检索结果-南通市图书馆

An Uncanonical CCCH-Tandem Zinc-Finger Protein Represses Secondary Wall Synthesis and Controls Mechanical Strength in Rice

引用

Molecular Plant 2018年第1期11卷 163-174页

作者： Dongmei Zhang Zuopeng Xu Shaoxue Cao Kunling Chen Shance Li Xiangling Liu Caixia Gao Baocai Zhang Yihua Zhou State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China State Key Laboratory of Plant cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China University of Chinese Academy of Sciences Beijing 100049 China

Secondary walls, which represent the bulk of biomass, have a large impact on plant growth and adaptation to environments. Secondary wall synthesis is switched and regulated by a sophisticated signaling transduction network. However, there is limited understanding of these regulatory pathways. Here, we report that ILAl-interacting protein 4 （lIP4） can repress secondary wall synthesis, lIP4 is a phosphorylation sub- strate of an Raf-like MAPKKK, but its function is unknown. By generating lip4 mutants and relevant transgenic plants, we found that lesions in lIP4 enhance secondary wall formation. Gene expression and transactivation activity assays revealed that lIP4 negatively regulates the expression of MYB61 and CESAs but does not bind their promoters, lIP4 interacts with NAC29/NAC31, the upstream regulators of secondary wall synthesis, and suppresses the downstream regulatory pathways in plants. Mutagenesis analyses showed that phosphomimic UP4 proteins translocate from the nucleus to the cytoplasm, which releases interacting NACs and attenuates its repression function. Moreover, we revealed that liPs are evolutionarily conserved and share unreported CCCH motifs, referred to as uncanonical CCCH-tandem zinc-finger proteins. Collectively, our study provides mechanistic insights into the control of secondary wall synthesis and presents an opportunity for improving relevant agronomic traits in crops.

关键词： secondary wall synthesis regulatory pathway repressor zinc finger rice

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Creating Wheat Germplasm for High Quality Breeding by Monosomic Backcrossing

引用

Journal of Integrative Plant biology 2006年第10期48卷 1210-1215页

作者： Yan-Mei Wang Jun Ji Dao-Wen Wang Zheng-Bin Zhang Ai-Min Zhang Center for Agricultural Resources Research Institute of Genetic and Developmental Biology Academy of Sciences Shijiazhuang 050021 China National Key Laboratory of Plant cell and Chromosome Engineering Institute of Genetics and Developmental Biology the Chinese Academy of Sciences Beijing 100101 China Graduate School of the Chinese Academy of Sciences Beijing 100049 China

By crossing bread wheat cultlvar GC8901 with the 1D monosonlc line of Xiaoyan No. 6 and backcrosslng the offsprlng with the Xlaoyan No. 6 1D monosonlc llne for 5 years, high-molecular-welght glutenin subunlts 1Dx5＋1Dy10 from GC8901 have been transferred Into wheat cultivar Xiaoyan No. 6. The BC5F1 offspring lines had been detected by using methods of cytology, marker, molecular marker and six elite single plants with high molecular-welght glutenin subunlts： lAx1, 1Bx14＋1 By15, 1Dx5＋1 Dy10 were Identified. Those lines have high-yleld potential with better agronomic characters and have been used In high quality wheat breeding processes as well.

关键词： germplasm for high quality breeding high-molecular-weight glutenin subunits monosomic backcrossing wheat

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Multiple Signaling Pathways Control Tbx6 Expression during Xenopus Myogenesis

引用

Acta Biochimica et Biophysica Sinica 2004年第6期36卷 390-396页

作者： Pan-Feng FANG, Rui-Ying HU, Xing-Yue HE, and Xiao-Yan DING* Laboratory of Molecular and cell biology, Laboratory of Stem cell biology & institute of Biochemistry and cell biology, Shanghai institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China Laboratory of Molecular and cell biology Laboratory of Stem Cell Biology & Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200031 China

Tbx6 is critical for somite specification and myogenesis initiation. It has been shown that Activin/Nodal, VegT/Nodal, FGF, and BMP signaling pathways are involved early in specifying mesoderm or later in patterning mesoderm, and Xnot plays roles in setting up the boundary between notochord and paraxial mesoderm. In this study, we introduce the dominant negative form of above genes into embryos to evaluate if they are responsible for regulating Tbx6 expression. The results show that: (1) Activin/Nodal and VegT/Nodal signals are necessary for both initiation and maintenance of Tbx6 expression, and Nodal is sufficient to induce ectopic Tbx6 expression; (2) FGF signal is necessary for the initiation and maintenance of Tbx6, but it is not sufficient to induce Tbx6 expression; (3) BMP is also necessary for the expression of Tbx6, and the induction of Tbx6 expression by BMP is dose dependent; (4) Xnot has no effect on the expression of Tbx6. Our results suggest that several signaling pathways are involved in regulating Tbx6 expression, and pave the route to reveal the molecular mechanism of initiating myogenesis.

关键词： Tbx6 FGF BMP Activin/Nodal VegT/Nodal

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cytokinin signal transduction:Known simplicity and unknown complexity

引用

Chinese Science Bulletin 2003年第13期48卷 1309-1315页

作者： ZHENG Binglian, SUN Jiaqiang, ZHANG Suzhi, DENG Yan & ZUO Jianru institute of Genetics and developmental biology, Chinese Academy of Sciences, Beijing 100101, China Correspondence should be addressed to Zuo Jianru (e-mail: jrzuo@ ***) institute of Genetics and developmental biology Chinese Academy of Sciences Beijing China

Cytokinin plays a critical role in plant growth and development by regulating cell divisions and cell differentiation. Recent studies suggest that cytokinin signaling is presumably mediated by a two-component system analogous to those found in bacteria and fungi, which transduces an external signal via a phosphorelay from the plasma membrane-anchored receptors to downstream effectors and regulators. Moreover, cytokinin signaling is highly interactive with other pathways, and many components of the pathway appear to be functionally redundant. Proper address of these questions will be crucial for our further understanding on this important network.

关键词：细胞分裂素信号转换植物生长基因突变

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Patterning the embryo in higher plants:Emerging pathways and challenges

引用

Frontiers in biology 2011年第1期6卷 3-11页

作者： Peng ZHAO Dong-Qiao SHI Wei-Cai YANG Key Laboratory of Molecular and developmental biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

Embryogenesis,which establishes the basic body plan for the post-embryonic organs after stereotyped cell divisions,initiates the first step of the plant life *** in the last two decades indicate that embryogenesis is a precisely controlled process,and any defect would result in *** we discuss the recent progresses,with a focus on the cellular pathways governing early embryogenesis in the model species Arabidopsis.

关键词： Arabidopsis embryogenesis auxin signaling receptor-like kinases(RLKs) non-cell-autonomous transcription factors and microRNA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Current advances in genome sequencing of common wheat and its ancestral species

引用

The Crop Journal 2018年第1期6卷 15-21页

作者： Xiaoli Shi Hong-Qing Ling State Key Laboratory of Plant cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences College of Life Sciences University of Chinese Academy of Sciences

Common wheat is an important and widely cultivated food crop throughout the *** progress has been made in regard to wheat genome sequencing in the last *** from the sequencing of single chromosomes/chromosome arms whole genome sequences of common wheat and its diploid and tetraploid ancestors have been decoded along with the development of sequencing and assembling technologies. In this review, we give a brief summary on international progress in wheat genome sequencing, and mainly focus on reviewing the effort and contributions made by Chinese scientists.

关键词： Genome sequencing Diploid wheat Tetraploid wheat Triticum aestivum

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Control of Grain Size and Weight by the OsMKKK10-OsM KK4-OsMAPK6 Signaling Pathway in Rice

引用

Molecular Plant 2018年第6期11卷 860-873页

作者： Ran Xu Penggen Duan Haiyue Yu Zhengkui Zhou Baolan Zhang Ruci Wang Jing Li Guozheng Zhang Shangshang Zhuang Jia Lyu Na Li Tuanyao Chai Zhixi Tian Shanguo Yao Yunhai Li State Key Laboratory of Plant cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China CAS Centre for Excellence in Molecular Plant biology Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China State Key Laboratory of Plant Genomics National Centre for Plant Gene Research (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100039 China

Grain size is one of the key agronomic traits that determine grain yield in crops. However, the mechanisms underlying grain size control in crops remain elusive. Here we demonstrate that the OsMKKK10-OsMKK4- OsMAPK6 signaling pathway positively regulates grain size and weight in rice. In rice, loss of OsMKKKIO function results in small and light grains, short panicles, and semi-dwarf plants, while overexpression of constitutively active OsMKKK10 （CA-OsMKKK10） results in large and heavy grains, long panicles, and tall plants. OsMKKK10 interacts with and phosphorylates OsMKK4. We identified an OsMKK4 gain-of-func- tion mutant （large11-1D）that produces large and heavy grains. OsMKK4A227T encoded by the large11-1D allele has stronger kinase activity than OsMKK4. Plants overexpressing a constitutively active form of OsMKK4 （OsMKK4oDD） also produce large grains. Further biochemical and genetic analyses revealed that OsMKKK10, OsMKK4, and OsMAPK6 function in a common pathway to control grain size. Taken together, our study establishes an important genetic and molecular framework for OsMKKK10-OsMKK4- OsMAPK6 cascade-mediated control of grain size and weight in rice.

关键词： Oryza sativa grain size MAPK signaling cell proliferation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Expression pattern of a β_2 tubulin gene in zebrafish embryos identified by whole mount in situ hybridization

引用

Chinese Science Bulletin 1998年第22期43卷 1894-1898页

作者： LI Shuhong, GUO Shaodong and SUN Fangzhen institute of developmental biology, Chinese Academy of Sciences, Beijing 100080, China institute of developmental biology Chinese Academy of Sciences Beijing China

Zebrafish is a good vertebrate model for development studies. Research on gene expression and regulation during zebrafish embryonic development plays an important role in revealing the molecular mechanism of morphogenesis in vertebrate. Tubulin locates the cytoplasmic determinants in eggs, organizes the formation of basic cytoskeleton and controls various cell movements. There are several types of tubulin, each of which has specific expression regions and functions. In this study, the full_length cDNA of a Xenopus β 2 tubulin was used as template to generate Dig_labeled antisense RNA which was used as a probe to carry out in situ hybridization on whole mount zebrafish embryos. Under the experimental conditions, the tubulin transcript was first detected in a group of cells in embryos at the midblastula stage. With the development of the embryo, this transcript was gradually restricted to the nervous system. These results demonstrate that the expression of this β 2 tubulin gene in zebrafish embryos is neural specific.

关键词： tubulin gene whole mount in situ hybridization zebrafish.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cloning and analysing of 5' flanking region of Xenopus organizer gene noggin

引用

cell Research 1999年第3期9卷 209-216页

作者： TAO QIN HUA JING YANG WEN YAN MEI XlNGENG XIAO YAN DING(Shanghai institute of cell biology, CAS, Shanghai 200031,China) Shanghai institute of cell biology CAS Shanghai China

Xenopus organizer specific gene Noggin possessesnearly all the characterestic properties of the action of or-ganizer to specify the embryonic body axis. To analyzehow the maternal inherited factors cofltrol its expressionpattern, we cloned the 5' regulatory region of noggn *** 1.5 kb upstream sequense could direct reporter geneto express in vivo and data from deletion analysis indi-cated that a 229 base pair fragmet is essential for acti-vating noggn expression. We further demonstrated thatthe response elements within this regulatory region wereindeed under the control of growth factor activin and Wntsignaling pathway components.

关键词： Xenopus organizer specific gene noggin regulatory region

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

SWR1 Chromatin-Remodeling Complex Subunits and H2A.Z Have Non-overlapping Functions in Immunity and Gene Regulation in Arabidopsis

引用

Molecular Plant 2016年第7期9卷 1051-1065页

作者： Souha Berriri Sreeramaiah N Gangappa and S Vinod Kumar cell and developmental biology Department John Innes Centre Norwich NR4 7UH UK

Incorporation of the histone variant H2A.Z into nucleosomes by the SWR1 chromatin remodeling complex is a critical step in eukaryotic gene regulation. In Arabidopsis, SWRlc and H2A.Z have been shown to con- trol gene expression underlying development and environmental responses. Although they have been implicated in defense, the specific roles of the complex subunits and H2A.Z in immunity are not well under- stood. In this study, we analyzed the roles of the SWRlc subunits, PHOTOPERIOD-INDEPENDENT EARLY FLOWERING1 （PIE1）, ACTIN-RELATED PROTEIN6 （ARP6）, and SWR1 COMPLEX 6 （SWC6）, as well as H2A.Z, in defense and gene regulation. We found that SWRlc components play different roles in resistance to different pathogens. Loss of PIE1 and SWC6 function as well as depletion of H2A.Z led to reduced basal resistance, while loss of ARP6 fucntion resulted in enhanced resistance. We found that mutations in PIE1 and SWC6 resulted in impaired effector-triggered immunity. Mutation in SWRlc components and H2A.Z also resulted in compromised jasmonic acid/ethylene-mediated immunity. Genome-wide expres- sion analyses similarly reveal distinct roles for H2A.Z and SWRlc components in gene regulation, and sug- gest a potential role for PIE1 in the regulation of the cross talk between defense signaling pathways. Our data show that although they are part of the same complex, Arabidopsis SWRlc components could have non-redundant functions in plant immunity and gene regulation.

关键词： arabidopsis SWR1 chromatin remodeling H2A.Z immunity gene regulation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：