检索结果-南通市图书馆

Gut microbial metabolite targets HDAC3-FOXK1-interferon axis in fibroblast-like synoviocytes to ameliorate rheumatoid arthritis

引用

bone Research 2024年第2期12卷 421-437页

作者： Hongzhen Chen Xuekun Fu Xiaohao Wu Junyi Zhao Fang Qiu Zhenghong Wang Zhuqian Wang Xinxin Chen Duoli Xie Jie Huang Junyu Fan Xu Yang Yi Song Jie Li Dongyi He Guozhi Xiao Aiping Lu Chao Liang Department of Systems Biology School of Life SciencesSouthern University of Science and TechnologyGuangdong Provincial Key Laboratory of Cell Microenvironment and Disease ResearchShenzhen Key Laboratory of Cell MicroenvironmentShenzhen 518055China institute of Integrated Bioinfomedicine and Translational Science(IBTS) School of Chinese MedicineHong Kong Baptist UniversityHong Kong SAR 999077China Department of Biochemistry School of MedicineSouthern University of Science and TechnologyGuangdong Provincial Key Laboratory of Cell Microenvironment and Disease ResearchShenzhen Key Laboratory of Cell MicroenvironmentShenzhen 518055China Division of immunology and Rheumatology Stanford UniversityStanfordCA 94305USA VA Palo Alto Health Care System Palo AltoCA 94304USA institute of Plant and Food Science Department of BiologySouthern University of Science and TechnologyShenzhen 518055China Department of Rheumatology Guanghua Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai University of Traditional Chinese MedicineShanghaiChina Department of Computational Biology St.Jude Children’s Research HospitalMemphisTNUSA Department of Laboratory Medicine Peking University Shenzhen HospitalShenzhenChina Guangdong-Hong Kong-Macao Joint Lab on Chinese Medicine and Immune Disease Research Guangzhou 510006China Shanghai University of Traditional Chinese Medicine Shanghai 200032China State Key Laboratory of Proteomics National Center for Protein Sciences(Beijing)Beijing Institute of Lifeomics100850 BeijingChina

Rheumatoid arthritis(RA)is an autoimmune *** studies hold an opinion that gut microbiota is environmentally acquired and associated with RA ***,accumulating evidence demonstrates that genetics also shape the gut *** is known that some strains of inbred laboratory mice are highly susceptible to collagen-induced arthritis(CIA),while the others are resistant to ***,we show that transplantation of fecal microbiota of CIA-resistant C57BL/6J mice to CIA-susceptible DBA/1J mice confer CIA resistance in DBA/1J mice.C57BL/6J mice and healthy human individuals have enriched *** than DBA/1J mice and RA *** of *** prevents CIA in DBA/1J *** identify that *** mainly produces propionate and C57BL/6J mice and healthy human individuals have higher level of ***-like synoviocytes(FLSs)in RA are activated to undergo tumor-like *** disrupts HDAC3-FOXK1 interaction to increase acetylation of FOXK1,resulting in reduced FOXK1 stability,blocked interferon signaling and deactivation of *** treat CIA mice with propionate and show that propionate attenuates ***,a combination of propionate with anti-TNF etanercept synergistically relieves *** results suggest that *** or propionate could be an alternative or complementary approach to the current therapies.

关键词： HDAC3 cytes interferon

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of CXCR3/HO-1 genes modified bone marrow mesenchymal stem cells on small bowel transplant rejection

引用

World Journal of Gastroenterology 2017年第22期23卷 4016-4038页

作者： Ming-Li Yin Hong-Li Song Yang Yang Wei-Ping Zheng Tao Liu Zhong-Yang Shen Tianjin first Central hospital Clinic institute Tianjin Medical University Department of Organ transplantation Tianjin First Central HospitalTianjin Clinical Research Center for Organ Transplantation and Tianjin Key Laboratory of Organ Transplantation Department of Organ transplantation Tianjin First Central Hospital Tianjin first Central hospital and Key Laboratory of Emergency and Care Medicine of Ministry of Health

AIM To investigate whether bone marrow mesenchymal stem cells (BMMSCs) modified with the HO-1 and CXCR3 genes can augment the inhibitory effect of BMMSCs on small bowel transplant rejection. METHODS Lewis rat BMMSCs were cultured in vitro. Third-passage BMMSCs were transduced with the CXCR3 / HO-1 genes or the HO-1 gene alone. The rats were divided into six groups and rats in the experimental group were pretreated with BMMSCs 7 d prior to small bowel transplant. Six time points (instant, 1 d, 3 d, 7 d, 10 d, and 14 d) (n = 6) were chosen for each group. Hematoxylin-eosin staining was used to observe pathologic rejection, while immunohistochemistry and Western blot were used to detect protein expression. Flow cytometry was used to detect T lymphocytes and enzyme linked immunosorbent assay was used to detect cytokines. RESULTS The median survival time of BMMSCs from the CXCR3/HO-1 modified group (53 d) was significantly longer than that of the HO-1 modified BMMSCs group (39 d), the BMMSCs group (26 d), and the NS group (control group) (16 d) (P < 0.05). Compared with BMMSCs from the HO-1 modified BMMSCs, BMMSCs, and NS groups, rejection of the small bowel in the CXCR3 / HO-1 modified group was significantly reduced, while the weight of transplant recipients was also significantly decreased (P < 0.05). Furthermore, IL-2, IL-6, IL-17, IFN-gamma, and TNF-alpha levels were significantly decreased and the levels of IL-10 and TGF-beta were significantly increased (P < 0.05). CONCLUSION BMMSCs modified with the CXCR3 and HO-1 genes can abrogate the rejection of transplanted small bowel more effectively and significantly increase the survival time of rats that receive a small bowel transplant.

关键词： bone marrow mesenchymal stem cells CXCR3 HO-1 Small bowel transplantation Rejection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rapamycin combined with allogenic immature dendritic cells selectively expands CD4^+CD25^+Foxp3^+ regulatory T cells in rats

引用

Hepatobiliary & Pancreatic Diseases International 2012年第2期11卷 203-208页

作者： Guo-Ying Wang Qi Zhang Yang Yang Wen-Jie Chen Wei Liu Nan Jiang Gui-Hua Chen chgh1955@*** Liver transplantation center Third Affiliated Hospital Transplantation Research Institute Sun Yat-Sen University Organ Transplantation Research Center of Guangdong Province Guangzhou 510630 China

ReferencesReferencesBackgroundDendritic cells (DCs) can initiate the expansion of regulatory T cells (Tregs), which play an indispensable role in inducing transplantation tolerance. Some studies have investigated the effect of the immunosuppressant rapamycin (Rapa) on Tregs in vitro. However, the in vivo effect of Rapa combined with immature DCs (iDCs) on Tregs is unknown. This study was undertaken to determine whether allogenic iDCs combined with a short course of Rapa have the ability to selectively expand the CD4+CD25+Foxp3+ Tregs in a rat *** Norway rats were injected intravenously with 2×106 Lewis iDCs followed by 1 mg/kg per day Rapa intraperitoneally for 7 consecutive days. On day 8, the levels of CD4+CD25+Foxp3+ Treg cells in peripheral blood and spleen cells were analyzed by flow cytometry. IL-2, IL-4, TGF-β1, and IFN-γ levels in serum were assessed by ELISA. The experimental animals were divided into four groups: control, Rapa-treated, iDC-treated, and ***4+CD25+Foxp3+ Tregs comprised 7%-8%of CD4+ T cells in control rats. Rapa combined with iDCs enhanced this percentage in the peripheral blood and spleen. However, the levels of Tregs did not significantly change after treatment with Rapa or iDCs alone. The levels of CD4+CD25?/sup>Foxp3+ T cells and CD4+ CD25+Foxp3?/sup> T cells in CD4+ T cells did not significantly change in the combined group. The TGF-β1 level in serum from the combined group increased significantly compared with the other *** significantly higher percentage of CD4+ CD25+ Foxp3+ Tregs was found in rats treated with allogenic iDCs and a short course of Rapa, along with an increase in the TGF-β1 level in serum. This improved protocol may be a promising therapeutic strategy to increase Tregs, which are beneficial to the induction of peritransplant tolerance.

关键词： dendritic cells regulatory T cells rapamycin

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

人类肝移植图谱揭示了一种与早期移植物功能不全发生相关的致病免疫生态位（英文）

引用

Engineering 2024年第5期 193-208页

作者：邵鑫王峥汪恺陆晓燕张萍郭蓉芳廖杰杨鹏挥郑树森徐骁范骁辉 Pharmaceutical Informatics institute College of Pharmaceutical Sciences Zhejiang University National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River Delta Zhejiang University Zhejiang University School of Medicine NHC Key Laboratory of Combined Multi-organ transplantation Department of Hepatobiliary and Pancreatic Surgery Shulan (Hangzhou) Hospital Affiliated to Shulan International Medical College Zhejiang Shuren University Engineering Research center of Innovative Anticancer Drugs Ministry of Education

Liver transplantation（LT） is the standard therapy for individuals afflicted with end-stage liver *** notable advancements in LT technology, the incidence of early allograft dysfunction（EAD）remains a critical concern, exacerbating the current organ shortage and detrimentally affecting the prognosis of recipients. Unfortunately, the perplexing hepatic heterogeneity has impeded characterization of the cellular traits and molecular events that contribute to EAD. Herein, we constructed a pioneering single-cell transcriptomic landscape of human transplanted livers derived from non-EAD and EAD patients, with 12 liver samples collected from 7 donors during the cold perfusion and portal reperfusion stages. Comparison of the 75 231 cells of non-EAD and EAD patients revealed an EAD-associated immune niche comprising mucosal-associated invariant T cells, granzyme B+（GZMB+） granzyme K+（GZMK+） natural killer cells, and S100 calcium binding protein A12+（S100A12+） neutrophils. Moreover, we verified this immune niche and its association with EAD occurrence in two independent cohorts. Our findings elucidate the cellular characteristics of transplanted livers and the EAD-associated pathogenic immune niche at the single-cell level, thus, offering valuable insights into EAD onset.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The DDB1-DCAF2 complex is essential for B cell development because it regulates cell cycle progression

引用

Cellular & Molecular immunology 2021年第3期18卷 758-760页

作者： Zhonghui Xue Jing Guo Ruoyu Ma Lina Zhou Yixin Guo Yong Cang Hengyu Fan Jian Chen Wenbin Qian Lie Wang bone marrow transplantation center of the first affiliated hospital and Institute of ImmunologyZhejiang University School of MedicineHangzhouChina lnstitute of Hematology Zhejiang University and Zhejiang Engineering Laboratory for Stem Cell and ImmunotherapyHangzhouChina Research center of Clinical Medicine Affiliated Hospital of Nantong UniversityNantongJiangsuChina School of Life Science and Technology ShanghaiTech UniversityShanghaiChina MOE Key Laboratory for Biosystems Homeostasis and Protection and Innovation center for Cell Signaling Network Life Sciences InstituteZhejiang UniversityHangzhouChina Department of General Surgery The Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouChina Laboratory Animal center Zhejiang UniversityHangzhouChina

B cell development in the bone marrow is critical for producing numerous B cells that play an essential role in the adaptive immune response.B cells develop from common lymphoid progenitors(CLPs),which then differentiate through a series of stages,which include prepro-B cells,pro-B cells,pre-B cells,immature B cells,and mature B cells.

关键词： lymphoid progenitor cycle

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Exploring the impact of rotavirus vaccination on antibiotic prescription and resistance:A comprehensive systematic review

引用

World Journal of Virology 2024年第2期13卷 115-122页

作者： Lakshmi Venkata Simhachalam Kutikuppala Matei-Alexandru Cozma Gautam Maddineni Harshal Prakash Chorya Nayanika Tummala Swathi Godugu Jyothi Swaroop Chintala Mihnea-Alexandru Găman Department of General Surgery Dr NTR University of Health SciencesVijayawada 520008Andhra PradeshIndia Faculty of Medicine “Carol Davila”University of Medicine and PharmacyBucharest 050474Romania Department of Gastroenterology Colentina Clinical HospitalBucharest 020125Romania Department of Internal Medicine Florida State UniversityCape CoralFL 33909United States Department of Internal Medicine Baroda Medical CollegeVadodara 390001GujaratIndia Department of Internal Medicine GITAM Institute of Medical Sciences and Research(GIMSR)Visakhapatnam 530045Andhra PradeshIndia Department of Internal Medicine Zaporozhye State Medical UniversityZaporozhye 69000Ukraine Department of Anesthesiology Dr NTR University of Health SciencesVijayawada 520008Andhra PradeshIndia Department of Hematology Center of Hematology and Bone Marrow TransplantationFundeni Clinical InstituteBucharest 022328Romania

BACKGROUND Rotavirus is a highly contagious virus responsible for a significant burden of acute gastroenteritis,particularly among infants and young children worldwide,however,vaccination against this viral agent is *** studies have hypothesized that rotavirus vaccination has been linked to lower rates of antibiotic *** To assess the relationship between rotavirus vaccination and antibiotic *** The present systematic review was tailored based on the Preferred Reporting Items for Systematic reviews and Meta-Analyses *** electronic databases(PubMed/MEDLINE,Scopus and Web of Science)were searched independently by two investigators in order to retrieve relevant publications published until April 2023 that investigated the aforementioned research *** The comprehensive database search identified a total of 91 *** the duplicates were removed(n=75),we screened the titles and abstracts of 16 potentially eligible *** the irrelevant records were excluded(n=5),we screened the full texts of 11 ***,5 studies were entered into the qualitative and quantitative *** In conclusion,all the studies support the idea that vaccinations can reduce the need for antibiotic prescriptions which could potentially contribute to mitigating antibiotic ***,to fully comprehend the mechanisms of antibiotic resistance,enhance treatment guidelines,and consider diverse demographic situations,further research is necessary to use evidence-based strategies to fight antibiotic misuse and resistance.

关键词： Rotavirus Vaccination Gastroenteritis Antibiotics Antibiotic resistance Diarrhea Children

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Preparation and Identification of HLA-A*1101 Tetramer Loading with Human Cytomegalovirus pp65 Antigen Peptide

引用

Cellular & Molecular immunology 2007年第2期4卷 141-146页

作者： Fengyao Li Lihui Xu Qingbing Zha Xiaoyun Chi Qiantao Jia Kianhui He institute of Tissue transplantation and immunology College of Life Science and Technology Jinan University Guangzhou 510632 China institute of Bioengineering College of Life Science and Technology Jinan University Guangzhou 510632 China Clinical Experimental center of the first affiliated hospital Jinan University Guangzhou 510632 China

MHC/peptide tetramer technology has been widely used to study antigen-specific T cells, especially for identifying virus-specific CD8^＋ T cells in humans. The tetramer molecule is composed of HLA heavy chain, β2-microglobulin （β2m）, an antigenic peptide, and fluorescent-labeled streptavidin. To further investigate the HLA-A＊1101-restricted CD8^＋ T cell responses against human cytomegalovirus （HCMV）, we established an approach to prepare HLA-A＊1101 tetramer complexed with a peptide from HCMV. The cDNA encoding HLA-A＊1101 heavy chain was cloned and the prokaryotic expression vector for the ectodomain of HLA-A＊1101 fused with a BirA substrate peptide （HLA-A＊1101-BSP） at its carboxyl terminus was constructed. The fusion protein was highly expressed as inclusion bodies under optimized conditions in Escherichla coli. Moreover, HLA-A＊1101-BSP protein was refolded in the presence of β2m and an HCMV peptide pp6516.24 （GPISGHVLK, GPI）. Soluble HLA-A＊1101-GPI monomer was biotinylated and purified to a purity of 95%, which was subsequently combined with streptavidin to form tetramers at a yield of 〉 80%. The HLA-A＊1101-GPI tetramers could bind to virus-specific CD8^＋ T cells, suggesting soluble HLA-A＊1101-GPI tetramers were biologically functional. This study provides the basis for further evaluation of HLA-A＊1101-restricted CD8^＋ T cell responses against HCMV infection.

关键词： HLA-A* 1101 tetramer cytomegalovirus CD8^+T cell epitope

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Clinical features and survival in aggressive large B cell lymphoma manifesting as hemophagocytic lymphohistiocytosis at diagnosis

Clinical features and survival in aggressive large B cell ly...

引用

浙江省免疫学会第十三次学术大会

作者： Lixia Zhu Mengqi Xiong Lulu Wang Li Li Xiujin Ye bone marrow transplantation center the First Affiliated HospitalZhejiang University School of Medicine

Background:Hemophagocytic lymphohistiocytosis(HLH) is a life-threatening hyperinflammatory ***,HLH presents as a harbinger of aggressive large B cell lymphoma(LBCL),with a rapidly progressive clinical course and poor ***:This study aims to investigate clinical characteristics,outcomes and prognosis factors of aggressive LBCL manifesting as HLH at *** and methods:A retrospective cohort analysis was performed of 30 LBCL patients who initially presenting with HLH between Jan 2020 and September 2022 and March 2021 at our *** clinical parameters and outcomes were *** were followed up until Feb 28,2023 or until ***:At diagnosis,median age was 60 years(range 24-85).All cases presented with an advanced-stage disease(Ⅲ-Ⅳ),20 cases with bone marrow involvement,and 8 with whole-blood Epstein-Barr virus DNA(EBV-DNA)*** were initially treated with ruxolitinib combined with dexamethasone(Ru-D) regimen(44.8%) or HLH-94/04 regimens(17.2%) or dexamethasone only(37.9%) for anti-HLH,and the overall response rate(ORR) was84.6%,40% and 36.4%,*** overall survival(OS) was 439 days,and the 8-week mortality rate was26.7%.Older age(≥68 years),thrombocytopenia(2.5 mmol/L),elevated ferritin(≥3600 ng/L) and EBV-DNA positive were associated with shorter *** analysis showed that thrombocytopenia(hazard ratio [HR],6.801;95% confidence interval [CI],1.96-23.61;P=0.003) and elevated ferritin(HR,3.844;95% CI,1.326-11.146;P=0.013) were independent risk factors for death in this ***:Patients with LBCL accompanied with HLH at diagnosis showed high early mortality and short survival *** data reinforce the necessity to explore the molecular mechanism of HLH occurred at the initial diagnosis of LBCL and develop more effective treatment strategies to improve outcomes.

关键词： hemophagocytic lymphohistiocytosis Epstein-Barr virus B-cell lymphoma

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Long-term results of liver transplantation for over 60 years old patients with hepatitis B virus-related end-stage liver disease

引用

Hepatobiliary & Pancreatic Diseases International 2014年第5期13卷 501-507页

作者： Shu-Hong Yi Hui-Min Yi Bin-Sheng Fu Chi Xu Min-Ru Li Qi Zhang Yang Yang Gui-Hua Chen Liver Transplant center Third Affiliated Hospitaland Transplantation Research Institute Sun Yat-Sen University

BACKGROUND： Hepatitis B virus（HBV）-related end-stage liver disease is the leading indication for liver transplantation in China, but long-term results of liver transplantation in patients aged over 60 years are not clear. The present study was to reveal the natural history of liver recipients with hepatitis B older than60 ***： The recipients who had received liver transplantation between December 2003 and December 2005 were divided into two groups： those equal or older than 60 years（older group,n60） and those younger than 60 years（younger group, n305）.Risk factors for poor long-term outcome in patients aged over 60 years were also ***： Except for age and preexisting chronic disease（P0.05）,no significant differences were observed in perioperative characteristics between the two groups. There was also no significant difference in HBV and hepatocellular carcinoma recurrence（P0.05）. The actuarial 1-, 3-, 5- and 8-year survival rates were 81.6%, 71.6%, 66.7% and 63.3% respectively for the older group vs 84.9%, 77.7%, 70.8% and 65.6% for the younger group（P0.05）. Multivariate analyses showed that pre-liver transplant renal insufficiency was a risk factor for poor outcome in the older group（odds ratio=3.615, P0.014）.CONCLUSIONS： Liver transplantation is safe and feasible for patients with HBV-related end-stage liver disease aged over 60years. Older patients with renal insufficiency should undergo transplantation earlier than younger patients.

关键词： ge hepatitis B virus liver transplantation renal insufficiency long-term

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

mTORC1 Regulates the Immunosuppressive Properties of Mesenchymal Stem Cells through Prostaglandin E2

mTORC1 Regulates the Immunosuppressive Properties of Mesench...

引用

第十一届全国免疫学学术大会

作者： Wang Binsheng Lin Yu Hu Yongxian Shan Wei Liu Senquan Xu Yulin Zhang Hao Cai Shuyang Yu Xiaohong Cai Zhen Huang He bone marrow transplantation center The First Affiliated Hospital College of Medicine Zhejiang University

Objectives:The aim of this study is to clarify the effects and mechanisms of m TORC1 on regulation the immunosuppressive ability of mesenchymal stem cells(MSCs).Methods:After pretreatment with rapamycin for different ... 详细信息

关键词： m TORC1 mesenchymal stem cells immunosuppressive function PGE2

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：