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In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi

Microsystems & Nanoengineering 2024年第2期10卷 251-267页

作者： Tanja Pajić Katarina Stevanović Nataša V.Todorović Aleksandar J.Krmpot MiroslavŽivić Svetlana Savić-Šević Steva M.Lević Marina Stanić Dejan Pantelić Brana Jelenković Mihailo D.Rabasović institute of Physiology and Biochemistry“Ivan Djaja” Faculty of BiologyUniversity of BelgradeStudentski trg 1611158BelgradeSerbia institute for Biological Research“Siniša Stanković” University of BelgradeNational Institute of the Republic of SerbiaBulevar Despota Stefana 14211000BelgradeSerbia institute of physics belgrade University of BelgradeNational Institute of the Republic of SerbiaPregrevica 11811080BelgradeSerbia University of belgrade Faculty of AgricultureNemanjina Street 611080BelgradeSerbia institute for Multidisciplinary Research University of BelgradeKneza Višeslava 111030BelgradeSerbia

Studying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing pathogens.However,their plasma membrane has been inaccessible for a micron-sized patch-clamp pipette for pA current recordings due to the rigid chitinous cell wall.Here,we report the first femtosecond IR laser nanosurgery of the cell wall of the filamentous fungi,which enabled patch-clamp measurements on protoplasts released from hyphae.A reproducible and highly precise(diffraction-limited,submicron resolution)method for obtaining viable released protoplasts was developed.Protoplast release from the nanosurgery-generated incisions in the cell wall was achieved from different regions of the hyphae.The plasma membrane of the obtained protoplasts formed tight and high-resistance(GΩ)contacts with the recording pipette.The entire nanosurgical procedure followed by the patch-clamp technique could be completed in less than 1 hour.Compared to previous studies using heterologously expressed channels,this technique provides the opportunity to identify new ionic currents and to study the properties of the ion channels in the protoplasts of filamentous fungi in their native environment.

关键词： patch recording laser

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Physiological strategies in wild rodents:immune defenses of commensal rats

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Integrative Zoology 2024年第3期19卷 350-370页

作者： Ivana MIRKOV Dina TUCOVIC Jelena KULAS Anastasija MALESEVIC Dragan KATARANOVSKI Milena KATARANOVSKI Aleksandra POPOV ALEKSANDROV Immunotoxicology Group Department of EcologyInstitute for Biological Research“Sinisa Stankovic”—National Institute of Republic of SerbiaUniversity of BelgradeBelgradeSerbia

The importance of issues associated with urban/commensal rats and mice(property damage,management costs,and health risks)press upon research on these animals.While the demography of commensal rodents is mostly studied,the need for understanding factors influencing their natural morbidity/mortality is also stressed.In this respect,more attention is expected to be paid to immunity,the physiological mechanism of defense against host survival threats(pathogens,parasites,diseases).Commensal rats and mice carry numerous pathogens that evoke diverse immune responses.The state of immunity in commensal house mice is studied in great detail,owing to the use of laboratory strains in biomedical research.Because commensal rats are,compared to mice,carriers of more zoonotic agents,rats’immunity is studied mainly in that context.Some of these zoonotic agents cause chronic,asymptomatic infections,which justified studies of immunological mechanisms of pathogen tolerance versus clearance regulation in rats.Occurrence of some infections in specific tissues/organs pressed upon analysis of local/regional immune responses and/or immunopathology.A survey of immunological activity/responses in commensal rats is given in this review,with mention of existing data in commensal mice.It should throw some light on the factors relevant to their morbidity and lifespan,supplementing the knowledge of commensal rodent ecology.

关键词： commensal rats and mice immunopathology infection inflammation tolerance

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Role of fullerenols derivative 3HFWC in the treatment of Alzheimer's disease

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Neural Regeneration Research 2024年第8期19卷 1641-1642页

作者： Sanja Ivkovic Djuro Koruga Department of Molecular Biology and Endocrinology “VINCA”Institute of Nuclear Sciences-National Institute of Republic of SerbiaUniversity of BelgradeBeogradSrbija TFT Nano Center BelgradeSerbia

Fullerenes:The extensive development of nanoscience that has marked this century continues to evolve,producing new materials,structures,and devices for the treatments of diverse pathologies.Fullerenes are a family of nanoparticles with great applicative promise due to their small size(approximately 1 nm in diameter),structure,and capacity to cross biological barriers.

关键词： treatment evolve size

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Global warming scenarios for the Eastern Adriatic Sea indicate a higher risk of invasiveness of non-native marine organisms relative to current climate conditions

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Marine Life Science & Technology 2024年第1期6卷 143-154页

作者： Branko Glamuzina Lorenzo Vilizzi Marina Piria Ante Zuljevic Ana Bratos Cetinic Ana Pesic Branko Dragicevic Lovrenc Lipej Marijana Pecarevic Vlasta Bartulovic Sanja Grđan Ivan Cvitkovic Tatjana Dobroslavic Ana Fortic Luka Glamuzina Borut Mavric Jovana Tomanic Marija Despalatovic Domen Trkov Marina Brailo Scepanovic Zoran Vidovic Predrag Simonovic Sanja Matic-Skoko Pero Tutman Department of Applied Ecology University of DubrovnikDubrovnikCroatia Department of Ecology and Vertebrate Zoology Faculty of Biology and Environmental ProtectionUniversity of LodzLodzPoland Faculty of Agriculture Department of FisheriesApicultureWildlife Management and Special ZoologyUniversity of ZagrebZagrebCroatia institute of Oceanography and Fisheries SplitCroatia institute of Marine Biology University of MontenegroKotorMontenegro National institute of Biology Marine Biology Station PiranPiranSlovenia Teacher Education Faculty University of BelgradeBelgradeSerbia Faculty of Biology University of BelgradeBelgradeSerbia

Globally,marine bioinvasions threaten marine ecosystem structure and function,with the Mediterranean Sea being one of the most affected regions.Such invasions are expected to increase due to climate change.We conducted a risk screening of marine organisms(37 fishes,38 invertebrates,and 9 plants),both extant and‘horizon’(i.e.,not present in the area but likely to enter it).Based on expert knowledge for the Eastern Adriatic Sea coasts of Slovenia,Croatia,and Montenegro,screenings were conducted under both current and predicted climate conditions indicating with an increase in sea surface temperature and salinity of the Adriatic Sea together with changes in precipitation regime.Our aims were to:(1)identify non-native extant and horizon marine species that may pose threats to native biodiversity and(2)evaluate the risk of invasiveness of the selected species under current and predicted climate conditions.Of the 84 species screened,there was an increase in those ranked as‘high risk’from 33(39.3%)under current climate conditions and to 47(56.0%)under global warming scenarios.For those ranked as‘very high’risk,the increase was from 6(7.1%)to 21(25.0%).Amongst the screened species,the already established high-risk species Pacific oyster Magallana gigas and Atlantic blue crab Callinectes sapidus represent a threat to ecosystem services.Given the under-representation of marine species in the current European Union List,the species we have ranked as high to very high risk should be included.

关键词： AS-ISK Early detection Introduction vectors/pathways Invasive species Rapid response

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Deferred revascularization in diabetic patient according to combined invasive functional and intravascular imaging data:A case report

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World Journal of Clinical Cases 2024年第13期12卷 2269-2274页

作者： Arif Al Nooryani Wael Aboushokka Branko Beleslin Biljana Nedeljkovic-Beleslin Department of Cardiology Al Qassimi HospitalSharjah 1234United Arab Emirates Department of Cardiology Medical FacultyUniversity of BelgradeBelgrade 11000Serbia institute of Endocrinology Diabetes and Metabolic DisordersUniveristy Clinical Center of SerbiaBelgrade 11000Serbia Department of Internal Medicine/Endocrinology Medical facultyUniversity of BelgradeBelgrade 11000Serbia

BACKGROUND Invasive functional evaluation by fractional flow reserve(FFR)is considered as a gold standard for the evaluation of intermediate coronary stenosis.However,in patients with diabetes due to accelerated progression of atherosclerosis the outcome may be worse even in the presence of negative functional testing.CASE SUMMARY We present a case of 55-year-old male diabetic patient who was admitted for chest pain.Diagnostic coronary angiography disclosed 2 intermediate stenoses of the obtuse marginal branch with no evidence of restenosis on previously implanted stent.Patient undergone invasive functional testing of intermediate lesion with preserved FFR(0.88),low coronary flow reserve(1.2)and very high index of microvascular resistance(84).Due to discrepancy in invasive functional parameters,intravascular imaging with optical coherence tomography showed fibrotic stenoses without signs of thin-sup fibroatheroma.Because of the preserved FFR and no signs of vulnerable plaque,the interventional procedure was deferred and the patient continued with optimal medications.CONCLUSION Combined functional and anatomic imaging of intermediate coronary stenosis in diabetic patients represent comprehensive contemporary decision pathway in the management of the patients.

关键词： Fractional flow reserve Coronary flow reserve Index of microvascular resistance Optical coherence tomography Intermediate coronary stenosis Case report

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Motion of charged particles in bright squeezed vacuum

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Light(Science & Applications) 2024年第2期13卷 347-353页

作者： Matan Even Tzur Oren Cohen Solid State institute and physics Department Technion-Israel Institute of TechnologyHaifa3200003Israel

The motion of laser-driven electrons quivers with an average energy termed pondermotive energy.We explore electron dynamics driven by bright squeezed vacuum(BSV),finding that BSV induces width oscillations,akin to electron quivering in laser light,with an equivalent ponderomotive energy.We identify closed and open trajectories of the electronic width that are associated with high harmonic generation and above-threshold ionization,respectively,similarly to trajectories of the electron position when its motion is driven by coherent light.In the case of bound electrons,the width oscillations may lead to ionization with noisy sub-cycle structure.Our results are foundational for strong-field and free-electron quantum optics,as they shed light on ionization,high harmonic generation,and nonlinear Compton scattering in BSV.

关键词： structure vacuum ionization

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Two-dimensional investigation of characteristic parameters and their gradients for the self-generated electric and magnetic fields of laser-induced zirconium plasma

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Plasma Science and Technology 2024年第5期26卷 138-155页

作者： Tayyaba SAJID Shazia BASHIR Mahreen AKRAM Maira RAZZAQ Khaliq MAHMOOD Centre for Advanced Studies in physics Institute of PhysicsGovernment College University LahoreLahore 54000Pakistan Department of physics Institute of PhysicsGovernment College University LahoreLahore 54000Pakistan

Two-dimensional diagnosis of laser-induced zirconium(Zr)plasma has been experimentally performed using the time-of-flight method by employing Faraday cups in addition to electric and magnetic probes.The characteristic parameters of laser-induced Zr plasma have been evaluated as a function of different laser irradiances ranging from 4.5 to 11.7 GW cm-2 at different axial positions of 1–4 cm with a fixed radial distance of 2 cm.A well-supporting correlation between the plume parameters and the laser-plasma-produced spontaneous electric and magnetic(E and B)fields was established.The measurements of the characteristic parameters and spontaneously induced fields were observed to have an increasing trend with the increasing laser irradiance.However,when increasing the spatial distance in both the axial and radial directions,the plasma parameters(electron/ion number density,temperature and kinetic energy)did not show either continuously increasing or decreasing trends due to various kinetic and dynamic processes during the spatial evolution of the plume.However,the E and B fields were observed to be always diffusing away from the target.The radial component of electron number densities remained higher than the axial number density component,whereas the axial ion number density at all laser irradiances and axial distances remained higher than the radial ion number density.The higher axial self-generated electric field(SGEF)values than radial SGEF values are correlated with the effective charge-separation mechanism of electrons and ions.The generation of a self-generated magnetic field is observed dominantly in the radial direction at increasing laser irradiance as compared to the axial one due to the deflection of fast-moving electrons and the persistence of two-electron temperature on the radial axis.

关键词： Faraday cup axial and radial expansion space-charge effect laser-induced zirconium plasma two-electron temperature distribution self-generated electric and magnetic fields

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Splenic hamartomas in children

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World Journal of Clinical Cases 2024年第11期12卷 1909-1917页

作者： Maja Milickovic Petar Rasic Sofija Cvejic Dejana Bozic Djordje Savic Tanja Mijovic Sava Cvetinovic Slavisa M Djuricic Department of Abdominal Surgery Mother and Child Health Care Institute of Serbia"Dr.Vukan Cupic"Belgrade 11000Serbia Faculty of Medicine University of BelgradeBelgrade 11000Serbia Department of Radiology The Children’s University HospitalBelgrade 11000Serbia Department of Clinical Pathology Mother and Child Health Care Institute of Serbia"Dr.Vukan Cupic"Belgrade 11000Serbia Faculty of Medicine University of Banja LukaBanja Luka 78000Bosnia and Herzegovina

Splenic hamartomas(SHs)are uncommon,benign vascular lesions of unclear etiology and are mostly found incidentally on abdominal images,at surgery,or at autopsy.Since the first case description,in 1861,less than 50 pediatric SH cases have been reported in the literature.In this article,we have performed an analysis of all SH cases in children published in the literature to date and presented our case of an 8-year-old male with SH.These lesions in children were shown to cause symptoms more often than in the adult population.The observed SH sizes in children ranged from a few millimeters to 18 cm,and the symptomatic lesions were mostly larger or multiple.The most common clinical finding was splenomegaly.Signs of hypersplenism were present in children with a single SH larger than 4.5 cm(diameter range:4.5-18.0 cm)and in those with multiple hamartomas,ranging from a few millimeters to 5 cm.Eighty percent of patients with available laboratory findings had hematological abnormalities such as anemia,thrombocytopenia,or pancytopenia.Other symptoms and signs included abdominal pain,recurrent infections,fever,night sweats,lethargy,growth retardation,and weight loss.The use of multiple imaging modalities may suggest the preoperative diagnosis of a splenic mass in children and determine the therapeutic approach.However,the final diagnosis of SH relies on histopathological evaluation.Surgery,including total or partial splenectomy(PS),is the mainstay of SH management.Milickovic M et al.Splenomas in children WJCC https://*** 1910 April 16,2024 Volume 12 Issue 11 Although total splenectomy carries a greater risk of overwhelming post-splenectomy infection than PS it has remained the most performed surgical procedure in children with SH.In the majority of pediatric patients with symptomatic SH,resolution of symptoms and resolution or improvement of cytopenias occurred after surgical treatment.

关键词： Splenic hamartoma Pediatric Splenoma Clinical features Radiological features Histopathology Treatment

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Consistency between domain wall oscillation modes and spin wave modes in nanostrips

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Chinese physics B 2024年第6期33卷 511-516页

作者：董新伟吴振江 Department of physics and institute of Theoretical physics and Astrophysics Xiamen UniversityXiamen 361005China

Investigations on domain wall(DW) and spin wave(SW) modes in a series of nanostrips with different widths and thicknesses have been carried out using micromagnetic simulation. The simulation results show that the frequencies of SW modes and the corresponding DW modes are consistent with each other if they have the same node number along the width direction. This consistency is more pronounced in wide and thin nanostrips, favoring the DW motion driven by SWs.Further analysis of the moving behavior of a DW driven by SWs is also carried out. The average DW speed can reach a larger value of ~ 140 m/s under two different SW sources. We argue that this study is very meaningful for the potential application of DW motion driven by SWs.

关键词： micromagnetic simulation domain wall spin wave

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Pulsed THz radiation under ultrafast optical discharge of vacuum photodiode

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Frontiers of Optoelectronics 2024年第2期17卷 99-112页

作者： Aleksandr Ushakov Kseniia Mamaeva Leonid Seleznev Georgy Rizaev Vladimir Bukin Timophey Dolmatov Pavel Chizhov Vladimir Bagdasarov Sergey Garnov Prokhorov General physics institute of the Russian Academy of Sciences Moscow 119991Russia Physical institute Russian Academy of SciencesMoscow 119991Russia Moscow institute of physics and Technology Dolgoprudny 141700Russia Russian institute for Scientific and Technical Information Moscow 125190Russia

In this paper,we first present an experimental demonstration of terahertz radiation pulse generation with energy up to 5 pJ under the electron emission during ultrafast optical discharge of a vacuum photodiode.We use a femtosecond optical excitation of metallic copper photocathode for the generation of ultrashort electron bunch and up to 45 kV/cm external electric field for the photo-emitted electron acceleration.Measurements of terahertz pulses energy as a function of emitted charge density,incidence angle of optical radiation and applied electric field have been provided.Spectral and polarization characteristics of generated terahertz pulses have also been studied.The proposed semi-analytical model and simulations in COMSOL Multiphysics prove the experimental data and allow for the optimization of experimental conditions aimed at flexible control of radiation parameters.

关键词： Electron emission Vacuum photodiode Ultrafast excitation Terahertz

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