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LC2 and OsVIL2 Promote Rice Flowering by Photoperoid-lnduced Epigenetic Silencing of OsLF

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Molecular Plant 2013年第2期6卷 514-527页

作者： Jun Wang Jiang Hu Qian Qian Hong-Wei Xue National Key Laboratory of Plant Molecular genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chineseacademy of Sciences Shanghai 200032 People's Republic of China State key Laboratory of Rice Biology China National Rice Research institute Hangzhou Zhejiang 310006 People's Republic of China

Proper flowering time is essential for plant reproduction. Winter annual Arabidopsis thaliana needs ver-nalization before flowering, during which AtVILs （VIN3 and VRNS, components of PRC2 complex） mediate the H3K27 tri- methylation at the FLC locus （a floral repressor） to repress the FLC expression and hence to induce flowering. However, how VILs （VIL, VERNALIZATION INSENSITIVE 3-LIKE） function in rice is unknown. Here we demonstrated that rice LC2 （OsVIL3） and OsVIL2 （two OsVILs, possible components of PRC2 complex） promote rice flowering. Our results showed that expressions of LC2 and OsVIL2 are induced by sd （short-day） conditions and both Ic2 mutant and OsVIL2-RNAi lines display delayed heading date, consistent with the reduced expression levels of Hdl and Hd3a. Interestingly, LC2 binds to the promoter region of a floral repressor OsLF and represses the OsLF expression via H3K27 tri-methylation modification. In addition, OsLF directly regulates the Hdl expression through binding to Hdl promoter. These results first demonstrated that the putative PRC2 in rice is involved in photoperiod flowering regulation, which is different from that of Arabidopsis, and revealed that LC2 binds the promoter region of target gene, presenting a possible mechanism of the recruitment pro-cess of PRC2 complex to its target genes. The studies provide informative clues on the epigenetic control of rice flowering.

关键词： epigenetic control rice flowering LC2 PRC2.

Synthetic Peptides Affect the Expression of Gdnf and Gdnf Receptors in Rats with 6-OHDA-Induced PD-Like Parkinsonism

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World Journal of Neuroscience 2016年第4期6卷 243-259页

作者： Elena V. Filatova Maria I. Shadrina Timur A. Kolomin Ludmila A. Andreeva Nikolay F. Myasoedov Petr A. Slominsky institute of Molecular genetics Russian Academy of Sciences Moscow Russia

Parkinson’s disease (PD) is the second most common severe neurodegenerative disorder. It is characterized by progressive degeneration of dopaminergic neurons in the substantia nigra pars compacta. Unfortunately, PD remains incurable. Therapy based on regulatory peptides, particularly neuroprotective peptides, which can sustain or activate neuron plasticity to enable their survival and function in difficult environments and after violated homeostasis, is a promising approach to cure PD. Some studies show that the synthetic analogs of natural peptides may be used as an etiological or at least a complementary therapy in PD. Therefore, in the present pilot study, we investigated the effects of the synthetic peptides Semax and dopamine neuron stimulating peptide (DNSP-5), and a new synthetic Semax-DNSP-5 hybrid peptide (sd) on the functioning of brain neurons. An analysis of the levels of dopamine (DA), noradrenaline (NA), 5-hydroxytriptamine (5-HT), an expression analysis of Gdnf and Gdnf receptor genes Gfra1, Gfra2, Gfra3, Gfra4, and Gfral in various regions of the brain of rats with 6-OHDA-induced PD-like parkinsonism, and a study of the motor activity of the rats in an “open field” test showed that DNSP-5 and sd elevated the level of DA in the nonlesioned striatum. DNSP-5 also increased the expression of Gfra1 and Gfra2 in the nonlesioned striatum and lesioned substantia nigra (SN) which suggested that DNSP-5 had compensatory and neuroprotective properties. sd demonstrated similar, albeit less pronounced effects to DNSP-5 on DA metabolism and gene expression. Of the peptides studied, only sd tended to increase the horizontal and vertical activity of rats. In conclusion, these findings suggest that DNSP-5 and sd have potential neuroprotective properties and may stimulate the surviving DA neurons.

关键词： 6-OHDA Parkinson’s Disease Semax DNSP-5 Gene Expression

Psychosocial Factors and the Risk of Type 2 Diabetes Mellitus in Women Population based Epidemiological Study

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Journal of Geriatric Medicine 2020年第2期2卷 1-10页

作者： Valery Gafarov Dmitriy Panov Elena Gromova Igor Gagulin Almira Gafarova institute of Internal and Preventive Medicine-branch of institute of cytology and genetics SB ras NovosibirskRussian Federation Collaborative laboratory of Cardiovascular Diseases Epidemiology NovosibirskRussian Federation

Our aim was to study the effect of depression and social support on the risk of type 2 diabetes in female population aged 25-64 in Russia/*** the screening surveys random representative samples of women aged 25-64 years were examined in 1994 and *** assessment was performed using the MONICA-MOPSY *** support was measured using the Berkman-Sim *** 1994 to 2018 in a cohort of women new-onset cases of diabetes mellitus were *** risk of T2DM in persons with depression was 1.844(p<0.01).After adjusting for sociodemographic variables,the risk decreased by 6%but remained significantly significant(p<0.05).The impact of a low level of social relations showed a significant effect on the risk of diabetes mellitus,including the multivariate model adjusted for the social gradient(HR=1.833,p<0.05).The presence of psychosocial factors decreases the protective effect of education in diabetes *** incidence of T2D was higher in the group of manual labor and in *** and low social support increase the risk of T2DM by 80%.The frequencies of T2DM are determined by the social gradient and are associated with the role conflict“family-work”.

关键词： Depression Social support Diabetes Female population Risk

Study on the Obtaining of Transgenic Wheat with GNA Alien Gene by Biolistic Particle

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Agricultural Sciences in China 2002年第1期1卷 25-29页

作者： XU Qiong-fang, LI Lian-cheng, CHEN Xiao, TIAN Fang, MA You-zhi, YE Xing-guo, ZHANG Zeng-yan, XU Hui-jun and XIN Zhi-yong( Key Laboratory of Agricultural Ministry for Crop genetics and Breeding, institute of Crop Breeding and Cultivation ,Chinese Academy of Agricultural Sciences, Beijing 100081 ) Key Laboratory of Agricultural Ministry for Crop genetics and Breeding Institute of Crop Breeding and Cultivation Chinese Academy of Agricultural Sciences Beijing 100081

The immature embryos of wheat plants, cv. Jing 411, 12 - 14 days after pollination, were cultured on sd2 medium for callus induction. After 10 days culture, 800 wheat calli were bombarded by biolistic particle coated with the DNA of plasmid pBI121-2 harboring both Galanthus nivalis agglutinin gene and bar gene. 67 green plants were finally regenerated from the bombardment calli on selection medium containing 4mg/L Basta. The results of bioassay by both inoculating wheat aphids onto the plants and applying Basta solution of 50 mg/L and 75 mg/L onto the wheat leaves in the field, and the molecular analysis, such as PCR and Southern blotting, indicated that 8 T2 plants contaning the target genes were obtained.

关键词： Galanthus nivalis agglutinin bar gene Particle bombardment Transgenic wheat

High expression of synthetic human interferon-γ cDNA in ***

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Science China Chemistry 1995年第9期38卷 1084-1093页

作者：王子轩杨迪王启松李伯良吕兆海虞建良郑宏大范佩芳唐建伟钱民章郭海鸿姚曼华郑仲承刘新垣 Shanghai institute of Biochemistry Chinese Academy of SciencesShanghai 200031China institute of genetics Fudan UniversityShanghai

Human interferon-γ（IFN-γ）cDNA was synthesized,and it makes the usage of favorablecodons in *** authors got 9 different expression plasmids which contain the synthetic IFN-γ-cDNA andhave different spaces between sd sequence and *** free energies Gf2980 in the formation of stable secondarystructure in the translation initiation region（TIR）are different in various expression *** of them,pLY4-γ5,can highly yield INF-γ which will be about 60%—80% of the total bacterial proteins,such a highexpression was hardly noted in *** reasons of high expression in this work are optimal spaces be-tween sd and ATC,favorable △Gf2980,favourable codons usage for ***.A simple and rapid method of purification of IFN-γ was *** purity of IFN-γ will be over95%,and the specific activity will be 2.0×107 IU/mg after islotation of inclusion body and one stepchromatography.

关键词： IFN-γ synthetic gene high expression.

Hydrolytic Enzyme Inhibitors and Necrotic Reactions in Potato Leaves Reduce Reproductive Success of Colorado Potato Beetle

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Journal of Agricultural Science and Technology(A) 2014年第4期4卷 331-341页

作者： Rinat Ismagilovich Ibragimov Ildar Salimyanovich Mardanshin Galina Vasilyevna Benkovskaya Maksim Borisovich Udalov Irina Andreevna Shpirnaya Vyacheslav Olegovich Tsvetkov Department of Biology Bashkir State University City of Ufa 450076 Republic of Bashkortostan Russia Laboratory of Potato Research Bashkir Scientific-Research Institute of Russian Agricultural Academy City of Ufa 450059 Republic of Bashkortostan Russia Laboratory of lnsects Adaptivity Biochemistry Institute of Biochemistry and Genetics USC RAS City of Ufa 450095 Republic of Bashkortostan Russia

In field and laboratory conditions the reproductive potential (survival at different stages of development) of the Colorado potato beetle has been measured, the dependence of the parameters of insects fertility on varietal features of the feed has been investigated, the food activity of the representatives of insects from four areas of the Southern Urals has been measured, the intensity and time of hypersensitivity reactions on potato leaves has been defined. In laboratory experiments, the level of activity of hydrolytic enzymes in insects and the level of activity of these enzymes inhibitors in the leaves of three potato varieties differing in their resistance to the Colorado potato beetle have been determined. The results show that the insects-representatives of different samples have different food activity in relation to different potato varieties. High mortality rates were revealed at feeding with leaves of potato of the Bashkir variety characterized by a high level of hydrolases inhibitors and relatively rapid development of necrotic reactions after the appearance of eggs laying on the leaves. We suppose that the necrogenetic barrier appearing in the form of necrosis is the major cause of the fetal mortality of insects. The level of the content of inhibitors inactivating larvae digestive enzymes affects the survival of insects at postembryonic stages. The speed of the necrosis emergence, the level of activity of digestive enzymes inhibitors in potato can be considered as criteria for the selection of breeding material for resistance to the Colorado potato beetle.

关键词：马铃薯甲虫酶抑制剂科罗拉多过敏反应繁殖潜力水解酶坏死实验室条件

Exendin-4 attenuates atherosclerosis progression via controlling hematopoietic stem/progenitor cell proliferation

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Journal of Molecular Cell Biology 2023年第2期15卷 43-57页

作者： Cen Yan Xiaojuan Ma Sin Man Lam Yuejie Zhang Yu Cao Yuan Dong Li Su Guanghou Shui Yingmei Feng Department of Science and Development Beĳing Youan hospitalCapital Medical UniversityBeĳing 100069China Center of Basic Medical Research Institute of Medical Innovation and ResearchPeking University Third HospitalBeĳing 100191China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeĳing 100101China Neuroscience Research institute Peking University Center of Medical and Health AnalysisPeking UniversityBeĳing 100191China

Beyond glycemic control, applications of glucagon-like peptide-1 receptor (GLP-1r) agonists (GLP-1 ras) inhibit inflammationand plaque development in murine atherosclerotic models. However, whether they modulate hematopoietic stem/progenitor cells(HSPCs)to prohibit skewed myelopoiesis in hypercholesteremia remains unknown. In this study, GLP-1r expression in fluorescenceactivated cell sorting (FACS)-sorted wild-type HSPCs was determined by capillary western blotting. Bone marrow cells (BMCs)of wild-type or GLP-1r−/− mice were transplanted into lethally irradiated low-density lipoprotein receptor deficient (LDLr−/−)recipients followed by high-fat diet (HFD) for chimerism analysis by FACS. In parallel, LDLr−/− mice were placed on HFD for 6weeks and then treated with saline or Exendin-4 (Ex-4) for another 6 weeks. HSPC frequency and cell cycle were analyzed byFACS, and intracellular metabolite levels were assessed by targeted metabolomics. The results demonstrated that HSPCs expressedGLP-1r and transplantation of GLP-1r−/− BMCs resulted in skewed myelopoiesis in hypercholesterolemic LDLr−/− *** vitro, Ex-4 treatment of FACS-purified HSPCs suppressed cell expansion and granulocyte production induced by LDL. In vivo, Ex-4treatment inhibited plaque progression, suppressed HSPC proliferation, and modified glycolytic and lipid metabolism in HSPCs ofhypercholesteremic LDLr−/− mice. In conclusion, Ex-4 could directly inhibit HSPC proliferation induced by hypercholesteremia.

关键词： glucagon-like peptide-1 receptor agonist hematopoietic stem/progenitor cells proliferation metabolomics atherosclerosis

Evaluation of biomarkers, genetic mutations, and epigenetic modifications in early diagnosis of pancreatic cancer

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World Journal of Gastroenterology 2021年第36期27卷 6093-6109页

作者： Bilal Rah Manzoor Ahmad Banday Gh rasool Bhat Omar J Shah Humira Jeelani Fizalah Kawoosa Tahira Yousuf Dil Afroze Advanced Centre for Human genetics Sher-i-Kashmir Institute of Medical SciencesSrinagar 190011Jammu and KashmirIndia Department of Medical Oncology Sher-i-Kashmir Institute of Medical SciencesSrinagar 190011Jammu and KashmirIndia Department of Surgical Gastroenterology Sher-i-Kashmir Institute of Medical SciencesSrinagar 190011Jammu and KashmirIndia Department of Immunology and Molecular Medicine Sher-i-Kashmir Institute of Medical ScienceSrinagar 190011Jammu and KashmirIndia

BACKGROUND Pancreatic cancer(PC)is one of the deadliest malignancies with an alarming mortality *** significant advancement in diagnostics and therapeutics,early diagnosis remains elusive causing poor prognosis,marred by mutations and epigenetic modifications in key genes which contribute to disease *** To evaluate the various biological tumor markers collectively for early diagnosis which could act as prognostic biomarkers and helps in future therapeutics of PC in Kashmir *** A total of 50 confirmed PC cases were included in the study to evaluate the levels of carbohydrate antigen 19-9(CA 19-9),tissue polypeptide specific antigen(TPS),carcinoembryonic antigen(CEA),vascular endothelial growth factor-A(VEGF-A),and epidermal growth factor receptor(EGFR).Mutational analysis was performed to evaluate the mutations in Kirsten rat sarcoma(Kras),Breast cancer type 2(BRCA-2),and deleted in pancreatic cancer-4(DPC-4)***,epigenetic modifications(methylation of CpG islands)were performed in the promoter regions of cyclin-dependent kinase inhibitor 2A(p16;CDKN2A),MutL homolog 1(hMLH1),and ras association domain-containing protein 1(rasSF1A)*** We found significantly elevated levels of biological markers CA 19-9(P≤0.05),TPS(P≤0.05),CEA(P≤0.001),and VEGF(P≤0.001).Molecular genetic analysis revealed that Kras gene mutation is predominant in codon 12(16 subjects,P≤0.05),and 13(12 subjects,P≤0.05).However,we did not find a mutation in DPC-4(1203G>T)and BRCA-2(617delT)***,epigenetic modification revealed that CpG methylation in 21(P≤0.05)and 4 subjects in the promoter regions of the p16 and hMLH1 gene,*** In conclusion,CA 19-9,TPS,CEA,and VEGF levels were significantly elevated and collectively have potential as diagnostic and prognostic markers in *** data of mutation in the Kras gene commonly in codon 12 and rare in codon 13 could augment the predisposition towards ***,methylatio

关键词： Pancreatic cancer Genetic mutations Epigenetic modifications Biomarkers Risk factors Diagnostics

Improvement of nitrogen use efficiency and grain yield in rice

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Improvement of nitrogen use efficiency and grain yield in ri...