitivity(98.7%),highest average specificity(98.4%)and highest average accuracy(99.4%)for 10-fold cross validation with different test data.

关键词： K-means FCM TLBO TLBO-KM TLBO-FCM TLBO-KM/FCM machine learning algorithms

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Optimizing mechanical property and cytocompatibility of the biodegradable Mg-Zn-Y-Nd alloy by hot extrusion and heat treatment

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Journal of Materials Science & Technology 2019年第1期35卷 6-18页

作者： Yiyuan Kang Beining Du Yueming Li Baojie Wang Liyuan Sheng Longquan Shao Yufeng Zheng Tingfei Xi Shenzhen institute Peking University Nanfang Hospital Southern Medical University institute of Metal Research Chinese Academy of Sciences

The Mg-Zn-Y-Nd alloy is a new type of degradable material for biomedical application. In the present study, Mg-6Zn-1.2Y-0.8Nd alloy was fabricated, and then extrusion and heat treatment were conducted to optimize its mechanical properties and cytocompatibility. The microstructure observation, mechanical property, degradation behavior and cytocompatibility tests were conducted on the Mg-Zn-Y-Nd alloy with three different states: as-cast(alloy C), as-extruded(alloy E) and extruded + heat treated(alloy EH).The results show that alloy C consists of coarse grains and continuous secondary phases. The extrusion process has caused incomplete recrystallization, and results in a mixed grain structure of elongated grains and small equiaxed grains(alloy E). The heat treatment process has promoted the recrystallization and homogenized the grain structure(alloy EH). Both the strength and ductility of the alloy has been improved by extrusion, but the following heat treatment has decreased the strength and increased the *** degradation behavior of the alloy C and E alloys does not show much difference, but improves slightly in alloy EH, because the heat treatment has homogenized the microstructure and released the residual stress in the alloy. The directly and indirectly cell viability tests indicate that alloy EH exhibits the best cytocompatibility, which should be ascribed to its relative uniform degradation and low ion releasing rate. In summary, the combination of hot extrusion and heat treatment could optimize the mechanical property and cytocompatibility of the Mg-Zn-Y-Nd alloy together, which is beneficial for the future application of the alloy.

关键词： Mg-Zn-Y-Nd alloy Microstructure Mechanical property Biodegradable behavior Biocompatibility

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State of the art in finite element approaches for milling process:a review

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Advances in Manufacturing 2023年第4期11卷 708-751页

作者： Shailendra Chauhan Rajeev Trehan Ravi Pratap Singh Department of Industrial and Production Engineering Dr.B R Ambedkar National Institute of TechnologyJalandharPunjabIndia

Over a century,metal cutting has been observed as a vital process in the domain of *** the numerous available metal-cutting processes,milling has been considered as one of the most employable processes to machine a variety of engineering materials *** the milling process,material removal occurs when the workpiece is fed against a rotating tool with multiple cutting *** order to maximize the profitability of metal cutting operations,it is essential that the various input and output variable relationships are analyzed and *** experimental method of studying milling processes is costly and time demanding,particularly when a large variety of elements such as cutting tool shape,materials,cutting conditions,and so on,are *** to these issues,other alternatives emerged in the form of mathematical simulations that employ numerical *** finite element approaches have well-proven to be the most practical and commonly utilized numerical *** finite element model(FEM)can be used to determine the various physical interactions occurring during the machining process along with the prediction of various milling characteristics,such as cutting forces,cutting temperature,stresses,etc.,with the help of milling *** the present article,various research studies in the broad milling process domain practiced with numerous finite element approaches have been critically reviewed and *** further highlights the several experimental and finite element approaches-based research studies that attempted to analyze and optimize the overall performance of the different milling *** recent years,various investigators have explored numerous ways to enhance milling performance by probing the different factors that influence the quality *** of the studies have also been found to be focused on the economic impacts of milling and various process inputs that affect milling ***,various milli

关键词： Milling Finite element modeling(FEM) Micro milling Turn-milling

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Sequential decay analysis of^(235)U(n^(th),f)reaction using fragmentation approach

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Chinese Physics C 2024年第6期48卷 145-154页

作者： Nitin Sharma Ashutosh Kaushik Manoj K.Sharma School of Physics and Materials Science Thapar Institute of Engineering&TechnologyPatiala-147004PunjabIndia

Numerous experimental and theoretical observations have concluded that the probability of the three fragment emission(ternary fission)or binary fission increases when one proceeds towards the heavy mass region of nuclear periodic *** factors affect fragment emission,such as the shell effect,deformation,orientation,and fissility *** and ternary fissions are observed for both ground and excited states of the *** collinear cluster tripartition(CCT)channel of the^(235)U(n^(th),f)reaction is studied,and we observe that the CCT may be a sequential or simultaneous emission *** date,different approaches have been introduced to study the CCT process as a simultaneous or sequential process,but the decay dynamics of these modes have not been not fully *** the three fragments of the sequential process and exploring their related dynamics using an excitation energy dependent approach would be of further ***,in this study,we investigate the sequential decay mechanism of the^(235)U(n^(th),f)reaction using quantum mechanical fragmentation theory(QMFT).The decay mechanism is considered in two steps,where initially,the nucleus splits into an asymmetric *** the second step,the heavy fragment obtained in the first step divides into two *** I analysis is conducted by calculating the fragmentation potential and preformation probability for the spherical and deformed choices of the decaying *** most probable fragment combination of stage I are identified with respect to the dips in the fragmentation structure and the corresponding maxima of the preformation probability(P0).The light fragments of the identified decay channels(obtained in step I)agree closely with the experimentally observed *** excitation energy of the decay channel is calculated using an iteration *** excitation energy is shared using an excitation energy dependent level density *** obtained excitation

关键词： fission cluster radioactivity ternary fission spontaneous fission cluster model total kinetic energy preformation probability

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