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B7-H1 expression is associated with expansion of regulatory T cells in colorectal carcinoma

World Journal of Gastroenterology 2012年第9期18卷 971-978页

作者： Dong Hua Jing Sun Yong Mao Lu-Jun Chen Yu-Yu Wu Xue-Guang Zhang Department of Oncology the FourthAffiliated Hospital of Suzhou University Wuxi 214062 JiangsuProvince China institute of medicalbiotechnology Soochow University Suzhou 215007 Jiangsu Pro-vince China Clinical central Laboratory The Third AffiliatedHospital of Soochow University Changzhou 213003 JiangsuProvince China Department of Pathology The Fourth Affiliated Hos-pital of Soochow University Wuxi 214062 Jiangsu ProvinceChina

AIM:To investigate the expression of B7-H1 in human colorectal carcinoma (CRC) to define its regulating effects on T cells in tumor ***:One hundred and two paraffin blocks and 33 fresh samples of CRC tissues were subject to this *** was performed for B7-H1 and CD3 staining in CRC ***-Hypaque density gradient centrifugation was used to isolate peripheral blood mononuclear cells of fresh CRC tissues;flow cytometry and immunofluorescence staining were used for detection of regulatory T *** was analyzed with statistical ***:Costimulatory molecule B7-H1 was found strongly expressed in CRC tissues,localized in tumor cell membrane and cytoplasm,while weak or none expression of B7-H1 was detected in pared normal colorectal ***,CD3 positive T cells were found congregated in CRC tumor nest and *** analysis showed that B7-H1 expression level was negatively correlated to the total T cell density in tumor nest (P<0.0001) and tumor stroma (P=0.0200) of 102 cases of CRC *** the total T cells,a variable amount of regulatory T cells with a clear Foxp3+ (forkhead box P3) staining could be detected in CRC tissues and patients' ***,in the 33 samples (15 cases of B7-H1 high CRC tissues and 18 cases of B7H1 low CRC tissues) of freshly isolated mononuclear cells from CRC tissues,the percentages of CD4+ Foxp3+ and CD8+ Foxp3+ regulatory T cells were found remarkably higher in B7-H1 high CRC tissues than in B7-H1 low CRC tissues (P=0.0024,P=0.0182),indicating that B7-H1 expression was involved in proliferation of regulatory T *** significant difference was found in CRC peripheral blood (P=0.0863,P=0.0678).PD-1 is the specific ligand for B7-H1 pathway transferring inhibitory signal to T cell,which is expressed by activated T *** further analysis of PD-1 expression on T cells in CRC tissues showed that conventional T cells (CD4+ Foxp3/CD8+ Foxp3),which was t

关键词：调节性T细胞大肠癌调节效应结直肠癌微环境 CRC 肿瘤

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