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Exploring the taste presentation and receptor perception mechanism of salty peptides from Stropharia rugosoannulata based on molecular dynamics and thermodynamics simulation

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Food Science and Human Wellness 2024年第4期13卷 2277-2288页

作者： Wen Li Shuai Sun Wanchao Chen Haile Ma Tingzhao Li Zhong Zhang Di Wu Mengqiu Yan Yan Yang institute of edible fungi Shanghai Academy of Agricultural SciencesNational Engineering Research Center of Edible FungiKey Laboratory of Edible Fungi Resources and Utilization(South)Ministry of Agriculture and Rural AffairsShanghai 201403China School of Food&Biological Engineering Institute of Food Physical ProcessingJiangsu UniversityZhenjiang 212013China Amway(Shanghai)Innovation&Science Co. Ltd.Shanghai 201203China Amway(China)Botanical R&D Center Wuxi 214115China

The taste presentation and receptor perception mechanism of the salty peptide of Stropharia rugosoannulata were predicted and verified using peptide omics and molecular interaction techniques.The combination of aspartic acid(D)and glutamic acid(E),or peptide fragments composed of arginine(R),constitute the characteristic taste structural basis of salty peptides of S.rugosoannulata.The taste intensity of the salty peptide positively correlates with its concentration within a specific concentration range(0.25–1.0 mg/mL).The receptor more easily recognizes the first amino acid residue at the N-terminal of salty peptides and the aspartic acid residue in the peptides.GLU513,ASP707,and VAL508 are the critical amino acid residues for the receptor to recognize salty peptides.TRPV1 is specifically the receptor for recognizing salty peptides.Hydrogen bonds and electrostatic interactions are the main driving forces for the interactions between salty peptides and TRPV1 receptors.KSWDDFFTR has the most potent binding capacity with the receptor and has tremendous potential for application in sodium salt substitution.This study confirmed the taste receptor that specifically recognizes salty peptides,analyzed the receptor-peptide binding interaction,and provided a new idea for understanding the taste receptor perception of salty peptides.

关键词： Salty peptide Molecular docking Biolayer interferometry Isothermal titration calorimetry

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Polyphenol-rich extract from Sanghuangporous vaninii against acetaminophen-induced liver injury by blocking ferroptosis via NRF2/GPX4 pathway

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Food Science and Human Wellness 2024年

作者： Peng Liu Yuyang Wang Wanchao Chen Di Wu Zhong Zhang Zhengpeng Li Anqiang Zhang Wen Li Yan Yang institute of edible fungi Shanghai Academy of Agricultural Sciences National Engineering Research Center of edible fungi Key Laboratory of edible fungi Resources and Utilization (South) Ministry of Agriculture College of Food Science & Technology Shanghai Ocean University College of Food Science and Engineering Zhejiang University of Technology

Acetaminophen (APAP) used as an antipyretic and analgesic agent can cause acute liver injury (AILI) under overdose. Sanghuangporous vaninii (S. vaninii) is a edible fungus with abundant metabolites exhibits excellent hepatoprotective activities， but the effect for AILI is not yet fully understood. In this study， the polyphenol-rich extract from S. vaninii (PSV) was prepared， with a total phenolic content of 75.72% and 34 compounds. The data of hepatoprotection indicated that PSV obviously alleviated the hepatocellular injury induced by APAP in vivo and in vitro. The protective mechanism of PSV against APAP-induced AILI might be attributed to the activating NRF2/GPX4 pathway. Based on network pharmacology analysis， the active components of PSV such as caffeic acid， osmundacetone and hispolone played a key role in hepatoprotection of PSV. Consequently， this study highlights the protection of PSV on AILI， which provides new insight into bioactivities of S. vaninii.

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The ERF transcription factor LTF1 activates DIR1 to control stereoselective synthesis of antiviral lignans and stress defense in Isatis indigotica roots

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Acta Pharmaceutica Sinica B 2024年第1期14卷 405-420页

作者： Ruibing Chen Jian Yu Luyao Yu Liang Xiao Ying Xiao Junfeng Chen Shouhong Gao Xianghui Chen Qing Li Henan Zhang Wansheng Chen Lei Zhang Department of Pharmaceutical Botany School of PharmacyNaval Medical UniversityShanghai 200433China Research and Development Center of Chinese Medicine Resources and Biotechnology Shanghai University of Traditional Chinese MedicineShanghai 201203China Department of Pharmacy Shanghai Changzheng HospitalNaval Medical UniversityShanghai 200003China School of Medicine Shanghai UniversityShanghai 200433China institute of edible fungi Shanghai Academy of Agricultural SciencesNational Engineering Research Center of Edible FungiKey Laboratory of Edible Fungi Resources and Utilization(South)Ministry of AgricultureShanghai 201403China College of Life Sciences and Medicine Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang ProvinceZhejiang Sci-Tech UniversityHangzhou 310018China State Key Laboratory of Dao-di Herbs Beijing 100700China

Lignans are a powerful weapon for plants to resist stresses and have diverse bioactive functions to protect human health.Elucidating the mechanisms of stereoselective biosynthesis and response to stresses of lignans is important for the guidance of plant improvement.Here,we identified the complete pathway to stereoselectively synthesize antiviral(-)-lariciresinol glucosides in Isatis indigotica roots,which consists of three-step sequential stereoselective enzymes DIR1/2,PLR,and UGT71B2.DIR1 was further identified as the key gene in respoJanuary 2024nse to stresses and was able to trigger stress defenses by mediating the elevation in lignan content.Mechanistically,the phytohormone-responsive ERF transcription factor LTF1 colocalized with DIR1 in the cell periphery of the vascular regions in mature roots and helped resist biotic and abiotic stresses by directly regulating the expression of DIR1.These systematic results suggest that DIR1 as the first common step of the lignan pathway cooperates with PLR and UGT71B2 to stereoselectively synthesize(-)-lariciresinol derived antiviral lignans in I.indigotica roots and is also a part of the LTF1-mediated regulatory network to resist stresses.In conclusion,the LTF1-DIR1 module is an ideal engineering target to improve plant Defenses while increasing the content of valuable lignans in plants.

关键词： Lignans Stereoselective synthesis Stress resistance Dirigent protein ERF

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Yolk free egg substitute improves the serum phospholipid profile of mice with metabolic syndrome based on lipidomic analysis

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Food Science and Human Wellness 2024年第1期13卷 482-493页

作者： Zhihui Yu Lingyu Fan Fei Tai Lixin Zhang Xiaoyu Zhang Yisheng Chen College of Food Science and Engineering Shanxi Agricultural UniversityTaigu 030801China Insitute of Food Nutrition and Safety Shanxi Agricultural UniversityTaiyuan 030031China Shanxi Key Laboratory of edible fungi for Loess Plateau Taigu 030801China

In this study,the impacts of egg consumption on mice model of metabolic syndrome(Met S)were comparatively investigated.Mice were divided into five groups(n=8):normal diet group(ND),high-fat diet group(HFD),HFD with whole egg group(WE),HFD with free-yolk egg substitute group(YFES),and HFD with lovastatin group(Lov).Main biochemical indexes and a non-targeted lipidomic analysis were employed to insight the lipid profile changes in serum.It was revealed that WE could significantly improve serum biochemical indexes by reducing body weight,low-density lipoprotein cholesterol(LDL-C)and total cholesterol(TC),while increasing high-density lipoprotein cholesterol.YFES exhibited remarkably better performance in increasing phosphatidylglycerol and phosphatidic acids,while decreasing phosphatidylinositol than WE.A total of 50 differential lipids biomarkers tightly related to glycerophospholipids metabolism were screened out.Carnitine C18:2 and C12:1,SM(d18:0/12:0),and SM(d18:1/14:1)were significantly upregulated in YFES compared to WE.YFES reduced expression of SREBP-1c and Cpt1a,while did not affect the expression of PPAR-α.Sphingomyelin biomarkers were positively related to the TC(|r|>0.6),while PPAR-αwas negatively correlated with triglyceride and LDL-C levels.To sum up,YFES attenuated HFD-induced Met S by improving the serum phospholipids,which account for its modulation of glycerophospholipid metabolism.

关键词： Metabolic syndrome Whole egg Yolk free egg substitute Serum Lipidomics

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Transcriptome and proteome analysis reveals the characteristics of carbon metabolism in the mycelial development of Gomphus purpuraceus

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Food Science and Human Wellness 2024年

作者： Yangyang Geng Shixin Zhang Yana Liu Likang Qin School of Liquor and Food Engineering Guizhou University Guizhou institute of Walnut Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education) College of Life Sciences/institute of Agro-bioengineering Guizhou University Guizhou Academy of Forestry Guizhou Industrial Technology Research institute of Rare edible and Medicinal fungi co. Ltd

Gomphus purpuraceus is an ancient wild edible mushroom. However， the niche and lignocellulose degradation system of G. purpuraceus have remained cryptic. In the current study， transcriptome and proteome sequencing of G. purpuraceus in four mediums was performed. A total of 3489 differentially expressed genes (DEGs) and 891 differentially expressed proteins (DEPs) were screened out. For the lignocellulose degradation system， a total of 61 expressed genes were annotated， including 19 genes encoding cellulase， 8 genes encoding hemicellulase， 5 genes encoding pectinase， 22 genes encoding lignin oxidase， and 7 lignin degrading auxiliary enzyme. From two-omics， morphology， and enzyme activities， we speculated that G. purpuraceus might have a limited capacity to degrade polysaccharides (starch and lignocellulose). In addition， trace amount of fumonisins (FB1 and FB2) were found in the G. purpuraceus. These results provided a new perspective for understanding the key pathways and hub genes involved in carbon metabolism of G. purpuraceus development.

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Cloning of a Putative Pheromone Receptor Gene Fragment from Lentinula edodes

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食用菌学报 2008年第2期15卷 4-6页

作者： BAO Dapeng ZHANG Meiyan LI Su CHEN Mingjie Shanghai Research Center for edible fungi Biotechnology and Engineering Key Laboratory of Agricultural Genetics and Breeding of Shanghai Institute of Edible Fungi Shanghai Academy of Agricultural Sciences Shanghai 201106 China

A 508 bp fragment of a putative pheromone receptor gene from Lentinula edodes was amplified by PCR using a pair of degenerate primers designed according to conserved sequences of pheromone receptor genes reported in other Basidiomycetes.The fragment encodes 117 amino acids and contains three introns,56 bp,51 bp and 50 bp in size.Alignment analysis revealed that the deduced amino acid sequence of the L.edodes fragment exhibited 65% identity and 82% similarity with the corresponding segments of pheromone receptors reported in Coprinus cinereus and Schizophyllum commune.

关键词：克隆技术信息素受体基因

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Flavone Content and Bioactivity of Aqueous-Ethanolic Extracts of Phellinus Fruit Bodies

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食用菌学报 2008年第2期15卷 27-30页

作者： HAO Ruixia ZHOU Shuai YANG Yan XI Junzhe LI Weiyao TANG Qingjiu WANG Chenguang Shanghai Biotechnology and Engineering Research Center for the edible fungi Key Laboratory of Agricultural Genetics and Breeding of Shanghai Research Center of Natural Medicinal Resources Institute of Edible Fungi Shanghai Academy of Agricultural Sciences Shanghai 201106China College of Food Science and Technology Shanghai Fisheries University Shanghai 200090 China

The flavone content of aqueous ethanolic extracts of Phellinus fruit bodies obtained from seven different locations was determined.Anti-tumor and antioxidant activities associated with the extracts were investigated in vitro using the Alamar Blue assay and a chemiluminecence-based method,respectively.Extracts of the seven Phellinus samples generated very similar HPLC profiles in qualitative terms although the areas of several peaks varied according to the sample type indicating quantitative differences in the case of some components.All the extracts,especially those of artificially cultivated Phellinus baumii fruit bodies,inhibited L1210 tumor cell proliferation and exhibited superoxide radical scavenging activity although there was no obvious correlation with flavone content.

关键词：生物活性果实黄酮含量抗癌物质

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Isolation,Purification and Immunomodifying Activity of Polysaccharides from Phellinus igniarius

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食用菌学报 2008年第2期15卷 37-41页

作者： SHEN Xuexiang YANG Yan ZHANG Jinsong TANG Qingjiu JIA Wei Shanghai Research Center for edible fungi Biotechnology and Engineering Key Laboratory ofAgricultural Genetics and Breeding of Shanghai Research Center of Natural Medicinal Resources Institute of Edible Fungi Shanghai Academy of Agricultural Sciences Shanghai 201106 China College of Food Science and Technology Shanghai Fisheries University Shanghai 200090 China

Phellinus igniarius polysaccharides were obtained by hot water extraction of mushroom fruit bodies followed by ethanol precipitation,and further purified by anion exchange and gel filtration chromatography.Immuno-enhancing activity was evaluated by determining the effect of different polysaccharide fractions on mouse spleen lymphocyte proliferation in vitro.Three crude polysaccharide preparations,designated PIP30,PIP60 and PIP80 according to the percentage concentration of ethanol required for precipitation from hot aqueous extracts,promoted lymphocyte proliferation to varying degrees.Purification of polysaccharide present in PIP30 and PIP60 was carried out using anion exchange chromatography.Proliferation rates in samples treated with 200 μg/mL of fractions P30U,P30W and P30S1(obtained following anion exchange chromatography of PIP30) were approximately 5-,4-,and 3.5-fold higher,respectively compared with negative controls.Similarly,marked increases(3.4-and 2.8-fold) in cell proliferation rates compared with negative controls were observed with 200 μg/mL concentrations of P60W and P60S2(obtained following anion exchange chromatography of PIP60),respectively.Two purified polysaccharide preparations,P60W1-1 and P60S1-1,were obtained following gel filtation chromatography of fractions P60W1 and P0S1,respectively.Fraction P60W1-1(10-100 g/mL) enhanced splenocyte proliferation 0.5-1.4-fold compared with negative controls,whereas no effects were observed with P60S1-1.

关键词：纯化工艺多糖生物活性色谱法

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Luteolin， a common flavone in vegetables， acts as a hormetin possessing neurotrophic function: signaling mediated by inducing mitochondrial stress

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Food Science and Human Wellness 2024年

作者： Alex Xiong Gao Tracy Chenxi Xia Maggie Suisui Guo Gary Kawing Yuen Kevin Qiyun Wu Tina Tingxia Dong Karl Wah-Keung Tsim Shenzhen Key Laboratory of edible and Medicinal Bioresources HKUST Shenzhen Research institute Division of Life Science and Center for Chinese Medicine The Hong Kong University of Science and Technology

This study aims to uncover the hormetic process of luteolin， a common dietary flavone， in neuronal cells through its role in inducing mitochondrial stress. In rat pheochromocytoma PC12 cells， luteolin at low concentrations caused a mild and reversible loss of mitochondrial membrane potential (MMP)， while high concentrations of luteolin triggered intense and sustained depolarization of MMP. The MMP disturbance was shown to have a close association with the trophic and/or toxic effects triggered by luteolin; because the common mitochondrial uncouplers shared similar bi-phase dose-response on cell viability， as that of luteolin. Along with the induced MMP pertubation， luteolin triggered the development of autophagy and mitophagy， as determined by mCherry-GFP-LC3B tandem protein， and by the co-localization of mitochondrial/lysosomal staining. Subsequent application of autophagy inhibitors in the cultures blocked the luteolin-induced neurotrophic activities and sensitized the cells to be less resistant to luteolin-mediated cytotoxicity. Other flavonoids also displayed similar properties in the cultures， indicating that these compounds act as hormetic pharmacological inducers that stimulate cells to become more resilient and adapt to threats. This study provides a unifying mechanistic explanation for the neuro-beneficial effects of luteolin and other flavonoids.

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3-Epi-betulinic acid 3-O-β-D-glucopyranoside(eBAG)induces autophagy by activation of AMP-activated protein kinase in hepatocellular carcinoma

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Food Science and Human Wellness 2024年第3期13卷 1453-1464页

作者： Mengjie Liu Xuqiang Liu Kaiyue He Yongping Jian Yujia Li Jinrong Guo Jingyu Yang Zhixiang Xu Wenyi Kang School of Life Sciences Henan UniversityKaifeng 475004China National R&D Center for edible Fungus Processing Technology Henan UniversityKaifeng 475004China Joint International Research Laboratory of Food&Medicine Resource Function Kaifeng 475004China Shenzhen Research institute of Henan University Shenzhen 518000China

3-Epi-betulinic acid 3-O-β-D-glucopyranoside(eBAG)is a pentacyclic triterpene mainly distributed in food and medicinal plants,which exhibits various pharmacological properties.However,whether these functions are attributed to eBAG or additional components in these plants remain unknown.Herein,we report that eBAG exerted an inhibitory activity against hepatocellular carcinoma and esophageal cancer cells.EBAG induced non-apoptotic cell death in hepatocellular carcinoma cells.The eBAG-induced cell death was inhibited by knock-down of autophagy related gene(ATG)5 and ATG7,by administration of 3-methyladenine,a selective autophagy inhibitor that suppresses phosphoinositide 3-kinase(PI3K),and by chloroquine,a classic autophagy flux inhibitor.We demonstrated that eBAG induced an autophagy-mediated cell death.Application of eBAG mimicked cellular bioenergetics depletion leading to the reduction of intracellular ATP,activation of AMP-activated protein kinase(AMPK),and inhibition of mTOR.Co-treatment with compound C,an AMPK inhibitor,abrogated cell death induced by eBAG.We further validated the anti-tumor effect of eBAG in the murine xenograft model of hepatocellular carcinoma and found that eBAG treatment promoted the induction of autophagy and reduction of tumor growth in mice.As a functional food ingredient,eBAG is a potential therapeutic agent for the treatment of hepatocellular carcinoma and esophageal cancer.

关键词： eBAG Autophagy ATP AMPK Hepatocellular carcinoma

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