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中国畜牧兽医学会家畜内科学分会2009年学术研讨会

作者：潘家强 institute of nutritional and metabolic disorder in domestic animals and fowls Nanjing Agricultural University

Objective:The present study was conducted to investigate the effect of early feed restriction on protein kinase Cα(PKCα) expression in pulmonary arterioles,which has been revealed to promote pulmonary vascular remodelling in pulmonary hypertensive ***:A total of 270 day-old mixedsex commercial broilers were randomly distributed to a normal temperature control group(NT),a low temperature control group(LT) and low temperature plus feed restriction group(LR).The PHS incidence, the right/total ventricular weight ratio(RV/TV),the vessel wall area/vessel total area ratio(WA/TA),the mean media thickness in pulmonary arterioles(mMTPA) and the expression of PKCαin the pulmonary arterioles were measured ***:Low temperature significantly increased the PHS *** RV/TV,WA/TA and mMTPA values of group LT were significantly elevated compared to those of group NT on d 35 and *** LT chickens had increased PKCαexpression compared to their NT counterparts on d 28 and *** restriction reduced the PHS mortality,RV/TV,WA/TA and mMTPA in cold-exposed *** LR chickens had much lower PKCαexpression in pulmonary arterioles than the LT ***:Early time feed restriction inhibited pulmonary vascular remodelling in broilers, which might be partly attributed to reduced PKCαexpression in pulmonary arterioles.

关键词： Early feed restriction Pulmonary hypertension syndrome PKCα Pulmonary vascular remodelling

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The injury effect of oxygen free radicals in vitro on cultured pulmonary artery endothelial cells from broiler

The injury effect of oxygen free radicals in vitro on cultur...

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中国畜牧兽医学会家畜内科学分会2009年学术研讨会

作者：潘家强 institute of nutritional and metabolic disorders in domestic animals and fowls Nanjing Agricultural University

Pulmonary artery endothelial cells(PAEC) of broiler were isolated by the method of tissue *** of the cells were performed with the morphological feature and immunocytochemical stain using specific antiserum against factorⅧrelated ***/Xanthine oxidase(X/XO) served as the oxygen free radical(OFR) generating *** vitro model of oxidative injury of PAEC was established based on the X/XO *** effect of OFR on the growth and viability of PAEC was determined with methylthiazol tetrazolium(MTT) colorimetric *** (MDA,a product of lipid peroxidation) in culture medium of PAEC was detected by a thiobarbituric acid colorimetric *** result showed that PAEC could be survived in vitro and had been subcultured for 5-6 *** and immunohistochemical observing of cultured cells demonstrated specific characteristics of endothelial *** were severely damaged by *** viability of cells was inhibited by X/XO system and dose-dependent decrease of cell viability was found with increased XO *** promoted the lipid peroxidation of PAEC and increased the MDA concentration in culture *** results suggest that OFR had an injury effect on the endothelial cells of pulmonary artery in broiler,which was consistent with the result in vivo,and may play an important role in the pathogenesis of pulmonary hypertension syndrome in broiler.

关键词： Broiler Pulmonary artery endothelial cells In vitro culture Oxygen free radical

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Phenethyl isothiocyanate as an anti-nutritional factor attenuates deoxynivalenol-induced IPEC-J2 cell injury through inhibiting ROSmediated autophagy

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Animal Nutrition 2022年第1期8卷 300-309页

作者： Shuiping Liu Xinru Mao Lei Ge Lili Hou Guannan Le Fang Gan Lixin Wen Kehe Huang College of Veterinary Medicine Nanjing Agricultural UniversityNanjing210095China institute of nutritional and metabolic disorders in domestic animals and fowls Nanjing Agricultural UniversityNanjing210095China MOE Joint International Research Laboratory of Animal Health and Food Safety College of Veterinary MedicineNanjing Agricultural UniversityNanjing210095China College of Veterinary Medicine Hunan Agricultural UniversityChangsha410128China

Deoxynivalenol(DON)is considered to be the most harmful mycotoxin that affects the intestinal health of animals and *** isothiocyanate(PEITC)in feedstuff is an anti-nutritional factor and impairs nutrient digestion and absorption in the animal *** the current study,we aimed to explore the effects of PEITC on DON-induced apoptosis,intestinal tight junction disorder,and its potential molecular mechanism in the porcine jejunum epithelial cell line(IPEC-J2).Our results indicated that PEITC treatment markedly alleviated DON-induced cytotoxicity,decreasing the apoptotic cell percentage and pro-apoptotic mRNA/protein levels,and increasing zonula occludens-1(ZO-1),occludin and claudin-1 mRNA/protein ***,PEITC treatment ameliorated DON-induced an increase of the inducible nitric oxide synthase(iNOS)and cyclooxygenase 2(COX-2)mRNA levels and intracellular reactive oxygen species(ROS)level,and a decrease of glutathione peroxidase 1(GPx1),superoxide dismutase 2(SOD2),catalase(CAT)and heme oxygenase 1(HO-1)mRNA ***,PEITC treatment significantly down-regulated autophagy-related protein 5(ATG5),beclin-1 and microtubuleassociated protein 1 light chain 3B(LC3-II)mRNA/protein levels,decreased the number of green fluorescent protein-microtubule-associated protein 1 light-chain 3(GFP-LC3)puncta and phosphatidylinositol 3 kinase(PI3K)protein expression,and up-regulated phospho-protein kinase B(p-Akt)and phospho-mammalian target of rapamycin(p-mTOR)protein expression against ***,the activation of autophagy by rapamycin,an autophagy agonist,abolished the protective effects of PEITC against DON-induced cytotoxicity,apoptosis and intestinal tight junction ***,PEITC could confer protection against DON-induced porcine intestinal epithelial cell injury by suppressing ROSmediated autophagy.

关键词： Deoxynivalenol Phenethyl isothiocyanate Autophagy Oxidative damage Porcine intestinal epithelial cell

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The protective effects of selenium-enriched probiotics on carbon tetrachloride-induced liver fibrosis in rats Short title:Effects of selenium-enriched probiotics on rat liver fibrosis

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畜牧与饲料科学 2015年第Z1期 335-335页

作者： LIU Yunhuan LIU Qing YE Gengping ALAMZEB Khan LIU Jin GAN Fang ZHANG Xian SHAHNAWAZ Kumbhar HUANG Kehe institute of nutritional and metabolic disorders in domestic animals and fowls Nanjing Agricultural University

This study aimed to investigate the effects of Se-enriched probiotics（SP） on the liver fibrosis induced by CCI4 in *** results showed that SP significantly decreased serum alanine aminotransferase（87.0±1.96） U/L,aspartate aminotransferase（101 ±3.13） U/L,hepatic hydroxyproline（898 ±72.5） μg/g and malondialdehyde（2.39±0.34） nmol/mg levels,but increased glutathione peroxidase（37.2 ± 3.19） U/mg,superoxide dismutase（201± 19.2） U/mg and glutathione levels（3.32±0.25） mg/g（P<0.05） in rats treated by *** suppressed hepatic inflammation and necrosis induced by ***,SP significantly reduced the expression of a-smooth muscle actin,collagen,TGF-β1,TIMP-1 and inflammation-related gene and induced apoptosis of activated hepatic stellate cells（P<0.05） in rats treated by *** results suggest that SP could protect the liver fibrosis by attenuating hepatic oxidative stress,suppressing hepatic inflammation and inducing apoptosis of hepatic stellate cells.

关键词： liver fibrosis selenium-enriched probiotics(SP) oxidative stress inflammation apoptosis

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Organic selenium alleviated the formation of ethylene glycol-induced calcium oxalate renal calculi by improving osteopontin expression and antioxidant capability in dogs

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畜牧与饲料科学 2015年第Z1期 335-335页

作者： LIU Yongwang XU Haibin ZHONG Wenting SHEN Qingpeng ZHUANG Tenghan HUANG Kehe institute of nutritional and metabolic disorders in domestic animals and fowls Nanjing Agricultural University

Twenty one-year-old local male dogs were randomly assigned into four groups(five dogs per group).The control and the ethylene glycol(EG) groups were fed basal diets without and with EG,and the EG+sodium selenite(EG+SS) and EG+selenium yeast(EG+SY) groups were fed basal diets with EG containing SS and SY,***,urine and renal samples were taken after 18 weeks of *** results showed that compared with the control group,the serum calcium levels and antioxidase activities significantly decreased in the EG *** creatinine,urea nitrogen and malondialdehyde(MDA) levels and urine calcium and oxalate levels significantly *** oxalate crystal deposition and osteopontin(OPN) mRNA and protein expression in the renal tissues significantly *** changes above in the EG group were reversed within limits by adding selenium in the diets(both EG+SS and EG+SY groups).Further,compared with the EG+SS group,the EG+SY group showed better effects in decreasing the formation of EG-induced calcium oxalate renal calculi and OPN expression and improving antioxidant capability in *** indicates that organic selenium has the potential value to alleviate the formation of EG-induced calcium oxalate renal calculi.

关键词： selenium calcium oxalate renal calculi OPN antioxidation

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