检索结果-南通市图书馆

Deciphering fungal metabolon coupling tandem inverse-electrondemand Diels-Alder reaction and semipinacol rearrangement for the biosynthesis of spiro polycyclic alkaloids

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2025年第01期 288-296页

作者： Shuai Liu Wen-Qiang Xu Ying-Tong Di Man-Cheng Tang Ding-Kang Chen Ming-Ming Cao Yao-Wen Chang Hong-Yu Tang Chun-Mao Yuan Jun-Bo Yang Zhi-Li Zuo Han Guo Zi-Fei Xu Ying Zeng Yun-Dong Wu Xiao-Jiang Hao Key Laboratory of Phytochemistry and Natural Medicines (Chinese Academy of Sciences) Kunming Institute of Botany Chinese Academy of Sciences University of Chinese Academy of Sciences Lab of Computational Chemistry and Drug Design State Key Laboratory of Chemical Oncogenomics Peking University Shenzhen Graduate School Shenzhen Bay Laboratory State Key Laboratory of Microbial Metabolism School of Life Sciences & Biotechnology Shanghai Jiao Tong University State Key Laboratory of Functions and Applications of Medicinal plants Guizhou Medical University Molecular biology experimental Center Germplasm Bank of Wild Species in Southwest China Kunming Institute of Botany Chinese Academy of Sciences Yunnan Key Laboratory for Wild plant Resources Kunming Institute of Botany Chinese Academy of Sciences Research Unit of Chemical biology of Natural Anti-Virus Products Chinese Academy of Medical Sciences

In the intricate process of natural product biosynthesis, a metabolon can enhance metabolic flux by associating sequential enzymes. A fungal metabolon, comprising of flavin-dependent monooxygenase Spe F and P450 monooxygenase Spe G, is identified in the biosynthesis of spiro polycyclic alkaloids(+)-notoamide B and its diastereomer(+)-versicolamide B. Using notoamide E as a substance, Spe F/Spe G metabolon can control the stereoselectivity of its 2,3-epoxidation, followed by hydrogen atom ion at C-17 to generate reactive epoxide tau-m A with dienyl iminium unit. Subsequently,(+)-notoamide B and(+)-versicolamide B are produced via tandem nonenzymatic inverse-electron-demand Diels-Alder reaction and semipinacol rearrangement. This provides the first example of metabolon in the biosynthesis of spiro-prenylated indole alkaloids.

关键词：

同方期刊数据库

Characterization of an ethylene receptor homolog gene from rice

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2003年第4期46卷 370-378页

作者：曹宛虹董亿张劲松陈受宜 plant Biotechnology Lab Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Ethylene plays important roles in plant growth, development and stress responses. Its receptor genes have been studied in dicots such as Arabidopsis, tobacco and tomato. However, no research has been reported for the ethylene receptors from monocots currently. In the present study, we cloned an ethylene receptor gene OSPK2 from rice and found that its encoded protein was divergent from the ethylene receptors from dicots. OSPK2 had a long extension in its N- terminal, followed by three transmembrane segments, a GAF domain, a putative kinase domain and a putative receiver domain. Although most of the domains were conserved, the expected phosphorylation site His and the phosphate receiver Asp have been replaced by Gln and Asn, respectively. This fact indicates that OSPK2 may not function as a histidine kinase in a phosphorelay manner, but rather play roles by other mechanism, probably through Ser/Thr kinase activity. The expression of the OSPK2 gene was investigated by RT-PCR method under different conditions. We found that this gene was apparently induced by wounding and PEG treatment, but not significantly affected by salt and ABA treatments. The differential expression of the OSPK2 gene may reflect its roles in mediating different abiotic stress responses, consistent with our previous studies on tobacco ethylene receptors.

关键词： ethylene receptor, rice, stress, expression.

Homeodomain leucine-zipper proteins and their role in synchronizing growth and development with the environment

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative plant biology 2014年第6期56卷 518-526页

作者： Ronny Brt Marc Cabedo Yakun Xie Stephan Wenkel Center for plant Molecular biology University of Tübingen Germany Leibniz institute of plant genetics and Crop plant Research GaterslebenGermany

The Arabidopsis （Arabidopsis thaliana L.） genome encodes for four distinct classes of homeodomain leucinezipper （HD-ZIP） transcription factors （HD-ZIPI to HD-ZIPIV）, which are all organized in multi-gene families. HD-ZIP transcription factors act as sequence-specific DNA-binding proteins that are able to control the expression level of target genes. While HD-ZIPI and HD-ZIPII proteins are mainly associated with environmental responses, HD-ZIPIII and HD- ZIPIV are primarily known to act as patterning factors. Recent studies have challenged this view. It appears that several of the different HD-ZlP families interact genetically to align both morphogenesis and environmental responses, most likely by modulating phytohormone-signaling networks.

关键词： Transcription factors homeodomain leucine zipper auxin light signaling water stress abscisic acid leaf development REVOLUTA KANADI microRNA

plant pattern-recognition receptors controlling innate immunity

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2016年第9期59卷 878-888页

作者： Lei Li Yufei Yu Zhaoyang Zhou Jian-Min Zhou State Key Laboratory of plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences

plants are exposed to numerous potential pathogenic microbes. To counter the threat, plants have evolved diverse patternrecognition receptors(PRRs), which are receptor kinases(RKs) and receptor proteins(RPs) specialized to detect conserved pathogen/microbe-associated molecular patterns(PAMPs/MAMPs). Although only a handful of RKs and RPs are known PRRs,they belong to the receptor-like kinase(RLK) and receptor-like protein(RLP) superfamilies that undergo lineage-specific expansion, suggesting that many of these RLKs and RLPs are potential PRRs. Analyses of existing PRRs have uncovered ligand-induced RLK-RK or RLK-RP oligomerization as a common mechanism for immune activation. PRRs can recruit additional components to form dynamic receptor complexes, which mediate specific cellular responses. Detailed analyses of these components are shedding light on molecular mechanisms underlying the regulation of PRR activity and downstream signaling.

关键词： receptor kinase receptor protein receptor-like cytoplasmic kinase plant innate immunity

The secret of fertilization in flowering plants unveiled

在线全文

学校读者我要写书评

暂无评论

Science Bulletin 2018年第7期63卷 408-410页

作者： Xiangxiu Liang Jian-Min Zhou State Key Laboratory of plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences

As recapitulated in the famous Chinese fairy tale Journey to the West,the Buddhist monk Xuanzang of the Tang Dynasty(Tangseng)took a heroic journey in a quest for India Buddhist sacred *** long and dangerous journey was made possible only with the help of the powerful Monkey King,who had been pinned down under a mountain by the Buddha as a punishment for his prior *** Buddha had to secure the

关键词：授精植物旅行猴子佛家佛像封口

Non-26S Proteasome Proteolytic Role of Ubiquitin in plant Endocytosis and Endosomal Trafficking

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative plant biology 2013年第1期55卷 54-63页

作者： Miaomiao Tian Qi Xie State Key Laboratory of plant Genomics National Center for Plant Gene Research Institute of Genetics and Developmental Biologythe Chinese Academy of Sciences

The 76 amino acid protein ubiquitin （Ub） is highly conserved in all eukaryotic species. It plays important roles in many cellular processes by covalently attaching to the target proteins. The best known function of Ub is marking substrate proteins for degra- dation by the 26S proteasome. In fact, other consequences of ubiquitination have been discovered in yeast and mammals, such as membrane trafficking, DNA repair, chromatin modification, and protein kinase activation. The common mechanism underlying these processes is that Ub serves as a signal to sort proteins to the vacuoles or lysosomes for degradation as opposed to 26S proteasome-dependent degradation. To date, several reports have indicated that a similar function of Ub also exists in plants. This review focuses on a summary and analysis of the recent research progress on Ub acting as a signal to mediate endocytosis and endosomal trafficking in plants.

关键词： Endocytosis endosomal trafficking ubiquitin.

Receptor-Like Kinases in plant Innate Immunity

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative plant biology 2013年第12期55卷 1271-1286页

作者： Ying Wu Jian-Min Zhou State Key Laboratory of plant Genomics and National Center for plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of Sciences

plants employ a highly effective surveillance system to detect potential pathogens, which is critical for the success of land plants in an environment surrounded by numerous microbes. Recent efforts have led to the identification of a number of immune receptors and components of immune receptor complexes. It is now clear that receptor-like kinases （RLKs） and receptor-like proteins （RLPs） are key pattern-recognition receptors （PRRs） for microbe- and plant-derived molecular patterns that are associated with pathogen invasion. RLKs and RLPs involved in immune signaling belong to large gene families in plants and have undergone lineage specific expansion. Molecular evolution and population studies on phytopathogenic molecular signatures and their receptors have provided crucial insight into the co-evolution between plants and pathogens.

关键词： Receptor-like kinase (RLK) receptor-like protein (RLP) cytoplasmic RLK (RLCK) pathogen-associated molecular pattern (PAMP) damage-associated molecular pattern (DAMP) plant innate immunity.

From Basic Research to Molecular Breeding——Chinese Scientists Play A Central Role in Boosting World Rice Production

在线全文

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2018年第6期16卷 389-392页

作者： Ding Tang Zhukuan Cheng State Key Laboratory of plant Genomics and Center for plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of Sciences

On November 18, 2018, the Future Science Prize Awarding Ceremony was held in Beijing. In the area of life science, Professors Jiayang Li, Longping Yuan, and Qifa Zhang shared the prize for their pioneering contributions in producing high-yield, superior-quality rice through systematic study of molecular mechanisms associated with speci?c rice features and application of novel approaches in rice breeding. The Future Science Prize is also touted as ‘‘China’s Nobel Prize", fully af?rming their achievements in rice basic research and breeding.

关键词： Zhang In From Basic Research to Molecular Breeding GHD

Wheat PROTON GRADIENT REGULATION 5 is Involved in Tolerance to Photoinhibition

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2014年第6期13卷 1206-1215页

作者： WANG Yuan-ge HE Xue MA Wen-ying ZHAO Xue-qiang LI Bin TONG Yi-ping The State Key Laboratory for plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences

Wheat （Triticum aestivum L.） often experiences photoinhibition due to strong light during the grain filling stage. As such, increasing the tolerance of wheat to photoinhibition is very desirable in breeding efforts focused on increasing grain yields. Previous reports have suggested that PROTON GRADIENT REGULATION 5 （PGR5） plays a central role in the generation of a proton gradient across the thylakoid membrane （ApH） and in acclimation to high light intensity conditions. Three PGR5 homoeologues were isolated from wheat, and mapped onto chromosomes 7A, 7B and 7D, respectively. The TaPGR5s shared highly similar genomic sequences and gene structures. The transcripts of TaPGR5s were found to be abundantly expressed in the flag leaves, and were transiently up-regulated by treatment with high light. High light treatment inhibited the net photosynthetic rate （Pn） and the maximal quantum yield ofphotosystem II （Fv/Fm）. Further, these inhibitions were more evident in the leaves with reduced expression of TaPGR5s achieved using virus-induced gene silencing methods. Moreover, reducing TaPGR5 expression impaired the induction of non-photochemical quenching （NPQ）, which caused more severe cell membrane damage and lipid peroxidation in high light. Additionally, we observed that TaPGR5s transcripts were more abundantly expressed in the wheat genotypes with higher ms-delayed light emission （ms-DLE）, a value reflecting transthylakoid ApH. These results suggested that TaPGR5s play important roles in the tolerance of wheat to photoinhibition.

关键词： wheat TaPGR5 high light stress photoinhibition photosynthesis