检索结果-南通市图书馆

Dominant changes in the breast muscle lipid profiles of broiler chickens with wooden breast syndrome revealed by lipidomics analyses

在线全文

学校读者我要写书评

暂无评论

Journal of animal Science and Biotechnology 2022年第6期13卷 1703-1713页

作者： Ranran Liu Fuli Kong Siyuan Xing Zhengxiao He Lu Bai Jiahong Sun Xiaodong Tan Di Zhao Guiping Zhao Jie Wen institute of animal sciences Chinese Academy of Agricultural SciencesState Key Laboratory of Animal NutritionKey Laboratory of Animal(Poultry)Genetics Breeding and ReproductionMinistry of AgricultureBeijingChina

Background: Chicken is the most consumed meat worldwide and the industry has been facing challenging myopathies. Wooden breast(WB), which is often accompanied by white striping(WS), is a serious myopathy adversely affecting meat quality of breast muscles. The underlying lipid metabolic mechanism of WB affected broilers is not fully understood.Results: A total of 150 chickens of a white-feathered, fast-growing pure line were raised and used for the selection of WB, WB + WS and control chickens. The lipids of the breast muscle, liver, and serum from different chickens were extracted and measured using ultra performance liquid chromatography(UPLC) plus Q-Exactive Orbitrap tandem mass spectrometry. In the breast, 560 lipid molecules were identified. Compared to controls, 225/225 of 560 lipid molecules(40.2%) were identified with differential abundance(DA), including 92/100 significantly increased neutral lipids and 107/98 decreased phospholipids in the WB/WB + WS groups, respectively. The content of monounsaturated fatty acids(MUFA) was significantly higher, and the polyunsaturated fatty acids(PUFA) and saturated fatty acids(SFA) were significantly lower in the affected breasts. In the liver, 434 lipid molecules were identified, and 39/61 DA lipid molecules(6.7%/14.1%) were detected in the WB and WB + WS groups, respectively. In the serum, a total of 529 lipid molecules were identified and 4/44 DA lipid molecules(0.8%/8.3%) were detected in WB and WB + WS group, respectively. Compared to controls, the content of MUFAs in the serum and breast of the WB + WS group were both significantly increased, and the content of SFAs in two tissues were both significantly decreased. Only five lipid molecules were consistently increased in both liver and serum in WB + WS group.Conclusions: We have found for the first time that the dominant lipid profile alterations occurred in the affected breast muscle. The relative abundance of 40.2% of lipid molecules were changed and is characteristic of increased neutral lipids and decreased p

关键词： Broiler chicken Lipid profle Liver Serum Wooden breast

Time-course effects of different fiber-rich ingredients on energy values,microbiota composition and SCFA profile in growing pigs

在线全文

学校读者我要写书评

暂无评论

animal Nutrition 2023年第1期12卷 263-275页

作者： Qingtao Gao Zhengqun Liu Kai Li Guosong Bai Lei Liu Ruqing Zhong Liang Chen Hongfu Zhang State Key laboratory of animal Nutrition Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193China institute of animal Science and Veterinary Tianjin Academy of Agriculture SciencesTianjinChina

This study was to investigate time-course effects of different types of dietary fiber on the energy values,fecal microbiota and short-chain fatty acid(SCFA)concentration in growing pigs.A total of 24 barrows(initial body weight,19.8±0.5 kg)were assigned to 4 dietary treatments based on body weight(BW)in a completely randomized design,including a basal diet(CON)and 3 fiber-rich diets replacing corn,soybean meal and soybean oil in the CON diet with 20%sugar beet pulp(SBP),defatted rice bran(DFRB)or soybean hull(SBH),respectively.Fresh feces were sampled on d 7,14 and 21,followed by 5 d total feces and urine collections.The results showed that there were no differences in DE and ME between any of the fiber ingredients on d 7,14 or 21.However,fiber inclusion decreased the DE and ME of the diet(P<0.05)regardless of the time effect.Principal coordinate analysis(PCoA)revealed distinctly different microbial communities on the DFRB diet and SBH diet across different times(P<0.05)and the fecal microbiota of the 4 diet groups demonstrated notably distinct clusters at each time point(P<0.05).With adaptation time increased from 7 to 21 d,cellulose-degrading bacteria and SCFA-producing bacteria(e.g.,Ruminococcaceae_UCG-014,Rikenellaceae_RC9_gut_group and Bifidobacterium)increased in the fiber inclusion diets,and pathogenic genera(e.g.,Streptococcus and Selenomonas)were increased in the basal diet(P<0.05).Furthermore,the gut microbiota of growing pigs adapted more easily and quickly to the SBP diet compared to the DFRB diet,as reflected by the concentration of propionate,butyrate,isovalerate and total SCFA which increased with time for growing pigs fed the DFRB diet(P<0.05).Collectively,our results indicated at least 7 d adaptation was required to evaluate the energy values of fiber-rich ingredients,as the hindgut microbiota of growing pigs may need more time to adapt to a high fiber diet,especially for insoluble dietary fiber.

关键词： Adaptation time Energy value Fecal microbiota Fiber-rich ingredient Growing pig

Plasma metabolic profiling reveals that chromium yeast alleviates the negative effects of heat stress in mid-lactation dairy cows

在线全文

学校读者我要写书评

暂无评论

animal Nutrition 2023年第2期13卷 401-410页

作者： Yeqianli Wo Fengtao Ma Qiang Shan Duo Gao Yuhang Jin Peng Sun State Key laboratory of animal Nutrition Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing100193China

Chromium yeast(CY)supplementation has the potential to alleviate the negative effects of heat stress in dairy cows,but the mechanism remains elusive.We aimed to identify the metabolic mechanisms whereby CY supplementation alleviates the negative effects of heat stress in mid-lactation dairy cows.Twelve Holstein dairy cows with similar milk yield(24.6±1.5 kg/d),parity(2 or 3)and days in milk(125±8 d)were fed the same basal diet containing 0.09 mg of Cr/kg DM.They were allocated randomly to 2 groups:a control group(CON,without CY supplementation)and a CY group(CY,administered 0.36 mg Cr/kg DM).The experiment was performed over 8 weeks during a hot summer,in which the mean temperature-humidity index was 79.0±3.13(>72),indicating that the dairy cows were exposed to heat stress.Chromium yeast supplementation reduced rectal temperature(P=0.032),and increased the lactation performance by increasing the yield of milk(+2.6 kg/d),protein,lactose and total solid,and protein and lactose percentages in the milk of the heat-stressed dairy cows(P1.0,P<0.05),and found to be involved in 6 Kyoto Encyclopedia of Genes and Genomes pathways,including those involved in nicotinate and nicotinamide metabolism.Specifically,plasma concentration of nicotinamide was higher after CY supplementation,which might also contribute to the reduction of rectal temperature,the regulation of glucose homeostasis,and an improvement in the lactation performance of heat-stressed dairy cows.In conclusion,CY supplementation reduces rectal temperature,influences metabolism by reduci

关键词： Chromium yeast Heat stress Metabolomics Nicotinamide Dairy cow

Integrated liver proteomics and metabolomics identify metabolic pathways affected by pantothenic acid deficiency in Pekin ducks

在线全文

学校读者我要写书评

暂无评论

animal Nutrition 2022年第4期11卷 1-14页

作者： Jing Tang Yongbao Wu Bo Zhang Suyun Liang Zhanbao Guo Jian Hu Zhengkui Zhou Ming Xie Shuisheng Hou State Key laboratory of animal Nutrition Key Laboratory of Animal(Poultry)Genetics Breeding and ReproductionMinistry of Agriculture and Rural AffairsInstitute of Animal SciencesChinese Academy of Agricultural Sciences100193BeijingChina

Pantothenic acid deficiency(PAD)in animals causes growth depression,fasting hypoglycemia and impaired lipid and glucose metabolism.However,a systematic multi-omics analysis of effects of PAD on hepatic function has apparently not been reported.We investigated liver proteome and metabolome changes induced by PAD to explain its effects on growth and liver metabolic disorders.Pekin ducks(1-dold,n=128)were allocated into 2 groups,with 8 replicates and 8 birds per replicate.For 16 d,all ducks had ad libitum access to either a PAD or a pantothenic acid adequate(control,CON)diet,formulated by supplementing a basal diet with 0 or 8 mg pantothenic acid/kg of diet,respectively.Liver enlargement,elevated liver glycogen concentrations and decreased liver concentrations of triglyceride and unsaturated fatty acids were present in the PAD group compared to the CON group.Based on integrated liver proteomics and metabolomics,PAD mainly affected glycogen synthesis and degradation,glycolysis and gluconeogenesis,tricarboxylic acid(TCA)cycle,peroxisome proliferator-activated receptor(PPAR)signaling pathway,fatty acid beta oxidation,and oxidative phosphorylation.Selected proteins were confirmed by Western blotting.Downregulation of proteins and metabolites involved in glycogen synthesis and degradation,glycolysis and gluconeogenesis implied that these processes were impaired in PAD ducks,which could have contributed to fasting hypoglycemia,liver glycogen storage,insufficient ATP production,and growth retardation.In contrast,PAD also upregulated proteins and metabolites involved in fatty acid beta oxidation,the TCA cycle,and oxidative phosphorylation processes in the liver;presumably compensatory responses to produce ATP.We inferred that PAD decreased liver triglyceride and unsaturated fatty acids by activating fatty acid beta oxidation and impairing unsaturated fatty acid synthesis.These findings contributed to our understanding of the mechanisms of PAD-induced changes in hepatic metabolism.

关键词： Pantothenic acid Hepatic metabolism Proteomics Metabolomics Hypoglycemia

Supplementation with galacto-oligosaccharides in early life persistently facilitates the microbial colonization of the rumen and promotes growth of preweaning Holstein dairy calves

在线全文

学校读者我要写书评

暂无评论

animal Nutrition 2022年第3期10卷 223-233页

作者： Meinan Chang Feifei Wang Fengtao Ma Yuhang Jin Peng Sun State Key laboratory of animal Nutrition Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing100193China

We aimed to determine the effects of dietary supplementation with galacto-oligosaccharides(GOS)on the growth performance,serum parameters,and the rumen microbial colonization and fermentation of pre-weaning dairy calves.The study comprised 2 phases of 28 and 42 d,respectively.During phase 1,24 newborn female Holstein dairy calves were randomly allocated to consume a diet supplemented with 10 g/d GOS(GOS,n=12)or not(CON,n=12).Thereafter,during phase 2,the GOS group was further divided into 2 groups:one that continued to consume GOS(GOSC,n=6)and one that no longer consumed GOS(GOSS,n=6),alongside the CON group.Galacto-oligosaccharides increased the average daily gain(ADG),body weight,feed efficiency,and serum high-density lipoprotein-cholesterol concentration of dairy calves during phase 1(P<0.05).Supplementation with GOS for the entire study reduced the incidence of diarrhea and increased the serum total protein and Ca concentrations(P<0.05)compared with the CON group.The effect of GOS supplementation persisted after it was stopped because the ADG and final body weight of the GOSS group were higher than those of the CON group(P<0.05).Furthermore,the GOSS group showed a persistently lower incidence of diarrhea and greater colonization of the rumen with probiotics,at the expense of less beneficial bacteria,which would promote ruminal fermentation and microbial protein synthesis.These findings provide a theoretical basis for the rational application of prebiotics and have important practical implications for the design of early life dietary interventions in dairy calf rearing.

关键词： Galacto-oligosaccharide Dairy calf Growth performance Rumen microbiota

Sirtuin 3 regulation:a target to alleviateβ-hydroxybutyric acid-induced mitochondrial dysfunction in bovine granulosa cells

在线全文

学校读者我要写书评

暂无评论

Journal of animal Science and Biotechnology 2023年第4期14卷 1377-1394页

作者： Shanjiang Zhao Jianfei Gong Yi Wang Nuo Heng Huan Wang Zhihui Hu Haoyu Wang Haobo Zhang Huabin Zhu State Key laboratory of animal Nutrition Key Laboratory of Animal GeneticsBreeding and Reproduction of Ministry of Agriculture and Rural AffairsInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijingChina

Background During the transition period,the insufficient dry matter intake and a sharply increased in energy consumption to produce large quantities of milk,high yielding cows would enter a negative energy balance(NEB)that causes an increase in ketone bodies(KBs)and decrease in reproduction efficiency.The excess concentrations of circulating KBs,represented byβ-hydroxybutyric acid(BHBA),could lead to oxidative damage,which potentially cause injury to follicular granulosa cells(fGCs)and delayed follicular development.Sirtuin 3(Sirt3)regulates mitochondria reactive oxygen species(mitoROS)homeostasis in a beneficial manner;however,the molecular mechanisms underlying its involvement in the BHBA-induced injury of fGCs is poorly understood.The aim of this study was to explore the protection effects and underlying mechanisms of Sirt3 against BHBA overload-induced damage of fGCs.Results Our findings demonstrated that 2.4 mmol/L of BHBA stress increased the levels of mitoROS in bovine fGCs.Further investigations identified the subsequent mitochondrial dysfunction,including an increased abnormal rate of mitochondrial architecture,mitochondrial permeability transition pore(MPTP)opening,reductions in mitochondrial membrane potential(MMP)and Ca^(2+)release;these dysfunctions then triggered the caspase cascade reaction of apoptosis in fGCs.Notably,the overexpression of Sirt3 prior to treatment enhanced mitochondrial autophagy by increasing the expression levels of Beclin-1,thus preventing BHBA-induced mitochondrial oxidative stress and mitochondrial dysfunction in fGCs.Furthermore,our data suggested that the AMPK-mTOR-Beclin-1 pathway may be involved in the protective mechanism of Sirt3 against cellular injury triggered by BHBA stimulation.Conclusions These findings indicate that Sirt3 protects fGCs from BHBA-triggered injury by enhancing autophagy,attenuating oxidative stress and mitochondrial damage.This study provides new strategies to mitigate the fGCs injury caused by excessive BHBA stress in dairy cows with ketosis.

关键词： BHBA Dairy cows Granulosa cells Ketosis Mitochondrial function Sirt3

Ruminal bacterial community is associated with the variations of total milk solid content in Holstein lactating cows

在线全文

学校读者我要写书评

暂无评论

animal Nutrition 2022年第2期9卷 175-183页

作者： Kaizhen Liu Yangdong Zhang Guoxin Huang Nan Zheng Shengguo Zhao Jiaqi Wang State Key laboratory of animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China Key laboratory of Quality&Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China

Total milk solid(TMS)content directly reflects the quality of milk.Rumen bacteria ferment dietary components,the process of which generates the precursors for the synthesis of milk solid,therefore,the variation in rumen bacterial community could be associated with milk solid in dairy cows.In this study,45 healthy mid-lactation Holstein dairy cows with the similar body weight,lactation stage,and milk yield were initially used for the selection of 10 cows with high TMS(HS)and 10 cows with low TMS(LS).All those animals were under the same feeding management,and the individual milk yield was recorded for 14 consecutive days before milk and rumen fluid were sampled.Rumen fluid was used to determine bacterial community by 16S rRNA gene sequencing technique.The HS cows had significantly greater feed intake and milk TMS,fat,protein content than LS cows(P0.05),nor was the acetate-to-propionate ratio,pH value,ammonia nitrogen and microbial crude protein concentrations(P>0.05).Significant differences in the relative abundances of some bacterial genera were found between HS and LS cows.Spearman’s rank correlation analysis revealed that TMS content was correlated positively with the abundances of Ruminococcaceae UCG-014,Ruminococcaceae NK4A214 group,Prevotellaceae UCG-001,Butyrivibrio 2,Prevotellaceae UCG-003,Candidatus Saccharimonas,Ruminococcus 2,Lachnospiraceae XPB1014 group,probable genus 10,Eubacterium ventriosum group,but negatively correlated with Pyramidobacte.In addition,Ruminococcaceae UCG-014,Ruminococcus 2,Ruminococcaceae UCG001,probable genus 10 and Eubacterium ventriosum group might boost the total VFA production in the rumen.In conclusion,the dry matter intake of dairy cows and some special bacteria in rumen were significantly associated w

关键词： Dairy cow Total milk solid content Rumen bacteria Milk fat

Feedomics provides bidirectional omics strategies between genetics and nutrition for improved production in cattle

在线全文

学校读者我要写书评

暂无评论

animal Nutrition 2022年第2期9卷 314-319页

作者： Xiaohan Liu Yifan Tang Jiajin Wu Jian-Xin Liu Hui-Zeng Sun institute of Dairy Science MoE Key Laboratory of Molecular Animal NutritionCollege of Animal SciencesZhejiang UniversityHangzhouChina

Increasing the efficiency and sustainability of cattle production is an effective way to produce valuable animal proteins for a growing human population.Genetics and nutrition are the 2 major research topics in selecting cattle with beneficial phenotypes and developing genetic potentials for improved performance.There is an inextricable link between genetics and nutrition,which urgently requires researchers to uncover the underlying molecular mechanisms to optimize cattle production.Feedomics integrates a range of omic techniques to reveal the mechanisms at different molecular levels related to animal production and health,which can provide novel insights into the relationships of genes and nutrition/nutrients.In this review,we summarized the applications of feedomics techniques to reveal the effect of genetic elements on the response to nutrition and investigate how nutrients affect the functional genome of cattle from the perspective of both nutrigenetics and nutrigenomics.We highlighted the roles of rumen microbiome in the interactions between host genes and nutrition.Herein,we discuss the importance of feedomics in cattle nutrition research,with a view to ensure that cattle exhibit the best production traits for human consumption from both genetic and nutritional aspects.

关键词： Cattle Feedomics Nutrigenetics Nutrigenomics Rumen microbiome Production

Blood neutrophil extracellular traps:a novel target for the assessment of mammary health in transition dairy cows

在线全文

学校读者我要写书评

暂无评论

Journal of animal Science and Biotechnology 2023年第3期14卷 1068-1079页

作者： Luyi Jiang Huizeng Sun Fengfei Gu Jin He Fengqi Zhao Jianxin Liu institute of Dairy Science College of Animal SciencesZhejiang UniversityHangzhouChina Ministry of Education Key laboratory of Molecular animal Nutrition Zhejiang UniversityHangzhouChina institute of animal Genetics and Reproduction College of Animal SciencesZhejiang UniversityHangzhouChina Department of animal&Veterinary sciences University of VermontBurlingtonUSA

Background:Mammary health is important for transition dairy cows and has been well recognized to exert decisive effects on animal welfare.However,the factors influencing mammary health are still unclear.Differential somatic cell count(DSCC)could reflect the mastitis risk since it is the percentage of neutrophils plus lymphocytes in total somatic cells and could be reflective of mammary health of dairy cows.This work aimed to investigate the assessment and prognosis of the health of transition cows based on blood neutrophil extracellular traps(NETs).Results:Eighty-four transition Holstein dairy cows were selected.The serum was sampled in all the animals at week 1 pre-and postpartum,and milk was sampled at week 1 postpartum.Based on the DSCC in milk at week 1,cows with lower(7.4%±4.07%,n=15)and higher(83.3%±1.21%,n=15)DSCCs were selected.High DSCC cows had higher levels of red blood cell counts(P<0.05),hemoglobin(P=0.07),and hematocrit(P=0.05),higher concentrations of serum oxidative variables[reactive oxygen species(P<0.05),malondialdehyde(P<0.05),protein carbonyl(P<0.05),and 8-hydroxy-2-deoxyguanosine(P=0.07)],higher levels of serum and milk NETs(P<0.05)and blood-milk barrier indicators,including serumβ-casein(P=0.05)and milk immunoglobulin G2(P=0.09),than those of low DSCC cows.In addition,lower concentrations of serum nutrient metabolites(cholesterol and albumin)(P<0.05)and a lower level of serum deoxyribonuclease I(P=0.09)were observed in high DSCC cows than in low DSCC cows.Among the assessments performed using levels of the three prepartum serum parameters(NETs,deoxyribonuclease I andβ-casein),the area under the curve(0.973)of NETs was the highest.In addition,the sensitivity(1.00)and specificity(0.93)were observed for the discrimination of these cows using NETs levels with a critical value of 32.2 ng/mL(P<0.05).Conclusions:The formation of NETs in blood in transition dairy cows may damage the integrity of the blood-milk barrier and thereby increase the risk for mastitis in postpartum cows.

关键词： Blood-milk barrier Differential somatic cell count Mastitis risk Neutrophil extracellular traps