检索结果-南通市图书馆

Inhibition of the NLRP3 inflammasome attenuates spiral ganglion neuron degeneration in aminoglycoside-induced hearing loss

引用

Neural Regeneration Research 2025年第10期20卷 3025-3039页

作者： Jia Fang Zhuangzhuang Li Pengjun Wang Xiaoxu Zhang Song Mao Yini Li Dongzhen Yu Xiaoyan Li Yazhi Xing Haibo Shi Shankai Yin Department of Otolaryngology Head and Neck Surgery Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of MedicineOtolaryngology Institute of Shanghai Jiao Tong UniversityShanghai Key Laboratory of Sleep Disordered BreathingShanghaiChina Department of otorhinolaryngology-Head and Neck Surgery Shanghai Children’s HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina

Aminoglycosides are a widely used class of antibacterials renowned for their effectiveness and broad antimicrobial ***,their use leads to irreversible hearing damage by causing apoptosis of hair cells as their direct *** addition,the hearing damage caused by aminoglycosides involves damage of spiral ganglion neurons upon *** investigate the mechanisms underlying spiral ganglion neuron degeneration induced by aminoglycosides,we used a C57BL/6J mouse model treated with *** found that the mice exhibited auditory deficits following the acute loss of outer hair *** ganglion neurons displayed hallmarks of pyroptosis and exhibited progressive degeneration over *** profiling of these neurons showed significant upregulation of genes associated with inflammation and immune response,particularly those related to the NLRP3 *** of the canonical pyroptotic pathway in spiral ganglion neurons was observed,accompanied by infiltration of macrophages and the release of proinflammatory *** intervention targeting NLRP3 using Mcc950 and genetic intervention using NLRP3 knockout ameliorated spiral ganglion neuron degeneration in the injury *** findings suggest that NLRP3 inflammasome-mediated pyroptosis plays a role in aminoglycoside-induced spiral ganglion neuron *** of this pathway may offer a potential therapeutic strategy for treating sensorineural hearing loss by reducing spiral ganglion neuron degeneration.

关键词： degeneration hearing loss macrophages Mcc950 neuroinflammation NLRP3 inflammasome ototoxicity pyroptosis sensorineural hearing loss spiral ganglion neuron

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Efficient generation of all ESC-derived mice carrying a homozygous lethal mutation through eight-cell embryo injection

引用

Science China Life Sciences 2025年

作者： Xidi Yin Meng Yan Yanbo Cheng Zhoujie Li Chong Cui Yingyi Wang Nan Liu Yilai Shu Jinsong Li Qing Li Key Laboratory of Multi-Cell Systems Shanghai Key Laboratory of Molecular Andrology Shanghai Institute of Biochemistry and Cell Biology Center for Excellence in Molecular Cell Science Chinese Academy of Sciences School of Life Science and Technology ShanghaiTech University Animal Core Facility Shanghai Institute of Biochemistry and Cell Biology Center for Excellence in Molecular Cell Science Chinese Academy of Sciences ENT institute and Department of otorhinolaryngology Eye & ENT Hospital State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Fudan University Key Laboratory of Systems and Health Science of Zhejiang Province School of Life Science Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences

Dear Editor,Large-scale phenotyping efforts have shown that placental defects correlate strongly with embryonic lethal mouse mutants(Perez-Garcia et al., 2018). To rescue normal development of these mutant embryos, tetraploid complementation can be a reliable strategy, in which,tetraploid cells form extraembryonic lineages thus providing the embryo with a wild-type placenta(Nagy et al., 1990).

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Bhlhe40 protects cochlear hair cell-like HEI-OC1 cells against H_(2)O_(2) ‑triggered oxidative injury

引用

BIOCELL 2024年第6期48卷 991-999页

作者： LITING WEN XIAOXIA ZENG PEIXIONG CHEN DAPENG ZHAO YANGYANG LI XIANHAI ZENG Department of otorhinolaryngology Longgang Otorhinolaryngology Hospital and Shenzhen Key Laboratory of OtorhinolaryngologyShenzhen Institute of OtorhinolaryngologyShenzhenChina

Background:Cochlear hair cell injury is a common pathological feature of hearing *** basic helix-loop-helix family,member e40(Bhlhe40),a gene belonging to the basic helix-loop-helix(bHLH)family,exhibits strong transcriptional repression ***:Oxidative damage,in House Ear institute-Organ of Corti 1(HEI-OC1)cells,was caused using hydrogen peroxide(H2O2).The Ad-Bhlhe40 particles were constructed to overexpress Bhlhe40 in HEI-OC1 *** assays including cell counting kit-8(CCK-8),terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay(TUNEL),ﬂow cytometry,immunoﬂuorescence,and corresponding commercial kits were employed to investigate the impacts of Bhlhe40 on cell viability,apoptosis,oxidative stress levels,mitochondrial membrane potential and cellular ***,a dual-luciferase reporter assay was performed to conﬁrm the targeting of the histone deacetylases 2(Hdac2)by ***:The results revealed that Bhlhe40 was downregulated in H_(2)O_(2)-treated HEI-OC1 cells,but its overexpression improved cell viability and mitigated H_(2)O_(2)-induced oxidative injury in HEI-OC1 cells with increase of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GPx)activities and decrease of reactive oxygen species(ROS)***,overexpression of Bhlhe40 suppressed H_(2)O_(2)-triggered cell senescence,as evidenced by the fact that the upregulation of P53,P21,and P16 in HEI-OC1 cells treated with H2O2 were all alleviated by Bhlhe40 *** we further veriﬁed that overexpression of Bhlhe40 could inhibit the expression of Hdac2,which may be related to the repression of Hdac2 ***:This study suggests that Bhlhe40 plays a protective role against senescence and oxidative damage in cochlear hair cells exposed to H2O2.

关键词： Bhlhe40 Oxidative injury Cochlear hair cell Histone deacetylases 2

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Development of the inner ear and regeneration of hair cells after hearing impairment

引用

Fundamental Research 2025年第1期5卷 203-214页

作者： Bing, Xin Liu, Chengcheng Cao, Xue Li, Chengzhilin Gao, Xiaochen Zhu, Fangyuan Wu, Xinhao Guo, Na Hu, Houyang Xia, Ming Zhao, Miaoqing Department of Otolaryngology Shandong Provincial Hospital Shandong University Jinan 250021 China Department of Central Laboratory Shandong Provincial Hospital Affiliated to Shandong First Medical University Jinan 250021 China Medical Science and Technology Innovation Center Shandong First Medical University & Shandong Academy of Medical Sciences Jinan 271000 China Department of Otolaryngology Shandong Provincial Hospital Affiliated to Shandong First Medical University Jinan 250021 China NHC Key Laboratory of otorhinolaryngology Jinan 250021 China Department of Pathology Shandong Cancer Hospital and Institute Shandong First Medical University and Shandong Academy of Medical Sciences Jinan 250117 China

Hearing loss, as a sensory disorder, is the most common occurrence among humans, which has received increasing attention from society. It is mainly caused by the damage of inner ear hair cells (HCs) or the degeneration of spiral ganglion neurons. In mammals, cochlear HCs cannot regenerate naturally after injury, leading to irreversible hearing loss. Therefore, HCs are essential for hearing protection. In recent years, the protection of drug-related ototoxicity, inner ear stem cells, gene therapy, new materials, and signal regulation have become important ways to develop regeneration strategies of HCs. An in-depth study of the causes of the occurrence and development of hearing impairment and the regeneration of hearing loss for effective prevention, discovery, and treatment of deafness has great significance. This review aimed to analyze the development of the inner ear and summarize the related factors leading to HCs injury and the research progress of regeneration after injury. © 2023

关键词： Development Hearing impairment Hearing protection Inner ear hair cells Regeneration

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Expression of HSP70 in Peripheral Lymphocytes of the Patients with Allergic Rhinitis

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2003年第3期23卷 310-312页

作者：刘立思肖成峰张明程磊王鄂芬邬堂春 Department of otorhinolaryngology institute of Occupational Medicine

The expression levels of heat shock protein 70 (HSP70) from peripheral lymphocytes of the patients with allergic rhinitis (AR) and the clinical implication were investigated In the morning, 3 ml of fasting venous blood was taken out The lymphocytes were isolated by using Ficoll-Hypaque and the expression of HSP70 in the lymphocytes was detected by using Western blot In the AR patients the HSP70 level (41 49±15 77 integrated optical density, IOD) were significantly higher than that in the control group (23 89±10 13 IOD, P< 0 05) Western blot demonstrated that HSP70 bands in AR patients were more intensive than those in the control group It was concluded that the elevated HSP70 level in peripheral lymphocytes of the AR patients might contribute to the development of AR

关键词： allergic rhinitis lymphocyte heat shock protein 70

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Current AAV-mediated gene therapy in sensorineural hearing loss

引用

Fundamental Research 2025年第1期5.0卷 192-202页

作者： Qi, Jieyu Fu, Xiaolong Zhang, Liyan Tan, Fangzhi Li, Nianci Sun, Qiuhan Hu, Xiaojie He, Zuhong Xia, Ming Chai, Renjie State Key Laboratory of Bioelectronics Department of Otolaryngology Head and Neck Surgery Zhongda Hospital School of Life Sciences and Technology Advanced Institute for Life and Health Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research Southeast University Nanjing 210096 China Department of otorhinolaryngology-Head and Neck Surgery Zhongnan Hospital of Wuhan University Wuhan 430071 China Department of Otolaryngology Shandong Provincial Hospital Affiliated to Shandong First Medical University Jinan 250021 China Medical Science and Technology Innovation Center Shandong First Medical University & Shandong Academy of Medical Sciences Jinan 250021 China Co-Innovation Center of Neuroregeneration Nantong University Nantong 226001 China institute for Stem Cell and Regeneration Chinese Academy of Science Beijing 100101 China Beijing Key Laboratory of Neural Regeneration and Repair Capital Medical University Beijing 100069 China Department of Otolaryngology Head and Neck Surgery Sichuan Provincial People's Hospital University of Electronic Science and Technology of China Chengdu 610072 China

The number of patients with hearing loss is on the rise due to congenital abnormalities, degenerative changes in old age, and acquired injuries such as virus or ototoxic drug-induced diseases. Hearing loss is a refractory and disabling disease that has serious negative effects on quality of life. The pathology of hearing loss in the inner ear is characterized by varying degrees of damage to the cochlear sensory epithelium cells (such as hair cells and supporting cells), stria vascularis (including marginal, intermediate and basal cells) and spiral ganglion neurons. Regeneration or direct repair of damaged cells in the inner ear is an effective way to treat sensorineural deafness. It is currently possible to regenerate hair cells to treat sensorineural hearing loss by FX-322, a small molecule drug in clinical trials. With the development of genetic engineering technology, gene therapy has brought a promising treatment strategy for many previously intractable diseases. Gene therapy has been regarded as a promising method in the treatment and rehabilitation of sensorineural hearing loss, and recombinant adeno-associated virus gene therapy has been widely used in fundamental research into hearing loss treatments. At present, gene therapy for hearing loss is transitioning from feasibility studies to explorations of its safety and its therapeutic potential. The present article reviews the concepts, strategies, and applications of gene therapy mediated by recombinant adeno-associated viruses in the field of hearing loss treatment. © 2022

关键词： Adeno-associated virus Auditory repair Cochlea Gene therapy Hearing loss

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Healthcare Algorithms by Wearable Inertial Sensors: A Survey

引用

China Communications 2015年第4期12卷 1-12页

作者： AO Buke FANG Gaoli WANG Yongcai SONG Lei YANG Zhiqi School of Information and Communication Engineering Beijing University of Post and Telecommunication Beijing key laboratory of nasal diseases Beijing Institute of Otorhinolaryngology Department of otorhinolaryngology Head and Neck Surgery Beijing Di Tan HospitalCapital Medical University institute for Interdisciplinary Information Sciences Tsinghua University

Wearable smart devices, such as smart watch, wristband are becoming increasingly popular recently. They generally integrate the MEMS-designed inertial sensors, including accelerometer, gyroscope and compass, which provide a convenient and inexpensive way to collect motion data of users. Such rich, continuous motion data provide great potential for remote healthcare and decease diagnosis. Information processing algorithms play the critical role in these approaches, which is to extract the motion signatures and to access different kinds of judgements. This paper reviews key algorithms in these areas. In particular, we focus on three kinds of applications: 1) gait analysis; 2) fall detection and 3) sleep monitoring. They are the most popular healthcare applications based on the inertial data. By categorizing and introducing the key algorithms, this paper tries to build a clear map of how the inertial data are processed; how the inertial signatures are defined, extracted, and utilized in different kinds of applications. This will provide a valuable guidance for users to understand the methodologies and to select proper algorithm for specifi c application purpose.

关键词： healthcare algorithms wearable inertial sensors IMU gait analysis falldetection sleep monitoring

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The protective effects against noise trauma by sound conditioning in rats

引用

Journal of Otology 2009年第1期4卷 59-60页

作者： ZHAI Suo-qiang,GUO Wei-wei,YU Ning institute of otorhinolaryngology,General Hospital of PLA,Beijing 100853 institute of otorhinolaryngology General Hospital of PLA Beijing 100853

Objective To investigate the protective effects of sound conditioning against subsequent high -level noise trauma in *** Rats were exposed to a 4 kHz octave band noise at 95 dB SPL for 10 hours,then to a traumatic exposure dose(105 dB SPL for 13 hours) delivered 12h *** animals were exposured to the traumatic dose *** thresholds were obtained before and after noise *** Animals that had been sound conditioned demonstrated less ABR threshold shift compared to those that had *** Moderate level sound exposure appears to have a toughening effect on the rat cochlea(or'conditioning') leading to decreased hearing loss from subsequent traumatic exposure.

关键词： sound conditioning noise trauma rat

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Development of a script of phonemically balanced monosyllable lists of Mandarin-Chinese

引用

Journal of Otology 2010年第1期5卷 8-19页

作者： JI Fei, CHEN Aiting, ZHAO Yang, XI Xin, HAN Dongyi Departments of otorhinolaryngology-Head and Neck Surgery, institute of otorhinolaryngology, General Hospital of People’s Liberation Army, Beijing 100853, China Departments of otorhinolaryngology–Head and Neck Surgery Institute of Otorhinolaryngology General Hospital of People’s Liberation Army Beijing 100853 China

Objectives To develop a set of monosyllable audiometry test material that can be used in speech recognition testing in Mandarin Chinese. Materials and Methods In an effort to improve reliability and efficiency in clinical practice, a set of 30 test lists of 25 monosyllable test items each was designed with consideration of the following: length of the list, phonemical balance (PB), word utility frequency, coverage of common words, and equivalency among lists. The 30 lists contained 750 monosyllabic words and were constructed on the basis of Chinese phoneme distribution probability summarized in the Manual of Acoustic. The occurrence incidence of the 22 consonants, 36 vowels, and 4 tones of the Chinese Mandarin characters in the 30 test lists were calculated. To achieve PB, 489 monosyllables were selected from the 2500 most commonly used Chinese characters and the 4000 most commonly used Chinese phrases to compile the 30 monosyllable test lists using a computer algorithm with manual adjustment. Results Thirty phonemical balanced word lists of common Mandarin Chinese monosyllabic words were compiled. Each list consisted of 25 monosyllables. A total of 489 phonemes (consonants, vowels and tones) were included in the set. Conclusions This set of lists can potentially be used as the basic lists for future development of Mandarin PB monosyllable speech test materials.

关键词： Audiometry Speech Acoustics Mandarin Chinese

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

INJURY TO THE MIDDLE EAR DUE TO HIGH-INTENSITY SOUND

引用

Chinese Journal of Acoustics 1989年第1期8卷 65-71页

作者： Meng, Zhaohui Mingkun, Cheng Shen, Hao Beijing Inst of otorhinolaryngology China

In recent ten odd years, numerous experiments have been made on animals with high impulse noise and high - intensity continuous broadband noise, respectively. Subsequently a series of observations on human ears expose... 详细信息

关键词： Acoustic Wave Effects Health Care

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：