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Journal of Environmental Sciences 2008年第12期20卷 1508-1513页

作者： Pichiah Saravanan Kannan Pakshirajan Prabirkumar Saha Department of Chemical Engineering Indian Institute of Technology GuwahatiGuwahati Department of biotechnology Indian Institute of Technology GuwahatiGuwahati

An acclimatized mixed microbial culture,predominantly Pseudomonas sp.,was enriched from a sewage treatment plant,and its potential to simultaneously degrade mixtures of phenol and m-cresol was investigated during its growth in batch shake flasks.A 22 full factorial design with the two substrates at two different levels and different initial concentration ranges(low and high),was employed to carry out the biodegradation *** substrates phenol and m-cresol were completely utilized within 21 h when ...

关键词： biodegradation factorial design of experiments kinetics m-cresol mixed microbial culture phenol sum kinetics model

Mechanism of Action of Natural Products Used in the Treatment of Diabetes Mellitus

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Chinese Journal of Integrative Medicine 2011年第8期17卷 563-574页

作者： Pranav Kumar Prabhakar Mukesh Doble Department of biotechnology Indian Institute of Technology Madras

Diabetes mellitus（DM） is a metabolic disorder caused by insufficient or inefficient insulin secretary response and it is characterized by increased blood glucose levels（hyperglycemia）.DM is a heterogonous group of *** is the main energy source for the body,and in the case of DM,management of glucose becomes *** are three key defects in the onset of hyperglycemia in DM,namely increased hepatic glucose production,diminished insulin secretion, and impaired insulin *** drugs treat diabetes by improving insulin sensitivity,increasing insulin production and/or decreasing the amount of glucose in *** article provides a comprehensive review of the mode of action of most popular hypoglycemic herbs,such as ginseng,bitter melon,fenugreek,banaba, Gymnema sylvestre and Coptis *** herbs act by increasing insulin secretion,enhancing glucose uptake by adipose and skeletal muscle tissues,inhibiting intestinal glucose absorption and inhibiting hepatic glucose *** evidence from animals and humans consistently supports the therapeutic effect of these phytomedicines,multicenter large-scale clinical trials have not been conducted to evaluate the safety and efficacy of these herbal medicines and their interaction with conventional drugs when administered simultaneously.

关键词： hyperglycemia medicinal plants ginseng bitter melon fenugreek banaba

Biosensors: Types, features, and application in biomedicine

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Asian Pacific Journal of Tropical Biomedicine 2022年第9期12卷 367-373页

作者： Elmira Karami Fatemeh Kazemi-Lomedasht biotechnology Research Center Biotechnology DepartmentPasteur Institute of IranTehranIran

Fast and precise diagnostic techniques are required for the treatment of many *** are one of the diagnostic devices that are applicable in biological and medical *** could be utilized to recognize biological molecules with high *** are consisted of different components and have different *** type of biosensor is used in a particular field according to its specific *** are a novel class of antibodies with small size,high affinity,and specificity to their *** unique properties of nanobodies make them appropriate tools for diagnostic *** this paper,we review biosensors,and their features and roles in ***/nanobody-based biosensors are also specifically discussed.

关键词： Biosensor Nanobody-based biosensor Antibodybased biosensor Biomedicine biotechnology

Sequence analysis and expression pattern of MGTA1 gene in rice blast pathogen Magnaporthe grisea

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Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2005年第8期6卷 817-824页

作者：王教瑜刘小红卢建平林福呈 biotechnology institute School of Agriculture and Biotechnology Zhejiang UniversityHangzhou 310029 China Microbiology institute School of Life Sciences Zhejiang UniversityHangzhou 310029 China

MGTA1, a putative fungal Zn（Ⅱ）2Cys6 transcriptional activator-encoding gene, was isolated from rice blast pathogen Magnaporthe grisea, which is homologous to CLTA1 from Colletotrichum lindemuthianum with 51% identity at protein level. MGTA1 cassette contains a 2370 bp open reading frame, consisting of 6 exons, coding a 790 amino acid peptide. MGTA1 gene exists as a single copy in genomes of 7 strains of M. grisea, and is expressed in tip hyphae, conidia, and mature appressoria of strain Guy 11.

关键词： Magnaporthe grisea MGTA1 gene Zn(Ⅱ)2Cys6

Establishment of Smad2 conditional gene targeting mice based on the Cre-LoxP system

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Science China(Life Sciences) 2002年第2期45卷 129-137页

作者：周江黄培堂程萱黄翠芬孙彦洵杨晓 Genetic Laboratory of Development and Diseases Institute of Biotechnology Beijing China

Smads is a new gene family in transforming growth factor-β(TGF-β) signaling pathway. Smad2 mutated in multiple human tumors and may be a candidate tumor suppressor gene. Targeted disruption of murine Smad2 gene resulted in embryonic lethality at E6.5. To study the function of Smad2 in vertebrate organgenesis and tumorigenesis, we constructed the Smad2 conditional targeting vector in which two LoxP sequences were placed to flank the sequences encoding the C terminal functional domain of Smad2. The validity of the LoxP sites in the targeting construct was tested in E. coli that express the Cre recombinase constitutively. The vector was electroporated into ES cells and 3 targeted ES cell clones were obtained by Southern blot screening. Targeted ES cells were introduced into C57BL/6J blastocysts by microinjection to generate germ-line chimeras. Genotyping analysis showed that 2 progeny among these chimeras carried the Smad2 conditional targeted allele. The establishment of Smad2 conditional gene targeting mouse has laid a solid foundation for producing the tissue specific Smad2 gene knockout mice.

关键词： Smad2, embryonic stem cell, Cre-LoxP system, conditional gene knockout.

Wound healing activity of Delonix elata stem bark extract and its isolated constituent quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside in rats

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Journal of Pharmaceutical Analysis 2016年第6期6卷 389-395页

作者： Pradeepa Krishnappa Krishna Venkatarangaiah Venkatesh Santosh Kumar Shimoga Rajanna Rebijith Kayattukandy Balan Department of Post Graduate Studies and Research in biotechnology Kuvempu University Department of biotechnology MS Ramaiah Institute of Technology Department of Biochemistry Indian Institute of Science(IISc) Division of biotechnology Indian Institute of Horticultural Research(IIHR)

Delonix elata *** a Ceasalpinaceae species and is traditionally used in India for treatment of skin diseases,liver diseases and rheumatic ***,systematic evaluation of its wound healing activity is ***,in the present study,we aimed to assess the wound healing activity of *** stem bark extract(DSE) and its isolated constituent quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside(QRPG) in *** formulations effects on wound healing were assessed by the wound contraction rate,epithelialization period,tensile strength,content of the hydroxyproline,hexosamine and uronic acid in granulation tissue,histopathological studies and Col 1 α(I) expression level in wound tissue by reverse transcription polymerase chain reaction(RT-PCR) *** topical application of DSE ointment caused faster epithelialization,significant wound contraction(100%),and better tensile strength(710.5 ± 10.5 g/cm^2),while QRPG showed wound epithelialization with 98.2%contraction,better than that of the control group(78.18%).The biochemical analysis of granulation tissue revealed that DSE and QRPG significantly increased hydroxyproline,hexosamine and uronic acid content.A significant increase in the expression of Col 1 α(I) was observed in the wound tissue of DSE and QRPG treated *** and QRPG were shown to enhance wound healing by increasing collagen synthesis through upregulation of Col 1 α(I),thus validating ethnomedicinal uses.

关键词： Delonix elata Quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside (QRPG) Wound healing Hydroxyproline Tensile strength

Comparing two approaches for introgression of germplasm from Aegilops tauschii into common wheat

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The Crop Journal 2017年第5期5卷 355-362页

作者： Thomas S.Cox Jizhong Wu Shuwen Wang Jin Cai Qiaofeng Zhong Bisheng Fu institute of Crop Germplasm and biotechnology Jiangsu Academy of Agricultural Sciences

Allelic diversity in the wild grass Aegilops tauschii is vastly greater than that in the D genome of common wheat(Triticum aestivum), of which Ae. tauschii is the source. Since the 1980 s,there have been numerous efforts to harness a much larger share of Ae. tauschii^ extensive and highly variable gene pool for wheat improvement. Those efforts have followed two distinct approaches: production of amphiploids, known as "synthetic hexaploids," between T. turgidum and Ae. tauschii,and direct hybridization between 丁. aestiuum and Ae. tauschii;both approaches then involve backcrossing to 丁. aestiuum. Both synthetic hexaploid production and direct hybridization have led to the transfer of numerous new genes into common wheat that confer improvements in many traits. This work has led to release of improved cultivars in China, the United States, and many other countries. Each approach to D-genome improvement has advantages and disadvantages. For example, production of synthetic hexaploids can incorporate useful germplasm from both T. turgidum and ***, thereby enhancing the A, B, and D genomes; on the other hand, direct hybridization rapidly restores the recurrent parent's A and B genomes and avoids incorporation of genes with adverse effects on threshability, hybrid necrosis, vernalization response, milling and baking quality, and other traits, which are often transferred when T. turgidum is used as a parent. Choice of method will depend in part on the type of wheat being developed and the target environment. However, more extensive use of the so-far underexploited direct hybridization approach is especially warranted.

关键词： Aegilops tauschii Direct hybridization Origin of wheat Synthetic hexaploid Triticum aestivum Wheat breeding

Establishment of murine Smad5 double knockout ES cells and the studies on their properties

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Science China Life Sciences 2001年第2期44卷 121-129页

作者：杨晓 ,孙彦洵 ,周江 ,黄培堂 ,黄翠芬 ,徐晓玲 ,李翠玲 ,GotayJessica ,陈林 ,邓初夏 institute of biotechnology Beijing China National institute of Diabetes Digestive and Kidney Diseases NIH Bethesda USA

Smad5 is an intracellular transducer of TGF-β signals. Targeted disruption of murine Smad5 gene resulted in embryonic lethal. To study the function of Smad5 in organgenesis, we generated Smad5 double knockout ES cells by homologous recombination. We deleted the neo gene of the Smad5 targeted ES cells using Cre-LoxP system. Smad5 double knockout ES cells were obtained by transfecting the targeted ES cells using the same targeting construct. The results of chimeric study showed that Smad5 might play an important role during the development of heart and neural tube. Smad5 double knockout ES cells formed teratoma when injected subcutaneously into nude mice. They differentiated into several types of cells, including neural cells, muscle cells, chondrocytes, endothelial cells and glandaceous cells. Smad5 double knockout ES cells are useful for studying the function of Smad5 mediated TGF- β during the organgenesis and the in vitro differentiation of ES cells.

关键词： Smad5 double knockout ES cells Cre-LoxP.

Endostatin promotes the anabolic program of rabbit chondrocyte

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Cell Research 2005年第3期15卷 201-206页

作者： Yi FENG,Yi Pin WU,Xu Dong ZHU,Yan Hong ZHANG,Qing Jun MA* Department of Molecule Genetics,Beijing institute of biotechnology,27 Taiping Road,Beijing 100850,China Department of Molecule Genetics Beijing Institute of Biotechnology Beijing China

Endostatin is a natural occurred angiogenesis inhibitor derived from collagenXVIII. So far its function during the angiogenesis process of bone formation and arthropathy has not been well studied yet. The present study addresses the function of endostatin in rabbit articular chondrocytes (RAC). We found that endostatin can promote RAC adhesion and spreading as well as its proliferation. In monolayer cultured RAC, CollagenII, TIMP1 and collagenXVIII transcription were up regulated by endostatin while collagenI and MMP9 were down regulated. Moreover collagenXVIII and endostatin antigens are present at synovial fluid. These findings indicate new function of endostatin as a homeostatic factor in cartilage metabolism.

关键词： endostatin angiogenesis chondrocyte matrix metalloproteinase.

Sulfide oxidation in fluidized bed bioreactor using nylon support material

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Journal of Environmental Sciences 2012年第3期24卷 512-519页

作者： Varsha Midha M K Jha Apurba Dey Department of Chemical Engineering National Institute of TechnologyJalandhar 144011India Department of biotechnology National Institute of Technology DurgapurIndia

A continuous fluidized bed bioreactor （FBBR） with nylon support particles was used to treat synthetic sulfide wastewater at different hydraulic retention time of 25, 50 and 75 min and upflow velocity of 14, 17 and 20 m/hr. The effects of upflow velocity, hydraulic retention time and reactor operation time on sulfide oxidation rate were studied using statistical model. Mixed culture obtained from the activated sludge, taken from tannery effluent treatment plant, was used as a source for microorganisms. The diameter and density of the nylon particles were 2-3 mm and 1140 kg/m3, respectively. Experiments were carried out in the reactor at a temperature of （30 ± 2）°C, at a fixed bed height of 16 cm after the formation of biofilm on the surface of support particles. Biofilm thickness reached （42 ± 3） μm after 15 days from reactor start-up. The sulfide oxidation, sulfate and sulfur formation is examined at all hydraulic retention times and upflow velocities. The results indicated that almost 90%-92% sulfide oxidation was achieved at all hydraulic retention times. Statistical model could explain 94% of the variability and analysis of variance showed that upflow velocity and hydraulic retention time slightly affected the sulfide oxidation rate. The highest sulfide oxidation of 92% with 70% sulfur was obtained at hydraulic retention time of 75 min and upflow velocity of 14 m/hr.

关键词： biofilm fluidized bed bioreactor mixed culture sulfide oxidation