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Enriching plant diversity in grasslands by large-scale experimental sward disturbance and seed addition along gradients of land-use intensity

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Journal of plant ecology 2017年第4期10卷 581-591页

作者： Valentin H.Klaus Deborah Schäfer Till Kleinebecker Markus Fischer Daniel Prati Norbert Hölzel institute of landscape ecology Münster UniversityHeisenbergstr.248149 MünsterGermany institute of plant Sciences University of BernAltenbergrain 213013 BernSwitzerland

Aims The relationship between biodiversity and ecosystem functioning is among the most active areas of ecological ***,enhancing the diversity of degraded ecosystems is a major goal in applied restoration *** grasslands,many species may be locally absent due to dispersal or microsite limitation and may therefore profit from mechanical disturbance of the resident *** established a seed addition and disturbance experiment across several grassland sites of different land use to test whether plant diversity can be increased in these ***,the experiment will allow us testing the consequences of increased plant diversity for ecosystem processes and for the diversity of other taxa in real-world ***,we present details of the experimen-tal design and report results from the first vegetation survey 1 year after disturbance and seed ***,we tested whether the effects of seed addition and disturbance varied among grassland depending on their land use or pre-disturbance plant *** A full-factorial experiment was installed in 73 grasslands in three regions across *** were under regular agricul-tural use,but varied in the type and the intensity of management,thereby representing the range of management typical for large parts of Central *** disturbance treatment consisted of disturbing the top 10 cm of the sward using a rotavator or rotary *** addition consisted of sowing a high-diversity seed mixture of regional plant *** species were all regionally present,but often locally absent,depending on the resident vegetation composi-tion and richness of each *** Findings Seven months after sward disturbance,respective plots had significantly increased in bare soil,seedling species richness and number of *** addition had increased plant species richness,but only in combination with sward *** increase in species richness

关键词： grassland restoration germination microsite limitation seed limitation fertilization

Localized micronutrient patches induce lateral root foraging and chemotropism in Nicotiana attenuata

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Journal of Integrative plant Biology 2017年第10期59卷 759-771页

作者： Abigail P. Ferrieri Ricardo A. R. Machado Carla C. M. Arce Danny Kessler lan T. Baldwin Matthias Erb Department of Molecular ecology Max Planck Institute for Chemical Ecology 07745 Jena Germany institute of plant Sciences University of Bern 3013 Bern Switzerland

Nutrients are distributed unevenly in the *** plasticity in root growth and proliferation may enable plants to cope with this variation and effectively forage for essential nutrients. However, how micronutrients shape root architecture of plants in their natural environments is poorly understood. We used a combination of field and laboratory-based assays to determine the capacity of Nicotiana attenuata to direct root growth towards localized nutrient patches in its native environment. plants growing in nature displayed a particular root phenotype consisting of a single primary root and a few long, shallow lateral roots. Analysis of bulk soil surrounding the lateral roots revealed a strong positive correlation between lateral root placement and micronutrient gradients, including copper, iron and zinc. In laboratory assays, the application of localized micronutrient salts close to lateral root tips led to roots bending in the direction of copper and iron. This form of chemotropism was absent in ethylene and jasmonic acid deficient lines,suggesting that it is controlled in part by these two hormones. This work demonstrates that directed root growth underlies foraging behavior, and suggests that chemotropism and micronutrient-guided root placement are important factors that shape root architecture in nature.

关键词： Localized micronutrient patches induce lateral root foraging and chemotropism in Nicotiana attenuata

Cortical Microtubule Reorientation and Its Relation to Cell Surface Texture of Epidermal Cells of Arabidopsis Thaliana Hypocotyls under Simulated Microgravity Conditions

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空间科学学报 2019年第4期39卷 478-488页

作者： XIE Junyan ZHANG Yue ZHENG Huiqiong Center for Excellence in Molecular plant Sciences Institute of Plant Physiology and EcologyChinese Academy of Sciences

Gravitropic curvature growth of Arabidopsis hypocotyls mainly occurred in the rapid growing Elongation Zone(EZI),not in the slow-growing Elongation Zone(EZII).By examining reorientation of Microtubules(MT)and phenotype of the cell wall in the EZI and the EZII of Arabidopsis hypocotyls under normal gravitational condition,it is found that MTs in the rapid growing epidermal cells were mainly in the transverse direction,while those in the non-growing epidermal cells were in the longitudinal ***,this difference in cortical MT arrays between the EZI and EZII cells disappeared when the seedlings were exposed to the simulated microgravity condition on a horizontal *** emission scanning electron microscopy revealed that the surface texture of epidermal cells,like the direction of the MT,in the EZI and the EZII also became similar when exposed to the simulated microgravity *** result indicated that simulate microgravity could modify the potential differentiation between the EZI and the EZII by affecting the orientation of cortical MT in the epidermal cells.

关键词： Arabidopsis Cortical microtubule Cell surface texture Hypocotyls Simulated microgravity

Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer

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Cell Research 2001年第2期11卷 149-155页

作者： HUANG JIAN QIU, ZHI MING WEI, HAI LONG AN, YU XIAN ZHU National Laboratory of plant Molecular Genetics, institute of plant Physiology and ecology, Shanghai institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China National La National Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Chinese Academy of Sciences Shanghai. jqhuang@***

Immature embryos of rice varieties 'Xiushui11' and 'Chunjiang 11' precultured for 4d were infected and transformed by Agrobacterium tumefaciens strain EHA101/pExT7 (containing the spider insecticidal gene). The resistant cant were transferred onto the differentiation medium and plants were regenerated. The transformation frequency reached 56%-72% measured as numbers of Geneticin (G418)-resistant calli produced and 36%-60% measured as numbers of transgenic plants regenerated, respectively. PCR and Southern blot analysis of transgenic plants confirmed that the T-DNA had been integrated into the rice genome. Insect bioassays using T1 transgenic plants indicated that the mortality of the leaffolder (Cnaphalocrasis medinalis) after 7d of leaf feeding reached 38%-61% and the corrected mortality of the striped stem borer (Chilo suppressalis) after 7d of leaf feeding reached 16%-75%. The insect bioassay results demonstrated that the transgenic plants expressing the spider insecticidal protein conferred enhanced resistance to these pests.

关键词： Rice Agrobacterium tumefaciens spider insecticidal gene transgenic plant Leaffolder striped stem borer insect bioassay.

In memory of Prof. Yang Zhong

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Science Bulletin 2017年第24期62卷 1626-1627页

作者： Xiao-Ya Chen institute of plant Physiology and ecology Chinese Academy of Sciences Shanghai 200032 China

Arabidopsis thaliana serves as a model organism for the study of genetics, and is the first flowering plant to get its genome completely sequenced. It also has greatly advanced our understanding of intraspecific genome variations. The extreme and unforgiving environments in Xizang （Tibet）, mainly due to the high altitude, have prompted scientists to ask how wild populations of plants adapt to biotic and abiotic environments.

关键词： In memory Prof. Yang Zhong

CRISPR Primer Designer:Design primers for knockout and chromosome imaging CRISPR-Cas system

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Journal of Integrative plant Biology 2015年第7期57卷 613-617页

作者： Meng Yan Shi-Rong Zhou Hong-Wei Xue National Key Laboratory of plant Molecular Genetics Shanghai Institute of Plant Physiology and Ecologythe Chinese Academy of Sciences

The clustered regularly interspaced short palin- dromic repeats （CRiSPR）-associated system enables biologists to edit genomes precisely and provides a powerful tool for perturbing endogenous gene regulation, modulation of epigenetic markers, and genome architecture. However, there are concerns about the specificity of the system, especially the usages of knocking out a gene. Previous designing tools either were mostly built-in websites or ran as command-line programs, and none of them ran locally and acquired a user- friendly interface. In addition, with the development of CRISPR-derived systems, such as chromosome imaging, there were still no tools helping users to generate specific end-user spacers. We herein present researchers to design primers CRISPR Primer Designer for for CRISPR applications. Theprogram has a user-frfendly interface, can analyze the BLAST results by using multiple parameters, score for each candidate spacer, and generate the primers when using a certain plasmid. In addition, CRISPR Primer Designer runs locally and can be used to search spacer clusters, and exports primers for the CRiSPR-Cas system-based chromosome imaging system.

关键词： Chromosome imaging CRISPR knockout primer software

Senescence-specific Alteration of Hydrogen Peroxide Levels in Arabidopsis thaliana and Oilseed Rape Spring Variety Brassica napus ***

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Journal of Integrative plant Biology 2012年第8期54卷 540-554页

作者： Stefan Bieker Lena Riester Mark Stahl Jürgen Franzaring Ulrike Zentgraf ZMBP (Center for plant Molecular Biology) University of TübingenAuf der Morgenstelle 2872076 Tübingen Germany institute for landscape and plant ecology kologiezentrum 2August-von-Hartmann-Str.370599 StuttgartGermany

In order to analyze the signaling function of hydrogen peroxide （H202） production in senescence in more detail, we manipulated intracellular H202 levels in Arabidopsis thaliala （L.） Heynh by using the hydrogen- peroxide-sensitive part of the Escherichia coil transcription regulator OxyR, which was directed to the cytoplasm as well as into the peroxisomes. H202 levels were lowered and senescence was delayed in both transgenic lines, but OxyR was found to be more effective in the cytoplasm. To transfer this knowledge to crop plants, we analyzed oilseed rape plants Brassica napus L. cv. Mozart for H20~ and its scavenging enzymes catalase （CAT） and ascorbate peroxidase （APX） during leaf and plant development. H202 levels were found to increase during bolting and flowering time, but no increase could be observed in the very late stages of senescence. With increasing H202 levels, CAT and APX activities declined, so it is likely that similar mechanisms are used in oilseed rape and Arabidopsis to control H202 levels. Under elevated CO2 conditions, oilseed rape senescence was accelerated and coincided with an earlier increase in H202 levels, indicating that H202 may be one of the signals to inducing senescence in a broader range of Brassicaceae.

关键词： Arabidopsis thaliana Brassica napus E. coil Oxy-RD intracellular hydrogen peroxide senescence phenotype catalase ascorbatePeroxidase elevated CO2.

Leaf and Ecosystem Gas Exchange Responses of Buffel Grass-Dominated Grassland to Summer Precipitation

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Pedosphere 2015年第1期25卷 112-123页

作者： Mir Zaman HUSSAIN Gnaana SARASWATHI Chhakchhuak LALRAMMAWIA Dennis OTIENO Kailash PALIWAL John TENHUNEN Department of plant ecology University of Bayreuth institute for Genomic Biology University of Illinois Department of plant Sciences Madurai Kamaraj University

Sporadic rain events that occur during summer play an important role in the initiation of biological activity of semi-arid *** understand how ecosystem processes of a buffel grass(Cenchrus ciliaris L.)-dominated grassland respond to summer rain events,an LI 6 400 gas exchange system was used to measure the leaf gas exchange and plant canopy chambers were used to measure net ecosystem CO2exchange(NEE) and ecosystem respiration(Reco), which were made sequentially during periods before rain(dry) and after rain(wet). Gross ecosystem photosynthesis(GEP) was estimated from NEE and Reco fluxes, and light use efficiency parameters were estimated using a rectangular hyperbola model. Prior to the monsoon rain, grassland biomass was non-green and dry exhibiting positive NEE(carbon source) and low GEP values during which the soil water became increasingly scarce. An initial rain pulse(60 mm) increased the NEE from pre-monsoon levels to negative NEE(carbon gain) with markedly higher GEP and increased green biomass. The leaf photosynthesis and leaf stomatal conductance were also improved substantially. The maximum net CO2uptake(i.e.,negative NEE) was sustained in the subsequent period due to multiple rain events. As a result, the grassland acted as a net carbon sink for 20 d after first rain. With cessation of rain(drying cycle), net CO2 uptake was reduced to lower values. High sensitivity of this grassland to rain suggests that any decrease in precipitation in summer may likely affect the carbon sequestration of the semiarid ecosystem.

关键词： biomass CO2 exchange photosynthesis respiration soil moisture stomatal conductance

Acquirement and characterization of a carotenoid mutant (GM309) of Rhodobacter sphaeroides 601

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Science China(Life Sciences) 2004年第1期47卷 52-58页

作者： LIU Yuan ZHANG Wei WU Yongqiang XU Chunhe Shanghai institute of plant Physiology & ecology Shanghai institute for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China Shanghai Institute of Plant Physiology & Ecology Shanghai institute for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China Shanghai Institute of Plant Physiology & Ecology Shanghai institute for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China Shanghai Institute of Plant Physiology & Ecology Shanghai institute for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

A green mutant was obtained among the chemically induced mutants of Rhodobacter sphaeroides 601 (RS601) and named GM309. A blue shift of 20 nm of the carotenoid absorption spectrum was found in the light-harvesting complex II (LH2) of GM309. Different from LH2 of RS601, it was found that the carotenoids in GM309-LH2 changed to be neurosporene by mutation. Neurosporene lacks a conjugate double bond, compared with the spheroidene in RS601-LH2 which has ten conjugate double bonds. As shown by absorption and circular dichroism spectroscopy, the overall structure of GM309-LH2 is little affected by this change. From fluorescence emission spectra, it is found that GM309-LH2 can transfer energy from carotenoids to Bchl-B850 without any change in efficiency. But the efficiency of energy transfer from B800 to B850 in GM309-LH2 is decreased to be 42% of that of the native. This work would provide a novel method to investigate the mechanism of excitation energy transfer in LH2.

关键词： light-harvesting complex, carotenoid, excitation energy transfer, fluorescence.