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Food Bioscience 2022年第2期46卷 658-669页

作者： Qian Ge Chunfeng Guo Yue Yan Xiangyu Sun Tingting Ma Jing Zhang Caihong Li Chunlin Gou Tianli Yue Yahong Yuan College of Food Science and Engineering Northwest A&F UniversityYangling712100China institute of quality standard and testing technology for agro-products of ningxia Yinchuan750002China Laboratory of quality&Safety Risk Assessment for agro-products(Yangling) Ministry of AgricultureYangling712100China

To acquire candidate non-Saccharomyces strains for preparation of high-quality Vidal icewine,11 representative indigenous non-Saccharomyces strains were selected for analyzing their production of organic acids,phenolic compounds,aroma-active compounds and evaluating their sensory profile in Vidal *** results showed that the contents of organic acids in the wines were controlled and balanced well by the non-Saccharomyces strains as well as polyphenol concentrations were well improved during the *** strains differed from each other in their ability to produce aroma compounds,causing diversity and complexity of aroma characteristics in *** by Saccharomycopsis crataegensis YC30(Sc)and Hanseniaspora uvarum QTX22(Hu)could enhance the sweet and floral notes in Vidal icewine,especially Sc,which has never been reported *** can not only enrich the aroma categories,but also increase the content ofδ-decalactone,γ-decalactone,ethyl isobutyrate,ethyl butyrate,phenethyl acetic acid,*** evaluation and optimization of non-Saccharomyces yeast strains could provide theoretical basis for the improvement of ice wine quality,and supply new ideas and strategies for the brewing of ice wine.

关键词： Vidal icewine Non-Saccharomyces yeast Saccharomycopsis crataegensis Sensory characterization Aromatic profile Odor activity values

Temporal-spatial analysis of cyromazine in cowpea using liquid chromatography–mass spectrometry coupled with mass spectrometry imaging

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Food quality and Safety 2023年第3期7卷 466-473页

作者： Xuemei Tang Congling Huang Jianxiang Huang Jiale Wang Zhiting Chen Yan Chen Zemiao Xiao Kai Wan institute of quality standard and Monitoring technology for agro-products of Guangdong Academy of Agricultural Sciences Key Laboratory of Testing and Evaluation for Agro-Product Safety and Quality(Guangzhou)Ministry of Agriculture and Rural AffairsNational Reference Laboratory for Agricultural Testing(Pesticide Residues)GuangzhouChina

Background:Cowpea(Vigna unguiculata ***.)is an economically important *** is nutritious and popular with ***,it has been listed as one of the agricultural products of critical concern about safety in China and cyromazine is the major risk ***:This study analyzed the dissipation and permeation kinetics of cyromazine residue in cowpea,to offer a scientific basis for the rational use of pesticide and ensure the safety of agricultural *** and methods:The dissipation and residue level of the systemic insecticide cyromazine on cowpea under field and stored conditions were ***,the spatial distribution of cyromazine was studied using mass spectrometry imaging to visualize the dynamic processes of permeation and migration in the tissues post pesticide ***:The dissipation processing of cyromazine in cowpea was shown to follow the first-order kinetics and half-life was 7.76 d in the *** cowpea,the permeation and migration rate of cyromazine was faster than that in kidney beans and accumulation was mainly in the *** is not safe to apply cyromazine to cowpeas with reference to the application method on kidney ***:These findings present vital data for the determination of risks linked with cowpea consumption and pesticide intake.

关键词： Cyromazine cowpea mass spectrometry imaging pesticide residue

Binding Capacity for Aflatoxin B_1 by Different Adsorbents

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Agricultural Sciences in China 2010年第3期9卷 449-456页

作者： LI Juan-juan SUO De-cheng SU Xiao-ou institute of quality standard and testing technology for agro-Product Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China

In this article, in vitro adsorption of aflatoxin B1 （AFB1） onto different adsorbents was characterized and the result was verified by comparing the growth performance and serum protein levels of broilers exposed to aflatoxin-contamination feed. Main components of adsorbents selected were yeast cell extracts （Product A）, HSCAS （Product B）, and a mixture of yeast product and HSCAS （Product C）, respectively. A total of 240 broilers were assigned to eight treatments, and the effects of three types of adsorbents on growth performance and serum protein levels were evaluated. Results indicated that Product B had the highest in vitro affinity for AFB1, followed by Product C and Product A. Product B bound 97.69% of the AFB1 in solution in 10 min, and it remained over 96.03% in 60 min at pH 8.0. The B-AFB1 complex was much stronger than the other two complexes in vitro condition （P 〈 0.05）. Feed intake （FI） and average daily gain （ADG） decreased （P 〈 0.05） and feed gain ratio increased （P 〈0.05） in the treatment fed aflatoxin-contaminated feed versus treatment on the basal feed. Serum total protein （TP）, albumin （ALB）, and globulin （GLOB） levels were significantly decreased （P〈0.05）. Product B （0.15%） increased growth performance and improved serum protein levels, Product A and Product C were not as effective as Product B. Three adsorbents tested here had sufficient potential to AFBt in some extents and Product B could bind AFB1 more effectively than Product A and Product C. These results indicated that Product B could alleviate some of the AFBt toxic effect in broilers.

关键词： adsorbent AFB 1 absorption efficacy

Time effects of rice straw and engineered bacteria on reduction of exogenous Cu mobility in three typical Chinese soils

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Pedosphere 2022年第5期32卷 665-672页

作者： Hanzhi SHI Dian WEN Yongdong HUANG Shoujun XU Tenghaobo DENG Furong LI Zhichao WU Xu WANG Peihua ZHAO Fuhua WANG Ruiying DU institute of quality standard and Monitoring technology for agro-products Guangdong Academy of Agricultural SciencesGuangzhou 510640(China) Key Laboratory of testing and Evaluation for agro-Product Safety and quality Ministry of Agriculture and Rural AffairsGuangzhou 510640(China) Guangdong Provincial Key Laboratory of quality&Safety Risk Assessment for agro-products Guangzhou 510640(China)

Globally,copper(Cu)accumulation in soils is a major environmental *** organic waste and some bacterial species can readily absorb metals in an eco-friendly manner,and thus are commonly used in metal-contaminated soil *** study investigates the change in Cu fractions during the aging process and the time effects of rice straw(RS)and engineered bacteria(EB)(Pseudomonas putida X4/pIME)on reduction of Cu *** typical Chinese soils(red,cinnamon,and black soils)were incubated with RS or RS+EB in the presence of exogenous Cu for 24 *** soil physicochemical properties,reactive soil components,Cu fractions,and Cu mobility were determined over *** Cu mobility factor(MF)values were the lowest in the black soil(6.4-9.2)because of its high organic carbon and clay *** additions of both RS and RS+EB accelerated Cu stabilization during the aging process in all three *** Cu MF values decreased with time during the initial 20 months;however,the MF values increased thereafter in all soils,which might be due to the reduction of humic substances and amorphous iron oxides and the increase in iron oxides complexed on the organic *** reduction rates of Cu MF were similar after 16,24,and more than 24 months in the red,cinnamon,and black soils,respectively,indicating that RS and RS+EB could limit Cu mobility at different times in various *** RS treatment showed the greatest efficiency in reducing Cu mobility in the red,cinnamon,and black soils after 12,12,and 8 months of incubation,*** RS+EB treatment was more efficient than the RS treatment in the red soil during the initial 8 months of the incubation *** study provides theoretical support for Cu risk assessments and RS supplementation for Cu remediation in different soils.

关键词： aging Cu distribution mobility factor sequential extraction soil incubation

Fusarium mycotoxins:The major food contaminants

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mLife 2024年第2期3卷 176-206页

作者： Zheng Qu Xianfeng Ren Zhaolin Du Jie Hou Ye Li Yanpo Yao Yi An agro‐Environmental Protection institute Ministry of Agriculture and Rural AffairsTianjinChina institute of quality standard and testing technology for agro‐products Shandong Academy of Agricultural SciencesJinanChina

Mycotoxins,which are secondary metabolites produced by toxicogenic fungi,are natural food toxins that cause acute and chronic adverse reactions in humans and *** genus Fusarium is one of three major genera of mycotoxin‐producing ***,fumonisins,and zearalenone are the major Fusarium mycotoxins that occur *** mycotoxins have the potential to infiltrate the human food chain via contamination during crop production and food processing,eventually threatening human *** occurrence and development of Fusarium mycotoxin contamination will change with climate change,especially with variations in temperature,precipitation,and carbon dioxide *** address these challenges,researchers have built a series of effective models to forecast the occurrence of Fusarium mycotoxins and provide guidance for crop *** mycotoxins frequently exist in food products at extremely low levels,thus necessitating the development of highly sensitive and reliable detection *** successful detection methods have been developed to meet the requirements of various situations,and an increasing number of methods are moving toward highthroughput *** Fusarium mycotoxins cannot be completely eliminated,numerous agronomic,chemical,physical,and biological methods can lower Fusarium mycotoxin contamination to safe levels during the preharvest and postharvest *** theoretical innovations and technological advances have the potential to facilitate the development of comprehensive strategies for effectively managing Fusarium mycotoxin contamination in the future.

关键词： climate change Fusarium mycotoxin management strategy mycotoxin detection

Total Synthesis and High Performance Liquid Chromatography Analysis of Phenylethanolamine A

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Chemical Research in Chinese Universities 2017年第4期33卷 598-602页

作者： WANG Tongtong SONG Yinqing WANG Min TANG Xiaoyan ZHOU Jian YANG Mengrui institute of quality standard and testing technology for agro-products Chinese Academy of Agricultural Sciences Beoing 100081 P. R. China Key Laboratory of agro-food Safety and quality Ministry of Agriculture Beijing 100081 P. R. China

A simple, effective and reliable synthetic method was developed to construct beta-agonist phenylethano- lamine A（PEAA） in good yield with 4-nitrobenzyl bromide as the starting material, which underwent SN2 reaction with ethyl acetoacetate, followed by hydrolysis with concentrated HC1 and amination by Lettkart reaction to furnish 4-（4-nitrophenyl）butan-2-amine（A）. A one-pot reaction was studied to construct PEAA skeleton and reduced carbonyl with KBH4, the conditions were optimized, including reaction temperature, reaction time, solvent and the molar ratio of 2-bromo-l-（4-methoxyphenyl）ethanone（B） to A. Meanwhile, a reliable and practical high performance liquid chromography（HPLC） method with UV detector was developed after screening wavelength, chromatographic column and optimization of mobile phase. The optimal conditions of detecting PEAA were water（0.1% formic acid and 0.1% triethylamine）-methanol（volume ratio 44：56） at 278 nm with plus-C^8 column. The above synthetic method would be used to synthesize standard reference materials and advance extensive application of analytical method. In order to ensure food safety and safeguard human health, the analytical method might be used as a standard method in detection and supervision of PEAA.

关键词： Veterinary drug Phenylethanolamme A High performance liquid chromography(HPLC)

Risk assessment and ranking of pesticide residues in Chinese pears

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Journal of Integrative Agriculture 2015年第11期14卷 2328-2339页

作者： LI Zhi-xia NIE Ji-yun YAN Zhen XU Guo-feng LI Hai-fei KUANG Li-xue PAN Li-gang XIE Han-zhong WANG Cheng LIU Chuan-de ZHAO Xu-bo GUO Yong-ze institute of Pomology Chinese Academy of Agricultural Sciences/Laboratory of Quality & Safety Risk Assessment for Fruit (Xingcheng)Ministry of Agriculture Beijing Research Center for Agricultural standards and testing Zhengzhou institute of Pomology Chinese Academy of Agricultural Sciences institute of quality standards & testing technology for agro-products Xinjiang Academy of Agricultural Sciences Yantai Academy of Agricultural Sciences Northwest A&F University institute of Tianjin Agriculture quality standard and testing technology

The presence of pesticide residues in pears is a serious health concern. This study presents the results from a 2-year investigation （2013-2014） that used gas chromatography, GS/MS and UPLC/MS-MS to measure the levels of 104 pesti- cides in 310 pear samples. In 93.2% of the samples, 43 pesticides were detected, of which the maximum residue levels （MRLs） were exceeded in 2.6% of the samples. Multiple residues （two to eight compounds） were present in 69.7% of the samples; one sample contained nine pesticides and one sample contained 10. Only 6.8% of the samples did not contain residues. To assess the health risks, the pesticide residue data have been combined with daily pear consumption data for children and adult populations. A deterministic model was used to assess the chronic and acute exposures based on the Joint Meeting on Pesticide Residues （JMPR） method. A potential acute risk was demonstrated for children in the case of bifenthrin, which was found to be present at 105.36% of the acute reference dose （ARfD） value. The long- term exposure of the Chinese consumer to pesticide residues through the consumption of raw pears was far below the acceptable daily intake （ADI） criterion. Additionally, the matrix ranking scheme was used to classify risk subgroups of pesticides and pear samples. In general, 95.5% of samples were deemed to be safe and nine pesticides were classified as being of a relatively high risk. The findings indicated that the occurrence of pesticide residues in pears should not be considered a serious public health problem. Nevertheless, a more detailed study is required for vulnerable consumer groups, especially children. Continuous monitoring of pesticides in pears and tighter regulation of pesticide residue standards are recommended.

关键词： pear pesticide residue risk assessment intake risk ranking

Heat stress-induced mucosal barrier dysfunction is potentially associated with gut microbiota dysbiosis in pigs

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Animal Nutrition 2022年第1期8卷 289-299页

作者： Bing Xia Weida Wu Wei Fang Xiaobin Wen Jingjing Xie Hongfu Zhang State Key Laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing100193China institute of quality standard and testing technology for agro-products Key Laboratory of Agro-Product Quality and SafetyChinese Academy of Agricultural SciencesBeijing100081China Academy of State Administration of Grain Beijing100037China

Heat stress(HS)can be detrimental to the gut health of *** negative outcomes induced by HS are increasingly recognized as including modulation of intestinal *** turn,the intestinal microbiota is a unique ecosystem playing a critical role in mediating the host stress ***,we aimed to characterize gut microbiota of pigs’exposure to short-term HS,to explore a possible link between the intestinal microbiota and HS-related changes,including serum cytokines,oxidation status,and intestinal epithelial barrier *** findings showed that HS led to intestinal morphological and integrity changes(villus height,serum diamine oxidase[DAO],serum D-lactate and the relative expressions of tight junction proteins),reduction of serum cytokines(interleukin[IL]-8,IL-12,interferongamma[IFN-g]),and antioxidant activity(higher glutathione[GSH]and malondialdehyde[MDA]content,and lower superoxide dismutase[SOD]).Also,16S rRNA sequencing analysis revealed that although there was no difference in microbial a-diversity,some HS-associated composition differences were revealed in the ileum and cecum,which partly led to an imbalance in the production of short-chain fatty acids including propionate acid and valerate *** networks revealed that HS-derived changes in bacterial genera and microbial metabolites,such as Chlamydia,Lactobacillus,Succinivibrio,Bifidobacterium,Lachnoclostridium,and propionic acid,were correlated with oxidative stress,intestinal barrier dysfunction,and inflammation in ***,our observations suggest that intestinal damage induced by HS is probably partly related to the gut microbiota dysbiosis,though the underlying mechanism remains to be fully elucidated.

关键词： Pig Heat stress Intestinal mucosal barrier Bacterial signature Microbeehost interaction

Detection of illegal dyes in foods using a polyethersulfone/multi-walled carbon nanotubes composite membrane as a cleanup method

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Journal of Integrative Agriculture 2018年第3期17卷 716-722页

作者： HE Ya-hui WANG Jing School of Food Science Xinyang College of Agriculture and Forestry Xinyang 464000 P.R. China institute of quality standard and testing technology for agro-products Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China

In this paper, a polyethersulfone （PES）/multi-walled carbon nanotubes （MWCNTs） composite membrane was prepared us- ing phase inversion. The surface morphology and internal structure of the membrane were observed by scanning electron microscopy （SEM）. The effects of MWCNTs content on various aspects of membrane performance such as porosity, water flux, and antifouling characteristics were investigated. Results showed that proper addition of MWCNTs would improve the properties of the membrane. MWCNTs had a strong adsorption capacity for industrial dyes and the composite membrane could be used as an effective method to identify and clean up illegal dyes in foods. In addition, this new method for iden- tifying dyes is rapid： the cleanup procedure in the determination of illegal dyes in foods by the composite membrane was shortened to 30 min or less compared to 6-8 h for traditional methods.

关键词： PES/MWCNTs composite ultrafiltration membrane adsorption industrial dye cleanup determination of illegal dyes in foods

Utilizing modified ubi1 introns to enhance exogenous gene expression in maize(Zea mays L.) and rice(Oryza sativa L.)

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Journal of Integrative Agriculture 2016年第8期15卷 1716-1726页

作者： PAN Yang-yang CHEN Rui ZHU Li WANG Hai HUANG Da-fang LANG Zhi-hong Biotechnology Research institute Chinese Academy of Agricultural Sciences Tianjin institute of Agricultural quality standard and testing technology Tianjin Academy of Agricultural Sciences

supported by the National Natural Science Fund of China （30970231）;the Genetically Modified Organisms Breeding Major Project of China （2014ZX08003001）

关键词： maize intron-mediated enhancement ubil intron intron modification IME signals